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EXPERIMENTAL DESIGN

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BIOLOGY
Osmosis and Diffusion

Name: Olga Damaskova


Candidate number: 000771-
Examination session: May 2012
Design an experiment studying diffusion and osmosis
Olga Damaskova
Candidate number 000771-
Date of elaboration: 31.9.2010
Design an experiment studying diffusion and osmosis
Olga Damaskova
Candidate number 000771-

Research question: What is the effect of light intensity on the rate of osmosis
in plant cell? Find out using the egg to represent the cell.

Hypothesis: Light affects the osmosis in plant because it regulates the stomata
opening. But the egg cell is considered to be an animal eukaryote, therefore it
has no stomata opening which have to be regulated. Question is whether the
light has an effect on animal cell and if yes, how much. Considering that light has
the effect on many processes in animal cells, for example it inhibits or stimulates
the hormone secretion and so I suppose that it will affect the osmotic rate, even
if in small amount.

Variables
Independent variable: Light intensity
Dependent variable: change in mass of egg (in %)
Constant variables: Concentration of the solutions, very similar egg (tissue
types, Molecular size, Surface area to volume ratio), temperature, initial volume
of a solution

Materials: vinegar, water, salt, 3 eggs, 2 boxes, balance, 3 measuring cylinders

Methods:
1. Place the eggs into the vinegar so the egg becomes soft (Calcium
carbonate is contained in the egg and makes it actually hard. When
vinegar, which is an acid (acetic acid), is added, a chemical reaction
which is responsible for the releasing of carbon dioxide takes place.
Due to this process carbon from the calcium carbonate is released
and thus the egg becomes soft and osmosis can take place).
2. Weigh the eggs
3. Prepare a hypertonic solution in each cylinder by salting it ( 2
spoons of salt in 100 ml of water )
4. Make sure that the volume in each cylinder is the same
5. Cut several holes into one of the boxes
Design an experiment studying diffusion and osmosis
Olga Damaskova
Candidate number 000771-
6. Place one cylinder into the box with holes, other without holes to
isolate it from light and the last place freely in the room so it has
no isolation from light.
7. Into each cylinder place the prepared egg
8. After some time weigh the eggs and calculate the percentage
change of its mass/ observe the change in ml by checking the
scale of measuring cylinder ( it might be helpful to check the
weighed values, however, not necessary)
9. Compare the 3 eggs, deduce the effect of light on osmosis

References

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