Analytical Chemistry Laboratory, (2019) 1-3

Analytical Chemistry Laboratory III

Iodometric testing of Methampiron (Antalgin) levels

Alfin Muhafidhah, Amalia Artamivea, Azzhar Azzahroh, Annis Istiqomah
Department of Pharmacy, Faculty of Health Science, University of Darussalam Gontor

A R T I C L E I N F O
A B S T R A C T
Article history:
Received ---
Accepted --- Iodimetric titration is a redox titration. Redox titrations involving iodine
Available online - can be done in two ways, namely direct titration (iodymetry) and indirect
titration (iodometry). Iodimetry is a redox titration that refers to a standard
iodine solution. In most direct titrations with iodine, an iodine solution is
used in the form of potassium iodide. This method produces efficient
Keywords:
Metampiron
products with relatively low cost and inexpensive equipment. In this study,
Iodimetric methampirone (antalgin) was used as the main sample to be tested. The
Kanji Indicator 5% working principle in this experiment is that several methamponone
Ttration (antalgin) sample solutions are reacted with the starch indicator (starch
solution) and titrated with an iodine solution to form a blue constant color.

1. Introduction community as an antiseptic drug. Iodine is also used as a

Volumetric analysis is a quantitative analysis
method that is very important to use in determining the
concentration of substances present in a solution. The
success of this volumetric analysis is largely determined
by the presence of the right indicators so that they can
show the exact endpoints of the titration. Titration can be
interpreted as a standard solution dropped from a burette
to a solution in an Erlenmeyer to be investigated in
volume. There are several types of titrations, one of
which is a redox titration, which is a titration based on
electron transfer between the titrant and the analyte.
Redox titrations involving iodine can be done in two
ways, namely direct titration (iodymetry) and indirect
titration (iodometry). Iodimetry titration is a direct
titration process using iodine as the standard solution.
The iodimetry method has advantages over the
spectrophotometric method. The iodimetry method can
produce efficient products with relatively low costs and
inexpensive equipment. While the spectrophotometric
method is less feasible because it is more expensive and
takes longer than iodimetry with a significant difference
inaccuracy.
Methampirone (antalgin) is a chemical in drugs that
can cause negative effects in the body of the wearer if
used in large quantities. Chemicals such as antalgin can
cause damage to the digestive organs, in the form of
thinning of the intestinal wall to cause bleeding.
Iodine solution can be used as a titrating agent
because iodine is a weak oxidizer, so it can titrate
substances that are strong reducing agents. Iodine also
has a very diverse function, among others, used by the
mixture in iodized salt to improve the quality of the salt which
will be consumed by humans. The addition of iodine to this salt
can prevent mumps, dwarf bodies, motor disturbances, mute,
deafness, and mental retardation. Iodine is also greatly needed
by the pharmaceutical industry as an iodine tincture ingredient.
The endpoint detection in this iodimetric titration is
carried out using a starch indicator that will give a blue color
when the endpoint is reached.
2. Tools and Materials
The tools used in testing the levels of methampirone
(Antalgin) iodimetrically are 25 ml burette, stative, clamps,
100 ml measuring cup, 100 ml beaker, 100 ml Erlenmeyer, 100
ml measuring flask, drop pipette, lumping and pestle, funnel
glass, spatula. The ingredients are distilled water, 0.1 N iodide
solution, 0.5% starch solution, antalgin 500 mg, Hcl solution.
3. Experiment Methods
This experiment was carried out using the Iodimetry
method which is also a direct redox titration, which is a
complete titration process using iodide solution as a standard.
The working principle of the methampirone sample reacted
with starch indicators and titrated with iodine to form a
constant blue color.
4. Observation Data
 Solution Titation volume (mL) If the change in color to a stable blue has occurred during
I 3,4 mL the titration then it indicates that the antalgin has finished
reacting and the endpoint of the titration has been reached.
II 2,1 mL The blue color is formed because in the starch solution there
are glucose units forming helical chains due to the presence
III 3,3 mL of configuration bonds in each glucose unit. This form can
cause the starch to form complexes with iodine molecules
Figure 1. Obserbvation data of titration results that can enter the spirals, causing a dark blue color.

5. Calculation
Determination of Metampiron Levels
1. Solution I ( 3,4 mL)
V iodida . N iodida . Be
= x 100%
gr Sampel

3,4 mL . 0,19 N . 16,67

= x 100% Figure 2. the results of the test of methamponite levels
0,4 gr
Furthermore, the levels obtained in each test are
= 2,692.205 % calculated. In the first experiment, the levels were
2. SolutionII ( 2,1 mL) 2,692,205%, in the second experiment 1,662.8325%, and in
V iodida . N iodida . Be the third experiment 2,613.0225%.
= x 100%
gr Sampel
7. Conclucion
2,1 mL . 0,19 N . 16,67 The secondary standard solution used in this practicum is
= x 100%
0,4 gr 0.1N iodine solution, an iodine solution is used because
iodine solution acts as a weak oxidizer and can be used to
= 1,662.8325 % titrate an antalgic solution which is a fairly strong reducing
3. Solution III ( 3,3 mL ) agent. The indicator used is a 0.5% starch solution because
V iodida . N iodida . Be the starch solution can cause a dark blue color that is
= x 100%
gr Sampel difficult to remove the color even though iodine
concentration is very small and the iodide molecule is
3,3 mL . 0,19 N . 16,67 strongly bound to the surface of beta-amylase. The results of
= x 100%
0,4 gr the levels obtained from the first experiment amounted to
2,692,205%, the second experiment amounted to 1
= 2,613.0225 % '662.8325%, and in the third experiment amounted to
2,613.0225%
6. Results and Discussion
Methampirone is a pyrazolone derivate that has a 8. References
strong analgesic-antipyretic effect. Works centrally on the Basset, J. dkk. 1994, Kimia Analisis Kuantitatif
brain to relieve pain, reduce fever, and cure rheumatic. Anorganik, Penerbit Buku Kedokteran EGC:
Quantitative analysis is the determination of levels of a Jakarta
chemical compound contained in a solution whose Gandjar, 1.G. 2007, kimia analisis farmasi, Pustaka
concentration is known. To determine these levels, the Pelajar: Yogyakarta.
titration method can be used. Hardjadi, w., 1993, Ilmu Kimia Analitik Dasar, PT.
In this experiment, the determination of the level of Gramedia: Jakarta
methampirone (antalgin) was done iodimetrically. Iodimetry Khopkar, S.M., 2002, Konsep Dasar Kimia Analitik,
is an oxidation-reduction titration that uses a standard UI Press: Jakarta
solution of iodine as a titrant in a neutral or slightly acidic
atmosphere. This titration is also called direct titration
because the reducing agent is oxidized directly with iodine
standard solution.
In the iodimetry practicum, the antalgin tablet samples were
determined by the direct titration method. To start this
experiment, first, the tools to be prepared are used, taken 3
antalgin tablets and crushed until smooth, then weighed and
taken as much as 400 mg, put into a measuring flask and
shaken then added 5 ml of concentrated HCl, adding this is
done to increase the acidity of the methampirone, because
the iodimetric titration is done in acidic conditions. Then
dissolve it with 50 ml of distilled water and shake until
homogeneous. Antalgin solution 50 ml pipette 10 ml then
put into a 250 ml Erlenmeyer, given a 0.5% starch indicator
solution dripped approximately 5 drops, used starch
indicator because it can form complex compounds with
iodide that is difficult to remove. Then it is titrated with 0.1
N iodide. Iodine solution is used in this titration because
iodine is a weak oxidizing agent which can titrate antalgin
which is a fairly strong reducing agent.