ASSOCIATION OF VITAMIN D RECEPTOR (VDR) mRNA EXPRESSION IN

NON-OBESE WOMEN WITH POLYCYCTIC OVARY SYNDROME

Muharam R1,2,3, Fuady M1, Harahap J1, Febri R1, Iffanolida P1, Mutia K1, Muna N1, Riayati C1, Ilma N1,
Wiweko B1,2,3, Andon H1,2,3
1 Human Reproductive Infertiliy and Family Planning Research Center, Indonesian Medical Education and Research Institute,Jakarta, Indonesia
2 Yasmin IVF Clinic, Dr. Ciptomangunkusumo General Hospital, Jakarta, Indonesia
3 Division of Reprodctive Endocrinology and Infertility Departement of Obstetric and Gynecology Faculty of Medicine Universitas Indonesia,
Dr. Ciptomangunkusumo General Hospital, Jakarta, Indonesia

BACKGROUND
PCOS is characterized by increased ovarian and adrenal androgen secretion, hyperandrogenic symptoms
such as hirsutism, acne and/or alopecia, menstrual irregularity, and polycystic ovaries. In addition, insulin
resistance and hyperinsulinemia are common features in PCOS women. Decreased Vitamin D concentra-
tion has been proven to be associated with PCOS. Vitamin D deficiency might be a causal factor in the
pathogenesis of metabolic syndrome in PCOS. Vitamin D plays important role in metabolic pathways
affected by PCOS and its levels have been related with the severity of this disorder’s phenotype. More-
over, vitamin D receptor (VDR) polymorphisms have been shown to have an association with insulin
resistance and some of the patterns presented by PCOS. Although most women with PCOS are overweight
and obese, some PCOS patients have normal or even low body weight. Thus, this study aimed to
determined the effect of VDR mRNA expression on lean PCOS.

METHODS
This was a cross-sectional study conducted on non-obese PCOS subjects and non-obese control subjects
between January and July 2018. The diagnosis of PCOS is established using Rotterdam 2003 criteria for
PCOS. mRNA expression of Vitamin D Receptor was measured using quantitative real time polymerase
chain reaction (qPCR). Correlations were evaluated with calculation of the Spearman coeffient.

RESULTS DISCUSSION
The 79 subjects enrolled in this study, 39 The incidence of PCOS was associated with lower vitamin
subjects (49.4%) belong to lean PCOS group. D levels of follicular fluid and decreased level of VDR gene
5,8
expression in granulosa cells, which was more dominant
in the PCOS patient with obesity.
2,1
This studies showed there was differences VDR gene ex-
pression between Lean PCOS and lean control. Lean PCOS
mean VDR mRNA Expression has lower level than control. There is no strong correlation
Lean Control between lean PCOS with VDR gene expressions
Lean PCOS There was a negative correlation between BMI increase
The gene expression data of VDR were and vitamin D levels in comparison with other patients.
significantly lower in Lean PCOS group
(mean ± S,2.13E-7 ± 5.18E-8) in comparison CONCLUSION
with lean control group (mean ± SD, 5.7E-8
± 5.18E-8). However, the correlation of VDR There is no significant association between PCOS and
mRNA expression on lean PCOS is not VDR mRNA expression especially in lean subjects.
statistically significant (p = 0.072). Further studies on larger sample population are required.
Correlation .
Age Testosterone BMI
VDR 0.04 0.05 -0.04 REFFERENCE
Using Spearman statistical test a negative 1. Aghadavod E, Mollaei H, Nouri M, Hamishehkar H. Evaluation of Relationship between Body Mass Index with Vitamin D Receptor Gene Expression and Vitamin D Levels o
f Follicular Fluid in Overweight Patients with Polycystic Ovary Syndrome. Int J Fertil Steril. 2017;11(2):105–111. doi:10.22074/ijfs.2017.4704

correlation was found between VDR expres-

2. Hm Krul-Poel, Yvonne & Snackey, Chantal & Louwers, Yvonne & Lips, Paul & Lambalk, Cornelis B & Laven, Joop & Simsek, Suat. (2013). The role of vitamin D in metabolic
disturbances in polycystic ovary syndrome (PCOS): a systematic review.. European journal of endocrinology / European Federation of Endocrine Societies. 169. 10.1530/E
JE-13-0617.

sion level and body mass index, the correla-

3. Wehr, E & Pilz, Stefan & Schweighofer, N & Giuliani, Albrecht & Kopera, Daisy & Pieber, Thomas & Obermayer-Pietsch, B. (2009). Association of hypovitaminsis D with me
tabolic disturbances in polycystic ovary syndrome. European journal of endocrinology / European Federation of Endocrine Societies. 161. 575-82. 10.1530/EJE-09-0432.
4. Seyyed Abootorabi, Maryam & Ayremlou, Parvin & Behroozi-Lak, Tahereh & Nourisaeidlou, Sakineh. (2017). The effect of vitamin D supplementation on insulin resistance
, visceral fat and adiponectin in vitamin D deficient women with polycystic ovary syndrome: a randomized placebo-controlled trial. Gynecological Endocrinology. 34. 1-6.

tion very weak and not significant. 5.

10.1080/09513590.2017.1418311.
Joham, Anju & Teede, Helena & Cassar, Samantha & Stepto, Nigel & J Strauss, Boyd & Harrison, Cheryce & Boyle, Jacqueline & De Courten, Barbora. (2015). Vitamin D in
polycystic ovary syndrome: Relationship to obesity and insulin resistance. Molecular nutrition & food research. 60. 10.1002/mnfr.201500259.