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EUROIMMUN Labordiagnostika
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Strategy for Determination of Autoantibodies against Cell Nuclei (ANA) and Cytoplasm Components
Screening test (indirect immunofluorescence) Human epithelial cells (HEp-2) and primate liver
Cell nuclei, homogen. Cell nuclei, peripheral Nucleoplasm, dotted Nucleoplasm, granular
Interphase: homogeneous Nuclear membrane HEp-2: 46 / 92 dots HEp-2: 6 - 20 dots HEp-2: 2 - 6 dots Granular with HEp-2: fine granular, nucleoli pos. HEp-2: granular HEp-2: coarse granular Different degrees Different degrees
granular chromo- of reactivity, of reactivity.
Chromosome bands: positive positive Liver: weak reaction Liver: several dots Liver: positive or negative some bands or neg. liver: weak fluorescence Liver: clumpy, granular Liver: strong fluorescence mitotic cells neg. mitotic cells pos.
AC-1 AC-11/AC-12 AC-3 AC-6 AC-7 AC-2 AC-4 AC-4 AC-5 AC-13 AC-14
IFT: Crithidia luciliae (Anti-dsDNA) ELISA: Anti- ELISA: ANA Screen, Anti-ENA ELISA: ANA Screen, Anti-ENA ELISA: ANA
ELISA: ANA Screen, Anti-ENA EUROLINE: Profiles ANA, DFS70 Profiles, Anti-SS-A / B Profiles, Anti-nRNP / Sm Screen Profiles
ELISA: Anti-dsDNA, -histones, EUROLINE: Profiles ANA, liver ProfilePlus 2, Anti-centromeres EUROLINE: Profiles ANA, EUROLINE:
liver diseases EUROLINE:
-nucleosomes diseases (Anti-gp210) EUROLINE: Profiles ANA, myositis, systemic sclerosis EUROLINE: Profiles ANA, EUROLINE:
EUROLINE: Profiles (Anti-CENP B) (Anti-Sp100, -PML) ANA Profiles,
RIA: Anti-dsDNA Anti-DFS70 Anti-ENA, liver diseases Anti-ENA ANA Profiles
Nucleoli positive Cytoplasm filamentous Cytoplasm homogeneous Systemic lupus erythematosus Progressive systemic sclerosis (diff. form)
Autoantibodies against Prevalence Autoantibodies against Prevalence
Nucleoli stressed, Nucleoli Nucleoli Nucleoli Nucleoli granular, HEp-2: Needle-shaped fibre HEp-2 and liver: fine fibres. HEp-2: Fibre slings. Double-stranded DNA(dsDNA) 60 – 90 % Fibrillarin (U3-nRNP) 5 – 10 %
nucleoplasm mitotic cells: Homogeneous Homogeneous Single-stranded DNA (ssDNA) 70 – 95 % Scl-1 (75-kDa- / 13 %
homogeneous homogeneous fine granular coarse granular single dots bundles. Liver: Bile canaliculi Droplets in the mitotic cells Liver: Fibres in the parenchyma
100-kDa-main antigen) (10 % / 7 %)
Nucleosomes 50 – 70 %
Scl-70 25 – 75 %
RNA 50 %
RNA-polymerase I 4%
RNA-helicase A 6%
Ku, incl. overlap syndrome
Histones 50 – 80 % 25 – 50 %
with PM / DM
U1-nRNP 15 – 40 % NOR (NOR-90) rare
Sm 5 – 40 % 7 - 2-RNP (ThTo) rare
SS-A (Ro) 20 – 60 % Ro-52 28 %
SS-B (La) 10 – 20 %
Rheumatoid arthritis
AC-8 AC-9 AC-10 AC-10 AC-15 AC-16 AC-17 AC-19 AC-19 PCNA-like 3%
Autoantibodies against Prevalence
Ku 10 %
Anti-ribosomal Histones 15 – 50 %
Anti-Scl-70 Anti-PM-Scl Anti-fibrillarin Anti-RNA poly- Anti-NOR Anti-actin Anti-vimentin Anti-tropomyosin Anti-PL-7 / PL-12 Ribosomal P proteins 10 %
merase I P-proteins Single-stranded DNA 8%
Ro-52 38 %
PSS, diff. PM, DM, PSS, diff. PSS, diff. PSS, diff. PSS, diff. AIH Infections and inflammations Myastenia gravis, CU, MC SLE PM, DM (Cardiolipin 40 – 60 %)
U1-nRNP 3%
Ro-52 5%
Drug-induced lupus
ELISA: Profiles, ELISA: ANA Screen Profiles, EUROLINE: EUROLINE: ELISA: Profiles, Inclusion body myositis
EUROLINE: Autoantibodies against Prevalence
Anti-Scl-70 Anti-PM-Scl Profiles ANA, Profile Anti-rib. EUROLINE: Autoantibodies against Prevalence
Profile systemic P-proteins Histones 95 %
EUROLINE: EUROLINE: Profiles ANA, systemic sclerosis systemic Myositis Profiles cN-1A 39 %
sclerosis EUROLINE:
Profiles myositis, systemic sclerosis (Anti-RP11 / RP155) sclerosis Profiles Neonatal lupus erythematosus
Polymyositis and dermatomyositis
Autoantibodies against Prevalence
SS-A 100 % Autoantibodies against Prevalence
PM-Scl (PM-1), incl. overlap
HEp-2: mitotic cells positive Cytoplasm granular Primary Sjögren’s syndrome syndrome with PSS
24 – 55 %
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HA_1500_I_UK_B09, 07 / 2017