Operating Manual Photoflex WTW

Operating manual
pHotoFlex /
pHotoFlex Turb
Handheld photometer
ba75491d04 07/2006
Accuracy when The use of advanced technology and the high quality standard of our
going to press instruments are the result of continuous development. This may result
in differences between this operating manual and your instrument. Al-
so, we cannot guarantee that there are absolutely no errors in this man-
ual. Therefore, we are sure you will understand that we cannot accept
any legal claims resulting from the data, figures or descriptions.
Firmware Part of the process of consequently improving our products is the

Method data continuous further development of the range of photometric tests
Analysis specifications supplied and the meter firmware. All current data for the pHotoFlex /
Operating manual pHotoFlex Turb can be found on the Internet under http://
www.WTW.com.
You can easily transfer new firmware to your instrument with the aid of
the AK 540/B cable and a PC. More detailed information can be found
in the appendix of the detailed operating manual on the CD-ROM
provided.
Copyright © 2006, WTW GmbH

Reprinting - even as excerpts - is only allowed with the explicit written
authorization of WTW GmbH, Weilheim.
Printed in Germany.
pHotoFlex / pHotoFlex Turb Contents
1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.1 General features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2 Keypad . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
1.3 Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
1.4 Socket field . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
1.5 LabStation (optional) . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.1 Authorized use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
2.2 General safety instructions . . . . . . . . . . . . . . . . . . . . . . . 14
3 Commissioning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
3.1 Scope of delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
3.2 Power supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
3.3 Connecting the LabStation . . . . . . . . . . . . . . . . . . . . . . . 19
3.4 Initial commissioning . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
4 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
4.1 Switching on the meter . . . . . . . . . . . . . . . . . . . . . . . . . . 23
4.2 Inserting a cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
4.3 General operating principles . . . . . . . . . . . . . . . . . . . . . 27
4.3.1 Operating modes . . . . . . . . . . . . . . . . . . . . . . . . 27
4.3.2 Navigation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
4.3.3 Navigation example 1: Setting the language . . . 30
4.3.4 Navigation example 2: Setting the date and time 31
4.3.5 Menu overview . . . . . . . . . . . . . . . . . . . . . . . . . 33
4.4 System settings (System menu) . . . . . . . . . . . . . . . . . . 35
4.4.1 Measured value memory . . . . . . . . . . . . . . . . . . 36
4.4.2 Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
4.4.3 Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
4.4.4 Date/time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
4.5 Photometry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
4.5.1 General information . . . . . . . . . . . . . . . . . . . . . . 40
4.5.2 Settings for photometric measurements . . . . . . 41
4.5.3 Measuring the concentration . . . . . . . . . . . . . . . 42
4.5.4 Blank value (reagent blank value) . . . . . . . . . . . 43
4.5.5 Measuring the absorbance/transmission . . . . . 45
4.5.6 Zero adjustment . . . . . . . . . . . . . . . . . . . . . . . . 46
4.5.7 Programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
4.5.8 Analysis timer . . . . . . . . . . . . . . . . . . . . . . . . . . 49
4.5.9 Timer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
4.5.10 Measuring diluted samples . . . . . . . . . . . . . . . . 50
4.6 pH value / ORP voltage . . . . . . . . . . . . . . . . . . . . . . . . . 51
4.6.2 Measuring the pH value . . . . . . . . . . . . . . . . . . 52
4.6.3 Measuring the ORP voltage . . . . . . . . . . . . . . . 53
4.6.4 Settings for pH and ORP measurements . . . . . 53
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Contents pHotoFlex / pHotoFlex Turb
4.6.5 Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
4.6.6 Carrying out the TEC and NIST/DIN calibration
procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
4.7 Turbidity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
4.7.2 Aligning and marking a cell . . . . . . . . . . . . . . . . 62
4.7.3 Measuring turbidity . . . . . . . . . . . . . . . . . . . . . . 63
4.7.4 Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
4.8 Storing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
4.8.1 Storing measurement datsets . . . . . . . . . . . . . . 69
4.8.2 Filtering measurement datsets . . . . . . . . . . . . . 70
4.8.3 Displaying measurement datsets . . . . . . . . . . . . 71
4.8.4 Downloading the measurement datsets to the
RS232
interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
4.8.5 Erasing stored measurement datasets . . . . . . . 72
4.9 Transmitting data (RS 232 interface) . . . . . . . . . . . . . . . 72
4.9.1 Connecting a PC/external printer . . . . . . . . . . . . 72
4.9.2 Configuring the RS232 interface . . . . . . . . . . . . 73
4.9.3 Selecting the output format of datasets . . . . . . . 73
4.9.4 Transmitting data . . . . . . . . . . . . . . . . . . . . . . . . 75
4.10 Reset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
4.10.1 Resetting the system settings . . . . . . . . . . . . . . 76
4.10.2 Resetting the photometer settings . . . . . . . . . . . 77
4.10.3 Resetting the pH settings . . . . . . . . . . . . . . . . . . 77
4.11 Meter information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
4.12 Software update . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
4.13 Administrating user-defined methods . . . . . . . . . . . . . . . 79
4.13.1 Administrating user-defined programs with a
terminal program . . . . . . . . . . . . . . . . . . . . . . . . 80
5 Maintenance, cleaning, disposal . . . . . . . . . . . . . . . . . 83

5.1 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
5.1.1 Inserting/exchanging the batteries . . . . . . . . . . . 83
5.1.2 Retrofitting the accumulator pack . . . . . . . . . . . 84
5.2 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
5.2.1 Cleaning the cell shaft . . . . . . . . . . . . . . . . . . . . 85
5.2.2 Cleaning the cells . . . . . . . . . . . . . . . . . . . . . . . 85
5.3 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
6 What to do if... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
6.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
6.2 Photometry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
6.4 Turbidity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
7 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
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pHotoFlex / pHotoFlex Turb Contents
7.1 General data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91

7.1.1 pHotoFlex (Turb) . . . . . . . . . . . . . . . . . . . . . . . . 91
7.1.2 LabStation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
7.2 Photometry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
7.4 Turbidity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94
8 Accessories, options . . . . . . . . . . . . . . . . . . . . . . . . . . . 95
8.1 WTW accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95
8.1.1 Connection cable: . . . . . . . . . . . . . . . . . . . . . . . 95
8.2 Optional extensions of the pHotoFlex (Turb) . . . . . . . . . 97
9 Lists . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99
10 Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
Appendix: Firmware update . . . . . . . . . . . . . . . . . . . . . . . 107
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Contents pHotoFlex / pHotoFlex Turb
6 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Overview
1 Overview
1.1 General features
The compact pHotoFlex (Turb) handheld precision photometer en-
ables you to carry out the following measurements quickly and reliably:
! Photometric measurements
– Concentration measurements
– Absorbance measurements
– Transmission measurements
! pH measurements
! Turbidity measurements (pHotoFlex Turb).
The pHotoFlex (Turb) handheld meter provides the maximum degree

of operating comfort, reliability and measuring certainty for all applica-
tions.
The proven MultiCal ® calibration procedure supports you when cali-

brating for pH measurements and the AutoRead function enables pre-
cise pH measurements.
1 Keypad
2 Display
3 Cell shaft
(folded out for a 16 mm cell to be inserted)
4 Socket field
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Overview pHotoFlex / pHotoFlex Turb
Note
If you need further information or application notes, you can obtain the
following material from WTW:
! Application reports
! Primers
! Safety datasheets.
You will find information on available literature in the WTW catalog or

via the Internet.
1.2 Keypad
Key functions Select the measuring mode

<M> (long keystroke):
– Photometry
– Turbidity
– pH & ORP
Select the measured parameter within a measur-
ing mode
<M> (short keystroke):
– pH & ORP: pH, ORP
– Photometry:
Concentration, Absorbance, % Transmission
– Turbidity: no measured parameters selectable
Start calibration (measuring modes, pH & ORP,
Turbidity)
Start zero adjustment or blank value measure-
ment using the Photometry \ Adjustment menu
(measuring mode, Photometry)
<CAL/ZERO>
8 ba75491d04 07/2006
In the Photometry measuring mode: Select a pro-

gram for concentration measurement
<PROG>
Open menus / confirm entries /
. start measurement
<START/ENTER>
Call up the Configuration menu
(all settings are made here)
<MENU>
In the Photometry measuring mode, measured
parameter, Concentration:
switch over between available citation forms
<FORM>
In the Photometry measuring mode, measured
parameter, Concentration:
Switch over between available units <UNIT>
Switch the measuring instrument on/off
<ON/OFF>
Output display contents to RS232 interface (e.g.

PRT
print)
<PRT>
Open the Store menu <STO>,
Quick storing <STO> <STO>
Highlight menu items or selection

Set values
<▲>, <▼>
Switch to the next higher menu level /
cancel input
<ESC>
Note
Keys with an additional number printed on are assigned doubly.
This enables to directly enter numbers in special menus. Thus, you
can, for example, conveniently enter the date and time via the number
keys.
ba75491d04 07/2006 9
1.3 Display
The graphic display shows all information of the current measurement
in the measured value display. The illumination enables to read the dis-
play even in the darkness.
Example Photometry \ Concentration Measuring mode \ measured

parameter
1.29 mg/l Measured value (with unit)
2: A5/25 MC NH4-N
Program and citation form
16 mm 0.20 - 8.00 mg/l Diameter of the cell and
01.02.05 15:12 measuring range
Status line with date and time
1.4 Socket field
1 2 3 4 5
Identifying the 1 pH electrode

connectors
2 pH temperature sensor
3 Power pack
4 Contacts for operation on the LabStation
5 RS232 serial interface
10 ba75491d04 07/2006
1.5 LabStation (optional)

With the LabStation, which is available as an accessory, you can con-
veniently use the pHotoFlex (Turb) in the laboratory.
Laboratory operation with the LabStation enables the following addi-
tional functions:
! With photometric measurements, the zero measurement is retained
even after switching the pHotoFlex (Turb) off and on again
! You can connect a barcode reader for the simplified calling up of
programs
! The LSdata software serves to easily enter user-defined programs
! Line power operation is possible to save the batteries or accumula-
tor pack
! The accumulator pack in the pHotoFlex (Turb) is automatically
charged as soon as the meter is placed in the LabStation.
LabStation
Fig. 1-1 LabStation
ba75491d04 07/2006 11
12 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Safety
2 Safety
This operating manual contains basic instructions that you must follow
during the commissioning, operation and maintenance of the meter.
Consequently, all responsible personnel must read this operating man-
ual carefully before working with the meter. The operating manual must
always be available within the vicinity of the meter.
Target group The meter was developed for work in the field and in the laboratory.
We assume that, as a result of their professional training and experi-
ence, the operators will know the necessary safety precautions to take
when handling the chemicals of photometric test sets.
The personnel responsible for the commissioning, operation and main-

tenance must have the necessary qualifications for this work. If the per-
sonnel do not have the required skills they have to be instructed.
Furthermore, it must be ensured that the personnel read and complete-
ly understand the present operating manual.
Safety instructions The individual chapters of this operating manual use the following safe-
ty instructions to indicate various types of danger:
Caution
indicates instructions that must be followed precisely in order to
avoid the possibility of slight injuries or damage to the instrument
or the environment.
Further notes
Note
indicates notes that draw your attention to special features.
Note
indicates cross-references to other documents, e.g. operating manu-
als.
ba75491d04 07/2006 13
Safety pHotoFlex / pHotoFlex Turb
2.1 Authorized use

This meter is authorized exclusively for carrying out the following mea-
surements in the field and laboratory:
! Analysis of substances in water and aqueous solutions
using round cells
! Concentration measurement
! Absorbance and transmission measurement
! Measurement of pH value and ORP
! Turbidity measurement (pHotoFlex Turb only).
The technical specifications as given in chapter 7 TECHNICAL DATA must

be observed. Only the operation and running of the meter according to
the instructions given in this operating manual is authorized. Any other
use is considered to be unauthorized.
2.2 General safety instructions

This instrument is built and inspected according to the relevant guide-
lines and norms for electronic measuring instruments (see chapter
7 TECHNICAL DATA).
It left the factory in a safe and secure technical condition.
Opening the photometer or adjustment, maintenance and repair work

must only be performed by specialist personnel authorized by the man-
ufacturer.
The only exceptions to this are the activities described in chapter

5 MAINTENANCE, CLEANING, DISPOSAL. Non-compliance results in the
loss of warranty claims.
Follow the points listed below when operating the photometer:

! Follow the local safety and accident prevention regulations
! Observe the enclosed instructions of reagents and accessories
! Observe the regulations when dealing with dangerous substances
! Follow the operating instructions at the workplace
! Use only original spare parts.
Function and operating The smooth functioning and operational safety of the meter can only be
safety guaranteed if the generally applicable safety measures and the specific
safety instructions in this operating manual are followed during opera-
tion.
14 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Safety
The smooth functioning and operational safety of the meter can only be
guaranteed under the environmental conditions that are specified in
chapter 7 TECHNICAL DATA.
If the instrument was transported from a cold environment to a warm

environment, the formation of condensate can lead to the faulty func-
tioning of the instrument. In this event, wait until the temperature of the
instrument reaches room temperature before putting the instrument
back into operation.
Safe operation It is the responsibility of the operator to continuously observe the over-
all technical condition (externally recognizable deficits and damage as
well as alterations to the operational behavior) of the meter.
If safe operation is no longer possible, the instrument must be taken out

of service and secured against inadvertent operation!
Safe operation is no longer possible if the meter:
! has been damaged in transport
! has been stored under adverse conditions for a lengthy period of
time
! is visibly damaged
! no longer operates as described in this manual.
If you are in any doubt, please contact the supplier of the instrument.
Caution
Danger of eye damage by visible and invisible LED radiation. In
the cell shaft there are light emitting diodes (LED) of the 1M class.
Do not look at the radiation using optical instruments.
With normal, authorized use there is no hazard.
Obligations of the The purchaser of this meter must ensure that the following laws and
operator guidelines are observed when using dangerous substances:
! EEC directives for protective labor legislation
! National protective labor legislation
! Safety regulations
! Safety datasheets of the chemical manufacturers.
ba75491d04 07/2006 15
Safety pHotoFlex / pHotoFlex Turb
16 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Commissioning
3 Commissioning
3.1 Scope of delivery
! Handheld photometer, pHotoFlex Turb or pHotoFlex
! 4 batteries, 1.5 V type AA (in the battery compartment)
! Optional: Accumulator pack and power pack with Euro plug
and exchange plugs for USA, UK, and Australia
! Optional: LabStation
! 1 empty cell 16 mm
! 2 empty cells 28 mm
with label to mark the cell for turbidity measurements
! AMCO®-Clear turbidity standard (pHotoFlex Turb only)
! Microfiber cloth to clean the meter
! Compact operating manual and short operating manual
! CD-ROM with
– detailed operating manual
– photometry analysis manual with analysis specifications
– software to program user-defined methods
Note
The optional parts of the scope of delivery are available as accessories
(see section 8.1).
3.2 Power supply

You can operate the meter either with batteries, accumulator pack or a
power pack. The power pack supplies the meter with low voltage
(9 V DC). At the same time, the accumulator pack is charged. The ac-
cumulator pack is charged even while the meter is switched off.
The LoBat display indicator appears when the batteries or accumulator

pack is nearly discharged.
Charging time of the approx. 36 hours.

accumulator pack
Caution
The line voltage at the operating site must lie within the input volt-
age range of the original power pack (see chapter 7 TECHNICAL DA-
TA).
Caution
ba75491d04 07/2006 17
Commissioning pHotoFlex / pHotoFlex Turb
Use original power packs only (see chapter 7 TECHNICAL DATA).
Note
The accumulator pack should not be completely discharged. If you do
not operate the instrument for a longer period of time you should charge
the accumulator pack every six months.
Automatic switchoff The meter has an automatic switch-off function in order to save the bat-
teries or accumulator pack (see section 4.4).
Display illumination During operation with batteries or accumulator pack the meter automat-
ically switches off the display illumination if no key is pressed for
30 seconds. The illumination is switched on with the next keystroke
again. The display illumination can also be switched off completely (see
section 4.4.2).
Note
Power pack and accumulator pack are available as an accessory (see
section 8.1).
Connecting the power 4

pack (optional)
2 3
1 If necessary, replace the Euro plug (1) on the power pack (2)
by the country-specific plug suitable for your country.
2 Connect the plug (3) to the socket (4) of the photometer.
3 Connect the power pack to an easily accessible power socket.
18 ba75491d04 07/2006
3.3 Connecting the LabStation

Note
The LabStation is available as an accessory (see section 8.1).
In order to use the functions of the LabStation for operation in the lab-
oratory, connect the LabStation and place the pHotoFlex (Turb) in the
LabStation.
Connecting the LabSta-

tion (optional)
5
4
2 3
1 If necessary, replace the Euro plug (1) on the power pack (2)
by the country-specific plug suitable for your country.
2 Connect the plug (3) to the socket (4) of the LabStation.
3 Connect a PC, printer or barcode reader to the socket (5) of the
LabStation as necessary.
Note
When the pHotoFlex (Turb) is operated in the LabStation, a barcode
reader can be connected. A barcode reader enables to select program
numbers in a simplified manner.
Recommended barcode readers can be found in section 8.2.
For the interface of your meter, set the same baud rate that is set for
the barcode reader (see section 4.4.3).
The baud rate of the barcode reader is given in the operating manual
of your barcode reader.
ba75491d04 07/2006 19
4 Connect the power pack to an easily accessible power socket.

5 Place the pHotoFlex (Turb) in the LabStation.
LabStation
6 Connect a pH electrode to the socket on the pHotoFlex (Turb)

through the cut-out (6) as necessary.
20 ba75491d04 07/2006
3.4 Initial commissioning

Perform the following activities:
! For
– accumulator operation: insert the accumulator pack (see
section 5.1.2)
– line power operation and charging the accumulator pack: connect
the power pack (see section 3.2)
– operation with LabStation: connect the LabStation and place the
pHotoFlex (Turb) in the LabStation (see section 3.3)
! Switch on the meter (see section 4.1)
! Set the language as necessary (see section 4.3.3)
! Set the date and time as necessary (see section 4.3.4)
Note
When you set the language, date and time according to the mentioned
sections of this operating manual you will quickly become familiar with
the simple operation of the pHotoFlex (Turb).
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22 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Operation
4 Operation
4.1 Switching on the meter
Switching on Press the <ON/OFF> key.
For 30 seconds, Start menu appears with a selection of the measuring
modes. The measuring mode last selected is highlighted.
The status line indicates the meter designation and the version number
of the software.
Start
Photometry
Turbidity
pH & ORP
i pHotoFlex V 0.24
After a few seconds, the meter automatically switches to the measuring

mode and measured parameter used last.
The measured value display appears (here, e.g. measuring mode Pho-
tometry).
Photometry \ Concentration
i Select program
with <PROG>
01.02.05 15:12
With <M> (long pressure) change the measuring mode.

With <M> (short pressure) toggle between the different measured pa-
rameters in the selected measuring mode.
Switching off Press the <ON/OFF> key.
Automatic switchoff The meter has an automatic switchoff function in order to save the bat-
teries or accumulator pack (see section 4.4). The automatic switchoff
switches the meter off if no key is pressed for an adjustable period.
The automatic switchoff is not active
! if the power is supplied by the power pack (optional),

! if the power is supplied by the LabStation (optional),
! if the Timer or Analysis timer function is on.
ba75491d04 07/2006 23
Operation pHotoFlex / pHotoFlex Turb
Display illumination During operation with batteries or accumulator pack the meter automat-
with battery and accu- ically switches off the display illumination if no key is pressed for
mulator pack operation 30 seconds. The illumination is switched on again with the next key-
stroke.
4.2 Inserting a cell

To be able to insert cells in the pHotoFlex (Turb), the cell shaft has to
be prepared to take in a cell.
1 Push the dust cover (1) upward.

The cell shaft for 28 mm cells is open.
! Insert a 28 mm cell (see below)
! Insert a 16 mm cell (see page 25)
Inserting a 28 mm cell
2 Insert the cell so that it is positioned on the bottom of the cell
shaft.
The cell is ready to be measured.
3 For turbidity measurement:

Align the cell (see section 4.7.2).
24 ba75491d04 07/2006
Inserting a 16 mm cell
1 Put the fold-out cell shaft (2) in an upright position until it locks
into place.
2 Pull up the height adapter (3).

The cell shaft is extended.
3 Open the external light cover (4) of the cell shaft.
ba75491d04 07/2006 25
4 Insert the 16 mm cell (marking points forward) so that it is po-

sitioned on the bottom of the cell shaft.
5 Close the external light cover (4).
The cell is ready to be measured.
Note
For optimum measurement results, the cell must always be covered by
the external light cover. Otherwise, external light can falsify the mea-
surement result.
26 ba75491d04 07/2006
4.3 General operating principles

This section contains basic information on the operation of the
pHotoFlex (Turb).
Operating elements, An overview of the operating elements and the display is given in
display section 1.2 and section 1.3.
Operating modes, An overview of the operating modes of the pHotoFlex (Turb) and the
navigation navigation through menus and functions can be found in section 4.3.1
and section 4.3.2.
4.3.1 Operating modes
The instrument has the following operating modes:

! Measurement
The display indicates measurement data in the measured value dis-
play
! Calibration
The display indicates a calibration process with calibration informa-
tion,
or a process to carry out a zero adjustment
! Data transmission
The meter transmits measuring datasets or calibration records to the
serial interface
! Configuration
The display indicates a menu with further menus, settings and func-
tions
4.3.2 Navigation
Measured value display In the measured value display, you can

! select a measuring mode with <M> (long pressure)
! select a measured parameter in the active measuring mode (e. g.
pH <−> mV) with <M> (short pressure)
! open the menu with <MENU>
! switch to the superordinate Start menu with <ESC>.
ba75491d04 07/2006 27
Menus and dialogs The menus for settings and dialogs in courses contain further sub-
menus. The selection is made with the <▲> <▼> keys.
The current selection is displayed in reverse video.
! Menus
The name of the menu is displayed at the upper edge of the frame.
Menus are opened by confirming with <START/ENTER>. Example:
Configuration
Photometry
Turbidity
pH & ORP
System
Info
! Settings
Settings are indicated by a colon. The current setting is displayed on
the right-hand side. With <START/ENTER>, the selection of the
possible settings is opened. Subsequently, the setting can be
changed with <▲> <▼> and <START/ENTER>.
Example:
System
Language: Deutsch
Beep: Off
Illumination: On
Contrast: 48 %
Temperature unit: °C
Switchoff time: 30 min
! Functions
Functions are designated by the name of the function. They are im-
mediately carried out by confirming with <START/ENTER>.
Example: display the Calibration record function
(in the pH & ORP / Calibration menu).
pH & ORP
Calibration record
Cal. type: AutoCal
TEC
Calibration interval: 007 d
Unit for slope: mV/pH
:i 2.00 4.01 7.00 10.01
! Messages
Information or instructions are marked by the i symbol. They can-
not be selected.
28 ba75491d04 07/2006
Example:
pH & ORP
i Buffer recognition TEC The i symbol indicates
i Immerse sensor in buffer 1 info texts, e.g.
messages, notes or instruc-
tions
Set temperature: 25 °C
Continue
Note
The principles of navigation are explained in the two following sections
by reference of examples:
! Setting the language (section 4.3.3)
! Setting the date and time (section 4.3.4).
ba75491d04 07/2006 29
4.3.3 Navigation example 1: Setting the language
Note
The following example describes in the language of the country how to
set the language. On delivery, English is set as the language in the
pHotoFlex (Turb). During initial commissioning, the language is set in
the menu, Configuration / System / Language.
1 In the measured value display:

Open the Configuration menu with <MENU>.
The instrument is in the configuration mode.
2 Select the System menu with <▲> <▼>.
3 Open the System menu with <START/ENTER>.
4 Select the Language menu with <▲> <▼>.
System
Language: Deutsch
Store
Display
Reset
Interface
Continue ...
5 Open the setting of the Language with <START/ENTER>.
System
Language: Deutsch
Store
Display
Reset
Interface
Continue ...
6 Select the required language with <▲> <▼>.

7 Confirm the setting with <START/ENTER>.
The setting is active. The menu is displayed in the selected lan-
guage.
30 ba75491d04 07/2006
8 To make further settings, switch to the next higher menu level

with <ESC>.
or
Switch to the measured value display with <M> (short pres-
sure).
The instrument is in the measurement mode.
4.3.4 Navigation example 2: Setting the date and time
The meter has a clock with a date function. The date and time are indi-
cated in the status line of the measured value display. When storing
measured values and calibrating, the current date and time are auto-
matically stored as well.
Numerals are generally entered via the number keys.
The correct setting of the date and time and date format is important for
the following functions and displays:
! Current date and time
! Calibration date
! Identification of stored measured values.
Therefore, check the time at regular intervals.
Note
After a fall of the supply voltage (empty batteries or accumulator pack),
the date and time are reset to 01.01.2003, 00:00 hours.
Setting the date, time The data format can be switched from the display of day, month, year
and date format (dd.mm.yy) to the display of month, day, year (mm/dd/yy or mm.dd.yy).
1 In the measured value display:

Open the Configuration menu with <MENU>.
The instrument is in the configuration mode.
2 Select and confirm the System / Continue ... / Date/time menu
with <▲> <▼> and <START/ENTER>.
Date/time
Time: 14:53:40
Date: 30.10.03
Date format: dd.mm.yy
ba75491d04 07/2006 31
3 Select and confirm the Time menu with <▲> <▼> and
<START/ENTER>.
A display for the entry of numerals with the number keys opens
up.
Time
14:53:40
4 Enter the time using the number keys.

The digit to be changed is displayed underlined.
Note
In the case of wrong entries, you can cancel the procedure with <ESC>.
After canceling with <ESC>, it is possible to enter all digits once again.
The new digits are only taken over by confirming with <START/EN-
TER>.
5 Confirm the setting with <START/ENTER>.

The time is set.
6 Set the current Date as necessary. The setting is made similar-
ly to that of the time.
7 Change the date format as necessary.
8 To make further settings, switch to the next higher menu level
with <ESC>.
or
Switch to the measured value display with <M> (short pres-
sure).
The instrument is in the measurement mode.
32 ba75491d04 07/2006
4.3.5 Menu overview
Photometry Measured parameter Concentration

% Transmission
Absorbance
Programs
Dilution
Analysis timer On
Off
Reset
Turbidity i No settings required.
pH & ORP Measured parameter pH
ORP
Calibration Calibration
record
Cal. type TEC
NIST/DIN
Calibration inter- 1 ... 999 d
val
Unit for slope mV/pH
%
Man. temperature -20 ... +130 °C
Temperature unit °C, °F
Reset
Timer
ba75491d04 07/2006 33
System Language Deutsch

English
Français
Español
Measured value Display
memory
RS232 download
Data filter Filter
ID
PROG
Date
Delete
i 4 of 1000 occupied
i Filter: No filter
Display Illumination Auto off
On
Off
Contrast 0 ... 100 %
Brightness 0 ... 100 %
Reset
Interface Baud rate 1200, 2400, 4800,
9600, 19200
Output format ASCII
CSV
Continue ... / Time hh:mm:ss
Date/time
Date
Date format dd.mm.yy
mm.dd.yy
mm/dd/yy
Continue ... / 10, 20, 30, 40, 50 min,
Switchoff time 1, 2, 3, 4, 5, 10, 15, 20, 24 h
Continue ... / On
Beep Off
Info
34 ba75491d04 07/2006
4.4 System settings (System menu)

The following instrument features and general functions can be found
in the Configuration / System menu:
! Language selection (Language)
! Memory and database functions (Store)
! Display settings (Display)
! Restore basic settings (Reset)
! Configuration of the interface for PC/printer (Interface)
! Setting the date/time (Date/time)
! Setting the switch-off time (Switchoff time)
! Setting the keyboard sound (Beep)
Settings/functions The settings can be found in the Configuration / System menu.

To switch to the Configuration menu, press the <MENU> key.
Menu item Setting Description

Language Deutsch Select the language (see
English section 4.3.3)
Français
Español
Store Display Memory and database
RS232 down- functions
load (see section 4.8.2)
Data filter
Delete
Display Illumination Switch on/off the display
Contrast illumination (see
section 4.4.2)
Brightness
Reset - Resets all system settings
to default
(see section 4.10.1)
Interface Baud rate Baud rate of the data in-
Output format terface
(see section 4.4.3)
Continue ... / Time Settings of time and date
Date/time Date (see section 4.3.4)
Date format
ba75491d04 07/2006 35
Menu item Setting Description

Continue ... / 10, 20, 30, 40, The automatic switchoff
Switchoff time 50 min, switches the meter off if
1, 2, 3, 4, 5, 10, no entry is made for a
15, 20, 24 h specified period of time
(Switchoff time). This
saves the batteries or ac-
cumulator pack.
Continue ... / On Switch on/off the beep on
Beep Off keystroke
4.4.1 Measured value memory
In the Measured value memory menu, you find functions to display and
edit the stored measurement datasets:
! Display the measurement datsets on the screen (Display)
! Download the measurement datsets to the RS232 interface (RS232
download)
! Set up filter rules for the stored measurement datsets (Data filter)
! Erase all stored measurement datsets (Delete)
! Information on the number of occupied memory locations
The settings can be found in the Configuration / System / Measured val-

ue memory menu.
Settings/functions Menu item Setting/func- Description

tion
Display - Displays in pages all mea-
surement datasets that cor-
respond to the filter settings.
Further options:
! Scroll through the
datasets with <▲> <▼>.
! Output the displayed
dataset to the interface
with <PRT>.
! Quit the display with
<ESC>.
36 ba75491d04 07/2006
Menu item Setting/func- Description

tion
RS232 download - Downloads to the interface
all measurement datasets
that correspond to the filter
settings. The download is
ordered according to the
date and time.
The process can take sever-
al minutes. To terminate the
process prematurely, press
<ESC>.
Data filter see Allows to set filter criteria in
section 4.8.2 order to display and down-
load datasets to the inter-
face.
Delete - Erases the entire contents
of the measuring data mem-
ory, independent of the filter
settings.
Note:
All calibration data remains
stored when performing this
action.
All details on the subjects of memory and stored data is found in

section 4.8.2.
ba75491d04 07/2006 37
4.4.2 Display
In the Configuration / System / Display menu, you set the display fea-
tures:
! Switching on/off the display illumination (Illumination)
! Display contrast (Contrast)
The settings can be found in the Configuration / System / Display menu.

Settings Menu item Setting Description

Illumination Auto off The display illumination is
automatically switched off if
no key has been pressed
for 30 seconds.
On Switches the display illumi-
Off nation on or off permanent-
ly (see section 4.5.8)
Contrast 0 ... 100 % Changes the display con-
trast
Brightness 0 ... 100 % Changes the display bright-
ness
4.4.3 Interface
In the Interface menu, you set the features of the interface:

! Transmission speed (Baud rate)
! Output format (Output format)
The settings can be found in the Configuration / System / Interface

menu.

Baud rate 1200, 2400, Baud rate of the data
4800, 9600, interface
19200
Output format ASCII Output format for data
CSV transmission
For details, see section 4.9
38 ba75491d04 07/2006
4.4.4 Date/time
In the Configuration / System / Continue ... / Date/time menu, you set

the system clock:
! Current time (Time)

! Current date (Date)
! Format of the date display (Date format)
The settings can be found in the Configuration / System / Continue ...

Date/time menu.

Time hh:mm:ss Enter the time with the
number keys
Date Enter the date with the
number keys
Date format dd.mm.yy Settings of time and date.
mm.dd.yy
mm/dd/yy
ba75491d04 07/2006 39
4.5 Photometry
4.5.1 General information
Photometric measurements serve to determine chemical substances in

liquid samples. For this determination, the substance to be determined
has to be present in a form that is suitable for photometric measure-
ment. At the same time, possible disturbing factors have to be exclud-
ed.
Before measurement, the sample has to be pretreated in order to bring

the substance to be determined into the form that is suitable for mea-
surement and at the same time exclude disturbing factors.
Pretreatment of the sample is described in the analysis specification.
In a simple case, pretreatment can be to dissolve a solid substance in

water; it can, however, also include chemical conversions, e. g. a di-
gestion.
The chemicals required in the analysis specification are available as

test sets.
Note
Suitable analysis specifications for test sets can be found in the pho-
tometry analysis manual (on CD-ROM).
There you will also find further instructions on handling chemicals and
on how to proceed when applying the analysis specifications.
Methods and the corresponding method data for many test sets are
stored as programs in the pHotoFlex (Turb). A program number is as-
signed to each program.
By entering the program number or by using a barcode reader the

stored method data is loaded.
You can look up an overview of the available methods in the photome-

try analysis manual and display it on the screen of the pHotoFlex (Turb)
(see section 4.5.7).
You can measure the following parameters with the pHotoFlex (Turb):
! Concentration [mg/l]
! % Transmission []
! Absorbance []
40 ba75491d04 07/2006
Preparatory activities Perform the following preparatory activities when you want to measure:
1 Clean the cells before filling them with sample and also before
measuring as necessary (see section 5.2.2).
The cells must be absolutely clean and free of scratches.
2 For measurement, place the pHotoFlex (Turb) on a horizontal
surface.
4.5.2 Settings for photometric measurements
For photometric measurements, the following settings are available in

the Configuration / Photometry menu:
! Setting the measured parameter
! Displaying a list of all programs
! Setting the dilution factor
! Switching on or off the analysis timer
! Resetting the settings for photometric measurements
The settings can be found in the Configuration / Photometry menu.


Measured parame- Concentration Measured parameters in
ter % Transmission the Photometry measuring
mode
Absorbance
Programs Display all programs with
the corresponding pro-
gram data (see
section 4.5.7).
Dilution Set the dilution factor (see
section 4.5.10)
Analysis timer On Switch on/off the analysis
Off timer (see section 4.5.8)
Reset Reset all settings for the

Photometry measuring
mode (see section 4.10.3)
ba75491d04 07/2006 41
4.5.3 Measuring the concentration
1 Press the <M> key (long pressure) repeatedly until the Pho-
tometry measuring mode is selected.
2 Press the <M> key (short pressure) repeatedly until the mea-
sured variable, Konzentration is selected.
First concentration measure- Second and all further

ment with the pHotoFlex (Turb) concentration measurements
Photometry \ Concentration Photometry \ Concentration
i Select program i Select program

with <PROG> with <PROG>
or with ▲ ▼
1: A5/25 MC NH4-N
16 mm 0.20 - 6.51 mg/l
01.02.05 15:12 01.02.05 15:12
Note
From the second concentration measurement, the data of the program
last used is automatically displayed here.
With <▲> <▼> you can quickly switch between the ten programs last
used.
To select a program, you can also read in the program number of an

analysis specification with a barcode reader (see section 8.2). The fol-
lowing step 3 is then skipped. You can directly start measurement.
The program number of the test is given in the analysis specification,
on the list of available programs and on the packing of some tests (un-
der the barcode).
3 Open the Program number display with <PROG>,

enter the required program number with the number keys and
confirm with <START/ENTER>.
or (from the second concentration measurement):
Select a program out of the last ten programs with <▲> <▼>.
The program data is displayed.
Note
If a program number is selected that requires a measured blank value,
the menu automatically guides to the blank value measurement.
42 ba75491d04 07/2006
i Insert sample
i Start measurement
with <START>
1: A5/25 MC NH4-N
16 mm 0.20 - 6.51 mg/l
01.02.05 15:12
4 Insert the cell (see section 4.2).

5 Start the measurement with <START/ENTER>.
Measurement is started. The result is displayed.
[BV] A blank value measured by
the user is used
0.74 mg/l
1: A5/25 MC NH4-N
16 mm 0.20 - 6.51 mg/l
01.02.05 15:12
4.5.4 Blank value (reagent blank value)
A blank value is required for every concentration measurement.

For some programs (methods) for concentration measurement, the
blank values are already stored in the photometer. They are used au-
tomatically. For all other programs, the blank value has to be deter-
mined separately before the first measurement.
Each stored reagent blank value can be replaced by a blank value de-
termined by the user.
Note
You will find more information on blank values in the photometry anal-
ysis manual.
A blank value is always stored for the program that has just been called
up. It remains stored until it is erased (menu item, Delete blank value)
or overwritten.
The Reset function erases all blank values measured by the user and
restores the blank values stored in the factory.
If a blank value measured by the user is stored for a program, this blank
value is used for measurement. The usage of the blank value mea-
sured by the user is documented and also indicated in the measured
value display.
ba75491d04 07/2006 43
Measuring the blank 1 Press the <M> key (long pressure) repeatedly until the Pho-
value tometry measuring mode is selected.
sured variable, Concentration is selected.
3 Select a program with <PROG> as necessary.
Note
The following measurement of the blank value applies only to the se-
lected program. If no program is selected, the message i No program
selected. appears on the display.
4 Open the adjustment menu with <CAL/ZERO>.
Photometry \ Adjustment
Zero adjust.
Measure blank value
Delete blank value
5 Using <▲> <▼> and <START/ENTER>, select and start the

Measure blank value function.
The menu-guided blank value measurement starts.
Follow the instructions on the display.
Measure blank value

i 2: A5/25 MC
i Cell = 16 mm
i Insert blank sample
Start measurement
6 Insert a cell with blank sample (see section 4.2).

7 Start the measurement of the blank value with <START/EN-
TER>.
After measuring, the result of the blank value measurement is
displayed and stored.
The result is displayed as i Blank value measurement suc-
cessful! or i Blank value measurement erroneous!
44 ba75491d04 07/2006
Measure blank value

i 2: A5/25 MC
i Cell = 16 mm
i Absorbance = 0.301
i Blank value mea-
surement
successful!
8 Confirm the result with <START/ENTER>.

The blank value measurement is completed.
The meter is ready to measure.
or:
Discard the result with <ESC>.
Subsequently, carry out a new blank value measurement.
4.5.5 Measuring the absorbance/transmission
Note
The transmission measurement is not described separately in the fol-
lowing example as it operates in exactly the same way as the absor-
bance measurement. The result of the measurement is displayed in %
Transmission.
sured variable, Absorbance or % Transmission is selected.
Photometry \ Absorbance
i Select cell
with ▲ ▼
16 mm 610 nm
01.02.05 15:12
3 Select the cell diameter with <▲> <▼> and confirm with
<START/ENTER>.
4 Select the wavelength with <▲> <▼> and confirm with
<START/ENTER>.
ba75491d04 07/2006 45
i Insert sample
i Start measurement
with <START>
16 mm 610 nm
01.02.05 15:12
5 Clean the cell (see section 5.2.2).

7 Start the measurement with <START/ENTER>.
The measurement result is displayed when the measurement
is finished.
0.532
16 mm 610 nm
01.02.05 15:12
4.5.6 Zero adjustment
The zero adjustment, i. e. measuring and storing the absorbance of a

cell filled with water, is necessary after the meter is switched on.
Additionally, we recommend to carry out a zero adjustment if the ambi-
ent temperature has changed.
Only perform the zero adjustment against distilled water in an optically

perfect cell. The zero adjustment must be performed separately for
each cell type.
sured variable, Concentration is selected.
3 Press the <CAL/ZERO> key.
The menu for adjustment measurements opens up.
46 ba75491d04 07/2006
Photometry \ Adjustment
Zero adjust.
Measure blank value
Delete blank value
4 Using <▲> <▼> and <START/ENTER>, select and start the

Zero adjust. function.
The menu-guided zero adjustment starts.
Zero adjust.
i Insert zero
cell (dist. water)
Cell 16 mm
Start measurement
5 Insert the cell (see section 4.2)

6 Set another cell with <▲> <▼> and <START/ENTER> as nec-
essary.
7 Start the measurement of the zero adjustment with <START/
ENTER>.
After measuring, the result of the zero adjustment is displayed
and stored.
i Zero adjust. successful! (successful zero adjustment) or

i Calibration error! (zero adjustment not successful)
is displayed as the result.
The zero adjustment is completed.
Note
If i Calibration error! was displayed as the calibration result, a note au-
tomatically reminds you of another zero adjustment before the next
measurement.
Measuring is not possible without a valid zero adjustment.
8 Confirm the result with <START/ENTER>.

The zero adjustment is completed.
The meter is ready to measure.
ba75491d04 07/2006 47
4.5.7 Programs
Displaying program You can view the most important data of all methods.
data The method data is ordered according to the program number.
1 Open the Configuration / Photometry / Programs menu.

The display shows the most important data of the selected pro-
gram.
Programs
1: A5/25 MC Program number, test, code
0.20 - 8.00 mg/l
Measuring range with unit
NH4-N
16 mm Citation form
i Scroll with ▲ ▼ Diameter of the cell
Note
This data is also to be found in the photometry analysis manual in the
overview of the test sets and in the individual analysis specifications for
the test sets.
Updating programs Under http://www.WTW.com on the Internet, you can always find the
latest software version with the newest programs and method data for
your pHotoFlex (Turb) (see APPENDIX: FIRMWARE UPDATE).
User-defined programs User-defined programs (methods) can be stored under program num-
bers between 900 and 999. You can store up to 100 user-defined pro-
grams (see section 4.13).
48 ba75491d04 07/2006
4.5.8 Analysis timer
Measuring according to analysis specifications often means there are

waiting periods between the individual steps.
These waiting periods (time intervals) are stored in the instrument with
the program data for each program. The active Analysis timer function
automatically reminds you to observe these time intervals by means of
the menu guidance.
If you want to manually enter time intervals, use the Timer function (see
section 4.5.9).
The Analysis timer with the required time interval is automatically dis-
played at the due point.
Start the Analysis timer with the <START/ENTER> key.
It is not possible to shorten the time intervall.
An acoustic signal sounds when the adjusted time interval has expired.
The Analysis timer function is switched on or off in the Configuration /

Photometry/Analysis timer menu.
This setting generally applies to all measurements with methods ac-
cording to analysis specification.
4.5.9 Timer
When measuring according to analysis specifications, waiting periods

often have to be kept between individual steps of the method.
With the Timer function you manually set a time interval.
If you want to be automatically reminded of the given time interval, use

the Analysis timer function (see section 4.5.8).
The timer is displayed in the measured value display. It always displays

the remaining time of the adjusted time interval.
When the adjusted time interval has expired, the timer indicates
00:00:00 and an acoustic signal sounds.
The Timer function is started in the Configuration / Timer menu by en-

tering a time interval.
ba75491d04 07/2006 49
4.5.10 Measuring diluted samples
If the concentration of a test sample exceeds the measuring range of a

method, you can dilute the sample by a factor 1 ... 99 so that the con-
centration of the diluted test sample is within the measuring range of
the method (see photometry analysis manual). Thus a valid measure-
ment is possible.
After entering the factor for the dilution the meter converts the concen-
tration to that of the undiluted sample.
The display then indicates the measured value of the undiluted sample.
Entering the factor of 1 Select the program for which a dilution factor is to be entered.
the dilution
2 Open the Configuration / Photometry / Dilution menu.
The current factor of the dilution is displayed.
Dilution
Water + sample 0+1
i PROG 1
3 Open the display for the entry of numerals with <START/EN-

TER>.
4 Enter the factor of the dilution with the number keys.
The factor has to be a whole number between 0 ... 99.
5 Confirm the factor with <START/ENTER>.
6 Exit the Dilution menu with <ESC>.
For the following measurements with the selected program, the
concentration of the undiluted sampled is displayed as the
measurement result.
The entered dilution factor is only valid for the selected program. The
dilution factor is erased if:
! the photometer is switched off
! a different program number is selected
! the factor 0 is entered in the Dilution menu.
If a dilution factor is active, it is indicated on the display during measure-

ment in the form [x + 1].
50 ba75491d04 07/2006
4.6 pH value / ORP voltage
You can measure the following variables:

! pH value [ ]
! ORP [mV]
Caution
When connecting an earthed PC/printer, measurements cannot be
performed in earthed media as incorrect values would result. The
RS232 interface is not galvanically isolated.
Temperature For reproducible pH measurements, it is essential to measure the tem-

measurement perature of the test sample.
You have the following possibilities for measuring the temperature:
! Automatic measurement of the temperature by a temperature sen-
sor (NTC30 or Pt1000) integrated in electrode.
! Manual determination and input of the temperature.
The meter recognizes whether a suitable electrode is connected and

automatically switches on the temperature measurement.
The display of the temperature indicates the active temperature mea-

suring mode:
Temperature Resolution of the Temperature of the test

sensor temp. display sample
yes 0.1 °C automatic measurement
- 1 °C manual measurement
and entry
Preparatory activities Perform the following preparatory activities when you want to measure:
1 Connect a pH or ORP electrode to the meter.

2 Press the <M> key (long pressure) repeatedly until the pH &
ORP measuring mode is selected.
sured parameter, pH or ORP is selected.
4 Adjust the temperature of the solutions and measure the cur-
rent temperature if the measurement is made without a temper-
ature sensor.
5 Calibrate or check the meter with the electrode.
ba75491d04 07/2006 51
4.6.2 Measuring the pH value
1 Perform the preparatory activities according to section 4.6.1.

2 Immerse the pH electrode in the test sample.
pH & ORP \ pH
6.94
25.0 °C
[AR]
01.02.05 15:12
sured variable, pH is selected.
AutoRead The AutoRead function (drift control) continually checks the stability of
(Drift control) the measurement signal. The stability has a considerable impact on the
reproducibility of measured values. The display of the measured pa-
rameter flashes until a stable measured value is available.
Criteria With identical measurement conditions, the following applies:
Measured Reproducibility Response time

parameter
pH value Better than 0.01 > 30 seconds
52 ba75491d04 07/2006
4.6.3 Measuring the ORP voltage
Note
ORP electrodes are not calibrated. However, you can check ORP elec-
trodes using a test solution.
1 Perform the preparatory activities according to section 4.6.1.

2 Submerse the ORP electrode in the sample.
pH & ORP \ ORP
157 mV
25.0 °C
[AR]
01.02.05 15:12
sured parameter, ORP is selected.
AutoRead The AutoRead function (drift control) continually checks the stability of
(drift control) the measurement signal. The stability has a considerable impact on the
reproducibility of measured values. The display of the measured pa-
rameter flashes until a stable measured value is available.
Criteria With identical measurement conditions, the following applies:
Measured Reproducibility Response time

parameter
ORP voltage better than 1 mV > 30 seconds
4.6.4 Settings for pH and ORP measurements
Overview For pH and ORP measurements, the following settings are available in
the Configuration / pH & ORP menu:
! Measured parameter
! Calibration record (display, print)
! Selecting the calibration type
! Entering the Calibration interval
! Selecting the Unit for slope
! Selecting the Temperature unit
! Reset
ba75491d04 07/2006 53
Settings/functions The settings can be found in the Configuration / pH & ORP menu.
Menu item Possible Description

setting
Measured parameter pH & ORP
mV
Calibration / - Displays the calibration
Calibration record record of the last calibra-
tion.
Calibration / TEC Buffer sets to be used for
Cal. type NIST/DIN pH calibration.
For details, see
section 4.6.5.
Calibration / 1 ... 999 d Calibration interval for the
Calibration interval pH electrode (in days).
The meter reminds you to
calibrate regularly by the
flashing sensor symbol in
the measured value dis-
play.
Calibration / mV/pH Unit of the slope.
Unit for slope % The % display refers to the
Nernst slope of
-59.16 mV/pH (100 x de-
termined slope/Nernst
slope).
Man. temperature -20 ... +130 °C Entry of the manually de-
termined temperature. For
measurements without
temperature sensor only.
Temperature unit °C, °F Degrees Celsius
Degrees Fahrenheit
Reset Reset all settings for the
pH & ORP measuring
mode (see section 4.10.3)
54 ba75491d04 07/2006
4.6.5 Calibration
Why calibrate? pH electrodes age. This changes the asymmetry (zero point) and slope
of the pH electrode. As a result, an inexact measured value is dis-
played. Calibration determines the current values of the asymmetry
and slope of the electrode and stores them in the meter. Thus, you
should calibrate at regular intervals.
When to calibrate? ! After connecting another electrode

! When the sensor symbol flashes:
– after the calibration interval has expired
– after voltage interruption (e.g. empty batteries, empty
accumulator pack)
Buffer sets for You can use the buffer sets quoted in the table for an automatic calibra-
calibration tion. The pH values are valid for the specified temperature values. The
temperature dependence of the pH values is taken into account during
calibration.
Buffer set Name on the pH values at

display 25 °C
Technical buffer solutions TEC 2.00
4.01
7.00
10.01
NIST/DIN buffer solutions NIST/DIN 1.679
4.006
6.865
9.180
12.454
Note
The buffers are selected in the Configuration / pH & ORP / Cal. type
menu, see section 4.6.4).
ba75491d04 07/2006 55
Calibration points Calibration can be performed using one, two or three buffer solutions in
any order (single-point-, two-point or three-point calibration). The meter
determines the following values and calculates the calibration line as
follows:
Determined val- Displayed calibration data

ues
1-point Asy ! Asymmetry = Asy
! Slope = Nernst slope
(-59.16 mV/pH at 25 °C)
Slp. ! Slope = Slp.
Slp. ! Slope = Slp.
The calibration line is calculated
by linear regression.
Note
You can display the slope in the unit, mV/pH or % (see section 4.6.4).
AutoRead The calibration procedure automatically activates the AutoRead func-

tion.
The current AutoRead measurement can be terminated at any time
(accepting the current value).
Calibration record When finishing a calibration, the new calibration values are displayed
as an informative message (i symbol) first. Then you can decide
whether you want to take over these values of the new calibration or
whether you want to continue measuring with the old calibration data.
After accepting the new calibration values the calibration record is dis-
played.
Displaying and down- You can view the data of the last calibration on the display. Subse-
loading calibration data quently, you can download the displayed calibration data to the inter-
to interface face, e. g. to a printer or PC, with the <PRT> key.
The calibration record of the last calibration can be found under the
Configuration / pH & ORP / Calibration / Calibration record menu item.
56 ba75491d04 07/2006
Sample printout of a
31.10.03 16:13
record pHotoFlex Ser. no. 12345678
Calibration pH & ORP
Calibration date 31.10.03 16:13:33
Calibration interval 7 d
AutoCal TEC
Buffer 1 4.01
Buffer 2 7.00
Buffer 3 10.01
Voltage 1 184.0 mV 24.0 °C
Voltage 2 3.0 mV 24.0 °C
Voltage 3 -177.0 mV 24.0 °C
Slope -60.2 mV/pH
Asymmetry 4.0 mV
Sensor +++
Calibration evaluation After calibrating, the meter automatically evaluates the calibration. The
asymmetry and slope are evaluated separately. The worse evaluation
of both is taken into account. The evaluation appears on the display
and in the calibration record.
Sample dis- Calibration Asymmetry Slope

play record [mV] [mV/pH]
+++ -15 ... +15 -60.5 ... -58
++ -20 ... +20 -58 ... -57
+ -25 ... +25 -61 ... -60.5

or
-57 ... -56
- -30 ... +30 -62 ... -61
or
-56 ... -50
Clean the electrode according
to the electrode operating
manual
< -30 or ... -62 or
---- ----
> 30 ... -50
Eliminate the error according to
chapter 6 WHAT TO DO IF...
ba75491d04 07/2006 57
Preparatory activities Perform the following preparatory activities when you want to calibrate:
1 Connect the pH electrode to the meter.

The pH measured value display is displayed on the screen.
2 Keep the buffer solutions ready. Adjust the temperature of the
buffer solutions, or measure the current temperature if you
measure without a temperature sensor.
4.6.6 Carrying out the TEC and NIST/DIN calibration procedures
The two calibration procedures only differ in the usage of different buff-
er sets (see section 4.6.5). Make sure the Cal. type is correctly set in
the pH & ORP / Calibration menu (see section 4.6.4).
For this procedure, use any one, two or three WTW technical buffer so-
lutions in ascending or descending order.
The TEC calibration is described below. With the NIST/DIN calibration,

the NIST/DIN buffer recognition and different nominal buffer values are
displayed. Apart from that, the procedure is identical.
Note
The TEC calibration for pH 10.01 is optimized for the WTW technical
buffer solution TEP 10 Trace or TPL 10 Trace. Other buffer solutions
can lead to an erroneous calibration. The correct buffer solutions are
given in the WTW catalog or on the Internet.
sured parameter, pH or ORP is selected.
2 Start the calibration with <CAL/ZERO>.
The calibration display appears.
pH & ORP \ Calibration

i Buffer recognition TEC
i Immerse sensor in buffer 1
Continue
3 Immerse the electrode in buffer solution 1.

4 If the Set temperature menu item appears, measure and enter
the temperature of the buffer manually (measurement without
temperature sensor).
58 ba75491d04 07/2006
5 Using <▲> <▼>, select Continue and press <START/EN-

TER>. The buffer is measured.
The measured value is checked for stability (AutoRead).

i Buffer value = 7.00
i U = 3 mV
i Temperature = 24.8 °C
Terminate AutoRead
6 Wait for the end of the AutoRead measurement or accept the

calibration value with <START/ENTER>.
The calibration display for the next buffer appears.

Exit with one point calibration

Continue
7 For single-point calibration, select Exit with one point calibra-

tion with <▲> <▼> and confirm with <START/ENTER>.
The calibration is completed as a single-point calibration.
The new calibration values are displayed as an informative
message (i).
You have the following options:
! Accept the new calibration values with <START/ENTER>.
Subsequently, the calibration record is displayed and output
to the interface at the same time.
! To switch to the measured value display without accepting
the new calibration values, press <M> (short pressure) or
<ESC>.
Note
For single-point calibration, the instrument uses the Nernst slope
(-59.16 mV/pH at 25 °C) and determines the asymmetry of the elec-
trode.
ba75491d04 07/2006 59
Continuing for two-point

8 Thoroughly rinse the electrode with distilled water.
calibration
(Cal. type TEC) 9 Immerse the electrode in buffer solution 2.
10 If the Set temperature menu item appears, measure and enter
the temperature of the buffer manually (measurement without
temperature sensor).

i U = -177 mV
Terminate AutoRead
12 Wait for the end of the AutoRead measurement or Terminate

AutoRead with <START/ENTER> and take over the calibration
value.
The calibration display for the next buffer appears.

Exit with 2 point calibration

Continue
13 For two-point calibration, select Exit with 2 point calibration with

<▲> <▼> and confirm with <START/ENTER>.
The calibration is completed as a two-point calibration.
message (i).
<ESC>.
60 ba75491d04 07/2006
Continuing for three-

14 Thoroughly rinse the electrode with distilled water.
point calibration
(Cal. type TEC) 15 Immerse the electrode in buffer solution 3.
16 If necessary, measure the temperature of buffer 3 manually,
then enter and confirm it with <▲> <▼> and <START/ENTER>
in the Set temperature setting.

i U = 184 mV
Terminate AutoRead
18 Wait for the end of the AutoRead measurement or Terminate

AutoRead with <START/ENTER> and take over the calibration
value.
message (i).
<ESC>.
ba75491d04 07/2006 61
4.7 Turbidity
Venting the sample Air bubbles in the sample affect the measuring result to a massive ex-
tent because they have a large scattering effect on the incident light.
Larger air bubbles cause sudden changes in the measured values
whereas smaller air bubbles are recorded by the instrument as turbidi-
ty. Therefore, avoid or remove air bubbles:
Avoiding or removing ! During sampling, ensure all movement is kept to a minimum
air bubbles ! If necessary, vent the sample (ultrasonic baths, heating or adding a
surface-active substance to reduce the surface tension)
4.7.2 Aligning and marking a cell
Even completely clean quality cells exhibit tiny directional differences

in their light transmittance. Therefore, if you want to achieve accurate
and reproducible measurement results, it is necessary to always align
the sample cells and cells for calibration standards in the same way
(see section 2130 of the "Standard Methods for the Examination of Wa-
ter and Wastewater", 19th edition).
To do so, the optimum alignment of the cell is determined.
Aligning the cell 1 Press the <M> key (long pressure) repeatedly until the Turbid-
ity measuring mode is selected.
4 Align the cell:
! Press and hold the <START/ENTER> key.
! Slowly and in small steps turn the cell by one complete ro-
tation (by 360 °).
After each step wait for a short time until the displayed mea-
sured value is stable.
! Turn the cell back to the position with the lowest measured
value.
Note
To keep the drift as low as possible, the time for aligning the cell while
pressing and holding the <START/ENTER> key is limited to 30 sec-
onds. After this time, the meter starts measuring automatically.
5 Release the <START/ENTER> key.

Measurement starts. The measured value is displayed.
Marking a cell To be able to quickly bring a cell into the optimum position, it is helpful
62 ba75491d04 07/2006
to mark the optimum position of the cell once it is determined. This

shortens each measurement or calibration procedure with this cell con-
siderably.
The marking can, e. g., be done on a label on the cap of the cell.
6 Mark the optimum position of the cell.

The cell is prepared for the shortened measuring and calibra-
tion procedures.
4.7.3 Measuring turbidity
Caution
Never pour any liquids directly into the cell shaft. Always use a
cell for measurement. The meter only measures precisely if the
cell is closed with the black light protection cap (WTW cells).
Note
The outside of the cell always has to be clean, dry, and free of finger-
prints and scratches. Clean the cell before starting to measure (see
section 5.2.2). Only hold the cells by the top or by the black light pro-
tection cap.
Measuring 1 Press the <M> key (long pressure) repeatedly until the Turbid-
2 Rinse out a clean cell with the sample to be measured:
Pour approximately 10 ml sample into the cell. Close the cell
and rotate it several times before throwing the sample away.
3 Repeat the rinsing procedure twice more.
4 Fill the cell with the sample to be measured (approx. 15 ml).
Close the cell with the black light protection cap.
ba75491d04 07/2006 63
7 Align the cell:

! Marked cell
– Align the marking on the cell cap with the marking on the
cell shaft.
– Press and for a short time hold the <START/ENTER>
key until the measured value is displayed.
! Unmarked cell (see page 62)
– Press and hold the <START/ENTER> key.
– Slowly and in small steps turn the cell by one complete
rotation (by 360 °). After each step wait for a short time
until the displayed measured value is stable.
– Turn the cell back to the position with the lowest mea-
sured value.
Note
To keep the drift as low as possible, the time for aligning the cell while
pressing and holding the <START/ENTER> key is limited to 30 sec-
onds. After this time, the meter automatically starts measuring or cali-
brating.

Measurement starts. The measured value is displayed.
Turbidity
FNU
157.0 NTU
01.02.05 15:12
9 Repeat the steps 2 to 8 for further samples.
Display if the If the measured value is outside the measuring range of the
measuring range is pHotoFlex Turb, it is indicated on the display:
exceeded
Turbidity
FNU
> 1100 NTU
01.02.05 15:12
64 ba75491d04 07/2006
4.7.4 Calibration
When to calibrate? ! Regularly every 90 days

! With a temperature change
Calibration procedures For the menu-guided three-point calibration you need the following
and calibration three calibration standards in the mentioned order:
standards
Standard no. NTU/FNU
1 1000
2 10,0
3 0,02
Preparing calibration Perform the following preparatory activities when you want to calibrate:
1 Keep the cells with the required calibration standards ready

and mark them as necessary (see page 62).
Caution
Never open the cells with the calibration standards.
Carrying out calibration 1 Press the <M> key (long pressure) repeatedly until the Turbid-
2 Press the <CAL/ZERO> key.
The menu-guided calibration begins.
Turbidity \ Calibration
i Insert standard
1000 FNU/NTU
i Press and hold <START>
i Align sample
3 Insert the cell with the displayed calibration standard (here e.g.
1000 NTU/FNU) in the cell shaft (see section 4.2).
ba75491d04 07/2006 65
4 Align the cell:

! Marked cell:
– Align the marking on the cell cap with the marking on the
cell shaft.
– Press and time hold the <START/ENTER> key until the
measured value is displayed.
! Unmarked cell (see page 62)
– Press and hold the <START/ENTER> key.
– Slowly and in small steps turn the cell by one complete
rotation (by 360 °).
– After each step wait for a short time until the displayed
measured value is stable.
– Turn the cell back to the position with the lowest mea-
sured value.
Turbidity \ Calibration
i Turb. = 1000 FNU/NTU
i Start calibration
by releasing <START>

Measurement of the calibration standard begins.
Note
Before measuring the third calibration standard of 0.02 NTU/FNU you
can exit the calibration with <ESC> at any time.
The new calibration data is discarded. The old calibration data is further
used.
6 Repeat the steps 4 - 6 with the calibration standards, 10.0 NTU/

FNU and 0.02 NTU/FNU.
After measuring the 0.02 NTU/FNU calibration standard, the
calibration result (i Calibration successful! or i Calibration er-
ror!) is displayed and stored.
Calibration is completed.
7 Confirm the calibration result with <START/ENTER>.
The display shows instructions for the first measurement.
66 ba75491d04 07/2006
Turbidity
i Insert sample
i Press and hold <START>
i Align sample
Note
If i Calibration error! was displayed as the calibration result, a note ap-
pears on the display to recalibrate before measuring.
Should a valid calibration not be possible the meter also offers to con-
tinue measuring with the last valid calibration data.
ba75491d04 07/2006 67
4.8 Storing
The meter has 1000 storage locations for measurement datsets.
You can transmit measured values (datasets) to the data storage with
the <STO> key.
Each storage process transmits the current dataset to the interface at
the same time.
The number of storage locations that are still free is displayed in the
Store menu. The number of storage locations that are occupied is dis-
played in the System \ Measured value memory menu.
Measurement dataset A complete dataset consists of:

! Date/time
! ID number (ID)
! and the following measurement data depending on the selected
measuring mode
Measuring Measurement data

mode
Photometry: ! Program number
! Measured value
! Citation form
! Use of a blank value (BV)
! Dilution (x +1)
Turbidity: ! Measured value
pH & ORP: ! Measured value (pH/mV)
! Measured temperature value (°C/°F)
! AutoRead info (AR)
AR appears with the measured value if the Auto-
Read criterion was met while storing (stable mea-
sured value). Otherwise, the AR display is missing.
68 ba75491d04 07/2006
4.8.1 Storing measurement datsets
Proceed as follows to transmit to the data storage and simultaneously

output to the interface a measurement dataset:
1 Press the <STO> key.

The Store display appears.
Store (996 free)s

i 02.02.2005 11:24:16
0.00 mg/l PO4-P BV
PROG 1
ID: 1
Store (ID: 1:
2 Using <▲> <▼>, <START/ENTER> and the number keys,

change and confirm the ID number (ID) as necessary (0 ...
999).
3 Using <START/ENTER> or <STO>, confirm Store.
The dataset is stored. The instrument switches to the mea-
sured value display.
Note
A measurement dataset is stored quickly by twice pressing <STO>. It
is stored with the ID last set.
If the storage is full You can erase the entire storage (see section 4.8.5), or overwrite the
oldest dataset with the next storing procedure.
A security prompt appears before a dataset is overwritten.
ba75491d04 07/2006 69
4.8.2 Filtering measurement datsets
The functions to display and download stored measurement datsets

(see section 4.4.1) refer to all stored measurement datsets that corre-
spond to the adjusted filter criteria.
The settings can be found in the Configuration / System / Measured val-

ue memory /Data filter menu.
Data filter Menu item Setting/function Description

Filter Filter criteria:
No filter Data filter switched off
ID Selection according to ID
number
PROG Selection according to pro-
gram
Date Selection according to pe-
riod
ID + PROG Selection according to pro-
gram and ID number
ID + Date Selection according to pe-
riod and ID number
PROG + Date Selection according to pro-
gram and date
ID + PROG + Date Selection according to ID,
program and date
ID Entry of filter criteria
PROG These menu items are
made visible by selecting
Date the filter criteria in the Filter
menu.
70 ba75491d04 07/2006
4.8.3 Displaying measurement datsets
You can read out stored datasets on the display. Only those datasets
are displayed that correspond to the selected filter criteria (see
section 4.8.2).
Start reading out the data on the display in the menu, Configuration /
System / Measured value memory / Display.
Display of a dataset
02.02.2005 11:24:16
ID: 1
7.000 (pH) 24.8 °C AR
i Scroll with ▲ ▼
Further datasets that correspond to the filter criteria are displayed with
the <▲> <▼> keys.
Quitting the display To quit the display of stored measurement datasets, you have the fol-
lowing options:
! Switch directly to the measured value display with <M> (short pres-
sure).
! Leave the display and switch to the higher menu with <ESC> or
<START/ENTER>.
4.8.4 Downloading the measurement datsets to the RS232

interface
You can download stored datasets to the RS232 interface. Only those
datasets are downloaded that correspond to the selected filter criteria
The datasets are downloaded in the adjusted output format (see

section 4.9.3).
The data download to the interface is started in the menu, Configura-

tion / System / Measured value memory / RS232 download.
ba75491d04 07/2006 71
4.8.5 Erasing stored measurement datasets
You can erase the stored measurement datsets altogether if you do no

longer need them.
Erasing all measurement datsets is done in the menu, Configuration /

System / Measured value memory / Delete.
Note
Erasing individual datasets is not possible. If all storage locations are
occupied, however, it is possible to overwrite the oldest dataset at a
time. A security prompt appears before a dataset is overwritten.
4.9 Transmitting data (RS 232 interface)

Via the RS 232 interface, you can transmit data to a PC or an external
printer.
4.9.1 Connecting a PC/external printer
Connect the interface to the devices via the AK540/B (PC) or AK540/S
(external printer) cable.
Caution
The RS232 interface is not galvanically isolated.
When connecting an earthed PC/printer, measurements cannot be
performed in earthed media as incorrect values would result.
Set up the following transmission data on the PC/printer:
Baud rate can be selected from:

1200, 2400, 4800, 9600, 19200
The baud rate must agree with the baud rate set on
the PC/printer.
Handshake RTS/CTS
PC only:
Parity none
Data bits 8
Stop bits 1s
72 ba75491d04 07/2006
Socket assignment 3 1 RTS

4 2 2 RxD
6
3 TxD
5 1 4 DTR
5 SG
RS 232 6 CTS
REC
4.9.2 Configuring the RS232 interface
For an error-free data transmission, the RS232 interface should be set

to the same transmission speed (Baud rate) on the pHotoFlex (Turb)
and PC/printer.
You can set the following values for the baud rate on the
pHotoFlex (Turb) : 1200, 2400, 4800, 9600, 19200.
The baud rate is selected in the menu, Configuration / System / Inter-

face / Baud rate.
4.9.3 Selecting the output format of datasets
For downloading data to the interface you can select the output format.
It is selected in the menu, Configuration / System / Interface / Output

format.
The ASCII output format delivers formatted datasets.

The CSV output format delivers datasets separated by ";".
Output format, ASCII

pHotoFlex Ser. no. 12345678
31.10.04 09:56:20
ID: 1
10.01 (pH) AR
25 °C
________________________________________
pHotoFlex Ser. no. 12345678

31.10.04 15:48:08
ID 1 / PROG 2
1.1 mg/l Pb [BV][9 + 1]
________________________________________
etc...
ba75491d04 07/2006 73
Output format, CSV 1 2 3 4 5 6 7 8 9 10
15.01.05;15:06:49;1;8;O2;[BW];[9 + 1];6.5;mmol/l;VALID;
15.01.05;15:09:52;0;;;[AR];;7.00;<pH>;VALID;25;°C;VALID
11 12 13
Data Description
1 Date Date of storing
2 Time Time of storing
3 ID adjusted ID
4 Program number only for the measured parameter,
Concentration
5 Citation form only for the measured parameter,
Concentration
6 AR/ ! AutoRead (measuring mode, pH & ORP)
BV ! Blank value (measured parameter,
Concentration)
7 Dilution only for the measured parameter,
Concentration
8 Measured value ! Measured value or
! Upper/lower measuring range limit
(only with measured value status, OFL/
UFL)
9 Unit of 8 ! Unit of the measured value or
! Designation of dimensionless measured
values, e.g. <pH>
10 Measured value ! VALID: Measured value valid
status of 8 ! INVALID: Measured value invalid
! UFL: Measured value below the lower
measuring range limit
! OFL: Measured value above the upper
measuring range limit
11 Temperature val- ! Temperature (measuring mode, pH &
ue / wavelength ORP)
of the measure- ! Wavelength (measured parameter,
ment Absorbance / % Transmission)
74 ba75491d04 07/2006
Data Description
12 Unit of 11 ! °C / °F
! nm
13 Measured value VALID, INVALID, UFL, OFL
status of 11
4.9.4 Transmitting data
The following table shows which data are transmitted to the interface in
which way:
Data Operation / description

Current ! Press <PRT>.
measured value ! Simultaneously with every manual storage
process.
Stored measured ! Display stored dataset and press <PRT>.
values ! All datasets according to the filter criteria via
the RS232 download function
Calibration record ! Calibration record of a pH electrode with
(pH electrode) <PRT> (after calling up from the storage or at
the end of a calibration).
! Calibration record of a pH electrode at the
end of a calibration procedure is printed au-
tomatically.
Note
With the <PRT> key you output the data that is being shown on the dis-
play to the interface (displayed measured values, stored measurement
datsets, calibration record).
ba75491d04 07/2006 75
4.10 Reset
You can reset (initialize) all system and measurement settings.
Note
For turbidity measurement, there are no resettable settings.
4.10.1 Resetting the system settings
With the System / Reset function, all resettable settings are reset.
! Settings for pH & ORP (see section 4.10.3)

! Settings for Photometry (see section 4.10.2)
! System settings
System setting Default settings

Baud rate 4800 Baud
Output format ASCII
Illumination Auto off
Contrast 50 %
Brightness 50 %
Switchoff time 30 min
Beep On
Measuring mode Photometry
76 ba75491d04 07/2006
4.10.2 Resetting the photometer settings
With the Photometry / Reset function, all photometer settings are reset.
Setting Default settings

PROG 0
Cell size 16 mm
Measured parameter Konzentration
Wavelength 436 nm
Blank values all erased
4.10.3 Resetting the pH settings
Note
The calibration data are reset to the default settings together with the
measuring parameters. Recalibrate after performing a reset.
The following settings for pH measurements are reset to the default

settings with the Reset function:
Setting Default settings

Cal. type TEC
Calibration interval 7d
Measured parameter pH & ORP
Asymmetry (Asy) 0 mV
Slope (Slp.) -59.16 mV/pH
Temperature, manual 25 °C
Temperature °C
ba75491d04 07/2006 77
4.11 Meter information

The following meter information is listed in the Configuration / Info
menu:
! Model designation
! Software version
! Version number(s) of the stored program data
! Series number of the meter
Info
i Model: pHotoFlex
i Software: V 0.35
i Prog.: V 0.07
i Ser. no.: 04280003
4.12 Software update

With a software update you obtain the current software with all new pro-
grams and method data (see appendix).
A software update comprises
! new instrument software

! new programs (methods)
! revisions of existing methods
The current software version can be found on the Internet under

www.WTW.com.
The proceeding for updating the software can be found in the appendix
(see APPENDIX: FIRMWARE UPDATE).
78 ba75491d04 07/2006
4.13 Administrating user-defined methods

User-defined programs can be
! entered
! read out
! erased.
To store user-defined programs (methods) in the meter, determine a

calibration line for your method yourself and transmit the data of this
calibration line to the pHotoFlex (Turb).
Administration of the method data can be carried out in the LSdata soft-
ware (see operating manual LSdata) or with the aid of a terminal pro-
gram (section 4.13.1).
You need the following data in any case:
Data Possible entries/examples

Program No: 900 ... 999
Model: Any name
(max. 5 characters)
Cuvette [mm]: 16 or 28
Wave length [nm]: 436, 517, 557, 594, 610 or 690
MRB: e.g. 0.05
(measuring range beginning)
MRE: e.g. 8.00
(measuring range end)
E0: (Offset) e.g. 0.0
Slope: e.g. 1.0
Resolution: 0.0001, 0.001, 0.01, 0.1, 1, 2, 5, 10
or 100
Formula: (citation form) e.g. PO4-P (max. 9 characters)
Unit e.g. mg/l (max. 7 characters)
ba75491d04 07/2006 79
4.13.1 Administrating user-defined programs with a terminal

program
Generally, a terminal program serves to establish a connection to a de-

vice on a data interface and to communicate with the device via a con-
sole on the display.
Thus, it is also possible to send command lines.
Terminal programs are available for different operating systems by dif-
ferent manufacturers. Windows (version 95 to XP) contains the "Hyper-
Terminal" terminal program. It is in the program menu under
accessories.
For more detailed information please refer to the user information of the
terminal program.
Note
The CD-ROM contains a configuration file for the HyperTerminal pro-
gram. By double-clicking the configuration file (*.ht), the HyperTerminal
with the required pre-settings for data exchange with the meter is
opened.
The pHotoFlex (Turb) can administrate the database of user-defined
programs via command lines.
1 With the aid of the AK 540/B interface cable, connect the

pHotoFlex (Turb) 540 to the serial interface (COM port) of the
PC (see section 8.1.1).
2 Make sure the pHotoFlex (Turb) is switched on.
3 Start the terminal program on the PC.
4 Configure the connection settings for the COM interface as
necessary.
Storing user-defined Enter a command line according to the following scheme in the terminal
programs program:
U.500#11,Program No,Model,Cuvette [mm],Wave length [nm],MRB,
MRE,E0,Slope,Resolution,Formula,Unit
Example:
U.500#11,900,Test,16,436,0.0,2.0,0.0,1.0,0.01,test,
mg/l
The individual data sections of the command line are separated by

commas. The dot "." has to be used as a decimal separator within a
data section.
5 Enter the command line.
80 ba75491d04 07/2006
6 Finish the command line with Enter.

The data is transmitted to the pHotoFlex (Turb).
Note
If a program is already stored under the selected number, the program-
ming procedure is canceled. To store the program under the selected
number, first erase the program stored under the number.
After successful transmission, the terminal program writes "!>".

If the transmission failed, the terminal program writes "!>".
Erasing user-defined To erase user-defined programs, enter a command line according to

programs the following scheme in the terminal program:
Erase all user-defined Erase one user-

programs defined program
Command line U.520 U.521#1,Program No
Example U.520 U.521#1,900

The requested data is displayed on the terminal as the result.
After successful transmission, the requested data is displayed on the

terminal.
Reading out user- To read out user-defined programs, enter a command line according to
defined programs the following scheme in the terminal program:
Read out all user- Read out one user-

defined programs defined program
Command line U.510 U.511#1,Program No
Example U.510 U.511#1,900
ba75491d04 07/2006 81

The requested data is displayed on the terminal as the result.
After successful transmission, the terminal program writes "!>".

82 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Maintenance, cleaning, disposal
5 Maintenance, cleaning, disposal

5.1 Maintenance
The meter is almost maintenance-free.
The only maintenance task is replacing the batteries or accumulator
pack.
5.1.1 Inserting/exchanging the batteries
Caution
Make sure that the poles of the batteries are the right way round.
The ± signs on the batteries must correspond to the ± signs in the
battery compartment.
1 Open the battery compartment:

– Unscrew the two screws (1) on the underside of the meter,
– Remove the lid of the battery compartment (2).
2 If necessary, take four old batteries out of the battery compart-
ment.
3 Insert four batteries (3) in the battery compartment.
4 Close the battery compartment and fix it with the screws.
ba75491d04 07/2006 83
Maintenance, cleaning, disposal pHotoFlex / pHotoFlex Turb
5.1.2 Retrofitting the accumulator pack
Caution
Use original WTW accumulator packs only.
Together with the power pack the accumulator pack is available as an

accessory (see section 8.1).
1 Open the battery compartment:

– Unscrew the two screws (1) on the underside of the meter,
– Remove the lid of the battery compartment (2).
2 If necessary, take four old batteries out of the battery
compartment.
3 Connect the cable of the accumulator pack with the socket (3)
on the bottom of the battery compartment and insert the accu-
mulator pack in the battery compartment.
4 Close the battery compartment and fix it with the screws.
84 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Maintenance, cleaning, disposal
5.2 Cleaning
Occasionally wipe the outside of the meter with a damp, lint-free cloth.
Disinfect the housing with isopropanol as required.
Caution
The housing components are made out of synthetic materials
(polyurethane, ABS and PMMA). Thus, avoid contact with acetone
and similar detergents that contain solvents. Remove any splash-
es immediately.
5.2.1 Cleaning the cell shaft
If liquid is in the cell shaft (e.g. due to a spilled cell), clean the cell shaft
as follows:
Caution
Cells can contain poisonous or corrosive substances. If the con-
tent is released follow the danger warnings on the cell. If neces-
sary, take corresponding protective measures (protective
goggles, protective gloves etc.).
1 Switch the pHotoFlex (Turb) off and pull out the power plug.
2 Rinse the cell shaft with distilled water.
5.2.2 Cleaning the cells
Cells have to be clean, dry, and free of fingerprints and scratches.

Therefore, clean them regularly:
1 Clean the cells inside and out with hydrochloric acid or labora-
tory soap.
2 Rinse out several times with distilled water.
3 Let them dry in the air.
4 Only hold the cells by the top or by the light protection cap so
that the optical path is not impaired.
5 Before measuring, clean the cell with the enclosed cleaning
cloth.
Note
Scratches in the glass change the optical characteristics of the cell and
falsify the measured value. For this reason, never use scratched cells!
ba75491d04 07/2006 85
Maintenance, cleaning, disposal pHotoFlex / pHotoFlex Turb
5.3 Disposal
Packing This meter is sent out in a protective transport packing.
We recommend: Keep the packing material. The original packing pro-
tects the meter from transport damages.
Batteries/accumulator Remove the batteries or accumulator pack from the meter (see
pack section 5.1).
Dispose of the batteries or accumulator pack at a suitable facility ac-
cording to local legal requirements. It is illegal to dispose of the accu-
mulator pack with household refuse.
NiMH
Meter Dispose of the meter without the batteries and accumulator pack as
electronic waste at an appropriate collection point.
86 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb What to do if...
6 What to do if...
6.1 General errors
Display, LoBat Cause Remedy

– The batteries or accumulator – Insert new batteries
pack are largely depleted – Charge the accumulator pack
(see section 3.2)
Instrument does not Cause Remedy

react to keystroke
– Software error – Processor reset:
– Operating condition undefined Press the <START/ENTER>
or EMC load unallowed and <PRT> key
simultaneously.
RS232 interface Cause Remedy

does not react
– Software error – Processor reset:
– Operating condition undefined Press the <START/ENTER>
or EMC load unallowed and <PRT> key
simultaneously.
Error message, Cause Remedy

Error
0, 8, 16, 16384 – Instrument error – Repeat measurement
– Meter defective,
send meter to WTW for repair
and quote the error number
ba75491d04 07/2006 87
What to do if... pHotoFlex / pHotoFlex Turb
6.2 Photometry
Measuring range Cause Remedy

undercut or exceeded
– Program not suitable – Select program with suitable
measuring range
– Dilute the sample
Obviously incorrect Cause Remedy

measured values
– Measurement disturbed by – Close the external light cover.
external light
– Cell not correctly inserted – Insert the cell so that it is
positioned on the bottom of
the cell shaft.
– Cell contaminated – Clean the cell
– Cell shaft contaminated – Clean the cell shaft
– Dilution set incorrectly – Set the dilution
– Selected program unsuitable – Select other program
– Zero measurement incorrect – Perform zero measurement
– Blank value incorrect – Remeasure the blank value
Measuring range Cause Remedy

undercut or exceeded
Electrode:
– Air bubble in front of the – Remove air bubble
diaphragm
– Air in the diaphragm – Extract air or
moisten diaphragm
– Gel electrolyte dried out – Replace electrode
Test sample
– The pH value lies outside the – not possible
measuring range
88 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb What to do if...
Measured value display Cause Remedy

----
(calibration error) Electrode:
– Diaphragm contaminated – Clean diaphragm
– Membrane contaminated – Clean membrane
– Moisture in the plug – Dry plug
– Not enough electrolyte – Top up electrolyte
– Electrode obsolete – Replace electrode
– Electrode broken – Replace electrode
– Socket damp – Dry socket
Calibration procedure:
– Incorrect solution temperature – Set up correct temperature
(without temperature sensor)
– Incorrect buffer solutions – Select buffer solutions
suitable for the calibration
procedure
– Buffer solutions too old – Use only once.
Note the shelf life
No stable measured Cause Remedy

value
pH electrode:
– Diaphragm contaminated – Clean diaphragm
– Membrane contaminated – Clean membrane
Test sample
– pH value not stable – Measure with air excluded if
necessary
– Temperature not stable – Temper if necessary
Electrode + test sample:
– Conductivity too low – Use suitable electrode
(e.g. in ultrapure water)
– Temperature too high – Use suitable electrode
– Organic liquids – Use suitable electrode
ba75491d04 07/2006 89
What to do if... pHotoFlex / pHotoFlex Turb
Obviously incorrect Cause Remedy

measured values
pH electrode:
– Not connected – Connect electrode
– Cable broken – Replace cable or electrode
– pH electrode unsuitable – Use suitable electrode
– Temperature difference – Adjust temperature of buffer
between buffer and test or sample solutions
sample too high
– Measurement procedure not – Follow special procedure
suitable
Sensor symbol flashes Cause Remedy

– Calibration interval expired – Recalibrate the measuring
system
6.4 Turbidity
Error message Cause Remedy

Measured values
obviously incorrect – Cell not correctly inserted – Lock cell into place
– Cell contaminated – Clean the cell
– Calibration too old – Carry out calibration
Measured value display Cause Remedy

< 0.01 FNU
– Measured value outside the – not possible
measuring range
90 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Technical data
7 Technical data
7.1 General data
7.1.1 pHotoFlex (Turb)
Dimensions approx. 236 x 86 x 117 mm

Weight approx. 0.6 kg (without batteries)
Mechanical structure Type of protection IP 67
Electrical safety Protective class III
Test certificates cETLus, CE, FCC
Storage - 25 °C ... + 65 °C
Ambient conditions Operation 0 °C ... + 50 °C
Climatic class 2
Allowable relative Yearly mean: 75 %

humidity 30 days /year: 95 %
other days:
85%
Power Batteries 4 x 1.5 V, type AA

supply Operating time with approx. 5000 measurements
battery operation
Accumulator pack 5 x 1.2 V nickel metal hydride (NiMH),
(optional) type AA
Power pack FRIWO FW7555M/09, 15.1432.500-00
Charging device Friwo Part. No. 1883259
(optional) Input: 100 ... 240 V ~ / 50 ... 60 Hz / 400 mA
Output: 9 V = / 1,5 A
Connection max. overvoltage category II
Primary plugs contained in the scope of de-
livery: Euro, US, UK and Australian.
ba75491d04 07/2006 91
Technical data pHotoFlex / pHotoFlex Turb
Serial Connection of the cable AK 540/B or AK 540/S

interface Baud rate adjustable:
1200, 2400, 4800, 9600, 19200 Baud
Type RS232
Data bits 8
Stop bits 2
Parity None
Handshake RTS/CTS
Cable length max. 15 m
Guidelines EMC EC guideline 89/336/EEC

and norms used EN 61326-1/A3:2003
FCC Class A
Instrument safety EEC guideline 73/23/EEC
EN 61010-1 :2001
UL3101-1
CAN/CSA-C22.2 No. 1010.1-92
Climatic class VDI/VDE 3540
IP protection EN 60529:1991
FCC Class A Equipment Statement

Note: This equipment has been tested and found to comply with
the limits for a Class A digital device, pursuant to Part 15 of the
FCC Rules. These limits are designed to provide reasonable
protection against harmful interference when the equipment is
operated in a commercial environment. This equipment
generates, uses, and can radiate radio frequency energy and, if
not installed and used in accordance with the instruction manual,
may cause harmful interference to radio communications.
Operation of this equipment in a residential area is likely to cause
harmful interference in which case the user will be required to
correct the interference at his own expense.
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pHotoFlex / pHotoFlex Turb Technical data
7.1.2 LabStation
Dimensions approx. 236 x 82 x 170 mm

Weight approx. 0.6 kg
7.2 Photometry
Optical measuring prin- LED photometer with filter

ciple
Interference filter 436 nm, 517 nm, 557 nm, 594 nm, 610 nm, 690 nm
Accuracy: ± 2 nm
Photometric 0.005 or better
reproducibility
Photometric resolution 0.001
Warm-up time none
Measuring time approx. 2s
Measured parameters Concentration (method dependent, selectable display form),
absorbance, transmission
Measuring range Absorbance: -0.200 ... +2.000
Transmission: 1 ... 150 %
User-defined programs 100
Resolution 1.00 ... 9.99 0,01 %
Transmission
10.0 ... 150 ± 0.1
ba75491d04 07/2006 93
Technical data pHotoFlex / pHotoFlex Turb
Measuring ranges, Variable Measuring range Resolution

resolution pH - 2.00 ... + 16.00 0.01
U [mV] - 1000 ... + 1000 1
T [°C] - 5.0 ... + 100.0 0.1
T [°F] - 23.0 ... + 212.0 0.1
Manual Variable Range Increment

temperature input Tmanual [°C] - 20 ... + 100 1
Accuracy (± 1 digit) Variable Accuracy Temperature of the test

sample
pH * ± 0.01 + 15 °C ... + 35 °C
U [mV] ±1 + 15 °C ... + 35 °C
T [°C] ± 0.3 0 °C ... + 55 °C
T [°F] ± 0.54 0 °C ... + 55 °C
* when measuring in a range of ± 2 pH around a calibration point
7.4 Turbidity
Measuring principle Nephelometric measurement according to DIN EN ISO 7027

Light source Infrared LED
Measuring range 0.01 ... 1100 NTU/FNU
Resolution in the range
0.01 ... 9.99 max 0.01 NTU/FNU
in the range
10.0 ... 99.9 max 0.1 NTU/FNU
in the range
100 ... 1100 max 1 NTU/FNU
Accuracy in the range ± 2% of the measured value
0 ... 1000 NTU/FNU or ± 0.01 NTU/FNU
Measuring time 4 seconds
Calibration Automatic 3-point calibration
94 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Accessories, options
8 Accessories, options
8.1 WTW accessories
Description Model Order no.

LabStation pHotoFlex LS 251 301
Accumulator with pHotoFlex pHotoFlex BB 251 300
power pack
3 empty cuvettes, 28 x 60 mm LKS28-Set 251 302
Calibration standard kit for Kal.Kit Turb 430 IR 600 560
Turb 430 IR/pHotoFlex Turb
Thermoprinter* P3001 250 045
Needle printer* LQ 300+ 250 046
16 mm empty cell RK 14/25 250 621
* a connection cable is required to connect the printer (see

section 8.1.1)
8.1.1 Connection cable:
PC You can connect a PC to the pHotoFlex (Turb) in one of the following

ways:
Description Model Order

no.
! Connection PC - pHotoFlex (Turb)
– Cable AK 540/B 902 842
+ USB adapter Ada USB 902 881
(for USB connection on PC)
! Connection PC - LabStation
– Zero modem cable AK Labor 902 758
+ USB adapter Ada USB 902 881
(for USB connection on PC)
ba75491d04 07/2006 95
Accessories, options pHotoFlex / pHotoFlex Turb
Thermoprinter You can connect the P3001 to the pHotoFlex (Turb) in the following
ways:

no.
! Connection P3001 - pHotoFlex (Turb)
– Cable AK 540/S 902 843
! Connection P3001 - LabStation
– Cable AK 3000 250 745
in conjunction with an adapter Specialist shops
(socket - socket) [GenderChanger]
or:
– Cable, 2 x 9-pin (socket - plug) Specialist shops
Needle printer You can connect an LQ300 needle printer to the pHotoFlex (Turb) in
one of the following ways:

no.
! Connection LQ300 - pHotoFlex (Turb)
– Cable AK 540/B 902 842
with adapter Specialist shops
9-pin (plug) - 25-pin (plug)
! Connection LQ300 - LabStation
– Cable AK/LQ300 250 746
in conjunction with an adapter Specialist shops
(socket - socket) [GenderChanger]
or:
– Zero modem cable, Specialist shops
9-pin (socket) - 25-pin (plug)
96 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Accessories, options
8.2 Optional extensions of the pHotoFlex (Turb)

The following optional extensions are available in specialist shops:
Device/cable Model
Barcode reader* ! Handscanner
Datalogic DLC6065-M1
! Handscanner
Datalogic Touch65
Connection cable Datalogic CAB-350
Barcode reader - LabStation
* In addition to the barcode reader, a suitable connection cable is

required to operate the barcode reader
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Accessories, options pHotoFlex / pHotoFlex Turb
98 ba75491d04 07/2006
pHotoFlex / pHotoFlex Turb Lists
9 Lists
This chapter provides additional information and orientation aids.
Abbreviations The list of abbreviations explains the indicators and the abbreviations
that appear on the display and in the manual.
Specialist terms The glossary briefly explains the meaning of the specialist terms. How-
ever, terms that should already be familiar to the target group are not
described here.
ba75491d04 07/2006 99
Lists pHotoFlex / pHotoFlex Turb
Abbreviations
°C Temperature unit, degrees Celsius

°F Temperature unit, degrees Fahrenheit
Asy Asymmetry
Cal Calibration
d Day
h Hour
j Year
K Temperature unit, Kelvin
LoBat Batteries almost empty (Low battery)
m Month
mV Voltage unit
mV/pH Unit of the electrode slope (internat. mV)
NIST/DIN Automatic pH calibration with buffer solutions pre-
pared according to NIST or DIN 19 266
pH pH value
s Second
S Slope (internat. k)
SELV Safety Extra Low Voltage
Slp. Slope determined with calibration

TEC Automatic pH calibration with WTW technical buff-
er solutions according to DIN 19267
U Voltage
100 ba75491d04 07/2006

Glossary
Adjusting To manipulate a measuring system so that the relevant value (e. g. the
displayed value) differs as little as possible from the correct value or a
value that is regarded as correct, or that the difference remains within
the tolerance.
Analysis specification The exact proceeding to carry out the detection procedure is described
in the analysis specification.
Asymmetry Designation for the offset potential of a pH electrode. It is the measur-
able potential of a symmetrical electrode, the membrane of which is
immersed in a solution with the pH of the nominal electrode zero point
(WTW electrodes: pH = 7).
AutoRange Name of the automatic selection of the measuring range.
AutoRead WTW name for a function to check the stability of the measured value.
Blank value (reagent The evaluation of the photometric measurement always refers to the
blank value) comparison value of a sample without the substance to be determined
(reagent blank value). Thus the influence of the basic absorbance of
the reagents on photometric measurement is compensated for.
Calibration Comparing the value from a measuring system (e. g. the displayed val-
ue) to the correct value or a value that is regarded as correct. Often,
this expression is also used when the measuring system is adjusted at
the same time (see adjusting).
Cell Vessel to take a liquid sample for measurement in a photometer.
The cell material (mostly glass) must have certain optical features to
be suitable for photometry.
Citation forms Different forms of representing a measured concentration value that
can be derived from each other.
The method to determine phosphate, e.g. delivers a measured value
for phosphorous P. This measured value can be alternatively quoted
in the citation forms, PO4, PO4-P or P2O5.
Detection procedure The detection procedure designates the general principle of how a
sample is brought into a form suitable for measurement.
Different methods can be based on the same detection procedure.
Electrode zero point The zero point of a pH electrode is the pH value at which the electro-
motive force of the pH electrode at a specified temperature is zero.
Normally, this is at 25 °C.
Electromotive force of The electromotive force U of the electrode is the measurable electro-
an electrode motive force of an electrode in a solution. It equals the sum of all the
galvanic voltages of the electrode. Its dependency on the pH results in
the electrode function which is characterized by the parameters, slope
and zero point.
ba75491d04 07/2006 101

Junction The junction is a porous body in the housing wall of reference elec-
trodes or electrolyte bridges. It forms the electrical contact between
two solutions and makes electrolyte exchange more difficult. The ex-
pression, junction, is also used for ground or junction-less transitions.
LED Light Emitting Diode
LEDs are used as the light source in the pHotoFlex (Turb).
Measured parameter The measured parameter is the physical dimension determined by
measuring, e. g. pH, conductivity or DO concentration.
Measured value The measured value is the special value of a measured parameter to
be determined. It is given as a combination of the numerical value and
unit (e. g. 3 m; 0.5 s; 5.2 A; 373.15 K).
Measuring system The measuring system comprises all the devices used for measuring,
e. g. meter and sensor. In addition, there is the cable and possibly an
amplifier, terminal strip and armature.
Method A method comprises a chemical detection procedure and special
method data (calibration line) that is required to evaluate the
measurement results.
How to carry out the method up to measuring with the photometer is
described in the analysis specification.
The pHotoFlex (Turb) contains a database with methods (programs).
Furthermore, user-defined methods can be entered in the database as
well.
Molality Molality is the quantity (in Mol) of a dissolved substance in 1000 g sol-
vent.
MultiCal® WTW name stating that a meter provides several pH calibration pro-
cedures.
Offset potential The measurable potential of a symmetrical electrode, the membrane
of which is immersed in a solution with the pH of the nominal electrode
zero point. The asymmetry is part of the offset potential.
ORP voltage The ORP is caused by oxidizing or reducing substances dissolved in
water if these substances become effective on an electrode surface (e.
g. a gold or platinum surface).
pH value The pH value is a measure of the acidic or basic effect of an aqueous
solution. It corresponds to the negative decadic logarithm of the molal
hydrogen ions activity divided by the unit of the molality. The practical
pH value is the value of a pH measurement.
Potentiometry Name of a measuring technique. The signal (depending on the mea-
sured parameter) of the electrode is the electrical potential. The elec-
trical current remains constant.
102 ba75491d04 07/2006

Program In the pHotoFlex (Turb), methods with the relevant method data are
stored as programs.
Programs are called up via the assigned program number.
Reset Restoring the original condition of all settings of a measuring system.
Resolution Smallest difference between two measured values that can be dis-
played by a meter.
Slope The slope of a linear calibration function.
Standard solution The standard solution is a solution where the measured value is
known by definition. It is used to calibrate a measuring system.
Temperature function Name of a mathematical function expressing the temperature behav-
ior of a test sample, a sensor or part of a sensor.
Test sample Designation of the test sample ready to be measured. Normally, a test
sample is made by processing the original sample. The test sample
and original sample are identical if the test sample was not processed.
Test set (test) A test set contains all reagents that are required for the photometric
determination of the sample according to the analysis specification.
Zero adjustment Adjusting a photometer with a water-filled cell.
The zero adjustment applies to measuring all measured parameters
(concentration, absorbance, transmission) of a photometer.
ba75491d04 07/2006 103

104 ba75491d04 07/2006

pHotoFlex / pHotoFlex Turb Index
10 Index
A system settings ............................ 76, 77
Accumulator pack Diluted samples
charging time .....................................17 measuring ......................................... 50
Air ..........................................................62 Display ............................................. 10, 38
Aligning and marking a cell .......................62 Display illumination ............................ 10, 24
Analysis specification ...............................40 Drift control
Analysis timer ..........................................49 ORP ................................................. 53
Asymmetry of the pH electrode .................55 pH .................................................... 52
Authorized use .........................................14
Automatic switchoff ............................18, 23 F
AutoRead Filter ...................................................... 70
ORP ..................................................53 Firmware update ................................... 107
pH .....................................................52
I
B Initial commissioning ............................... 21
Barcode reader ........................................19 Initialization ............................................. 76
Blank value .............................................43 Interface ................................................. 38
Blank value measurement ........................44
Buffer sets, pH .........................................55
K
Key functions ............................................ 8
C Keys ........................................................ 8
Calibrating
when? ...............................................65
M
Calibration
pH .....................................................55 Measured value display ........................... 27
turbidity measurement .........................65 Measurement dataset .............................. 68
Calibration order ......................................65 Measuring
Calibration points ORP voltage ...................................... 53
pH .....................................................56 pH .................................................... 52
Calibration points and measuring ranges ...65 Measuring menu
Calibration standards ...............................65 pH/ORP ...................................... 36, 54
Cell Measuring range exceeded ...................... 64
insert .................................................24 Measuring the absorbance/transmission ... 45
Cleaning .................................................85 Memory .................................................. 36
Concentration measurement .....................42 Menus (navigation) .................................. 28
Connecting a PC ......................................72 Messages ............................................... 28
Connecting a printer .................................72 Method ................................................... 40
Connecting a sensor ................................10
N
D Navigation .............................................. 27
Data filter ................................................70
Dataset ...................................................68 O
Date and time ..........................................31 Obligations of the operator ....................... 15
Date/time ................................................39 Operating modes .................................... 27
Delivery condition Operating safety ...................................... 14
measuring parameters ........................77
ba75491d04 07/2006 105

Index pHotoFlex / pHotoFlex Turb
P U
pH calibration evaluation .......................... 57 User-defined programs ............................ 48
Photometric determinations ...................... 40
Power pack ............................................. 17 Z
Print ....................................................... 75
Zero adjustment ...................................... 46
Program ................................................. 40
Programs ............................................... 48
R
Reset ..................................................... 76
RS232 socket assignment ....................... 73
S
Safe operation ........................................ 15
Safety .................................................... 13
Scope of delivery .................................... 17
Single-point calibration
pH .................................................... 59
Slope
pH .................................................... 55
Socket field ............................................. 10
Storage of measured values
storage locations ............................... 68
Storing ................................................... 68
Switching on ........................................... 23
System menu
General ............................................. 38
general information ............................ 41
System settings ...................................... 35
general ................................. 35, 38, 39
T
Target group ........................................... 13
Temperature measurement
pH .................................................... 51
Test set .................................................. 40
Three-point calibration
pH .................................................... 61
Timer ..................................................... 49
Transmitting data .................................... 72
Transmitting measured values .................. 72
Turbidity ................................................. 62
measuring ......................................... 63
Two-point calibration
pH .................................................... 60
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pHotoFlex / pHotoFlex Turb
Appendix: Firmware update

General information With the "Firmware Update pHotoFlex" program you can update the
firmware of the pHotoFlex (Turb) to the latest version with the aid of a
Personal Computer.
The update program contains:

! the newest firmware (meter software)
! new or changed method data and programs.
A free serial interface (COM port) on your PC and the AK 540/B inter-
face cable is required for this.
Program installation Install the firmware update program on your PC with the
"pHotoFlex_Vx_yy_English.exe" installation program.
Program start Start the "Update_pHotoFlex" program from the Windows start menu.
The program automatically selects the first free serial interface (COM
port). The selected interface is displayed on the left side of the status
line on the screen bottom.
Via the language menu you can change the adjusted language.
Firmware update Proceed as follows:
1 With the aid of the AK 540/B interface cable, connect the

pHotoFlex (Turb) to the serial interface (COM port) of the PC
named in the status line.
2 Make sure the pHotoFlex (Turb) is switched on.
3 To start the updating process click the OK button.
4 Then follow the instructions of the program.
A corresponding message and a progress bar (in %) appear
during the programming procedure.
The programming procedure takes approx. four minutes.
A final message appears after the successful programming
procedure. The firmware update is completed with this.
5 Disconnect the meter from the PC.
The instrument is ready for operation.
After switching the meter off and on again you can check on the start
display whether the meter has taken over the new software version.
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pHotoFlex / pHotoFlex Turb
108 ba75491d04 07/2006

Wissenschaftlich-Technische Werkstätten GmbH
Dr.-Karl-Slevogt-Straße 1
D-82362 Weilheim
Germany
Tel: +49 (0) 881 183-0

+49 (0) 881 183-100
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Operating Manual Photoflex WTW

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Operating Manual Photoflex WTW

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Operating manual

Firmware Part of the process of consequently improving our products is the

Copyright © 2006, WTW GmbH

5 Maintenance, cleaning, disposal . . . . . . . . . . . . . . . . . 83

7.1 General data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91

Appendix: Firmware update . . . . . . . . . . . . . . . . . . . . . . . 107

The pHotoFlex (Turb) handheld meter provides the maximum degree

The proven MultiCal ® calibration procedure supports you when cali-

You will find information on available literature in the WTW catalog or

Key functions Select the measuring mode

In the Photometry measuring mode: Select a pro-

Output display contents to RS232 interface (e.g.

Highlight menu items or selection

Example Photometry \ Concentration Measuring mode \ measured

1.4 Socket field

Identifying the 1 pH electrode

1.5 LabStation (optional)

Fig. 1-1 LabStation

The personnel responsible for the commissioning, operation and main-

2.1 Authorized use

The technical specifications as given in chapter 7 TECHNICAL DATA must

2.2 General safety instructions

Opening the photometer or adjustment, maintenance and repair work

The only exceptions to this are the activities described in chapter

Follow the points listed below when operating the photometer:

If the instrument was transported from a cold environment to a warm

If safe operation is no longer possible, the instrument must be taken out

3.2 Power supply

The LoBat display indicator appears when the batteries or accumulator

Charging time of the approx. 36 hours.

Use original power packs only (see chapter 7 TECHNICAL DATA).

Connecting the power 4

3.3 Connecting the LabStation

Connecting the LabSta-

4 Connect the power pack to an easily accessible power socket.

6 Connect a pH electrode to the socket on the pHotoFlex (Turb)

3.4 Initial commissioning

After a few seconds, the meter automatically switches to the measuring

With <M> (long pressure) change the measuring mode.

Switching off Press the <ON/OFF> key.

The automatic switchoff is not active

! if the power is supplied by the power pack (optional),

4.2 Inserting a cell

1 Push the dust cover (1) upward.

3 For turbidity measurement:

2 Pull up the height adapter (3).

3 Open the external light cover (4) of the cell shaft.

4 Insert the 16 mm cell (marking points forward) so that it is po-

4.3 General operating principles

4.3.1 Operating modes

The instrument has the following operating modes:

Measured value display In the measured value display, you can

4.3.3 Navigation example 1: Setting the language

1 In the measured value display:

5 Open the setting of the Language with <START/ENTER>.

6 Select the required language with <▲> <▼>.

8 To make further settings, switch to the next higher menu level

4.3.4 Navigation example 2: Setting the date and time

Numerals are generally entered via the number keys.

Therefore, check the time at regular intervals.

1 In the measured value display:

4 Enter the time using the number keys.