Mitochondrial DNA Part B

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The complete mitochondrial genome of Gibbovalva

kobusi (Lepidoptera: Gracillariidae)

Lu Chen, Cheng-Qing Liao, Xing Wang & Shao-Xian Tang

To cite this article: Lu Chen, Cheng-Qing Liao, Xing Wang & Shao-Xian Tang (2019) The
complete mitochondrial genome of Gibbovalva�kobusi (Lepidoptera: Gracillariidae), Mitochondrial
DNA Part B, 4:2, 2769-2770, DOI: 10.1080/23802359.2019.1644550

To link to this article: https://doi.org/10.1080/23802359.2019.1644550

© 2019 The Author(s). Published by Informa

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Published online: 29 Jul 2019.

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MITOCHONDRIAL DNA PART B
2019, VOL. 4, NO. 2, 2769–2770
https://doi.org/10.1080/23802359.2019.1644550

MITOGENOME ANNOUNCEMENT

The complete mitochondrial genome of Gibbovalva kobusi

(Lepidoptera: Gracillariidae)
Lu Chena,b
, Cheng-Qing Liaoa,b
, Xing Wanga,b and Shao-Xian Tangc
a
College of Plant Protection, Hunan Agricultural University, Changsha, Hunan, China; bHunan Provincial Key Laboratory for Biology and
Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, Hunan, China; cCollege of Information Science and
Technology, Hunan Agricultural University, Changsha, Hunan, China

ABSTRACT ARTICLE HISTORY

In this study, the complete mitochondrial genome (mitogenome) of Gibbovalva kobusi was at first Received 24 May 2019
sequenced by high-throughput sequencing. As a circular DNA molecule, the complete mitogenome is Accepted 10 July 2019
15,717 bp in length (GeneBank accession number: MK956103) and consists of 13 protein-coding genes
KEYWORDS
(PCGs), 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes, and an AT-rich region. The
Gibbovalva kobusi;
nucleotide composition is A (41.0%), C (11.6%), G (7.9%), and T (39.5%). Based on the sequences of mitochondrial genome;
complete mitogenome from 11 species as ingroups and 3 superfamily Tineoidea species as outgroups, evolutionary position
the phylogenetic trees were constructed. The family Gracilariidae as a monophyletic clade is strongly
supported by the bootstrap value of 100%.

Gibbovalva kobusi belongs to Acrocercopinae in the family
Gracilariidae (Lepidoptera: Gracillarioidea) (Kawahara et al. 2017)
and mainly damages the leaves of the families Lauraceae and
Magnoliaceae (Bai and Li 2008; Xu et al. 2014; Bai et al. 2016;
De Prins and De Prins 2018). In this study, G. kobusi, while min-
ing for Magnolia officinalis and Liriodendron chinense
(Magnoliaceae), was collected from Tianpingshan Mountain
(29 N, 110 E, elevation of 1332 m) in Badagongshan National
Nature Reserve (Sangzhi County, Zhangjiajie City, Hunan
Province) and its complete mitochondrial genome (mitoge-
nome) was reported for the first time. All specimens and the
genomic DNA were deposited at the Insect Museum of Hunan
Agricultural University, Changsha City, Hunan Province, China.
We gained the complete mitogenome of G. kobusi and con-
structed a phylogenetic tree for understanding its phylogenetic
position in the superfamily Gracillarioidea and relationships with
other closely related groups.
The complete genome DNA was extracted from the larvae
(collection number: LCQ006) by using TaKaRa MiniBEST
Universal Genomic DNA Extraction Kit Ver.5.0 (Shiga
Prefecture, Kusatsu City, Japan) and was sequenced by using
high-throughput sequencing in Berry Genomics Corporation.
The assembly of sequence used IDBA in server. The gene
annotation was accomplished with ORF finder (https://www.
ncbi.nlm.nih.gov/orffinder/) and MITOS web server (http://
mitos.bioinf.uni-leipzig.de/index.py) (Bernt et al. 2013).
To construct the phylogenetic tree, the complete mitoge-
nomes of 11 species in the superfamilies Gracillarioidea,
Yponomeutoidea, and Gelechioidea and 3 species of the
superfamily Tineoidea as outgroups were used in the analy-
ses. The maximum likelihood (ML) method was performed by
MEGA 10 and the bootstrap analysis was set as 1000
pseudoreplicates.
The complete mitogenome of G. kobusi is a circular DNA
molecule of 15,717 bp in length (GeneBank accession num-
ber: MK956103) and consists of 13 protein-coding genes
(PCGs), 22 transfer RNA (tRNA) genes, 2 ribosomal RNA
(rRNA) genes, and an AT-rich region, in which 23 genes are
transcribed on the J strand and the remaining 14 are tran-
scribed on the N strand. The nucleotide composition is A
(41.0%), C (11.6%), G (7.9%), T (39.5%), and the AT nucleotide
content is 80.5%. There were 469 bp intergenic nucleotides
that were dispersed in between 12 pairs of neighboring
genes with their length varying from 1 to 319 bp. The length
of the A þ T rich region that was located between rrnS and
the trnM was 503 bp. The phylogenetic tree based on
sequences of the complete mitogenome was shown in
Figure 1. The relationship within this superfamily
Gracilarioidea that is a monophyletic clade is strongly sup-
ported by the bootstrap value of 100% and as a sister group
of the superfamily Yponomeutoidea is also supported by the
bootstrap value of 91%.

CONTACT Shao-Xian Tang tsx@hunau.edu.cn College of Information Science and Technology, Hunan Agricultural University, Changsha, Hunan
410128, China

These authors have contributed equally to this work.
ß 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use,
distribution, and reproduction in any medium, provided the original work is properly cited.
2770 L. CHEN ET AL.

Figure 1. Maximum-likelihood tree of evolutionary relationships Gibbovalva kobusi based on the complete mitogenomes of 14 Lepidopteran moths. All the specie-
s’accession numbers in this study are listed as below: Mesophleps albilinella NC 029811, Atrijuglans hetaohei KT581634, Pectinophora gossypiella KM225795,
Helcystogramma macroscopa KM386998, Prays oleae NC 025948, Plutella australiana NC 039687, Plutella xylostella NC 025322, Phyllonorycter froelichiella KJ5008048,
Phyllonorycter platani KJ508044, Cameraria ohridella KJ508042, Tineola bisselliella KJ508045, Clania veriegata NC 041112, Mahasena oolona, NC 036410.

Disclosure statement
The authors declare that there is no conflict of interest regarding the
publication. The authors also are responsible for the content and writing
of the paper.
Funding
This study was supported by the National Natural Science Foundation of
China [31772157].
References
Bai HY, Li HH. 2008. A review of the genus Gibbovalva (Lepidoptera:
Gracillariidae: Gracillariinae)from China. Orient Insects. 42:317–326.
Bai HY, Xu JS, Dai XH. 2016. Two new and one newly recorded species
of Gracillariidae from China (Lepidoptera). Zookeys. 559:139–150.
Bernt M, Donath A, Juhling F, Juling F, Externbrink F, Florentz C, Fritzsch
G, Putz J, Middendorf M, Stadier PF. 2013. MITOS: improved de novo-
metazoan mitochondrial genome annotation. Mol Phylogenet Evol.
69:313–319.
De Prins J, De Prins W. 2018. Global taxonomic database of Gracillariidae
(Lepidoptera). World wide web electronic publication [accessed 2018
November 17]. http://www.gracillariidae.net.
Kawahara AY, Plotkin D, Ohshima I, Lopez-vaamonde C, Houlihan PR,
Breinholt J, Kawakita A, Xiao L, Regier JC, Davis DR, et al. 2017. A
molecular phylogeny and revised higher-level classification for the
leaf-mining moth family Gracillariidae and its implications for larval
host-use evolution. Syst Entomol. 42:60–81.
Xu JS, Bai HY, Dai XH. 2014. Two new host plants and bionomics of
Gibbovalva kobusi. Forest Pest Dis. 33:5–7.