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Phase fraction calculation of microstructure using ImageJ

Method · December 2018

DOI: 10.13140/RG.2.2.22990.51521

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V S Hariharan
Indian Institute of Technology Madras
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 Phase fraction
calculation of
microstructure using
ImageJ
BY
HARIHARAN V S

HARIHARAN V S OPENMET IMAGEJ 1

 STEP-1

1. Open ImageJ sotware

2. Select File  Open

HARIHARAN V S OPENMET IMAGEJ 2

 STEP-2

1. Select the required file

2. Click Open

HARIHARAN V S OPENMET IMAGEJ 3

 STEP-3

The selected image appears

HARIHARAN V S OPENMET IMAGEJ 4

 STEP-4

1.Select ImageDuplicate
2. Another copy of the image is created
and file name prompt appears

HARIHARAN V S OPENMET IMAGEJ 5

 STEP-5

1.Enter the name of duplicate file (which is of your wish)

2. Click OK
3. The duplicate image appears

HARIHARAN V S OPENMET IMAGEJ 6

 STEP-6

Click on the rectangular box icon

HARIHARAN V S OPENMET IMAGEJ 7

 STEP-7

The area other than the

bottom unwanted area is
selected

This area should not be counted

for analysis

HARIHARAN V S OPENMET IMAGEJ 8

 STEP-8

Select Image Crop

HARIHARAN V S OPENMET IMAGEJ 9

 STEP-9

Select ImageType8-bit

HARIHARAN V S OPENMET IMAGEJ 10

 STEP-10

Select ImageAdjustThreshold

HARIHARAN V S OPENMET IMAGEJ 11

 STEP-10

Adjust the
scale to colour
the phase of
interest

HARIHARAN V S OPENMET IMAGEJ 12

 STEP-11

Adjust the scale such that only the

phase of interest is coloured red and
then click APPLY

HARIHARAN V S OPENMET IMAGEJ 13

 STEP-12

Select Analyze Analyze Particles

HARIHARAN V S OPENMET IMAGEJ 14

 STEP-13

1.Ensure that “summarize” option is checked

2.Click OK

The phase fraction of

shaded region is
obtained

HARIHARAN V S OPENMET IMAGEJ 15

 CAUTION
The above procedure should be carried out for a large number of images taken in the
same magnification and the average value should be reported.
The obtained phase fraction will vary for different threshold values. Hence, it is
necessary to choose the optimum threshold value which shades only the region/phase of
interest.
The images chosen for analyses should be clear and free of scratches.
If the phases don’t have a sharp contrast difference, it will be difficult to get accurate
results.

HARIHARAN V S OPENMET IMAGEJ 16

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