NAMA : SEPTI GIANA

NIM : 06101181722039
TUGAS BIOKIMIA II

1. Hasil sekuensing DNA, diperoleh pada gambar 1 disamping,

berdasarkan gambar 1 tersebut susunlah urutan DNA hasil
sekuensing tersebut.

Pembacaan kodon dimulai dari atas kebawah

- DNA
TCG AAA CAG AGA CCG TTG TAG TTA CTG
ACC TAA TAG CAA GTG TCT TAA CTA AAG
GGA GAG CTA GTA TAC TCA ACG TCT AGA
CTA TTG GAG CTG AAC TCC CGG CTC GGT
2. Dari suatu urutan DNA sebagai berikut

-DNA
5’
ATG GTG CAT TAC ACT CCT GAG GAGA*AG T*CT GCC GTT ACT GCC CTG TGG GGC AAG
GTG AAC GTG GAT GAA GTT GGT GGTGAG GCC CTG GGC AGG CTG CTG GTG GTC TAC CCT
TGG ACC CAG AGG TTC TTT GAG TCC TTT GGG GAT CTG TCC ACT CCT GAT GCT GTT ATG
GGC AAC CCT AAG GTG AAG GCT CAT GGC AAG AAA GTG CTC GGT GCC TTT AGT GAT
GGC CTG GCT CAC CTG GAC AAC CTC AAG GGC ACC TTT GCC ACA CTG AGT GAG CTG
CAC TGT GAC AAG CTG CAC GTG GAT CCT GAG AAC TTC AGG CTC CTG GGC AAC GTG
CTG GTC TGT GTG CTG GCC CAT CAC TTT GGC AAA GAA TTC ACC CCA CCA GTG CAG GCT
GCC TAT CAG AAA GTG GTG GCT GGT GTG GCT AAT GCC CTG GCC CAC AAG TAT CAC
TAA3’
Pertanyaan:
a. Jelaskan dan tuliskan sistem translasinya
-mRNA dan Asam Amino
UAC CAC GUA AUG UGA GGA CUC CUC UUC AGA CGG CAA UGA CGG
Tyr His Val Met end Gly Leu Leu Phe Arg Arg Gln End Arg
GAC ACC CCG UCC CAC UUG CAC CUA CUU CAA CCA CCA CUC CGG
Asp Thr Pro Phe His Leu His Leu Leu Gln Pro Pro Leu Arg
GAC CCG UCC GAC GAC CAC CAG AUG GGA ACC UGG GUC UCC AAG
Asp Pro Ser Asp Asp His Gln Met Gly Thr Trp Val Ser Lys
AAA CUC AGG AAA CCC CUA GAC AGG UGA GGA CUA CGA CAA UAC
Lys Leu Arg Lys Pro Leu Asp Arg End Gly Leu Arg Gln Tyr
CCG UUG GGA UUC CAC UUC CGA GUA CCG UUC UUU CAC GAG CCA
Pro Leu Gly Phe His Phe Arg Val Pro Phe Phe His Glu Pro
CGG AAA UCA CUA CCG GAC CGA GUG GAC CUG UUG GAG UCC CCG
Arg Lys Ser Leu Pro Asp Arg Val Asp Leu Leu Glu Phe Pro
UGG AAA CGG UGU GAC UCA CUC GAC GUG ACA CUG UUC GAC GUG
Trp Lys Arg Cys Asp Ser Leu Asp Val Thr Leu Phe Asp Val
CAC CUA GGA CUC UUG AAG UCC GAG GAC CCG UUG CAC GAC CAG
His Leu Gly Leu Leu Lys Ser Glu Asp Pro Leu His Asp Gln
ACA CAC GAC CGG GUA GUG AAA CCG UUU CUU AAG UGG GGU GGU
Thr His Asp Arg Val Val Lys Pro Phe Leu Lys Trp Gly Gly
CAC GUC CGA CGG AUA GUC UUU CAC CAC CGA CCA CAC CGA UUA
His Val Arg Arg Ile Val Phe His His Arg Pro His Arg Leu
CGG GAC CGG GUG UUC AUA GUG AUU 3’
Arg Asp Arg Val Phe Ile Val Ile
 b. Jika A* pada keadaan normalnya diganti (substitusi) dengan T, jelaskan dan tuliskan apa yang akan
terjadi

-DNA
5’
ATG GTG CAT TAC ACT CCT GAG GAG TAG TCT GCC GTT ACT GCC CTG TGG GGC AAG
GTG AAC GTG GAT GAA GTT GGT GGTGAG GCC CTG GGC AGG CTG CTG GTG GTC TAC
CCT TGG ACC CAG AGG TTC TTT GAG TCC TTT GGG GAT CTG TCC ACT CCT GAT GCT GTT
ATG GGC AAC CCT AAG GTG AAG GCT CAT GGC AAG AAA GTG CTC GGT GCC TTT AGT
GAT GGC CTG GCT CAC CTG GAC AAC CTC AAG GGC ACC TTT GCC ACA CTG AGT GAG
CTG CAC TGT GAC AAG CTG CAC GTG GAT CCT GAG AAC TTC AGG CTC CTG GGC AAC
GTG CTG GTC TGT GTG CTG GCC CAT CAC TTT GGC AAA GAA TTC ACC CCA CCA GTG
CAG GCT GCC TAT CAG AAA GTG GTG GCT GGT GTG GCT AAT GCC CTG GCC CAC AAG
TAT CAC TAA3’

-mRNA dan Asam Amino

UAC CAC GUA AUG UGA GGA CUC CUC AUC AGA CGG CAA UGA CGG
Tyr His Val Met end Gly Leu Leu Ile Arg Arg Gln End Arg
GAC ACC CCG UCC CAC UUG CAC CUA CUU CAA CCA CCA CUC CGG
Asp Thr Pro Phe His Leu His Leu Leu Gln Pro Pro Leu Arg
GAC CCG UCC GAC GAC CAC CAG AUG GGA ACC UGG GUC UCC AAG
Asp Pro Ser Asp Asp His Gln Met Gly Thr Trp Val Ser Lys
AAA CUC AGG AAA CCC CUA GAC AGG UGA GGA CUA CGA CAA UAC
Lys Leu Arg Lys Pro Leu Asp Arg End Gly Leu Arg Gln Tyr
CCG UUG GGA UUC CAC UUC CGA GUA CCG UUC UUU CAC GAG CCA
Pro Leu Gly Phe His Phe Arg Val Pro Phe Phe His Glu Pro
CGG AAA UCA CUA CCG GAC CGA GUG GAC CUG UUG GAG UCC CCG
Arg Lys Ser Leu Pro Asp Arg Val Asp Leu Leu Glu Phe Pro
UGG AAA CGG UGU GAC UCA CUC GAC GUG ACA CUG UUC GAC GUG
Trp Lys Arg Cys Asp Ser Leu Asp Val Thr Leu Phe Asp Val
CAC CUA GGA CUC UUG AAG UCC GAG GAC CCG UUG CAC GAC CAG
His Leu Gly Leu Leu Lys Ser Glu Asp Pro Leu His Asp Gln
ACA CAC GAC CGG GUA GUG AAA CCG UUU CUU AAG UGG GGU GGU
Thr His Asp Arg Val Val Lys Pro Phe Leu Lys Trp Gly Gly
CAC GUC CGA CGG AUA GUC UUU CAC CAC CGA CCA CAC CGA UUA
His Val Arg Arg Ile Val Phe His His Arg Pro His Arg Leu
CGG GAC CGG GUG UUC AUA GUG AUU 3’
Arg Asp Arg Val Phe Ile Val Ile

Pergantian satu basa A pada keadaan normal disubstitusikan dengan basa T hanya ada satu penerjemah
asam amino yang berubah pada triplet DNA TAG menjadi mRNA AUC asam amino nya menjadi Ile
 c. Jika T* pada keadaan normalnya dihilangkan (delesi), jelaskan dan tuliskan apa yang terjadi.
Jawab :
Hal tersebut menyebabkan bergesernya urutan pembacaan kodon DNA selama berlangsungnya sintesis
protein. Penerjemah asam amino juga berbeda.

DNA (Antisense)
5’
ATG GTG CAT TAC ACT CCT GAG GAG AAG CTG CCG TTA CTG CCC TGT GGG GCA AGG
TGA ACG TGG ATG AAG TTG GTG GTG AGG CCC TGG GCA GGC TGC TGG TGG TCT ACC
CTT GGA CCC AGA GGT TCT TTG AGT CCT TTG GGG ATC TGT CCA CTC CTG ATG CTG
TTA TGG GCA ACC CTA AGG TGA AGG CTC ATG GCA AGA AAG TGC TCG GTG CCT TTA
GTG ATG GCC TGG CTC ACC TGG ACA ACC TCA AGG GCA CCT TTG CCA CAC TGA GTG
AGC TGC ACT GTG ACA AGC TGC ACG TGG ATC CTG AGA ACT TCA GGC TCC TGG GCA
ACG TGC TGG TCT GTG TGC TGG CCC ATC ACT TTG GCA AAG AAT TCA CCC CAC CAG
TGC AGG CTG CCT ATC AGA AAG TGG TGG CTG GTG TGG CTA ATG CCC TGG CCC ACA
AGT ATC ACT AA 3’

Transkripsi menjadi mRNA

5’
UAC CAC GUA AUG UGA GGA CUC CUC UUC GAC GGC AAU GAC GGG
ACA CCC CGU UCC ACU UGC ACC UAC UUC AAC CAC CAC UCC GGG
ACC CGU CCG ACG ACC ACC AGA UGG GAA CCU GGG UCU CCA AGA
AAC UCA GGA AAC CCC UAG ACA GGU GAG GAC UAC GAC AAU ACC
CGU UGG GAU UCC ACU UCC GAG UAC CGU UCU UUC ACG AGC CAC
GGA AAU CAC UAC CGG ACC GAG UGG ACC UGU UGG AGU UCC CGU
GGA AAC GGU GUG ACU CAC UCG ACG UGA CAC UGU UCG ACG UGC
ACC UAG GAC UCU UGA AGU CCG AGG ACC CGU UGC ACG ACC AGA
CAC ACG ACC GGG UAG UGA AAC CGU UUC UUA AGU GGG GUG GUC
ACG UCC GAC GGA UAG UCU UUC ACC ACC GAC CAC ACC GAU UAC
GGG ACC GGG UGU UCA UAG UGA UU3’

Asam Amino dari mRNA

5’
Tyr – His–Val – Met – End– Gly –Leu – Leu – Phe –Asp – Gly – Asn-Asp – Gly-
Thr- Pro – Arg – Ser- Thr – Cys- Thr – Tyr – Phe- Asn – His – His- Ser- Gly-
Thr- Arg – Pro – Thr- Thr- Thr –Arg- Trp – Glu – Pro – Gly – Ser- Pro – Arg-
Asn – Ser – Gly – Asn – Pro – End –Thr- Gly – Glu- Asp – Tyr – Asp – Asn- Thr-
Arg – Trp – Asp – Ser- Thr – Ser- Glu – Tyr – Arg – Ser- Phe – Thr – Ser – His -
Gly – Asn – His – Tyr – Arg – Thr – Glu – Trp – Thr – Cys – Trp- Ser- Ser- Arg-
Gly – Asn- Gly – Val – Thr – His – Ser – Thr – End – His – Cys – Ser- Thr- Cys-
Thr- End – Asp– Ser – End– Ser– Pro – Arg – Thr – Arg – Cys – Thr – Thr – Arg -
His- Thr – Thr – Gly – End – End – Asn – Arg – Phe – Leu – Ser- Gly- Val- Val-
Thr- Ser - Asp - Gly - Gly - End – Ser – Phe – Thr - Asp – Asp – His – Asp – Tyr-
Gly – Thr – Gly – Cys – Ser – End – End-

Triplet DNA tersebut kehilangan basanya sehingga basa terus bergeser untuk pembacaan asam
aminonya dan mengakibatkan hampir seluruh asam amino yang terbaca berubah, serta pada akhirnya
tersisa dua basa yang tidak dapat diterjemahkan menjadi asam amino.