Declassified and Approved For Release 2012/05/10 : CiA-RDP88BO1125R000300 120005-6 me 612,004.424.5 BIOLOGICAL EFFECT OF MILLIMETER RADTORAVES Kiev VRCHESNOYE DELO in Russian No 3, 1977 pp 126-119 [articte by I, P, Zalyubovakaya, Hhertkov Stientific Research Institute of HMcrobielogy, Vaceines and Sera’ ineni Mechnikov] [next] Morphological, functional and biochendcal studies conducted Ye'honene ond aninals revesled that nillinster waves caused Ranges da the body manifested An structural alterations in She dein and internal organs, qualitative and quentitative iRdnges of the blood snd bone marsew composition and changes SE the consitionea reflex activity, tissue respiration, ac GEvity of enzynte participating in’the processes of thésue Supluation ana muciete metaboliom. ‘The degree of unfavor— cee Chtect of milidmeter waves depended on the duration of fhe vailaticn and intividual charasteristies of the organism, she ubiquitous propagation of radiowaves, ratio broalessting and televis Jee weiMihucing! to ahe appearance of a new physical factor =~ electromagnetic Ae coneratne cedio-frequchey range. In recent years it has been esteblished C chiowaves of different ranges have an unfavorable influence on the see rene “me Liverature data (A. G, Subbota, 1970; N. V. Tyaging 1972; Be Pe Movin, 1973: Me I. Yakovieva, 19733 Yue D. Dunansity et oly 1979) Aer that dong stay in'conditione of the effect of radiowaves (the ds end teeta arcane to change of the functions of the nervous, cerdiovasculer oe Paget eteas of the orgenien, with he development of a characteristic Fea reee Tymptans which permit speaking of « special nosological form of SovPLgE of Moeave disease (MN. Sadchikova, 1973). However, in the it~ Sseete There 4s slnost no information sbout the biological effect of redio Seater [ue oe tne nillimcter range, alvhough that range is widely used in Sere ei ona the question of Ste biological activity has acquired special sme gout of the precent investigations consisted in study of the phystological Tes Eidtncneel processes lying st the basis of the changes which occur in aaa re ata temlt of the effect of radiovavas jn the range of 5-8 m, at euGhsity of the flow of power of 1 milLivatt/en’. The Snvestigations ‘vere 2 ercteh on rate of the Wistar Line and mice of the CBA Line, irradiated Sends on resciiy in the touree of 60 days in the volun resonator of an TMhervomneal installation working on the hasis of « type OV-12 generator, a 105/10 - CiA-ROPS8SO1125R000300120005-6Declassified and Approved For Release 2012/05/10 CiA-RDPB8EO1125R000300 1200056 Study of the morphological, functional and biochemical indicators, which ley sn eagential role in the formation of reactions of the organten, aie Cloted various Meordera in the experinental sniinals As is known, the energy of millimeter vaves, because of its weak penetrating ability, ic’abeoroea primarily and mainly by the skin. Our Investigations hhave shown that in tho akin of irradiated animals deformation of the receptor apparatus and well-expressed changes of a reactive character wore observed, In the ekin layer properly speaking appeared bunches of nerve fibere with hypertrophy of « portion of the fiber and sections with dengelint zation, In the dermis, anong the collagen titers were sual trunks of various thickne the neurel conductors of which vere fragnented in separate cases, and pheno- # nena of denyelintzation were observed in the surface Sayers, As the results of hstonorphological analysis showed, in the functionally active structures of tissue of the myocardiut, iiver, kidneys and spleen Gisordere of the henodynanice were cetabliched, vith dieruption of the per= Beability of the vesicular mexbranes, the appearance of tleronecrosee ant fubsoavent tiame dystrophy, Moreover, qualitative and quantitetive shifts were revealed in the erythrocytic and louxoaytic couposition of the blood of Lrredisted anise), Indleating suppression of the hemopoietic dunctién of the bone marsow and yaphatic system. Noted in the composition of the rea. lod was eosinophilia, neutrophilia and lymphopenia, and lowering of the heso- Blobin level and’ reduction of the number of erythrocytes were cbeerved, whieh was determined to a considerable degree ty the retention of erythrocytes in the bone sarrov. In the latter cecurred en increase of the muaber of erythro- Dlactie cells end decroase of cells of the leuksblastie series. Under the effect of miilineter aves of low intensity the degree of aftection depends on the general condition of the organies and evideneiy io not. se grest, a the observed disorders are in the main reversible. A characteristic feature of the biologica) effect of zadlowaves vas changes fof the state of various esctione of the centrel and vegetative nervous ate Yona which Involve directly or indirectly Ascrdere of the principal fnetions of tho organtans (i, I, Yakovlev, 1973). An 9 rerult of investigations conducted by us on fanle trrediated with milis- never waves, disorders of conitioned reflex activity have bean established: ‘weakening of the ctimlatory process, reduction of the size of the latent period in reoponse to different conditioned atiaaill (light, noise or pein) and * Adeinntbition of atfferentiation reactions. Disorders of the etimlatory and inhih'tory processes aisplayed in anials during the repeated effects of milit- meter radiovaves can he considered suppression of the fnetion of the central nervous systen, although the dovelopes inkinieson can be Linked with protectiver Coapensatory reuction of the orgaien in reepase to irraidetions Ln the Diced plema of Irradiated aninals the content of 17-ayeorticosterotda in unsed (22.6h 4 2.18 nig per 100 21 of plasma of the trredigted end 14.98 "2.01 akg of the wnirrediatea. Along with thet, in the edrenel cortex of COVERIBRENT USE ONLYDeclassified and Approved For Release 2012/05/10 : CiA-RDP88BO1126R000300120005-6 vaun Lrembbaten) by mk Utaeter wives te agiontbe weil tect Se rae ange eetabiched in tee vontent of £f-oee du the Hoo ‘he fonctsontygeorbie acid in the alrenal cortex of irradiated animals in- plocna wna Chfhuence of aillineter rediovaves on the central componeats of Gicote Ue alomechypopisie aysten -- the pirenel glands with involvenent of @ muber of hundral conponents the conducted investigations shoved thet in sninols subjected to the effect The conduetes fulowaves there vae a variation of the content. end ratio of oF ea retreat in the ploot the concentration increased, in the hypothslams gatecholemiine content ineraeaed and the norsireneline evel dropped, in the Tae arene ren tere vac e slight redistribution of eatecholenines, in t cer ores eras the adrenaline content doubled and the noradrénedine level seees Ey Tif in conparszon with thet in urkrradheted anttals, The adren- Sropped oy Mb ation in the adrenal. glands remained elevated vy 60 10 days shine concsnengletion cessed, he obteined results indicate vell-expressed seit ene petabolien of catecholamines under the influence of millsne ter saree Seth dn the hor#onsl and in the syupatiivtic componente of the sympath: saree eoenl aysten and elso reflect changes of the functional ectivity of its hormonal end mediator conponents. me main mass of the energy in tismes and orgens of animal organisms, os is The wold Piceased during the ologicel oxidation of orgerie subatances, in navy 36 "the greatcr part of it ie accumated in the form of macracrgs~ wie eeeGace of bromernetics, occtring meinly in the mitochondria with the are eesticipation of respiretory enzymes which accomplish the termine? Gireet Peijologieal onidation, are of universal Lmportence end assure Sh Fm chal activity of organa and tissues, the synthesis of proteins end functional, sets cormation of sone intermediate prolucts of exchenge, ete. ne conducted investigations shaved that the irradiation of animals ty mi21i- The con ee ped changes of the processca of orldative phosphorylation in never waves, Cfnove, heart and brain of the animals. The drradiation inhibited the Liver, Kiddcapidon rate ty the mitochondria of those drgans in the sctive ree eet tine aeate und shoved fom the rate of teepiradion uson exhaustion Phosphoryigtie She liver aid kidneys of irradiated eninals the intensity of Sean ertaton aeerenced by Ufy the values of the respiratory controls de Rae ar nd GDL respectively and the changes were ges expressed in the heart and vrata. te established disorders of the process of conjugate oxidstive phosphoryle- ae eee eettochonirig of irrediates animals testify to! suppression of Hor sn cohange und-can be a result of changes occurring in the electron trané- Fee eee tie cxpresced typothecks was confirded by the resulte of inves: aoe cae ce the otivity of ennmmes participating in the! processes of tissue Leet reo, in the itochondria of the Livers of irradiated aninala the Leer ec yaragenase activity Ancressed by 34 and the eytochromoxtdase secant vcreased ny ff. Those date testify to destruction of the cyto~ Shrowe chet.Declassitied and Approved For Release 2012/05/10 : CiA-RDP88BO1125R000300120005-6 CovesmENT USE ORLY 3 Very essential in tho eysten of enzymes of cell energy supply is the role fi of the ATPases regulating the processes of formetion and use of the enerey 3 of macroergs (V. P, Skulschev, 1969). The conducted investigations revealed f Sn the mitochondria of the livers of irradiated animale an increase of ATPase 3 activity ty 63 ee compared with eluilar indicators for the unirradiated. In ‘ thet ease in the Liver and spleen of animals irradiated many tines by milli eter waves there vas a decrease of the content of adenylnucleotiaes by 61 fand 6B respectively. Investigation of the influence of millimeter waves on the state of nucleic f exchange shoved that in the Liver, spleen, kidneys, lungs and heart there vas fa reduetion of the content, of mucleie acide and cufpression of he rate of Sugctiymidine in DNA and 1*Caurséine in RNA. Ina Bomparison of the reculte "(a of quantitetive determination of mucleic acids it was esteblished that the rate of inelusion of the predecessor in ENA ail its content 1p the orgine Pe changes leee than the DNA, The change of the miclete acide concentretion was 2 hore expressed in the Liver, spleen ant kidneys then in the heart and lunge. TTocether with reduction of the nuclele ackde content, the quantity of ackae soluble progicts in the liver ond spleen of irradiated entnale dnereasea by 35 ond 3H and the activity of ribonuclease and DNAase increased SOK. Under the influeree of radiowaves the protein spectrum of the blood seus changed (the elounin content decreased and the mnber of globulins increated, nich led to decrease of the value of the elbuiinegiobulin coaffictent) ond i the number of free amino acide decreaced by 22%, An indfeator of the reauced “ey level of protein synthesis in the irrajated animals also was the established Feguction of the rate of inclusion of “4C-nethionine in proteins of the Liver, Epleen, lysph nodes and thyme. The presented data testity to subetantiel chonges in the protein metabolism whieh occur under the Lafluence of multiple irvaltetion of animals ty millineter radiovaves. Evidently the reduction of fof the general energy level occurring in the arganimn under the influence of pialineter radlovaves liad an effect on the formation of macvoerge and caused ‘i 4 suppression of all Aunctions of the organtes, including suppression of aya= — thetie processes but especially of micleoprotein metabolisn, which 48 very’ enorey-consuning. ‘Te conducted experinontal Investigations vere coupered with observations of i the sente of health of 97 persons vorking with generators of the millineter range on the basis of systenatic contueting of biochemical analyses. The - A Sbesined daca confirmed the existence of an Snfluence of radlovaves ob the sy Stace of netabolie processes in the crganias, in pertiewar, changes of the ‘5 inaicotors of protein ana carbohydrate metabelian vere revealed and aistar- tf bances of the indSestore of ismuno-Uiologicel reactivity and of the blood iB tien were established. aa Me the conducted investigations inilcate high biological actévity and an a) Uifavoreble Angluence of millimeter radsowaves on the crgenion. The expres Ee hase of the Blologicel reactions increased with increase of the period Bf ite TaGmuion and depended on Snitviauel charectertsticn of the orgenten. Hf comeENT US eM ‘ lease 2012/05/10 ; CIA-RDPS8BD1125R000300120005-6<” Declassified and Approved For Jassified and Approved For Release 2012/08/10 : CIA-RDP88B01125R000300120005-6 praurosearte he punanchly, Yu Dey Serdyuk, A. Mey and Los', T. Ps Widyantye elektro busanerYa poley ratiochastot na cheloveks” [Tafloence of Blectromag a Melee of Raaio Frequencies on Man]. Kiev, 1975+ Gadchtrovey Me M, Ta: "Biologicheskiye effekty mikrovoln 4 ugroze, sor Saichshovssglogical Hefects,of Microwaves and Threat to the Health} Wareav, 2973. je smitechor, ¥. F, *Aiumlyateiya energis v Ketke” [Energy Aecumiation gene cel]. Noseow, 3968. bh, suuvote, A, G, In: "Viayantye SvCh-icluchenty ne organtzn cheloveks £ Setbotts A? frntiuence of Superhigh Frequency Redations on the Orgen- paveryan end Animale], Teningrad, 1970. inicheskiye espekty cblucheniya s¥on-atepazone” [Cline 5. Tyagin, BV Puss, Meee’ of Radletion of the supernigh Frequency Range). Leningrat, an. 6. cmiihtorin, B. N. Ins_ "Nedtko-bologicheskiye problesy S¥ch-tziveheny Paaneter ana: Biological Problene of Superhigh Frequency Raatations} enknered, 1966. 4. Yaxorieve, Me 1. "Piniolegicheskiye mekhaniony. deystyiya elektrenagndt- raereley"[Paysiologice: Mechanism of the Bftect of Electromagnetic Flelae]. Leningrad, 1973. COMMISH; Vracheoncye Tele 1977 aut 80: 1870