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Group 3
Pre-Laboratory Assignment
Give the factors affecting the enzyme activity and give the normal conditions for each factor to make
enzyme perform in its optimum activity
Factors:
1. Concentration of Enzyme
The higher the enzyme level the faster the reaction will proceed noting that the
substance is in excess.
2. Concentration of substrate
3. pH
Each enzyme has a pH value that it works at with their optimal pH level that stabilizes at
pH 7.0 – 8.0. Changes in pH may denature the enzyme.
4. Temperature
Each enzyme is extremely sensitive to thermal changes. Hence, there are certain
temperatures at which they are more likely active. 37o C to 40o C are their optimal
temperature or normal temperature. 40o C – 50o C will let the enzyme go under
denaturation.
5. Activators
To have their optimum activity some enzymes require certain inorganic metallic cations
like Mg2+, Mn2+, Zn2+ Ca2+, Co2+, Cu2+, etc. But rarely anions are needed for enzyme activity
like Chloride Ion (Cl-) for amylase.
6. Inhibitors
Inhibitors are chemicals that block or distort the active site. Inhibitors that occupy and
compete with substrate preventing it from binding to the enzyme are called Active site –
directed or competitive inhibitors. Inhibitors that attach to other parts of the enzymes
perchance distorting its shape are called non-active site – Directed or non-competitive
inhibitors.
Members:
References: