ambr® 15 fermentation

New Microbial Single-Use

Advanced Micro Bioreactor System
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A New Version of the Industry
Standard ambr® 15 Micro Bioreactor,
for Microbial Screening Applications
ambr 15 fermentation is an automated microscale bioreactor system that
is based on the gold standard ambr 15 technology. It provides a consistent
microscale model for early stage microbial screening experiments with
fed-batch culture capability. This benchtop system comprises disposable
micro bioreactor vessels, an automated workstation and user friendly
software. It offers automated parallel processing and control of 24 bioreactor
experiments by one operator.

Designed for installation in a standard laminar airflow biological safety cabinet

for aseptic operation, ambr 15 fermentation provides efficient, consistent and
predictive results compared to classical laboratory bioreactors.

ambr 15 fermentation transforms the way scientists undertake microbial

strain selection and early process development in the 21st century

- Increases lab productivity - Enables data rich culture profiles

through automation of experiment with automated sampling and enough
setup, sampling and reagent addition, culture volume for many samples during
for up to 24 experiments in parallel. a run.

- Enhances scalability over shaken - Reduces the cost per experiment

cultures
with control of DO & pH in fed-batch
culture, impeller stirring, gas sparging
and pumped additions of pH reagent
and feed.
- Provides predictive screening results
early in development
enabling the best early decisions and
improving the overall efficiency and
timeline of development process.
through savings on facility space,
capital, labour, media and consumables.
- Provides highly consistent data
as proven by the industry standard
‘ambr 15 cell culture’ micro bioreactor
platform, now implemented at 90% of
the top 20 biopharma.

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Features
ambr 15 fermentation combines disposable micro bioreactor vessels,
automated workstation and easy-to-use software

Culture stations
Each culture station can run up to 12 micro
bioreactor vessels and controls temperature
and impeller speed. Gas mixture is delivered
independently to each vessel.

Pumped addition of feed and pH reagents

Pump and tubing provide controlled
delivery of feed and pH reagents, enabling
high density fed-batch cultures with
precise pH control.

Image shows ambr 15 fermentation

24 vessel system

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 3 4
Individual vessel temperature control
Sensors and heaters for each vessel provide
a steady, uniform culture temperature,
even with a range of growth rates and heat
outputs in different vessels.
Liquid handling unit
For media, feed and reagent addition plus
sampling from the bioreactor vessels. Unit
includes dual pipette heads, automated
vessel decapper and plate delidder.
6 7
Labware lid management
Lids are automatically removed prior to
liquid transfer and replaced afterwards.
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Disposable tips
Two sizes of high grade sterile disposable
tips are available to transfer liquid volumes
accurately and precisely.
Chilled plate position
Chilled storage of media or culture samples
provides flexibility in scheduling of user
interactions. Chilled sample storage
facilitates data rich culture profiles with
multiple samples during a run.
Software
Software enables easy experiment
construction and controls and monitors all
experiments, with full audit trail.
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Functions
ambr 15 fermentation automatically controls, feeds and samples
24 bioreactor vessels in parallel

ambr 15 fermentation ambr 15 fermentation

– system – micro bioreactor vessel
Fully automated parallel processing, Mimics the characteristics of a classical lab
control and evaluation of all bioreactor scale bioreactor to enable good early stage
experiments with: predictions of cell growth and productivity:

–O
 nline monitoring and closed-loop
control of pH (6-8) and DO.
–C
 ascade DO control system for gas flow,
stirrer speed and gas mixture.
– Independent control of N2, air and O2
for each vessel.
– Full control of impeller speed, gas flow
rates and bioreactor temperature.
–p  H and DO sensors for continuous
monitoring and control (pH 6-8).
– Integral impeller for rapid, efficient
mixing.
– S parge tube delivers gas to the impeller
mixing zone.
–1  0 to 15 mL working volume (8 to 12 mL
liquid plus gas bubbles) with sample port
to allow addition of reagents & feeds or
removal of samples.

4 2 1

Micro bioreactor vessel

The core disposable micro bioreactor 5
technology includes sensors, stirring
impeller, gas sparging, fluid additions and
sample port.

1. Gas sparge tube

2. pH and feed tubes
3. Sample port
4. pH and DO sensor spots
5. Impeller

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Applications
ambr 15 fermentation can be used as a
microscale model to enhance results in a range
of development applications such as:

- Vector screening
- Strain selection
- Media development

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Sales and Service Contacts
For further contacts, visit www.sartorius-stedim.com
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Sartorius Stedim (Shanghai) Publication No. : SBI1542-e150601 Order No. : 85037-550-85 Ver. 04 | 2016
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