MIDTERM EXAM

Submitted by aid262362 on 6/17/2010 11:45:35 AM

Points Awarded 44
Points Missed 6

Percentage 88%

1. In Pasteur's swan neck flask experiment, the importance of the s-shaped curves in
the flasks was two-fold. The curves allowed entry of the air, and yet excluded

A. Nutrients.
B. Heat.
C. Maggots.
D. Bacteria.
E. Broth.
Points Earned: 1/1
Correct Answer: D
Your Response: D

2. Pasteurization was first developed to kill _______ in

wine.

A. Most bacteria.
B. All bacteria.
C. Antibiotic producing bacteria.
D. Disease causing bacteria.
E. Endospore producing bacteria.
Points Earned: 1/1
Correct Answer: A
Your Response: A

3. An exposure to ______ protects against disease after exposure to small

pox.

A. Normal microbiota
B. Chemotherapy
C. Penicillin
D. Cowpox
E. Antibody
Points Earned: 1/1
Correct Answer: D
Your Response: D
According to Carl Woese, all life can be classified into three
4.
groups:
A. Animalae, Plantae, Microbes
 B. Eukaryotes, Prokaryotes, viruses
C. Bacteria, Fungi, Protozoa
D. Bacteria, Archeae, Eukarya
E. Carnivores, Herbivores, Omnivores
Points Earned: 1/1
Correct Answer: D
Your Response: D

5. Protozoa are classified by their

A. Cell wall structure.

B. Mode of reproduction
C. Mechanism for deriving nutrients.
D. Shape.
E. Means of locomotion
Points Earned: 1/1
Correct Answer: E
Your Response: E

6. Using microbes to clean up pollutants is

called:

A. Bioremediation
B. Genetic engineering.
C. Gene therapy.
D. Nitrogen fixation
E. Fermentation
Points Earned: 1/1
Correct Answer: A
Your Response: A

7. Which is not a characteristic of the normal

microbiota?

A. Often are called the Normal Flora.

B. Regularly associated with disease symptoms.
C. Live within our bodies.
D. May benefit the human host.
E. Live on our bodies.
Points Earned: 1/1
Correct Answer: B
Your Response: B

8. In the scientific name Escherichia coli, Escherichia is

the

A. Genus
B. Family
C. Species
 D. Class
Points Earned: 1/1
Correct Answer: A
Your Response: A

9. Which of the following scientists and their discoveries are incorrectly

matched

A. Koch ? germ theory of disease

B. Erlich ? chemotherapy
C. Jenner ? vaccination
D. Lister - antibiotics
Points Earned: 1/1
Correct Answer: D
Your Response: D

10. Development of tests to identify Hantavirus showed that this

virus

A. also caused Creutzfeld -Jacob Syndrome.

B. was responsible for bloody diarrhea syndrome.
C. is associated with AIDS.
D. is similar to the invasive group A Streptococcus.
E. came to North America with the first rats from the Old World.
Points Earned: 1/1
Correct Answer: E
Your Response: E

11. You have isolated a cell that lacks a membrane-enclosed nucleus. You would
find all of the following structures except which one

A. A ribosome
B. Peptidoglycan
C. A chromosome
D. A lysosome
Points Earned: 1/1
Correct Answer: D
Your Response: D

12. Which one of the following mechanisms requires

energy

A. Simple diffusion
B. Active diffusion
C. Active transport
D. Osmosis
Points Earned: 1/1
Correct Answer: C
Your Response: C
 13. Which of the following inclusions stores an
enzyme

A. polysaccharide granules
B. lipid inclusions
C. Carboxysomes
D. metachromatic granules
Points Earned: 1/1
Correct Answer: C
Your Response: C

14. All of the following statements are true about gram-positive cells when
compared to gram-negative cells except which one

A. gram-positive cells have more peptidoglycan

B. gram-positive cells are decolorized by alcohol-acetone
C. gram-positive cells have teichoic acids
D. gram-positive cells are sensitive to penicillin
Points Earned: 1/1
Correct Answer: B
Your Response: B

15. Pseudomurein is found in the cell walls

of

A. gram-positive bacteria
B. Mycoplasmas
C. archea
D. gram-negative bacteria
Points Earned: 0/1
Correct Answer: C
Your Response: A

16. All of the following are produced naturally by microorganisms except which
one:

A. Silk
B. Acetone
C. Butyl alcohol
D. Ethyl alcohol
Points Earned: 1/1
Correct Answer: A
Your Response: A

17. Which of the following is not part of an enzymatic

action

A. enzyme is altered
B. products leave enzyme
C. enzyme ? substrate forms
 D. substrate contacts active site
Points Earned: 1/1
Correct Answer: A
Your Response: A

18. Which of the following four stages of glucose oxidation produces the most
ATP

A. glycolysis
B. production of acetyl CoA
C. Krebs cycle
D. oxidative phosphorylation
Points Earned: 1/1
Correct Answer: D
Your Response: D

19. During the dark reaction of photosynthesis,

organisms

A. ferment glucose
B. use molecular oxygen to produce ATP
C. produce molecular oxygen
D. synthesize sugars
Points Earned: 1/1
Correct Answer: D
Your Response: D

20. You work for a chemical company and you are responsible for growing a
bacterial culture that produces acetone. The bacteria are growing well in a
glucose medium but are not producing acetone. The most likely reason is

A. The bacteria are dead

B. Molecular oxygen is in the medium
C. The cells cannot use the glucose
D. The cells are fermenting the glucose
Points Earned: 1/1
Correct Answer: B
Your Response: B

21. Which of the following enzymes and their function are mis-
matched

A. DNA polymerase -- proofreads DNA

B. DNA polymerase -- makes DNA
C. RNA polymerase -- makes DNA
D. DNA ligase -- joins pieces of DNA
Points Earned: 1/1
Correct Answer: C
Your Response: C
22. Your lab partner has mixed a dead tryptophan+ strain of Bacillis subtilis with a
live tryptophan- strain and observes that her B. subtilis culture is now
tryptophan+. The most likely explanation for this is

A. Conjugation
B. transduction
C. transformation
D. mutation
Points Earned: 0/1
Correct Answer: C
Your Response: B

23. All of the following are examples of biotechnology except which

one:

A. Bread making with fungi and bacteria

B. Antibiotic production by bacteria
C. Enzyme production by bacteria and fungi
D. Bacteria that cause diseases
Points Earned: 1/1
Correct Answer: D
Your Response: D

24. Which of the following can be used as vectors in genetic

engineering

A. plasmids
B. Ti plasmid of Agrobacterium
C. viruses
D. all of the above
Points Earned: 1/1
Correct Answer: D
Your Response: D

25. Southern blotting is used to

identify

A. RNA in a cell
B. antibiotic resistance in a cell
C. a protein in a cell
D. DNA in a cell
Points Earned: 1/1
Correct Answer: D
Your Response: D

26. In order to express a human gene in a bacterium, cDNA must be made

because bacteria

A. usually destroys human DNA

B. usually cannot remove introns
 C. splice RNA
D. have reverse transcriptase
Points Earned: 1/1
Correct Answer: B
Your Response: B

27. All of the following are true about archaea except

A. They are prokaryotes.

B. They lack peptidoglycan in their cell walls.
C. Some are thermoacidophiles; others are extreme halophiles.
D. They evolved before bacteria.
E. Some produce methane from carbon dioxide and hydrogen.
Points Earned: 1/1
Correct Answer: D
Your Response: D

28. A bacterial species differs from a species of eukaryotic organisms in that a

bacterial species

A. Breeds with other species.

B. Has a limited geographical distribution.
C. Can be distinguished from other bacterial species.
D. Is a population of cells with similar characteristics.
E. All of the above are true.
Points Earned: 0/1
Correct Answer: D
Your Response: E

29. Protist is a diverse group of organisms that are similar

in

A. rRNA sequences.
B. Metabolic type.
C. Motility.
D. Ecology.
E. None of the above.
see p 284. Protists are eukaryotes that don't fit in other kingdoms.
Points Earned: 1/1
Correct Answer: A
Your Response: A

30. Bacteria and archaea are similar in which of the

following?

A. Peptidoglycan cell walls.

B. Methionine is the start signal for protein synthesis.
C. Sensitive to antibiotics
D. Possess prokaryotic cells.
E. None of the above.
 Points Earned: 0/1
Correct Answer: D
Your Response: B

31. A clone is

A. Genetically identical cells derived from a single cell.

B. A genetically engineered cell.
C. A taxon composed of species.
D. A mound of cells on an agar medium.
E. None of the above.
Points Earned: 1/1
Correct Answer: A
Your Response: A

32. A gene is best defined as

A. A segment of DNA.
B. Three nucleotides that code for an amino acid
C. A sequence of nucleotides in DNA that codes for a functional product
D. A sequence of nucleotides in RNA that codes for a functional product
E. A transcribed unit of DNA
Points Earned: 1/1
Correct Answer: C
Your Response: C
Which of the following pairs is mismatched?
33.

A. DNA polymerase  makes a molecule of DNA from a DNA template

B. RNA polymerase  makes a molecule of RNA from an RNA template
C. DNA ligase  joins segments of DNA
D. Transposase  insertion of DNA segments into DNA
E. DNA gyrase  relaxes coils in DNA
Points Earned: 1/1
Correct Answer: B
Your Response: B

34. Which of the following statements is false?

A. DNA polymerase joins nucleotides in one direction only.

B. The leading strand of DNA is made continuously.
C. The lagging strand of DNA is started by an RNA primer
D. DNA replication proceeds in one direction around the bacterial
chromosome.
E. Multiple replication forks are possible on a bacterial chromosome.
Points Earned: 1/1
Correct Answer: D
Your Response: D

35. Which of the following is not a product of

 transcription?

A. A new strand of DNA

B. rRNA
C. tRNA
D. mRNA
E. None of the above None of the above
Points Earned: 1/1
Correct Answer: A
Your Response: A

36. An enzyme produced in response to the presence of a substrate is

called

A. An inducible enzyme.
B. A repressible enzyme.
C. A restriction enzyme.
D. An operator.
E. A promoter.
Points Earned: 1/1
Correct Answer: A
Your Response: A

37. Transformation is the transfer of DNA from a donor to a recipient

cell

A. By a bacteriophage
B. As naked DNA in solution
C. By cell-to-cell contact
D. By crossing over
E. By sexual reproduction
Points Earned: 1/1
Correct Answer: B
Your Response: B

38. Genetic change in bacteria can be brought about

by

A. Mutation
B. Conjugation
C. Transduction
D. Transformation
E. All of the above
Points Earned: 1/1
Correct Answer: E
Your Response: E

39. Which of the following is not true of a bacterium that is

R+?
 A. R+ refers to the possession of a plasmid
B. R+ can be transferred to a recipient cell
C. It is resistant to certain drugs and heavy metals
D. It is F+
E. None of the above
Points Earned: 1/1
Correct Answer: D
Your Response: D

40. The damage caused by ultraviolet radiation

is

A. Never repaired
B. Repaired during transcription
C. Repaired during translation
D. Cut out and replaced
E. Repaired by DNA replication
Points Earned: 1/1
Correct Answer: D
Your Response: D

41. The necessary ingredients for DNA synthesis can be mixed together in a test
tube. The DNA polymerase is from Thermus aquaticus and the template is from
a human cell. The DNA synthesized would be most similar to

A. Human DNA
B. T. aquaticus DNA
C. A mixture of human and T. aquaticus DNA
D. Human RNA
E. T. aquaticus RNA
Points Earned: 1/1
Correct Answer: A
Your Response: A

42. Sar 11 is

A. a bacterium found in deep sea vents

B. a virus found in the ocean
C. a bacterium found in the ocean that can photosynthesize and is easy to
culture
D. a rare bacterium found in the ocean
E. a common bacterium found in the ocean that is difficult to culture
Points Earned: 0/1
Correct Answer: E
Your Response: A
Select 8

43. Which of the following is not a domain in the three-domain system? (Note: there are
only choices A-D for this question.)

A. Archaea
B. Bacteria
C. Eukarya
D. Animalia
Points Earned: 1/1
Correct Answer: D
Your Response: D

44. You have isolated a gram-positive rod. What should you do

next?

A. Gram stain
B. Lactose fermentation
C. Endospore stain
D. Flagella stain
E. Enterotube
Points Earned: 1/1
Correct Answer: C
Your Response: C

45. Which of the following do you expect to be most resistant to high

temperatures?

A. Bacillus subtilis
B. Eschericia coli
C. Neisseria gonorrhoeae
D. Staphylococcus aureus
E. Streptococcocus pyogenes
Points Earned: 1/1
Correct Answer: A
Your Response: A

46. The reaction catalyzed by reverse

transcriptase

A. DNA → mRNA
B. mRNA → cDNA
C. mRNA → protein
D. DNA → DNA
E. None
Points Earned: 1/1
Correct Answer: B
Your Response: B

47. In bacteria, photosynthetic pigments are found

in
 A. Chloroplasts
B. Chromatophores
C. Cytoplasm
D. Mesosomes
E. None of the above
Points Earned: 1/1
Correct Answer: B
Your Response: B

48. Which of the following is not a structure found in prokaryotic

cells?

A. Pili
B. Axial filament
C. Flagella
D. Peritrichous flagella
E. Cilia
Points Earned: 0/1
Correct Answer: E
Your Response: D

49. A eukaryote used in genetic engineering

is

A. Agrobacterium tumefaciens
B. Thermus aquaticus
C. Saccharomyces cerevisiae
D. Bacillus thuringiensis
E. Pseudomonas
Points Earned: 1/1
Correct Answer: C
Your Response: C

50. Which of the following is not a chemical component of a bacterial cell

wall?

A. N-acetylmuramic acid
B. Peptide chains
C. Peptidoglycan
D. Teichoic acids
E. Cellulose
Points Earned: 1/1
Correct Answer: E
Your Response: E