Name: 2018_VIIB_155070101111073_SITI A

Exercise 8: Chemical and Physical Processes of Digestion: Activity 4: Assessing Lipase Digestion of Fat Lab Report

Pre-lab Quiz Results

You scored 100% by answering 4 out of 4 questions correctly.

1. Which of the following is/are true of bile?

You correctly answered: b. It works by a physical process.

2. The substrate used in this simulation is

You correctly answered: c. vegetable oil.

3. When fatty acids are liberated by lipase, the pH

You correctly answered: b. decreases.

4. One of the products of the chemical digestion of lipids is

You correctly answered: d. fatty acids.

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Experiment Results
Predict Question:
Predict Question: Which tube do you think will have the highest lipase activity?
Your answer : a. tube 1

Stop & Think Questions:

What does the pH measure?
You correctly answered: d. both lipase activity and fatty acid release

Why is it difficult to detect whether lipase is active in tube 5?

You correctly answered: d. The pH is already very low, so a decrease in pH might be difficult to detect.

Experiment Data:

Tube No. Reagent 1 Reagent 2 Reagent 3 Reagent 4 Time Temp. pH

1 Lipase Vegetable Oil Bile Salts pH 7.0 Buffer 60 37 6.21
2 Lipase Vegetable Oil Deionized pH 7.0 Buffer 60 37 7.00
Water
3 Lipase Deionized Water Bile Salts pH 9.0 Buffer 60 37 9.00
4 Deionized Water Vegetable Oil Bile Salts pH 7.0 Buffer 60 37 7.00
5 Lipase Vegetable Oil Bile Salts pH 2.0 Buffer 60 37 2.00
6 Lipase Vegetable Oil Bile Salts pH 9.0 Buffer 60 37 9.00

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Post-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.

1. What is the product of lipase hydrolysis?

You correctly answered: d. fatty acids

2. From your results, which pH is ideal for pancreatic lipase digestion?

You correctly answered: b. pH 7.0

3. Which tube confirms that there is no lipase in bile salts or vegetable oil?
You correctly answered: c. tube 4

4. From your results, where (in theory) would pancreatic lipase be active?
You correctly answered: d. mouth and pancreas

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Review Sheet Results
1. Explain why you can't fully test the lipase activity in tube 5.
Your answer:
Karena pH tabung 5 sudah terlalu rendah sehingga sulit untuk mendeteksi penurunan pH akibat produksi asam lemak

2. Which tube had the highest lipase activity? How well did the results compare with your prediction? Discuss possible
reasons why it may or may not have matched.
Your answer:
Sesuai dengan prediksi saya bahwa tabung 1 yang memiliki aktivitas tertinggi karena lipase aktif di pH yang mendekati pH
usus dan tabung 1 yang mendekati

3. Explain why pancreatic lipase would be active in both the mouth and the pancreas.
Your answer:
Enzim lipase aktivitasnya paling tinggi di lingkungan pH sekitar 7, mulut memiliki pH sekitar 7 dan usus halus sekitar 8 maka
lipase akan aktif di keduanya.

4. Describe the process of bile emulsification of lipids and how it improves lipase activity.
Your answer:
Gumpalan lemak akan dipisahkan menjadi droplet-droplet kecil oleh garam empedu melalui emulsifikasi sehingga
meningkatkan luas permukaan yang terpapar oleh enzim lipase.

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