Chemistry Project Report

On Extraction and
Determination of
Caffeine in Tea Samples
Contains in Caffeine
 Contents
1. Introduction to the topic
2. Theory
3. Procedure
4. Calculations
5. Observations and Expected Errors
6. Results
 Caffeine in Tea Samples
Extraction and Determination
Chemistry Experiment

Most popular drinks in India are “Tea and

Coffee” As is the popularity of these drinks so
is the contribution of India in their production.
India has been the leading producer of tea.
Tea grows indeed, fertile & well drained soil. It
require warm and moist climate throughout the
year. Frequent showers, well distributed over
the year ensure continuous growth of tender
leaves. In India it grows mainly in low hills of
Assam, Hills of Darjeeling and Jalpaiguri,
Northern West Bengal and the hill of Nilgiri
etc.

Tea also causes many harmful diseases such as

Diabetes, problems in nervous system, decrease
in ADN secretion which causes increase in wine
secretion and it has Nicotine which is a
Stimulant.
Theory and Chemistry Experiment

Use difference in the solubility of caffeine and

tannic acid in water and chloroform to separate them
them from tea leaves.
Caffeine is much more soluble in chloroform than in
water. But tannic acid is extensively soluble in water
as well as chloroform.
Extraction of Water Soluble Components from
Tea Sample - Step-I

1. Take about 100ml of distilled water in 250ml

conical flask.
2. Add 5gm of tea and boil it for 4 to 5 minute.
Then cool it and filter.
3. Repeat this process again with 100ml of distilled
water and residual tea leave.
4. The filtrate in each case should be collected.
5. Now the wet tea samples may be dried in an over
and weighted.
Removal of Tannic Acid from Filtrate - Step-II

1. The filtrate obtained in the first step is now

boiled with 2-3 grm. calcium carbonate to get
tannic acid, present in tea extract, frecipitated.
On leaving it for ten minutes ppt. is obtained.
2. Filter the solution without disturbing the ppt
carefully. The ppt should be transferred to
filter paper only after all the filtrate is
removed.
3. Tannic acid has been extracted in the form of
calcium ternate which on treatment with
hydrochloric acid gives tannic acid.
4. The filtrate is now free from tannic acid and can
be used to extract caffeine.
Extraction of Caffeine from Tannic Acid Free
Filtrate - Step-III

1. Take tannic acid filtrate obtained in step 2(ii)

and add 100 ml of chloroform in it in a separating
funnel and allow it to stand for 10 minutes.
2. Again the same procedure as done in (i)
3. Mix if with the previous one.
4. From this chloroform extract, chloroform may
be distilled or evaporated.

The pale green residue obtained is crude caffeine.

Repeat this experiment with other brands of tea and
record your observation in a tabular form.

 Weight of tea used = W1 grm.

 Weight of Caffeine = W2 grm.
Calculation :
Percentage of solute caffeine = W1 / W2 x 100

Calculations of Double Diamond

 W1 = 6 gm
 W2 = 0.7 gm

Percentage of caffeine = 0.7 / 6 x 100 = 11.6%

Calculations of Lipton Tiger

 W1 = 6 gm
 W2 = 0.8 gm

Percentage of caffeine = 13.3%

Calculations of Taj Mahal

1. W1 = 6 gm
2. W2 = 0.7 gm
Percentage of caffeine = 11.6%

Calculations of Red Label

 W1 = 6 gm
 W2 = 0.72 gm

Percentage of caffeine = 12%

Observation Table
Sr. No. Name of Brand W1 W2 %age of
caffeine
1. Double Diamond 6gm 0.7 11.6
gm
2. Lipton Tiger 6gm 0.8 13.3
gm
3. Taj Mahal 6gm 0.7 11.6
gm
4. Red Label 6gm 0.72 12.0
gm
Experiment Results:

The tea sample of Double Diamond contain

highest amount of caffeine and hence :-

1. Gives better taste than others.

2. Is more harmful for our health.
Precautions and Expected Errors :
1. Chloroform should not be smelt directly for
a long time as it it self is toxic.
2. The measurement of weight of tea leaves
and all extract should be precise.
3. The measurement of weight of tea sample
and all extract should be precise.
4. The water used should be distilled.
5. Apparatus should be clean and dry.
6. No water should be added to any extract in
between the experiment as it may get
errors in calculation.
7. No prolonged heating should be done as
water may evaporate in large quantity along
with same volatile solutes.
 Bibliography

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