EXPERIMENT NO.

DISSOLVED OXYGEN LEVELS IN NATURAL

WATERS

Name: Adrian Niel B. Casabar Program / Year: CE-1

Course / Section: CM101L DOP: JUNE 16, 2019
Group Number: 6 DOS: JULY 23, 2019

Grade:
PDS: /40
Cover: /10
Objective: /10
Report Sheet: /10
E. S. Espiritu Discussion and
Professor Conclusion: /20
 EXPERIMENT NO.6

DISSOLVED OXYGEN LEVELS IN NATURAL

WATERS

Objectives:

1. Determine the amount of dissolve oxygen (DO) in the series of water sample.

2. Discuss why dissolved oxygen is such an important measure of water quality.

3. - Students should be able to perform the Winkler titration to test for Dissolved Oxygen. –

4. Explain the reasons for and circumstances associated with changes in dissolved oxygen

level.
Dissolved Oxygen Levels in Natural Waters Report Sheet

A. Sample Analysis

Sample 1 Sample 2

1. Sample volume (mL) 200 mL 200 mL

2. Buret reading, initial (mL) 0 mL 0 mL

3. Buret reading, final (mL) 8.1 mL 9.5 mL

4. Volume Na2 S2 O3 dispensed (mL) 8.1 mL 9.5 mL

5. Molar concentration of Na2 S2 O3 0.02517 M

(mol/L)

6. Moles of Na2 S2 O3 dispensed 0.000204 mol 0.000239 mol

7. Moles of I3− reduced by S2 O2−

3 – (mol) 0.000102 mol 0.000120

8. Moles of O2 (mol) 0.0000510 mol 0.000590 mol

9. Mass of O2 (mg) 1.63 mg 1.91 mg

10.Dissolved oxygen, ppm O2 (mg/L) 8.16 mg/L 9.56 mg/L

11 Average dissolved oxygen. ppm O 8.86 mg/ L

12. Standard deviation 0.49

13. Relative standard deviation 5.59%

Calculation Zone:

Volume Na2 S2 O3 dispensed (mL): 8.1 mL – 0 mL = 8.1 mL

8.1𝑚𝐿
Moles of Na2 S2 O3 dispensed: 0.02517 𝑀 × = 0.000204 mol
1000 𝑚𝐿

1
Moles of I3− reduced by S2 O2−
3 – (mol): 0.000204 mol × = 0.000102 mol
2

1
Moles of O2 (mol): 0.000204 mol × = 0.000051 mol
4

32 𝑔 1000 𝑚𝑔
Mass of O2 (mg): 0.000051 mol × × = 1.63 mg
1 𝑚𝑜𝑙 1𝑔

𝑚𝑔𝑂2 1.63 𝑚𝑔
Dissolved oxygen, ppm O2 (mg/L): 𝑑𝑖𝑠𝑠𝑜𝑙𝑣𝑒𝑑 𝑂2 = = 1𝐿 = 8.16 mg/L
𝐿 𝑠𝑎𝑚𝑝𝑙𝑒 200 𝑚𝐿 ×
1000 𝑚𝐿

8.16+9.56 𝑚𝑔
Average dissolved oxygen. ppm O: = 8.56
2 𝐿
0.999
Standard deviation: = 0.49
2
0.49
Relative standard deviation: 8.86 𝑥100 = 5.59%
Results and Discussion

The 200mL of water sample was carefully collected so that no air bubbles remain trapped in the

plastic bottle. Then, the dissolved oxygen was fixed by pouring it with 1 mL of MnSO4 creating orange-

brown particles within the water. The scientists considered this as the occurrence of precipitate where it is

believed that the more precipitate, the greater the dissolved concentration it has. Once the precipitate

particles settled, 1 mL of KI – NaN3 was then poured creating a clear colored orange-brown water sample,

repeating the mixing process.

As the scientist wait for the water sample to settle again, the titrant was prepared by adding 25 ml

of the standard Na2S2O3 solution into the buret. The titrant was slowly dispensed to the water sample until

the orange-brown color fades into a light yellow-brown color. A 1 mL of starch solution was then added

to the sample, transforming the color into a deep colored blue. Then, continue to slowly add drops of

Na2S2O3 solution until the deep-blue color fades away into a crystal clear water sample. The dissolved

oxygen concentration was then calculated expressing it in ppm O2.

Recommendation

The experimenters figured out that temperature affects the dissolved oxygen wherein as the

warmer the water, the less amount of oxygen absorbed. For the increase in temperature,

decreases the concentration dissolved oxygen. Looking into it in a larger set-up like in an aquatic

ecosystem, we can say that as the amount of sunlight decreases, the dissolved oxygen also

decreases. It is because sunlight is essential for the photosynthesis of a plant to produce oxygen.

And without sunlight, organisms will consume the oxygen in the to breathe and plant cannot

replenish the amount of oxygen due to lack of sunlight. Oxygen is an important component in

bodies of water for it is needed for metabolic processes of almost all living organisms within the
ecosystem. Overall, the experiment let the experimenters realized that oxygen and the processes

it undergoes is vital in for living organisms. The results accumulated in this paper can be

beneficial to businesses, water treatment companies, government agencies and communities by

demonstrating through titration, the amount of dissolved oxygen can be determined.

Conclusion

The oxygen content in the water is usually shown in milligrams per liter, Therefore the amount

of the dissolved oxygen in the given water sample is 8.16 mg/L. Since the concentration of

dissolved oxygen depends on the saltiness and the temperature of water, the dissolved oxygen

concentration in the water sample changes in an inverse relation when ambient temperature

change, and the concentration changes in a reverse manner when oxygen is dissolved with the

organic matter present in the water sample. To determine the concentration of dissolved oxygen

in the water sample, the Winkler test was utilized.