Krebs cycle is also known as the
tricarboxylic acid cycle/ Citric Acid Cycle and
it is a biochemical pathway that is used to
generate energy to the oxidation of
acetyl-coa it is also used for the
synthesis of NADH and for the production
of amino acids krebs cycle takes place
in the mitochondria of the eukaryotes
and the cytosol of the prokaryotes.
The first step involved is actually a
preparatory step which begins with
pyruvate. Pyruvate is derived through the
glycolysis of glucose which is a six
carbon compound and it is split into two
molecules of pyruvate which is a three
carbon compound.
The next step includes
the oxidation of pyruvate into
acetyl-coa by the enzyme pyruvate
dehydrogenase complex and in this
reaction a molecule of carbon dioxide
and a molecule of NADH is generated
The acetyl -coa is a two carbon compound in
the next step the acetyl co a combines
with Oxaloacetate which is a four
carbon compound to form citrate and
hence the resulting molecule is a six
carbon compound this reaction is
catalyzed by the enzyme citrate synthase
In the next step the citrate is
isomerized in to isocitrate by the
enzyme aconitase.
Next the isocitrate is oxidized into
alpha ketoglutarate by the enzyme
isocitrate dehydrogenase and in this
reaction a molecule of NAD is reduced to
NADH and a molecule of carbon dioxide is
generated.
Hence the alpha-ketoglutarate is the
five carbon compound since one molecule
of carbon is lost into carbon dioxide.
Next the alpha ketoglutarate is
converted into succinyl coa by the enzyme
alpha ketoglutarate dehydrogenase and in
this reaction also
a molecule of nad is reduced to NADH and
a molecule of carbon dioxide is released.
Hence the succinyl coa a is the four carbon
Compound.
In the next step this succinyl coa is converted
into succinate by
the enzyme Succinyl CoA Synthase in this
reaction a molecule of GTP is generated
Next, succinate is converted into Fumrate by
the enzyme succinate dehydrogenase.
In this reaction a molecule of QS2 is
generated which is used for the
production of FADH2.
Fumarate is then
converted into malate by the enzyme fumrase six NADH
two FADH2
two GTP and
four carbon dioxide

All the NADH and FADH2 are next fed to the

electron transport chain for the
generation of ATP.

In the last step the malate is

converted into oxaloacetate by the
enzyme malate dehydrogenase and in this
reaction also NAD is reduced to NADH.

RESULT OF KREBS CYCLE

The results of the Krebs

cycle through each cycle of the krebs
cycle following products are generated:
three molecules of NADH
one molecule of FADH2
one molecule of GTP and
Two molecules of carbon dioxide

Since glucose is split into two

pyruvate compounds for each molecule of
glucose this cycle runs twice
hence the products formed are: