Lab Report on Determination of The Ka Value Of A Weak Acid 1

TABLE OF CONTENT

1.0 ABSTRACT ..................................................................................................................................... 3

2.0 INTRODUCTION........................................................................................................................... 4
3.0 OBJECTIVES ................................................................................................................................. 5
4.0 THEORY ......................................................................................................................................... 6
5.0 MATERIALS & APPARATUS ..................................................................................................... 9
6.0 METHODOLOGY ....................................................................................................................... 10
Titration Of Sodium Hydroxide, NaOH solution with Unknown Acid solution ....................... 10
7.0 RESULTS ...................................................................................................................................... 11
8.0 SAMPLE OF CALCULATIONS ................................................................................................ 13
9.0 DISCUSSION ................................................................................................................................ 15
10.0 CONCLUSION ........................................................................................................................... 17
11.0 RECOMMENDATIONS ............................................................................................................ 18
12.0 REFERENCES ............................................................................................................................ 19
13.0 APPENDIX .................................................................................................................................. 20

Lab Report on Determination of The Ka Value Of A Weak Acid 2

1.0 ABSTRACT

The strength of an acid is measured based on its ability to donate protons to base. The acid
ionization constant, Ka, is a quantitative measure of the strength of an acid. The Ka value is a
characteristic of an acid and can be used to identify an unknown acid. The main purpose of
the experiment is to find the Ka and identify the identity of the unknown acid solution. 15mL
of 0.1M unknown acid solution is diluted with 35mL distilled water producing 0.03M of
unknown acid solution. Standardized NaOH solution is added 1 mL by 1mL into the dilute
unknown acid solution. The acid-base titration curve is successfully plotted in a graph of pH
versus volume of sodium hydroxide, NaOH used in the experiment. The equivalence point of
the graph is 14.5mL with 8.2 corresponding pH. The half-equivalence point of the graph is
7.25mL with corresponding pH of 5.2. The experimental Ka value for Method 1 and Method
2 are 6.3096x10-6 and 1.7136x10-7 respectively. The identity of the unknown acid solution is
identified as pyridine with theoretical value Ka of 5.90x10-6. Method 1 with percentage error
of 6.9424% is more accurate compared to Method 2, 97.0956%. The objectives is
successfully obtained, therefore the experiment is successfully done.

Lab Report on Determination of The Ka Value Of A Weak Acid 3

2.0 INTRODUCTION

The relative acidity of a substance or a system is important in many situations, such as in the
quality of drinking water, food preservation, soil conditions for agriculture and physiological
functions. The strength of an acid is measured based on its ability to donate protons to base.
The purpose of titration is to determine the equivalence point of the reaction. The equivalence
point is reach when the added quantity of one reactant is the exact amount necessary for
stoichiometric reaction with another reactant. The half-equivalence point is determined for its
corresponding pH which also represent the pKa of the unknown acid solutions.

The acid ionization constant, Ka, is a quantitative measure of the strength of an acid.
The Ka value is a characteristic of an acid and can be used to identify an unknown acid. The
Ka value indicates the relative strength of an acid. The larger the Ka value, the stronger the
acid and vice versa. For the experiment, there are two method used to calculate experimental
Ka which are Acid-Base Titration and using the initial pH of the unknown acid solution.

Method 1 : Acid Base Titration (Half-Equivalence)

(Equation 4)

Method 2 : Initial pH of The Known Acid Solution

Ionic Equation for complete reaction with Base

HA + H2O → H3O+ + A-

The equation used to calculate Ka for Method 2,

[ ( )]
( )

Lab Report on Determination of The Ka Value Of A Weak Acid 4

3.0 OBJECTIVES

1) To determine the Ka value of an unknown acid solution.

2) To identify the actual identity of the unknown weak acid solutions.
3) To compare the calculated Ka value using Method 1 (Acid-Base Titration) and Method 2
(Measuring the pH of the weak acid).
4) To compare the experimental Ka values between two different method and theoretical Ka
value.
5) To plot the graph of pH versus volume of the sodium hydroxide, NaOH solution used for
the acid-base titration method (Method 1).

Lab Report on Determination of The Ka Value Of A Weak Acid 5

4.0 THEORY

There are two methods used to determine the Ka value for an acid which are
1) Acid-Base Titration,
2) Measuring the pH of the Acid Solution.

1) Acid-Base Titration
A titration curve is produced by plotting the graph of the pH of the solution versus the
volume of sodium hydroxide solution used. The equivalence point of the titration is reached
when all of the hydrogen (H+) ion in the acid [HA] completely reacted with the hydroxide
(OH-) ion in the base solution. On the titration curve, the equivalence point is read at the
center of the region where pH increases sharply. The half-equivalence point for the titration is
reached when exactly one half of the base required to completely neutralize the acid has been
added. At this point, the concentration of the acid [HA] in the solution is identical with the
concentration of its conjugate base [A].

[HA] = [A] (Equation 1)

Equation 1 can be simplified to yield equation 2,

Ka = [H3O+(aq)] (Equation 2)

Taking the negative logarithm of each side of Equation 2, Equation 3 can be derived

- log Ka = - log [H3O+(aq)] (Equation 3)

pKa = pH

Equation 3 indicates that the pKa for the acid is equal to the pH of the solution at the half
equivalence point. The Ka of the acid is determined from the pKa value as follows,

(Equation 4)

Lab Report on Determination of The Ka Value Of A Weak Acid 6

Sample Calculation Using Method 1
A weak acid is titrated with 0.1M sodium hydroxide, NaOH solution. The titration curve is
shown in Figure 1. Determine the Ka of the weak acid,

Half-equivalence
point, 5.81mL

Figure 1 - Titration Curve For Weak Acid Titrated With NaOH

The pH at half-equivalence point of the graph is the value for pKa. Half equivalence
point on the graph is at 5.81 mL with the corresponding pH of 4.2.

pKa = pH = 4.2 (at the half-equivalence point)

= 10-4.2 = 6.3096 x 10-5

2) Measuring the pH of the Acid Solution

From the pH of the acid solution (HA), we can determined the H+ and A- ion concentration,
which is related to the pH of solution by Equation 5,

[H3O+] = 10-pH (Equation 5)

By substituting [HA], [H3O+] and [A-] at equilibrium in to Equation 6 the value of Ka

can be calculated.

[ ( )]
( )

Lab Report on Determination of The Ka Value Of A Weak Acid 7

Sample Calculation Using Method 2
The pH of a 0.10M weak acid solution is 2.52 at 25⁰C. Calculate the Ka of the weak acid at
25⁰C. Initially, only the weak acid (HA) is present in the solution,. At equilibrium, a fraction
of weak acid (HA) molecules ionize, forming H3O+ and A-. The [HA] decreases while the
[H3O+] and [A-] increases until equilibrium is attained. The equilibrium [HA], [H3O+] and
[A-] are calculated as follows,

pH = 2.52
[H3O+] = 10-pH = 10-2.52 = 3.020 x 10-3 M

HA + H2O → H3O+ + A-
Initial 0.10M 0 0
Change -3.02 x 10-3 M -3.02 x 10-3 M -3.02 x 10-3 M
Equilibrium (0.10 – 3.02x10-3)M -3.02 x 10-3 M -3.02 x 10-3 M

[ ( )]

The calculated Ka value of the unknown acid is closest to that of benzoic acid, 6.28 x 10-5
(refer Appendix).

Lab Report on Determination of The Ka Value Of A Weak Acid 8

5.0 MATERIALS & APPARATUS

5.1) Material

1) 0.1 M Sodium hydroxide solution, NaOH

2) 0.1M Unknown acid solution
3) Distilled water, H2O

5.2) Apparatus

1) 50mL Burette
2) Retort Stand and clamp
3) 10mL Volumetric Pipette
4) 250 mL beaker
5) 100mL Measuring cylinder
6) pH meter
7) Glass Rod

Lab Report on Determination of The Ka Value Of A Weak Acid 9

6.0 METHODOLOGY

Titration Of Sodium Hydroxide, NaOH solution with Unknown Acid solution

1) 35.0mL of distilled water was transferred to the clean, dry 250 mL beaker. 15.0mL of
0.1M unknown acid solution is added to the beaker.
2) The new concentration of unknown acid solution is calculated and tabulated in result
table.
3) The dilute unknown acid solution was titrated with sodium hydroxide, NaOH solution
and the pH was recorded with 1 ml additions of NaOH solution into the unknown acid
solution.
4) Step 1 to 3 was repeated for the second trial to standardized the NaOH solution.
5) The graph of pH versus NaOH was plotted and the volume of the NaOH required to
neutralize the unknown acid solution was determined for each titration.
6) The equivalence point of the titration curve is determined.

Lab Report on Determination of The Ka Value Of A Weak Acid 10

7.0 RESULTS

Table 1 – Standardization of Sodium Hydroxide Solution Using Unknown Acid Solution

Titration 1 Titration 2
Concentration Of Unknown Acid (mol/L) 0.1 0.1
Volume Of Distilled Water (mL) 35 35
Volume Of Unknown Acid (mL) 15 15
Volume Of Unknown Acid + Distilled Water (mL) 50 50
New Concentration Of Unknown Acid (mol/L) 0.03 0.03
Volume Of NaOH to neutralize Unknown Acid 14.5 14.5

Table 2 – Titration Between Dilute Unknown Acid Solution and NaOH Solution
Volume of NaOH Titration 1 Titration 2
(mL) pH Temperature pH Temperature
(⁰C) (⁰C)
0 4.14 27.4 4.15 27.2
1 4.36 27.4 4.38 27.2
2 4.55 27.4 4.54 27.2
3 4.69 27.4 4.70 27.1
4 4.82 27.5 4.85 27.2
5 4.96 27.5 4.96 27.1
6 5.05 27.5 5.06 27.1
7 5.16 27.6 5.17 27.1
8 5.28 27.5 5.29 27.2
9 5.39 27.6 5.38 27.1
10 5.53 27.5 5.51 27.2
11 5.66 27.5 5.63 27.1
12 5.83 27.4 5.80 27.1
13 6.07 27.5 6.03 27.2
14 6.45 27.4 6.44 27.3
15 9.75 27.4 10.24 27.4
16 10.97 27.4 11.03 27.3
17 11.30 27.4 11.25 27.4

Lab Report on Determination of The Ka Value Of A Weak Acid 11

Table 3 – Percentage of Error For The Unknown Acid Solutions Predicted
Percentage Error
Predicted Unknown Acid Solution (%)
Method 1 Method 2
Pyridine (C5H5NH+) 6.9424 97.0956
Ethylenediamine (+H3NCH2CH2NH3+) 4343.3803 20.6761

Lab Report on Determination of The Ka Value Of A Weak Acid 12

8.0 SAMPLE OF CALCULATIONS

Sample Calculation For Dilute Concentration Of Unknown Acid Solution :-

( ) ( )
( )

Method 1 : Determine Ka Using Graph

Sample Calculation For Half-Equivalence Point:-

Locate the pH of the solution corresponding to the half-equivalence point at the Graph pH
versus NaOH.
pH(at half-equivalence point) = pKa = 5.2

Sample Calculation For Ka Using Equation 4 :-

Sample Calculation For Percentage Error :-

| |
| |
|( ) ( )|
|( )|

Lab Report on Determination of The Ka Value Of A Weak Acid 13

Method 2 : Determine Ka Using Initial pH of The Unknown Acid Solution

Sample Calculation For Average Initial pH of The Unknown Acid Solution

Sample Calculation For [H3O+] in the Unknown Acid Solution Using Equation 5 :-

HA + H2O → H3O+ + A-
Initial 0.03M 0 0
Change -7.1614x10-5 M -7.1614x10-5 M -7.1614x10-5 M
Equilibrium (0.03 - 7.1614x10-5) M -7.1614x10-5 M -7.1614x10-5 M

Sample Calculation For Ka of The Unkown Acid Solution Using Equation 6:-

[ ( )]

( )( )
( )

Sample Calculation For Percentage Error :-

| |
| |
|( ) ( )|
|( )|

Lab Report on Determination of The Ka Value Of A Weak Acid 14

9.0 DISCUSSION

The objective of the experiment is to determine the unknown acid provided by the lab
assistant by comparing the acid ionization constant, Ka obtained from the experiment and
theoretical value. The unknown acid solution with concentration of 0.1M is diluted with
water prior experiment. The concentration is reduced to 0.03M, however the amount of
acidic compound present remains the same. The reason for this dilution process is to slow
down the neutralization process and get a more exact data to graph the pH versus volume of
NaOH used to neutralize the acid. Water has the pH of 7 which will increase the pH of the
unknown acid solution and helps the acid to react with NaOH to neutralize their properties.

Unknown Acid Titrated With NaOH

12

10

8.28
Titration 1
pH

6
5.2 Titration 2
4 Linear (Titration 1)
Linear (Titration 2)
2

0
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
7.25
Volume Of NaOH (mL) 14.5

Figure 2 - Acid-Base Titration Curve

The graph shows that as the NaOH is added to the unknown acid solution which is
acidic, the pH of the solutions increases steadily until it achieves pH of about 6. After about
13.8mL of NaOH is added to the solution, the pH shoot up passing the neutral pH. After
about 16mL of NaOH solution is added to the solution, the graph shows a steady increase
towards the basic state of the solution. The equivalence point is determined at the graph at the
middle point of the line which increases rapidly at 13.8ml to 15mL. Thus, giving the value of
14.5mL as the equivalence point which the obtained pH of about 8.2. The half-equivalence
point is 7.25mL with corresponding pH of 5.2.

Lab Report on Determination of The Ka Value Of A Weak Acid 15

 The Ka obtained from the experiment are 6.3096x10-6 and 1.7136x10-7 for Method 1
and Method 2 respectively. By comparing the theoretical value of Ka from appendix, Method
1 yield towards pyridine with theoretical Ka value of 5.90x10-6 whereas Method 2 yield
towards ethylenediamine with theoretical Ka value of 1.42x10-7. Since the identity of the acid
is yet to be determined, the percentage error is calculated to observe the lowest error between
the experimental value and theoretical value.

Based on the percentage error calculated for both method, if the unknown acid is to be
concluded as pyridine the percentage error are 6.9424% and 97.0956% for Method 1 and
Method 2 respectively. If the unknown acid is to be concluded as ethylenediamine, the
percentage error are 4343.3803% and 20.6761% for Method 1 and Method 2 respectively.
Therefore, since by identifying the unknown acid solution as pyridine will yield the lowest
percentage of error, the unknown acid solution is concluded to be pyridine.

Method 1 shows a lower error with 6.9424% compared to Method 2 with 97.0956%.
Therefore, Method 1 is more accurate since only about 7% error which less 13 times
compared to Method 2. The inaccurateness of Method 2 might be due to the addition of water
to the 0.1M unknown acid solution. Distilled water has the pH of 7, the dilution of the
unknown acid making its pH increases. Since Method 2 depends on the pH value of the acid,
making the results varies depending on the amount of distilled water added. So, for the
unknown acid in the experiment, the students diluted it to 0.03M with initial pH of 4.14 and
4.15. This however resulting in big error as compared to the Ka theoretical value of pyridine.

As for Method 1, the Ka depends on the half-equivalence point on the graph which
obtained after the experiment is conducted. Since at the equivalence point, the acidic
compound of the unknown acid solution if fully neutralized by the sodium hydroxide, NaOH
solution used in the experiment making the amount of the acid in the solution is equal to its
conjugate base existing at the end point in Method 1 compared to assuming that the
concentration of the acid-base will follow the stoichiometric coefficient as in ionic equation
by using Method 2.

Lab Report on Determination of The Ka Value Of A Weak Acid 16

10.0 CONCLUSION

The acid-base titration curve is successfully plotted in a graph of pH versus volume of

sodium hydroxide, NaOH used in the experiment. The equivalence point of the graph is
14.5mL with 8.2 corresponding pH. The half-equivalence point of the graph is 7.25mL with
corresponding pH of 5.2. The experimental Ka value for Method 1 and Method 2 are
6.3096x10-6 and 1.7136x10-7 respectively. The identity of the unknown acid solution is
identified as pyridine with theoretical value Ka of 5.90x10-6. Method 1 with percentage error
of 6.9424% is more accurate compared to Method 2, 97.0956%. The objectives is
successfully obtained, therefore the experiment is successfully done.

Lab Report on Determination of The Ka Value Of A Weak Acid 17

11.0 RECOMMENDATIONS

Students should read and research more about the experiment before conducting the
experiment. This is to ensure the students able to conduct the experiment smoothly and
within the allocated time limit without facing confusion about the next step during the
experiment. Preparation for the data table should be done before the experiment to avoid
confusion and all the data for the experiment get to be recorded for the report.

The pH meter rod should be cleaned with distilled water before taking another reading
to ensure the accurateness of the pH of the solution though this might take a much longer
time for the experiment.

The eyes of the students who responsible in taking the reading of the burette should
be parallel to the meniscus of the sodium hydroxide, NaOH in order to avoid parallax error
from occurring. Since, this could lead to inaccurate data obtained due to the difference
volume of NaOH used for the experiment and tabulated in the data table.

The students should wear complete PPE such as gloves, goggles, face mask, covered
shoes and lab coat throughout the experiment to ensure the safety of the students. The
students should consistent in calculating the data. For example, the readings or the calculated
data should be in four decimal places.

Lab Report on Determination of The Ka Value Of A Weak Acid 18

12.0 REFERENCES

Caledon. (n.d.). Acid Base Neutralization Concentratioin Constant. Retrieved Nomber 1,

2017, from Enotes: http://www.enotes.com/homework-help/acid-base-neutralization-
concentration-constant-714646?en_action=hh-question_click&en_label=hh-
sidebar&en_category=internal_campaign

Determination of Ka Value of a weak acid. (n.d.). Retrieved November 2, 2017, from Scribd:
http://www.scribd.com/doc/287743209

Harvey, D. (2008). Analytical Chemistry 2.0. Retrieved November 4, 2017, from Analytical
Sciences Digital Library (ASDL):
http://www/asdlib.org/onlineArticles/ecourseware/Text_Files-files/Appendix.pdf

Harvey, D. (2008). Modern Analytical Chemistry. McGraw-Hill.

Keo, U. (2010, July 6). Determination of Ka of Weak Acids. Retrieved November 4, 2017,
from Scribd: http://www.scribd.com/doc/56128194

Martell, A. S. (1976). Critical Stability Constants. New York: Plenum Press.

Nave, R. (n.d.). Acid Base. Retrieved November 1, 2017, from Hyper Physics:
http://hyperphysics.phy-astr.gsu.edu/hbase/Chemical/acidbase.html

Schweitzer, N. (2012, March 5). Determination of Ka of Weak Acids Lab Report. Retrieved
November 4, 2017, from Scribd: http://www.scribd.com/doc/87709893

Lab Report on Determination of The Ka Value Of A Weak Acid 19

13.0 APPENDIX

Table 4 – Appendix For Ka Constant at 25⁰C and Zero Ionic Strength

Compound Conjugate Acid pKa Ka

Acetic Acid CH3COOH 4.757 1.75×10–5

Adipic Acid C4H8(COOH)2 4.42 3.8×10–5
5.42 3.8×10–6
Alanine +H3NCHCCH3OHO 2.348 (COOH) 4.49×10–3
9.867 (NH3) 1.36×10–10
Aminobenzene C6H5NH3+ 4.601 2.51×10–5
4-Aminobenzene Sulfonic C6H4NH3+O3S 3.232 5.86×10–4
Acid
2-Aminobenozic Acid C6H4COOHNH3+ 2.08 (COOH) 8.3×10–3
4.96 (NH3) 1.1×10–5
2-Aminophenol C6H4OHNH3+ 4.78 (NH3) 1.7×10–5
(T = 20⁰C) 9.97 (OH) 1.05×10–10
Ammonia NH4+ 9.244 5.70×10–10
Arginine +
H3NCHCCH2OHOCH2CH2N 1.823 (COOH) 1.50×10–2
HCNH3+NH2+ 8.991 (NH3) 1.02×10–9
[12.48] (NH2) [3.3×10–13]
Arsenic Acid H3AsO4 2.24 5.8×10–3
6.96 1.1×10–7
11.50 3.2×10–12
Asparagine (M = 0.1 M) +
H3NCHCCH2OHOCNH2O 2.14 (COOH) 7.2×10–3
8.72 (NH3) 1.9×10–9
Asparatic Acid +
H3NCHCCH2OHOCOHO 1.990 (a-COOH) 1.02×10–2
3.900 (b-COOH) 1.26×10–4
10.002 (NH3) 9.95×10–11
Benzoic Acid C6H5COOH 4.202 6.28×10–5
Benzylamine C6H5CH2NH3+ 9.35 4.5×10–10
Boric Acid H3BO3 9.236 5.81×10–10
(Pka2, Pka3:T = 20⁰C) [12.74] [1.82×10–13]
[13.80] [1.58×10–14]

Lab Report on Determination of The Ka Value Of A Weak Acid 20

 Compound Conjugate Acid pKa Ka

Carbonic Acid H2CO3 6.352 4.45×10–7

10.329 4.69×10–11
Catechol C6H5OHOH 9.40 4.0×10–10
12.8 1.6×10–13
Chloroacetic Acid ClCH2COOH 2.865 1.36×10–3
Chromic Acid H2CrO4 -0.2 1.6
(Pka1:T = 20⁰C) 6.51 3.1×10–7
Citric Acid C6H8O7 3.128 (COOH) 7.45×10–4
4.761 (COOH) 1.73×10–5
6.396 (COOH) 4.02×10–7
Cupferrron C6H5NNOOH 4.16 6.9×10–5
(M = 0.1 M)
Cysteine +
H3NCHCCH2OHOSH [1.71] (COOH) [1.9×10–2]
8.36 (SH) 4.4×10–9
10.77 (NH3) 1.7×10–11
Dichloracetic Acid Cl2CHCOOH 1.30 5.0×10–2
Diethylamine (CH3CH2)2NH2+ 10.933 1.17×10–11
Dimethylamine (CH3)2NH2+ 10.774 1.68×10–11
Dimethylglyoxime C2H2NOHHON 10.66 2.2×10–11
12.0 1.×10–12
Ethylamine CH3CH2NH3+ 10.636 2.31×10–11
Ethylenediamine +
H3NCH2CH2NH3+ 6.848 1.42×10–7
9.928 1.18×10–10
Ethylenediaminetetraacetic NH++HNCOOHCOOHHOOC 0.0 (COOH) 1.0
Acid HOOC 1.5 (COOH) 3.2×10–2
(Edta) (M = 0.1 M) 2.0 (COOH) 1.0×10–2
2.66 (COOH) 2.2×10–3
6.16 (NH) 6.9×10–7
10.24 (NH) 5.8×10–11
Formic Acid HCOOH 3.745 1.80×10–4

Lab Report on Determination of The Ka Value Of A Weak Acid 21

 Compound Conjugate Acid pKa Ka

Fumaric Acid COOHC2H2HOOC 3.053 8.85×10–4

4.494 3.21×10–5
Glutamic Acid +
H3NCHCCH2OHOCH2COH 2.33 (a-COOH) 5.9×10–3
O 4.42 (l-COOH) 3.8×10–5
9.95 (NH3) 1.12×10–10
Glutamine +
H3NCHCCH2OHOCH2CNH2 2.17 (COOH) 6.8×10–3
(M = 0.1 M) O 9.01 (NH3) 9.8×10–10

Glycine +H3NCH2COOH +
H3NCHCHOHO 2.350 (COOH) 4.47×10–3
9.778 (NH3) 1.67×10–10
Glycolic Acid HOOCH2COOH 3.831 (COOH) 1.48×10–4
Histidine +
H3NC3H2CHCCH2OHO+HN 1.7 (COOH) 2.×10–2
(M = 0.1 M) NH 6.02 (NH) 9.5×10–7
9.08 (NH3) 8.3×10–10
Hydrogen Cyanide HCN 9.21 6.2×10–10
Hydrogen Fluoride HF 3.17 6.8×10–4
Hydrogen Peroxide H2O2 11.65 2.2×10–12
Hydrogen Sulfide H2S 7.02 9.5×10–8
13.9 1.3×10–14
Hydrogen Thiocyanate HSCN 0.9 1.3×10–1
8-Hydroxyquinoline C9H6NH+OH 4.91 (NH) 1.2×10–5
9.81 (OH) 1.6×10–10
Hydroxylamine HONH3+ 5.96 1.1×10–6
Hypobromous Acid HOBr 8.63 2.3×10–9
Hypochlorous Acid HOCl 7.53 3.0×10–8
Hypoiodous Acid HOI 10.64 2.3×10–11
Iodic Acid HIO3 0.77 1.7×10–1
Isoleucine +
H3NCHCCHOHOCH3CH2CH 2.319 (COOH) 4.80×10–3
3 9.754 (NH3) 1.76×10–10
Leucine +
H3NCHCCH2OHOCHCH3CH 2.329 (COOH) 4.69×10–3
3 9.747 (NH3) 1.79×10–10

Lab Report on Determination of The Ka Value Of A Weak Acid 22

 Compound Conjugate Acid pKa Ka

Lysine (M = 0.1 M) +
H3NCHCCH2OHOCH2CH2C 2.04 (COOH) 9.1×10–3
H2NH3+ 9.08 (a-NH3) 8.3×10–10
10.69 (e-NH3) 2.0×10–11
Maleic Acid C2H2(COOH)2 1.910 9.1×10–3
6.332 9.1×10–3
Malic Acid C2H3OH(COOH)2 3.459 (COOH) 9.1×10–3
5.097 (COOH) 9.1×10–3
Malonic Acid CH2(COOH)2 2.847 9.1×10–3
5.696 9.1×10–3
Methionine (M = 0.1 M) +
H3NCHCCH2OHOCH2SCH3 2.20 (COOH) 9.1×10–3
9.05 (NH3) 9.1×10–3

Methylamine CH3NH3+ 10.64 9.1×10–3

2-Methylanaline C6H4CH3NH3+ 4.447 9.1×10–3
4-Methylanaline C6H4CH3NH3+ 5.084 9.1×10–3
2-Methylphenol C6H4CH3OH 10.28 9.1×10–3
4-Methylphenol C6H4CH3OH 10.26 9.1×10–3
Nitrilotriacetic Acid NH+C3H3(COOH)3 1.1 (COOH) 9.1×10–3
(T = 20 Oc) 1.650 (COOH) 9.1×10–3
(Pka1: M = 0.1 M) 2.940 (COOH) 9.1×10–3
10.334 (NH3) 9.1×10–3
2-Nitrobenzoic Acid C6H4COOHNO2 2.179 9.1×10–3

3-Nitrobenzoic Acid C6H4COOHNO2 3.449 9.1×10–3

4-Nitrobenzoic Acid C6H4COOHO2N 3.442 3.61×10–4

2-Nitrophenol C6H4OHNO2 7.21 6.2×10–8

3-Nitrophenol C6H4OHNO2 8.39 4.1×10–9

4-Nitrophenol C6H4OHO2N 7.15 7.1×10–8

Nitrous Acid HNO2 3.15 7.1×10–4
Oxalic Acid H2C2O4 1.252 5.60×10–2
4.266 5.42×10–5

Lab Report on Determination of The Ka Value Of A Weak Acid 23

 Compound Conjugate Acid pKa Ka

1,10-Phenanthroline C12H8NH+N 4.86 1.38×10–5

Phenol C6H5OH 9.98 1.05×10–10

Phenylalanine +H3NCHCCH2OHOC6H5 2.20 (COOH) 6.3×10–3
9.31 (NH3) 4.9×10–10

Phosphoric Acid H3PO4 2.148 7.11×10–3

7.199 6.32×10–8
12.35 4.5×10–13
Phthalic Acid C6H4(COOH)2 2.950 1.12×10–3
5.408 3.91×10–6
Piperdine C5H10NH2+ 11.123 7.53×10–12
Proline NH2+C4H7COOH 1.952 (COOH) 1.12×10–2
10.640 (NH) 2.29×10–11
Propanoic Acid CH3CH2COOH 4.874 1.34×10–5
Propylamine CH3CH2CH2NH3+ 10.566 2.72×10–11
Pryidine C5H5NH+ 5.229 5.90×10–6
Resorcinol C6H4OHOH 9.30 5.0×10–10
11.06 8.7×10–12
Salicylic Acid C6H4COOHOH 2.97 (COOH) 1.1×10–3
13.74 (OH) 1.8×10–14
Serine +
H3NCHCCH2OHOOH 2.187 (COOH) 6.50×10–3
9.209 (NH3) 6.18×10–10
Succinic Acid HOOCC2H4COOH 4.207 6.21×10–5
5.636 2.31×10–6
Sulfuric Acid H2SO4 strong —
1.99 1.0×10–2
Sulfurous Acid H2SO3 1.91 1.2×10–2
7.18 6.6×10–8
D-Tartaric Acid HOOCC2H2COOHOHOH 3.036 (COOH) 9.20×10–4
4.366 (COOH) 4.31×10–5
Threonine +
H3NCHCCHOHOOHCH3 2.088 (COOH) 8.17×10–3
9.100 (NH3) 7.94×10–10

Lab Report on Determination of The Ka Value Of A Weak Acid 24

 Compound Conjugate Acid pKa Ka

Thiosulfuric Acid H2S2O3 0.6 3.×10–1

1.6 3.×10–2
Trichloroacetic Acid Cl3CCOOH 0.66 2.2×10–1
(M = 0.1 M)
Triethanolamine (HOCH2CH2)3NH+ 7.762 1.73×10–8
Triethylamine (CH3CH2)3NH+ 10.715 1.93×10–11
Trimethylamine (CH3)3NH+ 9.800 1.58×10–10
Tris (Hydroxymethyl) (HOCH2)3CNH3+ 8.075 8.41×10–9
Amino Methane
(TRIS Or THAM)
Tryptophan +
H3NCHCC7H5CH2OHOHN 2.35 (COOH) 4.5×10–3
(M = 0.1 M) 9.33 (NH3) 4.7×10–10

Tryosine +
H3NCHCC6H4CH2OHOOH 2.17 (COOH) 6.8×10–3
(Pka1: M = 0.1 M) 9.19 (NH3) 6.5×10–10
10.47 (OH) 3.4×10–11
Valine +
H3NCHCCHOHOCH3CH3 2.286 (COOH) 5.18×10–3
9.718 (NH3) 1.91×10–10
All values are from Martell, A. E.; Smith, R. M. Critical Stability Constants, Vols. 1–4.
Plenum Press: New York, 1976

Lab Report on Determination of The Ka Value Of A Weak Acid 25