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Serial Dilution and

Microbial Count

It is difficult to know and imagine the size of


microorganisms, since it’s about 1/10
th, the size of a typical human cell, and
Abstract measured in the scale of one millionth of a
meter known as micrometer. However, it is
Microorganisms are those organisms that often important to know not only
are too small to see with the naked eye and what kinds of microorganism are present in
include things like bacteria, fungi, and a given or obtained sample but also to
viruses. However, the numbers of microbes know the number of microorganism in
in a given specimen are required not only sample which is difficult to count under the
to identify but also it is important to count naked eyes because due to their small size.
a particular aspect such as diary In addition, worldwide the numbers of
manufacturing, diseases research and microorganisms in a given sample are
study, and so on. required to know in certain aspects such as
dairy industries, water treatment, food
manufactures, and importantly in
investigation and study of diseases Due to
the above reasons, variety of methods had
been developed for quantitative
enumeration of microorganisms such as
direct microscopic counts, electronic cell
counters, chemical methods,
spectrophotometric analysis and serial
dilution agar ,plate analysis

A scientific paper submitted by Denise P. Lagata, BSN –IA, for partial fulfillment in the course, NPSO3L-
Microbilogy and Parasitology
Material and Methods

Saliva and

A scientific paper submitted by Denise P. Lagata, BSN –IA, for partial fulfillment in the course, NPSO3L-
Microbilogy and Parasitology