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Unit-I Introduction
1. Define microbiology?

2. Listout the contribution of Robert Koch in microbiology?

3. Contribution of Alexander flaming in microbiology?

4. Contrbutionof Antony van Leeuwenhoek in microbiology?

5. Listout the important contribution of Louis Pasteur in microbiology?

6. Mention the important contribution Karl Landsteiner in microbiology?

7. Describe the Koch ‘s postulates?

8. Enlist the contribution of Joseph Lister?

9. Write importance of Paul Ehrich?

10. Enlist the importance of microbiology for nurses?

11. describe the concept of microbiology?

12. write the principles of microbiology?

13. Scope of medical microbiology?

14. listout the important discoveries in the period of modern microbiology?

15. Define microbes?

16. Mycoplasms?

17. define virus

18. define bacteria?

19. Define bacteriology?

20. Enlist classification of bacterial taxonomy?

21. What is fermentation process?

22. Write any four mile stones in microbiology development?

23. Write about usefulness of knowledge of microbiology in nursing.

24. Define immunogy

25. Define virology

26. Define mycology?

27. Define parasitology?

28. What is medical asepsis?

29. What is surgical asepsis?

30. What is Anaerobic

31. What is aerobic

32. Define tyndallisation?

33. Define fermentation?

34. What is Pasteurization?

35. List out any four nobel prize winners for microbiology?

Unit-II Micro-Organisms

1. define bacteria?

2. morphology of bacteria with diagram?

3. Classify the bacteria on basis of their shape?

4. Classify the bacteria on characteristics arrangement of their cell?

5. Enlist the classification of bacteria?

6. Write different types of microorganisms.

7. vibrios
8. cytoplasm

9. ribosomes

10. meososomes

11. flagella.

12. Write about microscopy with diagram?

13. Write the structure of bacteria?

14. Enlist the shape of bacteria?

15. Write the size and form of bacteria?

16. List out the pathogenic and non-pathogenic organisms

17. Define spore

18. Define cytoplasm

19. Define ribosomes

20. Define fimbriae

21. Koch postulates?

22. Define bacterial flagella

23. List out the anaerobic bacteria classification?

24. write down the mechanism of action of antimicrobial agents?

25. write about antimicrobial resistance?

26. Prokaryotypes?
27. Eukaryotypes?

Unit III infection and its transmission

1. Define the infection with examples?

2. write down the classification of infections?

3. List out the sources of infections?

4. enlist the sources of infection in humans?

5. define primary infection?

6. define secondary infection?

7. What do you understand about Hospital acquired infection?

8. define focal infection?

9. define latent infection?

10. define iatrogenic infection?

11. List out the route of infection?

12. Differentiate between direct and indirect transmission?

13. Listout the common nosocomial infections and causative agents?

14. write down the domestic animals and rodents of infectious diseases?

15. write down the insects of infectious diseases?

16. what type of infection transmitted by ingestion?

17. Define zoonoses with examples of zoonotic diseases?

18. List out the types of infectious diseases?

19. define the antibiotic susceptibility test?

20. Enlist the methods of transmission of infection?

21. What is growth curve?

22. Enlist the phases of growth curve?

23. Write the growth and multiplication of bacteria?

24. write about bacterial nutrition?

25. What are the laboratory methods for identification of microorganisms?

26. identification of bacteria?

27. What is bacterial growth curve?

28. Define lag phase?

29. Define log phase?

30. Define stationary phase?

31. Define decline phase?

32. listout the principles of specimen collection and disposable?

33. enlist the types of specimens?

34. Catalase test

35. Urease test

36. Citrate utilization test

37. Oxidase test

38. Indole test

39. Sugar fermentation test

40. Triple sugar iron test

41. coagulase test

42. Methyl red test?

43. voges-proskauer test?

44. urease test?

45. methyl blue reduction test?

46. define antibiotic sensivity test?

47. discuss about Kirby-baer method of antibiotic sensitivity test?

48. Listout the types of antibiotic sensivity test?

49. listout the antibiotic sensivity test?

50. agar dilution test?

51. broth dilution test?

52. write about the Collection and transport of clinical samples to laboratory?

53. Write about Hospital Infection control procedure

54. What is the role of nurses in Hospital Infection control .

Unit-IV Immunity
1. Define the antigen and antibody?

2. What is immunity?

3. Listout the types of antigen?

4. Cold chain?

5. What is autoimmunity?

6. Define precipitation reaction with examples?

7. What is vaccine?

8. How the vaccines

9. Write about immunoglobulin A(IgA)?

10. Write about immunoglobulin MIgM)??

11. Write about immunoglobulin DIgD)??

12. Write about immunoglobulin EIgE)??

13. define agglutination reaction?

14. Immunofluorescence test

15. Define hypersensitivity ?

16. Define & classify hypersensitivity ?

17. Write down the COOMBS AND GEL classification of hypersensitivity ?

18. Innate immunity

19. Active immunity

20. Passive immunity

21. Differentiate between active and passive immunity?

22. Define immunoprophylaxisis?

23. National immunization schedule?

24. Write different types of vaccines?

25. Write down the adverse reactions for each vaccine?

26. How to transport the vaccines?

27. Write down the general practices for vaccine storage and handling?

28. Listout the general features of antigen –antibody reactions?

29. differentiate the stages of antigens and antibody reactions?

30. What is tube agglutination test?

31. Latex agglutination test?

32. Humoralimmunity?

33. Cell mediated immunity?

34. Write about natural active immunity?

35. Write about artificial active immunity?

36. Listout the killed vaccines?

37. Listout the live vaccines?

38. Listout the bacterial vaccines?

39. Listout the viral vaccines?

40. Define the type I hypersensitivity reactions with examples?

41. Define the type II hypersensitivity reactions with examples?

42. Define the type III hypersensitivity reactions with examples?

43. Define the type IV hypersensitivity reactions with examples?

44. Define atopy?

45. Anaphylaxis?

46. Immediate and delayed hypersensivity?

47. What is the functions of immunoglobulins?

Unit –V The control and destruction of micro-organisms

1. Write the chain of infection

2. Define cross infection ?
3. Hospital acquired infection control team?
4. Prevention of Hospital acquired infection ?
5. Treatment of Hospital acquired infection ?
6. Write down the prevention and control of infection?
7. Bio-safety and waste management.
8. Define Sterilization
9. Differentiate between sterilization and disinfection?
10. Define Disinfection
11. Define Asepsis
12. Define Antiseptics
13. Write physical methods of sterilization?
14. Write dry heat sterilization?
15. DefineBatericidal agents
16. Define Bacteriostatic agents
17. Define Decontamination?
18. Define Cleaning?
19. Enlist the physical agents?
20. Listout the chemical agents?
21. Listout the chemical disinfectants?
22. Classification of biomedical waste management?
23. Enlist the categories of biomedical waste management?
24. Write down the health hazards of biomedical waste management?
25. Listout the sources of biomedical waste management?
26. Writ down the Colour coding and type of container used for disposal of biomedical waste
27. Write down the segregation of waste in color coded bags?
28. Boiling?
29. Autoclave
30. Filtration?
31. Aldehydes
32. Phenol
33. Write down Standard biosafety measures ?
34. List out the principles and methods of microbial control
35. Sterilization by dry heat
36. Sterilization by moist heat
37. chemicals and radiation.
38. Sterilization by Disinfection
39. Sterilization by natural gases
40. What type of chemicals used and preparation of lotions
41. What is Chemotherapy
42. what is antibiotic?
43. What is the uses of antibiotics with examples?
44. Differentiate between Medical and surgical asepsis
45. How to Control the spread of infection
46. What is Pasteurization

Unit-VI Introduction to Laboratory techniques

1. What is Microscope

2. List out the various types Microscope

3. Draw the microscope showing parts

4. List out the various Parts Microscope

5. Write a note on care of microscope?

6. Write the morphology of bacterial colony?

7. List out the Uses of Microscope

8. How to handling and care of microscope

9. Write about light or optical microscope and its types?

10. Write about electron microscope and its types?

11. Write about light or optical microscope and its types?

12. Write about Bright field or compound microscope

13. Write about phase contrast microscope

14. Write about dark field microscope

15. Write about fluorescent microscope

16. Hanging drop preparation procedure

17. What is Simple stain

18. Write staining and examination of slides

19. Simple staining procedure
20. Gram staining procedure

21. Listout the reagents of Gramstaining

22. Write down the composition of Gram stains

23. What is acid fast stain

24. Ziehl-Neelsenstaining/ Acid fast staining procedure

25. List the techniques of Acid fast staining

26. Listout the reagents of Ziehl-Neelsenstaining

27. Albert stain

28. Composition of Albert stain

29. define culture?

30. Define culture media:?
31. Listout the types of culture media?
32. Write the preparation and examination of smears.
33. listout the culture characteristics?
34. Listout the types of culture media ?

35. Write Inoculation of culture media

36. Write down the difference between enriched and enrichment media?
37. Write down the difference between aerobic and anaerobic culture methods?

38. What is complex media?

39. What is simple media?

40. What is selective media?

41. What is differential media?

42. What is indicator media?

43. What is transport media?

44. Write the composition of liquid media?

45. Write the composition of solid media?

46. What is streak culture?

47. What is lawn culture media?

48. Methyl red test?

49. voges-proskauer test?

50. urease test?

51. methyl blue reduction test?

52. define antibiotic sensivity test?

53. discuss about Kirby-baer method of antibiotic sensitivity test?

54. Listout the types of antibiotic sensivity test?

55. listout the antibiotic sensivity test?

56. write down the mechanism of action of antimicrobial agents?

57. write about antimicrobial resistance?

58. agar dilution test?

59. broth dilution test?

60. write about the Collection and transport of clinical samples to laboratory?

61. numerate the Bacterial colonisation on various parts of the body?

62. listout the principles of specimen collection and disposable?

63. enlist the types of specimens?

64. identification of bacteria?

65. Catalase test

66. Urease test

67. Citrate utilization test

68. Oxidase test

69. Indole test

70. Sugar fermentation test

71. Triple sugar iron test

72. coagulase test

73. Methyl red test?

74. voges-proskauer test?

75. urease test?

76. methyl blue reduction test?

77. define antibiotic sensivity test?

78. discuss about Kirby-baer method of antibiotic sensitivity test?

79. Listout the types of antibiotic sensivity test?

80. listout the antibiotic sensivity test?

81. agar dilution test?

82. broth dilution test?

83. define the antibiotic susceptibility test?

84. list out principles of gram stain

85. Enlist the staining techniques?

86. alberts stain

87. fluorescent microscope

88. Write about nutrient agar?

89. Write about Blood agar?
90. Write aboutMac Conckey agar?
91. What is hanging drop preparation?