6AM-2PM SHIFT
- first thing in the morning get all the tubes, VITEK kits and
others from REF (Biochem)
Except Lactose broth only for water culture
- get all the plates BA, CA, MAC, GBA, BCA from ref
- put all agars and agars from canister to let afternoon shift to
know that they need to make
- clean the incubator, wipe with Lysol and put anabac
- clean the candle jar
- put plates (below/level 3) or the empty wave rack(to be
thrown the next day)
Wave rack-after cleaning or washing it with soap
- plates in level 1 are to be stacked up to 4 plates only.
- put the plates on the table and segregate with the request
forms
- put also fungal specimen on the table
- wound(subcultured) placed at back
- new wound specimens placed at front of rack
- put tubes in the waste container
- put paper on the basket (cover)
*Lysol solutions are already made, no need to dilute
For urine spx=
Big loop for mac, small loop for ba
Can use other loop for other specimens or cotton swab
First and second quadrant can use big loop and 1 loopful, for
other specimens.
TSB- wound with swab
- put in front if new wound specimen
- incubate directly
Amy’s transport medium- culture the spx in BA, CA, MAC
- swab the 1st quadrant; use loop for the rest of the
quadrants)
Pipette tips are to be autoclaved before using
Disinfect before and after UV and also before VITEK
In labeling paper for processing, please fill out all information
of the patient
2PM-10PM
When using BSC only one person is allowed to use at a time.
Do not get near BSC when UV light is turned on
MH plate are used when CULTURE AND SENSI FROM PLATES
mostly SUBCULTURED with names of px.
- streak vertical, horizontal and diagonal- 3 streaks only, not 4
or 5
-rim twice
- prepare red pencil, toothpick as forceps to get antibiotic
When processing, whoever logs it shall finish it.
Canister- get all plates inside to let others know its empty
Regarding antibiotics
- not all in one container
- placed by group or panel
- for enterics- include CEF
- if antibiotic for the panel is not placed in its container,
borrow from other containers
AFB
- when streaking AFB, streak it evenly and coiled to produce
distributed bacilli
- if MT is finished reading the slides, put in the box to be sent
to DOH
BODY FLUID
- place 3ml of body fluid inside yellow culture bottle
Chocolate agar slants is used for the short-term storage
of pathogens such as N.meningitidis, S.pneumoniae,
and H.influenzae for up to 1 week.

Beta hemolytic streptococci/ Group A S. pyogenes/

agalactiae
SOP’ s in media
Ampicillin
BA
Penicillin
1. THROAT SWAB
Cefepme
BA, MAC
Cefotaxime
1. URINE
Ceftriaxone
BA, CA, MAC
Chloramphenicol
1. Blood
2. Csf Clindamycin
3. Pleural
Daptomycin
4. Peritoneal
5. Synovial or joint fluid Erythromycin
BA, CA, MAC, THIOGLYCOLLATE BROTH Levofloxacin
1. WOUND Linezolid
2. ABSCESS
3. PUS Vancomycin
4. SCRAPINGS
5. EAR DISCHARGE
Haemophilus influenzae/parainfluenzae
6. EYE DISCHARGE 1. Amoxicillin clavulunate
2. Ampicillin
BA, MAC, BCA/BCA, Chocolate blood agar containing
3. Ampicillin sulbactam
bacitracin, GBA/GENTAMICIN BLOOD AGAR
4. Azithromycin
1. SPUTUM 5. Ceftriaxone
2. ENDOTRACHEAL ASPIRATE 6. Ciprofloxacin
7. Levofloxacin
3. NASOPHARYNGEAL SWAB
8. Meropenem
URETHRAL OR ENDOCERVICAL DISCHARGE 9. Tetracycline
10. Trimethoprim-sulfamethoxazole
1. CA
2. MTM/Modified Thayer-Martin Streptococcus pneumoniae

STOOL/RECTAL SWAB 1. Ceftriaxone non-meningitis/meningitis

2. Clindamycin
1. MAC 3. Erythromycin
2. SSA 4. Levofloxacin
3. TCBS/Thiosulfate-citrate-bile salts-sucrose agar 5. Meropenem
4. SMAC/MacConkey Agar with Sorbitol 6. Oxacillin
5. APW/ALKALINE PEPTONE WATER 7. Penicillin
8. Tetracycline
9. Trimethoprim-sulfamethoxazole
SF/STREPTOCOCCUS FAECALIS 10. Vancomycin
Staphylococcus 7. Cefotaxime
8. Cefoxitin
1. Cefoxitin 9. Ceftazidime
2. Ciprofloxacin 10. Ceftriaxone
3. Clindamycin 11. Ceftriaxone oral
4. Daptomycin 12. Ciprofloxacin
5. Erythromycin 13. Ertapenem
6. Linezolid 14. Gentamicin
7. Oxacillin 15. Imipenem
8. Penicillin 16. Meropenem
9. Rifampin 17. Piperacillin-tazobactam
10. Tetracycline 18. Trimethoprim-sulfamethoxazole
11. Trimethoprim-sulfamethoxazole 19. Fosfomycin
12. Vancomycin 20. Nitrofurantoin
21. Colistin
Pseudomonas aeruginosa
22. Classification:
1. Amikacin
23. Family Enterobacteriaceae
2. Aztreonam 24. Genus:
3. Cefepime 25. Escherichia
4. Ceftazidime * BOLD, true / Serratia
5. Ciprofloxacin 26. overt organisms Shigella
6. Colistin Hafnia
* ITALICIZED,
7. Imipenem 27. opportunistic Salmonella
8. Meropenem organisms Edwardsiella
28. Citrobacter
9. Gentamicin
Proteus
10. Piperacillin-tazobactam
29. Klebsiella
11. EDTA
Providencia
30. Enterobacter
Acinetobacter
Morganella
1. Amikacin 31. Erwinia
Yersinia
2. Ampicillin-sulbactam
32.
3. Cefepime
4. Ceftazidime
5. Ciprofloxacin
6. Colistin
7. Imipenem
8. Meropenem
9. Minocycline
10. Gentamicin
11. Piperacillin-tazobactam
12. Trimethoprim-sulfamethoxazole
13. Tetracycline

Enterics

1. Amikacin
2. Amoxicillin-clavulunate
3. Ampicillin
4. Aztreonam
5. Cefazolin
6. Cefepime