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Cryobiology
Volume 89, August 2019, Pages 104-108

Effect of different concentrations of L-carnitine in extender for


semen cryopreservation in sheep
Clara Vieira de Souza a, Felipe Zandonadi Brandão a, Juliana Dantas Rodrigues Santos a, Vivian Angélico Pereira
Alfradique a, 1, Vanessa Moreira Barbosa dos Santos a, Maria Clara da Cruz Morais a, Paulo Sergio Cerqueira Rangel b,
Andreza Amaral da Silva c, Joanna Maria Gonçalves Souza-Fabjan a
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https://doi.org/10.1016/j.cryobiol.2019.05.009 Get rights and content

Abstract
This study assessed the effect of L-carnitine (LC) in sheep semen extenders containing or not egg
yolk for cryopreservation in sheep. Two extenders (TRIS-egg yolk or the commercial optiXcell™
IMV medium) were used, totaling six groups: IMV – (0, 5 and 10​mM LC) and TRIS – (0, 5 and
10​mM LC). After the freezing-thawing process and throughout incubation at 38​°C for up to 3​h,
several parameters were evaluated: sperm kinetics, hypoosmotic, plasma membrane integrity,
capacitation status and lipid peroxidation level. The supplementation of either 5 or 10​mM LC
randomly affected some parameters and, overall, TRIS was superior (P​<​0.05) than IMV extender.
In the LC-groups, IMV had greater (P​<​0.05) oxidative stress than TRIS. In conclusion, although
LC affected isolated parameters, its supplementation in semen extender had no consistently
beneficial effect on freezing-thawing ram sperm.

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Keywords
Andrology; Antioxidant; Ovis aries; Semen preservation; Lipid peroxidation; CASA;
Hypoosmotic; Oxidative stress
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1 Present address: Faculdade de Medicina Veterinária, Av. Peter Henry Rolfs, s/n, Viçosa, MG, 36570–900, Brazil.

© 2019 Elsevier Inc. All rights reserved.

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