Ist professional MBBS

Table of specifications
Microbiology
S.NO MAIN Teachin Learning objectives DOMAINS (%) ASSESSMENT
. CONTENT g TOOLS (no.)
AREAS learning C P A MCQS SEQS
strategy
BLOCK 1
1. Introduction LGIS x 1  Comparison & C
to important features of
microbiology Medically Important
Organisms
 Enlist the major groups
of micro-organisms.
 Compare medically
important organisms
 Describe the method
of naming bacteria,
fungi, protozoa, and
helminths using the
binomial Linnean
system.
2. Bacteria LGIS x 1  Differentiate between C
compared prokaryotes and
with other eukaryotes
microorganis  Outline biological
m relationship of
pathogenic
microorganisms

3. Classification LGIS x 2  Classify medically C

of medically important bacteria on
important the basis of their
bacteria characteristics.
 Classify the special
groups of
microorganisms i.e.
Rickettsia, Chlamydia,
Mycoplasma &
Mycobacteria

4. Structure of LGIS x 2  Differentiate between C

bacterial cells the gram positive and
gram negative
bacteria.
 Discuss structures
outside the bacterial
cell membrane
 Discuss structures
inside the bacterial cell
membrane
 Ist professional MBBS
Table of specifications
Microbiology
5. Bacterial LGIS x 1  Illustrate Growth cycle C
growth of bacteria,
 Explain aerobic and
anaerobic bacterial
growth,
 Describe how bacterial
identification is done
using fermentation of
sugars and iron
metabolism

6. Important SGD x 1  Enlist various bacterial C A

bacterial virulence factors.
toxins  Describe their
mechanisms of actions
and their regulation
(exotoxin, endotoxin,
and their contribution
to pathogenesis).
7. Safety PRACTIC  Demonstrate safe P
precautions AL x1 practices in a
microbiology
laboratory.
8. Sample PRACTIC  Demonstrate various P
collection AL x 1 sample collection
techniques used in the
microbiology laboratory.
BLOCK 2

9. Bacterial LGIS x 1 Discuss various types of C

genetics bacterial cell mutations
. Discuss transfer of DNA
between bacterial cells
Discuss transfer of DNA
between bacterial cells
a. Conjugation
b. Transduction
c. Transformation
d. Describe types of
bacterial
recombination
10. Normal flora LGIS x 2 • Define normal flora, C
commensals, carriers,
colonization and colonization
resistance.
Enlist normal flora of various
 Ist professional MBBS
Table of specifications
Microbiology
anatomical locations
11. Pathogenesi LGIS x 2 • Define virulence of C
s of bacteria bacteria.
• Explain general
concepts of Pathogenesis &
host defense against
microorganisms
Explain Infection process
(development, and outcomes).
12. Host SGD x 1 Enumerate principles of host C A
defences defences.
Discuss innate immunity.
Discuss acquired immunity.
1. Gram PRACTICA Perform gram staining. P
staining Lx1 Examine the stained slide
under the microscope.
2. ZN staining PRACTICA Perform ZN staining. P
Lx1 Examine the stained slide
under the microscope.

BLOCK 3
13. Antimicrobia LGIS x 2 • Define the following: C
l drugs: antibacterial spectrum,
mechanism bacteriostatic, bactericidal,
of action antibiotic synergism, selective
toxicity, antibiotic antagonism,
Minimum inhibitory
concentration (MIC) and
minimum bactericidal
concentration (MBC).
• Classify anti-microbial
drugs with examples (anti-
bacteria’s, anti-viral, anti-
fungal, anti-helminthes).
Explain their modes of action.
14. Antimicrobia LGIS x1 • Define bacterial C
l drugs resistance and describe the
resistance mechanisms involved in
acquiring bacterial resistance.
• Describe mechanisms
involved in transfer of drug
resistance to bacterial
resistance.
Define Superinfection and
cross sensitivity.
15. Sterilization LGIS x 2 • Differentiate C
& disinfection and sterilization.
disinfection • Describe clinical uses
of disinfection and
 Ist professional MBBS
Table of specifications
Microbiology
sterilization.
• Enlist various types of
physical agents for disinfection
and sterilization.
• Describe Mechanism
of action of Sterilization by dry
heat and moist heat, radiation
and filtration.
• Describe mechanism
of action of sterilization by
chemical agents via: Disruption
of cell membranes,
Modification of proteins and
Modification of nucleic acids.
16. Laboratory SGD x 1 • Enlist various C A
diagnosis of diagnostic techniques used in
various microbiology lab.
microorgani •
sms • Enlist the biochemical
tests used for bacterial
isolation
• Describe general
principles of the following
tests:
I. ELISA – Hepatitis
(A,B,C,D,E,G) Rubella, CMV and
HIV
II. PCR
III. Haemagglutination –
TPHA
IV. Western Blot –HIV
Malaria.
1. Sterilization PRACTICA Identify various parts of P
& Lx1 autoclave and hot air ovene
disinfection working of hot air oven .
Demonstrate the sterilization
technique used for
sterilization/disinfection of
glass ware and needles in
using hot air oven and
autoclave.
2. Bacteria PRACTICA Identify various types of P
culture Lx1 culture media and perform
technique inoculation technique.

Total teaching hours microbiology: 35

LGIS: 17
Ist professional MBBS
Table of specifications
Microbiology
SGD: 6

Practical: 12