Report

on
Agro-Industrial Attachment
(AGR-407)
SUBMITTED IN PARTIAL FULFILLMENT OF THE
REQUIREMENT FOR THE DEGREE OF
BACHELOR OF SCIENCE (HONS.) IN AGRICULTURE

Submitted by
Name: GANNEBOYINA GOPALAKRISHNA
Registration Number: 11610993

Under supervison of

Dr. Subash kumar jawla

School of Agriculture, Lovely Professional University,

Phagwara-144411, Punjab, India

2019

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 Declaration

I hereby declare that the report presented herein is an authentic record of my work carried out under Agro-
Industrial Attachment at NRI AGRITECH PRIVATE LIMITTED as requirement for the degree of
Bachelor of Science (Hons.) in Agriculture and no part of this has been copied from any other sources.

GANNEBOYINA GOPALAKRISHNA

(Registration No.11610993)

For Agro-Industry

Official Name: ALAPATI RAJENDRA PRASAD

Designation: MANAGING DIRECTOR.

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 Acknowledgement

I would like to express deepest and heartful thanks to Alapati Rajendra prasad garu, managing

director for giving opportunity to complete the agro industrial attachment at NRI AGRITECH

PRIVATE LIMITTED. My sincere thanks to k. sambha siva rao, the general manager who created

the opportunity for undergone internship in research and development and seed processing unit.

My greatful thanks to peta gopi sir, the pulse breeder helped so much, and tought many new things

regarding the plant breeding techniques and crossing methods in different crops. I came to know so

many new things and really thankful to them.

Iam very thankful to other breeders sivarao sir, sivanjineyulu sir, sambha sivarao sir, Bramham

sir, surya sir and workers for interacting positively in every time.

We would like to express our gratitude to school of agriculture, lovely professional university

Punjab. Last but not least I would like to express special thanks to the Subhas kumar jawla sir the

supervisior for giving this opportunity to complete my agro industrial attachment.

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 Table of content

Chapter No. Contents Page number

1 Introduction 8-9

2 Nature of attachment 1 10-13

3 Process outline 14-20

4 Observations 21-23

5 Certificate 24

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 Introduction

NRI AGRITECH PRIVATE LIMITTED:

To face the challenges of the after effects of Green revolution, Professionals migrated from rural
India and settled in USA and highly qualified Agricultural Scientists in India under the leadership of
Dr. Alapati Satyanarayana a renowned Plant Breeder promoted NRI Agritech PVT. LTD, in the
year 2008, with a collective will and social responsibility to improve the lives of farmers through
improved seeds and education. NRI Agritech Pvt Ltd aims to make agriculture profitable by
innovating high yielding hybrids / varieties of various crops utilizing cutting edge technologies and
by creating awareness on sustainable agricultural practices. We consider education as a primary
resource in agriculture development and is as important as seed.

Nri Agritech Private Limited is a Private incorporated on 28 May 2008. It is classified as Non-govt
company and is registered at Registrar of Companies, Vijayawada. Its authorized share capital is Rs.
30,000,000 and its paid up capital is Rs. 26,400,000. It is inolved in Agricultural Activities like
developing new varities and distributing the quality seeds to the farmers.

Infrastructure

• Seed is the critical input for agriculture. High reputation of the firm is through quality seed
only.
• The company is growing swiftly maintaining the International standards of its products
(varieties and hybrids).
• Professionally managed with reputed Technocrats and qualified staff working in all
divisions.
• Located in the agriculturally prosperous city Guntur in the Eastern region of Andhra Pradesh
state.
• NRI Agritech has established a model processing plant for processing to quality seed, well
secured storage structures for processing and storage of quality seed in all seasons, Poly
house, net house for hybrid seed production, seedling production, and cultivation of high
value crops under protective agriculture.
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 • NRI AGRITECH also impart training to young entrepreneuring farmers on these aspects and
encourage rural employment.
Seminars and Symposia organized:
• Organized a National Seminar on developing Vegetable varieties for round the year cultivation
on 11 th January 2014.
• Launched “Grow your own Vegetables for health and happiness” on 23-02-2011 organized, in
collaboration with the department of agriculture.
• an exposure visits to the field of NRI to all officers of department of agriculture of Guntur
district along with 500 farmers at R & D form Godavalli on 27-11-2010.
•

Reverse Breeding-a new concept to develop varieties from hybrids. Lead paper presented at the
‘National symposium on abiotic and biotic stress management in vegetable crops’ at Varanasi on 12-
14th April, 2013.

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 Nature of Attachment

As industrial attachment was carried in RESEARCH&DEVELOPMENT and SEED PROCESSING

UNIT IN NRI AGRITECH PVT.LTD.

NRI AGRI TECH PVT.LTD.
RESEARCH&DEVELOPMENT

SEED PRODUCTION UNIT

SEED PROCESSING UNIT

SEED MARKETING UNIT

RESEARCH AND DEVELOPMENT:

“R&D IS HEART OF SEED INDUSTRY”.

➢ Research and Development of new hybrids and verities is the back bone for sustenance of
any seed company.
➢ Strong Research and Development requires intelligent leadership.

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 ➢ NRI AGRITECH Research and Development wing has been headed by the renowned
Geneticist and Plant Breeder, Dr. Alapati Satyanarayana, popular as Dr. Alapati among the
scientific community.
➢ NRI has established a R&D facility in 2008, with a strong base under the able leadership of
Dr. Alapati and his dedicated team of scientists.
➢ Pioneered in development of 60 successful and popular Varieties / Hybrids in a variety of
crops, in a short span of 7 Years of its inception, by Conventional as well as modern Plant
breeding techniques.
➢ The is company is involved in innovative plant breeding and genetics research to develop
new hybrids and verities of several field crops for better yields, quality, improved resistance
to biotic and abiotic stresses with new plant type characters, Ideal plants types…etc
➢ NRI AGRITECH worked on a variety of crops like Rice, Chilli, Maize, Cotton, Red gram,
Black gram, Green gram, Chick pea, Soybean, Castor, Sesamum, Mustard, Dolichos Beans,
Okra, Vegetable cowpea, Cluster bean, Tomato, Cucurbits.
➢ Reverse Breeding-a new concept to develop varieties from hybrids.
➢ Conducted the survey on inheritance of plant habit and photo-insensitivity in garden bean
Dolichos lablab var. typicus (syn.Lablab purpurians var. typicus). Paper presented at the
National Seminar on ‘Developing og vegetable varieties for round the year cultivation’ at
Guntur.
➢ Developed Bush Dolichos for round the year cultivation with vegetables pod quality like
kanupuchikkudu through Back cross method of Breeding.

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SEED PROCESSING:

The company has established a modern seed processing plant and other state of art facilities to
achieve high standards of physical purity for which the company is accredited with ISO 9001-
2008 certificate.

The processing plant is ISO 9001- 2008 certified and use State of art Technology and
Equipment at all stages of processing to ensure high quality (Physical and Genetic purity and
viability) and maintain the seed with optimum moisture level during storage.

Seed processing include drying, cleaning and sorting (grading and gravity separators) of seed.
Treat the seed with insecticides and fungicides. At the same time the seed is subject to physical
and genetic purity, germination tests and moisture content. After receiving satisfactory pass
results on various tests, the lots of seed are permitted to pack in suitable containers and sent to
disturbers for marketing.

Seed sampling before and after processing for

genetic as well as physical quality analysis.

seed cleaning, grading

quality control

seed treatment

seed packing

Quality Control

➢ Grow out tests for genetic purity

➢ Moisture content & to maintain optimum moisture % as per the seed standards

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 ➢ Germination and vigour tests
➢ Seed borne diseases
➢ Physical purity through seed cleaning grading, sorting
➢ Seed treatment with appropriate insecticide and fungicide
➢ The company is well equipped with laboratory facilities for testing viability,
germination, vigour, seed borne diseases etc., before releasing high quality seed for

SEED SAMPLING

SEED SOWING

REMOVAL OF OFFTYPES SEEDS TAGGING IN COTTON

MANUALLY

TAGGING IN
CHILLI
SEEDLINGS
IN NURSERY
AREA

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 Process outline

NRI AGRITECH VARIETIES

CROP VARIETIES

PADDY DELTA, NRI MASOORI, BEEJA, BHEEMA

MAIZE SIVA AND SONA

REDGRAM RAJA, ARJUN, SURYA, ESWARI, KIRAN

BLACKGRAM SRI, MARUTHI, NANDI.

GREENGRAM AMULYA AND SHAKTI

CHILLIES SAI, JAYA, NRICH-4,8,11,12,14,15,16,17.

RAVITEJA, SIVANI AND SRUSTI

OKRA LAKSHMI AND ASOH-1,2

DOLICHOS BEANS PAVAN (NRI BDL 1), GANESH (NRI BDL 2)

TOMATO BALAJI (NRI T001), PADMA (NRI T002)

SNAPMELON KANAKA (NRI SM001)

RIDGE GOURD RAGAHAVA (NRI RG001)

VEGETABLE COWPEA ANJALI (NRI VC001)

MARIGOLD NRI BANTI (NRI MG001)

CHICK PEA SARIKA (NRI KG 001), PRIYA (NRI BG 001)

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 DEVELOPMENT OF DOLICHOS VARIETIES

Ganesh and pavan are two varieties which are developed from local chikkudu and yebbala vare by

back cross method at nri agritech R&D. NIDUMUKKALA, GUNTUR.

LOCAL CHIKKUDU YEBBALA VARE

S.N.: Dolichos lablab var. perpurence S.N.: Dolichos lablab var. typicus

Local variety (recessive parent) Karnataka variety(dominant parent)

Photo sensitive variety Photo insensitive variety

Good pod quality Pod is non ediable

Tailing nature (stem), creeper Bushy type and standing (stem)

Main stem bearing Normal stem bearing

➢ This colour text indicates the desirable charecteristics which have to be combined in
new developing varieties.
Brief summery about developing dolichos varieties:
➢ Ganesh and pavan are the new developed varieties from the above existing varieties
by back cross method.
➢ Local chikkudu is used as recessive female parent and Karnataka variety is used as
dominant male parent.
➢ The desirable charecters are: photo insensitive in nature
Good pod quality
Bushy type stem
Main stem bearing.
➢ After crossing the varieties obtained f1 with charecters of photo insensitive, bushy
type and local pod. Then f1 was selfed, f2 was obtained.
➢ F2 were 3:1 ratio of bushy: creeper respectively. F2 was backcrossed with recessive
female parent.

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 PROCEDURE WAS FOLLOWED DURING DEVELOPMENY OF DOLICHOS VARITIES

Dolichos Dolichos
lablab var. lablab var.
typicus.(DD) typicus.(dd).
F1
(Dd)
photo insensitive
bushy type
local pod

F2 progeny
selfing
3:1

F3 progeny Back crossed with

9:3:3:1 female recessive parent

Selection and evaluation

F4 progeny/ bc1f1
was carried for desirable
progeny
charecters.

➢ In f3 generation 4 types of genotypes were obtained:

✓ Tailing, photo insensitive

✓ Tailing, photo sensitive
✓ Bushy, photo insensitive
✓ Bushy, photo sensitive.

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 ➢ In f4 generation the selection was carried and tested in replicated yield trail and released

varities as GANESH (RED SEEDED) AND PAVAN (WHITE SEEDED).

DEVELOPMENT OF BLACK GRAM VARIETIES

▪ Black gram is very important grain legume crop grown in andra Pradesh.In andra Pradesh
Guntur district ranks 1st for the production of black gram.
▪ The area and productivity of black gram declined in andra pradesh due to yellow mosaic
virus (YMV)disease.
▪ Development of YMV resistant varieties is prime importance for stabilizing the yield levels.
▪ The study was carried out at disease screening fields established at R&D farm, NRI
AGRITECH.2014.
▪ for development of YMV resistant varieties NRI AGRITECH used the varieties LBG(LAM
BLACK GRAM)402, LBG645 & LBG685 as female parents(susceptible varieties) and
PU(PANT URD)31 as a male parent (resistant varieties).

❖ YMV resistance is complementary gene action. In f2 generation the genes are segregated in
9:7 ratio(complementary gene action).
❖ In f3 generation 3 types of gene actions are observed i.e. true bred lines, resistsnce lines and
susceptible lines.

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 ❖ The susceptible lines are eliminated and roug out and resistant lines are selected and
evaluated for screening of developed varieties and conducted the replicated yield trail.
❖ After the replicated yield trail the developed varieties are named as SRI(LBG402 * PU31),
MARUTI(LBG685 * PU31) and NANDI(LBG645 *PU31).

Hybridization-
Collection, evaluation
recombinant breeding-
and utilization of germ Selection evaluation
product- evaluation for
plasm
varieties

Parental line
Testing of new hybrids Permission for
development and
& varieties in farmers marketing & release of
hybrid development-
fields new hybrid & varieties
evaluation for hybrids

Nucleus seed
SEED MARKETING
production

FIELD OBSEVATION ALONG WITH

CROSSING IN COWPEA
PULSE BREEDERS AND OFFICIALS.

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 F4 GENERATION OF BG&GG FOR YMV RESISTANCE SCREENIG
SEED PROCESSING:

Seed processing include drying, cleaning and sorting (grading and gravity separators) of seed. Treat
the seed with insecticides and fungicides. At the same time the seed is subject to physical and
genetic purity, germination tests and moisture content. After receiving satisfactory pass results on
various tests, the lots of seed are permitted to pack in suitable containers and sent to disturbers for
marketing.

SEED PROCUREMENT
FROM FARMERS AND
MOISTURE TESTING DRYING
NRI SEED
PRODUCTION UNIT.

GRADING WITH
GRAVITY SEPARATOR/
FINE CLEANING PRECLEANING
SPECIFIC GRAVITY
SEPARATOR

SEED PACKAGING
SEED TREAMENT SEED MARKETING
AND LABELLING

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 SEED PROCESSING PLANT

MACHINARY EXPLANATION

GODOWN

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 Observations

As industrial attachment was done in NRI AGRITECH PVT.LTD. it is seed industry which consists
of different units which are R&D, seed production, seed processing and marketing. observed many
things and learnt new techniqes in plant breeding.

RESEARCH AND DEVELOPMENT:

Nursery Management:

❖ Nursery preparation for different crops like tomato, brinjal, marigold, cabbage, chillies and
nursery beds were prepared separately for parental lines, f1 lines, test progeny, true bred
lines etc.
❖ All nursery beds are raised beds because the raised beds are most suitable for growth of
vegetable and flower crops.
❖ sowing of chilli, tomato, brinjal, marigold, cabbage seeds on prepared nursery beds.

Dolichos seed sowing for germination test:

❖ seed sampling was done (100 seeds are counted and prepared for sowing).
❖ Two types of seeds are sowned for testing germination in dolichos(pavan):
1. normal seed (without any chemical treatment)
2. treated seed (thiram +thiomethoxam)
❖ 62 lines are sowned with normal seeds and 62 lines are sowned with treated seeds, after 6-8
days observed normal seeds are germinated more.
➢ Male lines (r line) in maize was sowned for maintaining the hybrid seed production.

Chilli transplanting:

➢ Nursery was prepared for chillies and managed upto 14-16 days.
➢ Nursery was prepared for different lines like parental lines, tester lines, newly developed
varieties and tagged each and every line.
➢ Basal application of DAP in main field before transplanting the chillies and transplanting in
main field.

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Field observation:

➢ dolichos was sowned for germination test

➢ grow out test (GOT) in chilli
➢ Yellow vein mosaic virus resistant screening in okra crop
➢ Yellow mosaic virus resistant screening in black gram and green gram
➢ Selection of superior individuals plants in black gram (f4 generation)
➢ Crossing and pollination in cowpea and maize
BLACK GRAM GREEN GRAM

Semi lanceolate or lanceolate leaf ovate shaped leaves

Hilum is raised
Hilum is sunken
pale green
Dark green

➢ Emasculation in okra was done in evening time (4pm) and pollination was done next
morning (9:30 am).
➢ Tagging was carried in cotton field for easy identification of different lines.

Seed processing unit:

➢ seed sampling was done for examine the germination percentation, physical purity, viability
etc. in black gram and green gram.
➢ Observed many equipments/machinaries, their mechanisms and processing layout for seed
processing. CROSSING OKRA
TRANSPLANTING CHILLI

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SUGGESTIONS:

➢ Infrastructure is small and organization needs to be improved.

➢ Marketing structures are small
➢ Nri agritech concerning about pulses and vegetable crops.
➢ If it is better proper structural facilities would there.
➢ Seed production unit need improvement in structural facilities.
➢ Proper polyhose should be there for the cultivating the vegetable crops.
➢ Nri agritech not using the bio control agents for managing the pest and disease in reaserch
fields and following the conventional methods.
➢ Nri agritech appling the pedigree and back cross methods for developing the new varities so
the pedigree and backcross metods are time consuming methods.
➢ Proper laboratory should be there for research and development.
➢ Drip irrigation is not following in Nri agritech for irrigating the research fields.

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 Certificate

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