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Gregor P.

Jose
Postdoctoral Fellow, Department of Biology, IISER Pune, Pune- 411008, India
Email: gregor.jos@gmail.com, gregor@iiserpune.ac.in │ Phone: +91 9836256709

Academic Qualifications:
 PhD, Dept. of Biological Sciences, IISER Kolkata, West Bengal, India 2008-2014
 M.Sc., Biochemistry, Bharathiar University, Tamil Nadu, India 2004-2006
 B.Sc., Biochemistry, University of Calicut, Kerala, India 2001-2004
Research experience:
Post-Doctoral Fellow, Department of Biology, IISER Pune, India 2015-Present
PLiMAP Technology: Peripherally-associated membrane proteins (MAPs) function in a variety of cellular
pathways ranging from cell signaling, host immunity, host-pathogen interaction and vesicular transport.
Identification of MAPs from a complex mix of proteins and functional characterization necessitate
methods that report on binding to membranes of a defined lipid composition. We have developed a
photoactivatable fluorescent phospholipid as a generic reporter of peripheral protein-membrane
interactions. When incorporated into liposomes and exposed to UV, proteins bound to liposomes
become crosslinked with the fluorescent lipid and can be readily detected and quantitated by in-gel
fluorescence analysis. This modification obviates the requirement for high-speed centrifugation
common to most assays that detect liposome-bound proteins. We refer to this assay as Proximity-based
Labeling of Membrane-Associated Proteins (PLiMAP). We are extending this methodology to enrich
membrane associated proteins and identify novel membrane remodeling proteins.

PhD Scholar, Department of Biological Sciences, IISER Kolkata, India 2008-2014


Thesis title: “Studies on the effect of copper nanoparticles and copper complex on DNA molecules,
bacteria and cancer cells: implications in antibacterial and anticancer therapy.”
Metal nanoparticles by virtue of its higher surface to volume ratio and Surface Plasmon Resonance (SPR)
exhibits contrasting physical properties to bulk metal. We have identified a novel in vitro DNA
degradation potential of copper nanoparticles (CuNPs) mediated by singlet oxygen, and its ability to
induce apoptosis in cancer cell lines. CuNPs also showed a superior bactericidal activity over ionic
copper by inducing in vivo nucleic acid degradation, reactive oxygen species generation, and higher
copper transport into the bacterial cells.

Industrial experience:
Scientist (R& D), Biocon Limited, Bangalore, India 2006-2008
Contributed to the development of Insulin Glargine (BASALOGTM) from laboratory to industrial scale,
which is the first long acting insulin developed and marketed by Biocon Limited.
Publications:
 Jose, G. P., Gopan, S., Bhattacharyya, S., Pucadyil, T. J., A facile, sensitive and quantitative
membrane-binding assay for proteins (Manuscript under revision in Traffic)
 Nisal, R., Jose, G.P., Shanbhag, C., and Kalia, J., (2018) Rapid and reversible hydrazone
bioconjugation in cells without the use of extraneous catalysts, Org. Biomol. Chem. 16, 4304-
4310.
 Bhattacharyya, S., Sarkar, A., Dey, S. K., Jose, G. P., Mukherjee, A., and Sengupta, T. K. (2013)
Copper(II) complex of methionine conjugated bis-pyrazole based ligand promotes dual pathway
for DNA cleavage, Dalton Trans. 42, 11709-11719.
 Hatai, J., Pal, S., Jose, G. P., and Bandyopadhyay, S. (2012) Histidine Based Fluorescence Sensor
Detects Hg2+ in Solution, Paper Strips, and in Cells, Inorg. Chem. 51, 10129-10135.
 Hatai, J., Pal, S., Jose, G. P., Sengupta, T., and Bandyopadhyay, S. (2012) A single molecule multi
analyte chemosensor differentiates among Zn2+, Pb2+ and Hg2+: modulation of selectivity by
tuning of solvents, RSC Adv. 2, 7033-7036.
 Soni, J., Jose, G. P., Ghosh, S., Pradhan, A., Sengupta, T. K., Panigrahi, P. K., and Ghosh, N.
(2011) Probing tissue multifractality using wavelet based multifractal detrended fluctuation
analysis: Applications in precancer detection, In Biomedical Engineering and Informatics
(BMEI), 2011 4th International Conference on, pp 448-452, IEEE
 Jose, G. P., Santra, S., Mandal, S. K., and Sengupta, T. K. (2011) Singlet oxygen-mediated DNA
degradation by copper nanoparticles: potential towards cytotoxic effect on cancer cells, J.
Nanobiotechnology 9, 9.

Patents:
 Jose, G. P., and Pucadyil, T. J., (2019) A novel bifunctional lipid probe for Proximity Labelling
based Identification of Membrane-Associated Proteins, Application no: 201921022074
(Provisionally patented in India)

Technical Expertise:
 Synthesis of UV-activatable and clickable phospholipids
 Metabolic labelling of phospholipids with clickable analogs and click reaction-based pull-down of
lipid-binding proteins
 Mass spectrometry: QTRAP 4500 MS/MS (SCIEX) for lipidomics and Triple TOF 6600 (SCIEX) for
proteomics
 Cell Biology: Cell culture, confocal microscopy, FACS, lentiviral transduction
 Molecular Biology: Gene cloning, protein expression and purification, in vitro transcription
 Software skills: MaxQuant, LipidView, Image J, Graphpad Prism, Adobe Illustrator

Awards and recognitions:


 Qualified for DBT-CTEP travel grant for attending Biomembrane days 2019 in Berlin, Germany
 National postdoctoral fellowship from Science and Engineering Research Board (SERB) India,
2017
 D. P. Burma Best poster award, 78th Annual General Body Meeting of The Society of Biological
Chemists (India), 2009
 Secured Junior Research Fellowship (JRF) in Life Sciences for pursuing PhD, awarded by Council
of Scientific and Industrial Research (CSIR), Government of India, 2008
 Qualified in Graduate Aptitude Test in Engineering (GATE) in Life Sciences, 2006
 Secured 3rd Rank in B.Sc. Biochemistry course at Calicut University, Kerala, India, 2004

Invited talks:
 Presented an invited talk at IISER Kolkata, West Bengal, India entitled “Proximity Labeling-based
Identification of Membrane-associated Proteins” on 11.09.2019.

Poster presentations (selected):


 “Comparative studies on the antibacterial action of ionic and nano copper” EMBO-India
Bioscience YSN meet (2013) Bangalore, India
 “Antibacterial and anticancer activity of copper nanoparticles” 78th SBC(I) annual meeting,
(2009) Bangalore, India
Conference organization:
 Organizing member for the “National Post Doc Symposium 2019” to be held at IISER Pune on
Dec 11-13, 2019 (https://sites.google.com/view/npds2019-pune/home?authuser=0)
 Organizing member for the “Biophysics Paschim conference” held at IISER Pune, 4th Nov 2017
(https://sites.google.com/view/biophysicspaschimnovember2017/home)

Professional memberships:
 Life member of The Society of Biological Chemists (India)
 Life member of Proteomics Society, India
 Member of International Chemical Biology Society

REFEREES

Dr. Thomas J. Pucadyil (PhD) - Postdoc supervisor


Associate Professor,
Department of Biology,
Indian Institute of Science Education and Research (IISER) Pune
Pashan, Pune, Maharashtra - 411008, India
Email: pucadyill@iiserpne.ac.in

Dr. Tapas K. Sengupta (PhD) - PhD supervisor


Professor,
Department of Biological Sciences,
Indian Institute of Science Education and Research (IISER) Kolkata
Mohanpur, Nadia, West Bengal - 741246, India
Email: senguptk@iiserkol.ac.in

Dr. Subhajit Bandyopadhyay (PhD)


Professor,
Department of Chemical Sciences,
Indian Institute of Science Education and Research (IISER) Kolkata
Mohanpur, Nadia, West Bengal - 741246, India
Email: sb1@iiserkol.ac.in