COMMISSION ON HIGHER EDUCATION

ANGELES UNIVERSITY FOUNDATION

College of Arts and Sciences

LABORATORY CAPABILITIES FOR BS BIOLOGY PROGRAM: CHED MEMO ORDER 49 and

AUF COMPLIANCE

CHED REQUIREMENTS AUF

1. Systematics
2. Genetics
Basic glassware and supplies for experiments
Equipment Required:
Microscope Compound microscopes (monocular,
Measuring tools binocular, trinocular)
Hot plate Vernier Calipers and meter stick
Refrigerator Refrigerator
Incubation oven Oven/Incubator
Fume hood Osterizer
Homogenizer Hot plate with magnetic stirrer
Equipment Desired: Autoclave
Multi hotplate stirrer Water bath
Laminar flow hood Oven sterilizer
Autoclave Centrifuge
Water bath shaker pH meter
Micro centrifuge Micropipette and micropipette tips
Electrophoresis apparatus w/ power supply Alcohol lamps
Spectrophotometer Blades
pH meter Forceps
Vacuum pump Cover slips
Micropipette Glass slides
Tran illuminator Cotton
Polaroid camera Colored cartolina
Microscope with mounted camera assembly Scissors
Chemicals Required: Graphing paper
Glacial acetic acid Laboratory glasswares
Carmine powder Centrifuge tube
Ethyl alcohol DNA extraction kits
Ether Electrophoresis
Colchicine Microcentrifuge
Supplies Required: UV Tans illuminator
Dissecting needles with curved ends Fumehood
Alcohol lamp Spectrophotometer
Blades Black and white buttons
Forceps Carmine powder
Cover slip Ethyl alcohol
Glass slides Culture bottles
Refluxing apparatus Starch
Culture bottles Chloroform
Cotton Agarose

CHED COE/COD in Biology

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 COMMISSION ON HIGHER EDUCATION
ANGELES UNIVERSITY FOUNDATION
College of Arts and Sciences

CHED REQUIREMENTS AUF

Absorbent paper Ethidium bromide
NA-RNA KIT Electrophoresis buffer and staining systems
Replication kit Blotting membrane
Gene action kit
Colored cartolina
Scissors
Seed box
Graphing paper
Calculator
Black and white buttons
Laboratory glasswares (beakers, flasks)
Chemicals Desired:
Tissue cultured media
Electrophoresis buffer and staining system for
isozyme analysis
Starch or polyacrylamide
Agarose
Chemical reagent for DNA Extraction
Chloroform
SS phenol
Sodium acetate
Restriction endonucleases
Ethidium bromide
Supplies Desired:
Blotting membrane
Non-radioactive labelling & detection kit
Films and developing supplies
Laboratory wares (pipette tips, disposable
centrifuge tubes, Saran wrap, plastic trays, etc.)

3. General Ecology
4. Microbiology
Basic glassware/materials for experiments:
Test tubes Test tubes (rimless) and test tubes with screw cap
petri dishes Erlenmeyer flasks
Erlenmeyer flask Petri dishes
serological pipettes Serological Pipette
inoculating loops Micropipettes
inoculating needles Inoculating needles
Required Equipment: Inoculating loops
Compound microscope one per student Compound microscope (monocular,
Balance, one unit per lab Binocular and trinocular)
Oven, one unit per lab Oven/Incubator
Stove, one unit per l;ab Electric stoves
CHED REQUIREMENTS AUF

CHED COE/COD in Biology

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 COMMISSION ON HIGHER EDUCATION
ANGELES UNIVERSITY FOUNDATION
College of Arts and Sciences

Refrigerator, one unit per lab Refrigerator

Bunsen burner or alcohol lamp Alcohol lamps
Autoclave Autoclave
Recommended Equipment: LCD television
Television and media player, one unit Various culture media
Overhead projector, one unit Biochemical test media
Required Chemicals and Supplies: Staining reagents
Culture Media
Biochemical Test Media
Staining reagents

5. Cell and Molecular Biology

Laboratory Equipment, Facilities, Chemicals
and Supplies:
Shaker incubators Biosafety cabinet
CO2 incubators Microscopes
Biosafety cabinet/s Centrifuge
Microscopes DNA extraction kits/reagents
Inverted Microscopes Centrifuge tubes (1.5ml, 15ml, 50ml)
Centrifuge Agarose
DNA extraction kits/reagents Electrophoresis
Plasmid Transformation kits/reagents (eg. pGLO Affinity Columns for chromatography
Plasmid kits from Biorad)
Centrifuge tubes (1.5ml, 15ml, 50ml)
Agarose
Electrophoresis Apparatus (for agarose gels and
Polyacrylamide gels)
Affinity Columns / Resins for Chromatography
Target-specific Primary Antibodies
Enzyme-conjugated Secondary Antibodies
Enzyme Substrate (e.g. DAB for HRP-linked
Secondary antibodies

6. Plant Physiology
Basic Glassware for experiments:
Burets Burets
250ml and 500ml graduated cylinders 10ml, 50ml, 100ml, and 500ml graduated
Test tubes and caps/stopper cylinder
Funnels Test tubes
50ml, 100ml, 200ml, 500ml, 1000ml beaker
1000ml brown bottles
Petri dishes
Minimum Laboratory Equipment/ Materials
(Required):

CHED COE/COD in Biology

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 COMMISSION ON HIGHER EDUCATION
ANGELES UNIVERSITY FOUNDATION
College of Arts and Sciences

1. General Biology I and II

Basic glassware and supplies for experiments Compound Microscopes (monocular)
compound microscopes Compound Microscopes (binocular)
histologic slides Compound Microscopes (trinocular)
balance Stereomicroscopes
oven Top loading balance
refrigerator Triple beam balance
stove refrigerator
dissecting/binocular microscopes Electric stoves
Optional: television and VCR, overhead projector histologic slides
LCD Television
LCD Projectors
2. Plant Morphoanatomy
3. Plant Physiology
Minimum Laboratory Equipment/ Materials Required Top loading balance
analytical balance triple beam balance
pH meter pH meter
UV-VIS spectrophotometer with cuvettes refrigerator
refrigerator magnetic stirrer/hot plate with magnetic bars
rough platform/pan balance mortar and pestle
magnetic stirrer/hot plate with magnetic bars water baths
mortar and pestle autoclave
water bath centrifuge
autoclave Oven
centrifuges (varying speeds/temperature ranges) micropipette
oven Osterizer
light meter Thermometers
cold cabinet Latex gloves
leaf area meter aspirators
growth chambers forceps
pipettors, variable volume capacities light meter
cork borer (set of variable sizes) fumehood (improvised)
blender UV-VIS spectrophotometer with cuvettes
thermometers cork borer
latex gloves
aspirators
forceps
Desired Equipment:
shaker
fume hood
clark type Probes (multi-probes)
Required Chemicals
petroleum ether acetone
Acetone (100%) chloroform
chloroform ethanol
resazurin dye magnesium chloride
potassium cyanate (KCN) petroleum jelly

CHED COE/COD in Biology

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 COMMISSION ON HIGHER EDUCATION
ANGELES UNIVERSITY FOUNDATION
College of Arts and Sciences

carbonyl cyanide chlorophenyl sodium chloride

hydrozone (CCCP) in di-methyl sulfide (DMSO) chlorine bleach
Petroleum jelly/parafin DMSO
sodium phosphate
potassium diphosphate
mannitol
potassium chloride
magnesium chloride
siccine acid
adenoside triphosphate
2,4-dinitrophenol
ethanol
sucrose
sodium chloride
chlorine bleach
indole-3-acetic acid
napthalene acetic acid
tween 20
lanolin
6-benzylaminopurin (BA)
trilodobenzoic acid
abscisic acid
cytokinin
gibberellic acid
4. Animal Physiology
Basic glassware and supplies for
experiments
kymograph 70006-00 Phipps and Bird Electric stoves
(Complete set: heart lever, muscle lever,
inductrium dissecting instruments
signal magnets, writing stylus (smoke/ink type) dissecting pans
student tambour, pneumograph, drum and
smoker) thermometers
Respirometer Stereomicroscopes
compound microscopes (monocular,
stove binocular
dissecting instruments and trinocular)
dissecting pans stage micrometer
turtle holder ocular micrometer
spot plate airconditioner
thermometers exhaust fans
stereozoom microscope tanks for frog specimens
compound microcope with ocular micrometer glass aquaria with aerators
stage micrometer fire extinguisher
electrophoresis kit and power supply First Aid Kit
air conditioner Basic glassware
electric fans
exhaust fans
fume hood

CHED COE/COD in Biology

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 COMMISSION ON HIGHER EDUCATION
ANGELES UNIVERSITY FOUNDATION
College of Arts and Sciences

holding pond for frog and turtle specimens

glass aquaria with aerators
fire extinguisher
First Aid Kit
5. Developmental Biology
Compound microscopes (monocular,
Compound microscopes and slides binocular
Optional: Television and VCR, overhead
projector trinocular
LCD television, OHP and LCD
projectors

6. Genetics
Required:
Compound microscopes (monocular,
Microscope binocular,
Measuring tools trinocular)
hot plate Vernier calipers and meter stick
refrigerator Refrigerator
Incubation oven Incubator/Oven
Fumehood Osterizer
Homogenizer Hotplate stirrer
Desired: autoclave
multi hotplate stirrer waterbath
laminal flowhood centrifuge
autoclave pH meter
waterbath shaker micropipette and pipette tips
microcentrifuge alcohol lamps
electrophoresis apparatus with power supply blades
spectrophotometer forceps
pH meter cover slips
vacuum pump glass slides
micropipette cotton
transilluminator colored cartolina
Polaroid camera scissors
microscope with mounted camera assembly graphing paper
Chemicals laboratory glassware
Required: centrifuge tube
glacial acetic acid DNA extraction kits
carmine powder electrophoresis
ethyl alcohol microcentrifuge
ether UV transilluminator
colchicine laminar flowhood
Supplies Fumehood
dissecting needles with curved ends spectrophotometer
alcohol lamp black and white buttons
blades carmine powder
forceps ethyl alcohol

CHED COE/COD in Biology

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 COMMISSION ON HIGHER EDUCATION
ANGELES UNIVERSITY FOUNDATION
College of Arts and Sciences

cover slips ether

glass slides culture bottles
refluxing apparatus laboratory glassware
culture bottles calculator
cotton graphing paper
absorbent paper
DNA-RNA kit
Replication kit
Gene action kit
colored cartolina
Scissors
seed box
graphing paper
calculator
black and white buttons
laboratory glassware
Desired:
Chemicals
tissue culture media starch
electrophoresis buffer and staining systems for chloroform
isozyme analysis agarose
starch or polyacrilamide ethidium bromide
agarose electrophoresis buffer and staining systems
chemical reagents for DNA extraction
chloroform
SS phenol
sodium acetate
restriction endonucleases
ethidium bromide
Supplies
blotting membrane
non-radioactive labelling and detection kit
films and developing supplies
laboratory wares (pipette tips, disposable
centrifuge tubes, Saran wrap, plastic trays, etc.)
7. General Ecology
Basic glassware for experiments
Required Various glasswares- beakers, flasks, test tubes
sling psychrometer alcohol thermometers
thermometer (soil and air) light meter
barometer triple beam balance
light meter top loading balance
wind meter salinometer/refractometer
oven/incubator oven/ incubator
weighing scale soil sieves
salinometer/refractometer plankton nets
sieves centrifuge
plankton net dissecting microscope

CHED COE/COD in Biology

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 COMMISSION ON HIGHER EDUCATION
ANGELES UNIVERSITY FOUNDATION
College of Arts and Sciences

dessicators Global Positioning System

centrifuge Secchi disk
refrigerator DO meter
dissecting microscope BOD bottles
transect line burettes
compass pipettes
spectrophotometer Flasks
furnace sediment sampler
quadrats (0.25m2 and 1m2) Schindler trap
vacuum pump spectrophotometer
secchi disc mechanical shaker
BOD bottles/burettes, pipetted, flask burettes
Desired:
DO meter (digital) and accessories O2 probe
and mechanical stirrer
sediment sampler
mechanical shaker
water sampler
Desired Chemicals and supplies
ascorbic acid acetone
Acetone (100%) potassium iodide
ammonium molybdate tetrahydrate sulfuric acid
antimony potassium tartrate potassium hydroxide
1-amino-2-naphtol-4 sulfuric acid manganous sulfate
EDTA starch
EBT-powder formalin
methyl orange sodium hydroxide
hydrochloric acid hydrochloric acid
sodium thiosulfate
sulfuric acid
manganous sulfate
ammonium hydroxide
potassium iodide
sodium iodide
potassium hydroxide
sodium hydroxide
phenolphthaline indicator
potassium dihydrogen phosphate
sulfanilamide
N-1 napthyl ehylene diamine
potassium nitrate
ammonium chloride
borax
sodium sulfite
spongy cadmium
cadmium sulfate
tartaric acid
starch

CHED COE/COD in Biology

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 COMMISSION ON HIGHER EDUCATION
ANGELES UNIVERSITY FOUNDATION
College of Arts and Sciences

formalin
silica
GF/C Filter Paper
8. Microbiology
Basic glassware/materials for experiments: test tubes, test tube with caps, Petri dishes
test tubes, Petri dishes, Erlenmeyer flask, Erlenmeyer flasks
serological pipettes pipettes
inoculating loops micropipettes
inoculating needles inoculating needles
Required: inoculating loops
Compound microscopes (monocular,
compound microscopes binocular,
balance trinocular)
oven oven/ incubator
incubator Incubator/Oven
stove Electric stoves
refrigerator Refrigerator
bunsen burner or alcohol lamp alcohol lamps
autoclave autoclave
Desired: LCD television
Television and VHS various culture media
overhead projector Biochemical test media
Required Chemicals and Supplies staining reagents
Culture media
Biochemical test media
staining reagents
9. Cell and Molecular Biology
Basic glassware and supplies for
experiments
beakers- various sizes Various glasswares- beakers, flasks, test tubes
flasks- various sizes pipettes
test tubes and racks micropipettes
pipettes- various sizes graduated cylinders
graduated cylinders- various sizes Petri dishes
Petri dishes autoclave
Basic Equipment top loading balance
autoclave centrifuge
balance- top loading and analytical electrophoresis
centrifuge (up to 20,000 rpm) plus accessories laminar flow
electrophoresis equipment and power supply hot plate and stirrer
Compound microscopes (monocular,
Hood (laminar flow, if possible) binocular,
hot plate/ stirrer trinocular)
microscope- compound pH meter
micropipettors (various volumes or adjustable volumes) Refrigerator
plus pipette tips LCD television
pH meter plus accessories LCd projector
refrigerator

CHED COE/COD in Biology

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 COMMISSION ON HIGHER EDUCATION
ANGELES UNIVERSITY FOUNDATION
College of Arts and Sciences

Optional equipment: television and VCR

overhead projector

CHED COE/COD in Biology

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