Академический Документы
Профессиональный Документы
Культура Документы
08.1.1 Introduction
This module unit is intended to equip the trainee with relevant knowledge about the
existence, classification and role of micro-organisms in food and public health. In
addition, the trainee will be equipped with knowledge and practical skills in handling,
analyzing and enumerating food microorganisms in the laboratory situations and food
processing. The module unit aims at imparting the trainee with skills for carrying out
sampling and practical skills in isolation, enumeration, identification and control of
microorganisms associated with foods. It brings awareness to the trainee about the
existence of food poisoning micro organisms and respective food borne diseases. The
trainee is equipped with knowledge and skills in preventing food borne diseases in every
day life and laboratory situations
08.1.17 Parasites
Protozoa.
Helminths -
Tapeworms, 8 2 10
pinworms, and
roundworms
08.1.18 Emerging Issues and •Emerging issues and
Trends trends
•Challenges posed by
emerging issues and
trends
•Ways of managing
challenges posed by
emerging issues and
trends
Total
08.1.01T INTRODUCTION TO Content
FOOD MICROBIOLOGY AND 08.1.01P2 Application of
PARASITOLOGY microbiology
Theory Suggested
Teaching/Learning
08.1.01T Specific Objectives Activities
By the end of the sub- - Discussion
module unit, the trainee - Demonstration
should be able to: - Lecture
a) define terms applied in - Practical work
microbiology
b) outline the background Suggested
of microbiology Teaching/Learning
c) explain the general Resources
importance of microbiology
- Sample of Live
08.1.01 C Competence microorganism
The trainee should have - fermented food
the ability to apply the samples
acquired skills of - Text books
microbiology in food - diagrams
industry and public
health Suggested Evaluation
Methods
Content - Written tests
08.1.01T1 Definition of terms - Assignments
- Microbiology - Oral tests
- Microorganisms
08.1.01T2 Background of
microbiology 08.1.02 ROLE AND
08.1.01T08.1.14 Importance SIGNIFICANCE OF
of MICROORGANISMS
microbiology
Theory
Practice
08.1.02T0 Specific Objectives
08.1.01P0 Specific Objectives By the end of the sub-
By the end of the sub- module unit, the trainee
module unit, the trainee should be able to:
should be able to apply a) explain the significance
microbiology in of microorganisms
processing foods
b) discuss the role of - Human activities
microorganisms to the Food production
environment and human activities through fermentation
Food borne diseases
08.1.02 C Competence Food spoilage
The trainee should have Energy production
the ability to: As food
i) Identify the
Production of
significance of
antibiotics
microorganisms to the
environment and human
Practice
activities
08.1.02P0 Specific Objectives
ii) Apply microorganisms
By the end of the sub-
in the food industry
module unit, the trainee
should be able to apply
Content
microorganisms to
08.1.02T1 Significance of
environment and food
microorganisms
processing
- Food production and
preservation through fermentation
Content
- Source of food
08.1.02P1 application of
- Source of pure enzymes
microorganisms to the
- Production of
environment and human
antibiotics
activities
- Production of single
cell protein
Suggested
- Decomposition of
Teaching/Learning
matter
Resources
- Food spoilage
- Text books
- Food poisoning and
- Diagrams
food borne diseases
- Pest control
Suggested
- Purification of air
Teaching/Learning
08.1.02T2 Role of microorganisms
Activities
to environment and
- Practical work
human activities
- Field trips
- Environment
- Demonstrations
Decomposition of death - Discussions
organic matter - presentations
Recycling of
elements/nutrients around the food Suggested Evaluation
chain Methods
Pest control - examinations at the end
Purification of air of the module
- test
- oral test Practice
- assignments 08.1.03P0 Specific Objectives
By the end of the sub-
08.1.03 CELL AND module unit, the trainee
STRUCTURE OF should be able to:
MICROORGANISM a) draw a cross-section of
procaryotic cell, yeast, mould and
Theory viruses
b) label the different
08.1.03T0 Specific Objectives structures of bacterial cells, fungi
By the end of the sub- and viruses
module unit, the trainee
should be able to: Content
a) describe prokaryotic 08.1.03P1 Cross-section of
cells and eukaryotic cells procaryotic cell, yeast,
b) explain the structures mould and viruses
of prokaryotic and eukaryotic cells 08.1.03P2 Structures of
c) describe the structure of procaryotic cell, yeast
fungi mould and viruses
d) discuss the structure of
viruses Suggested
08.1.03 C Competence Teaching/Learning
The trainee should have Resources
the ability to: - text books
i) Identify prokaryotic - diagrams
cells and eukaryotic cells - photographs
ii) Differentiate the - internet
bacteria, fungi and viruses
Suggested
Content Teaching/Learning
08.1.03T1 prokaryotic cells and Activities
eukaryotic cells - Discussions
- Prokaryotic cells - Demonstrations
- Eukaryotic cells - Lecture
- Table of comparison - Project work
08.1.03T2 Structures of
prokaryotic and Suggested Evaluation
eukaryotic cells Methods
08.1.03T08.1.14 Structures of - Examination at end of
fungi module
- Yeast - Tests
- Mould - Practical assignment
08.1.03T4 Structures of viruses
08.1.04 GROWTH OF Suggested
MICROORGANISMS Teaching/Learning
Resources
Theory - Text books
- Diagrams
08.1.04T0 Specific Objectives - Microorganisms manual
By the end of the sub- - Internet
module unit, the trainee
should be able to: Suggested
a) describe factors Teaching/Learning
affecting the growth of Activities
microorganisms - Discussions
- Intrinsic parameters - Demonstrations
- Extrinsic parameters - Lecture
b) describe reproduction in - Project work
microorganisms
c) explain microbial Suggested Evaluation
growth curves Methods
d) discuss cultivation - Examination at end of
methods of microorganisms module
- Tests
08.1.04 C Competence - Practical assignment
The trainee should have
the ability to: 08.1.05 MICROSCOPY
i) Culture
microorganisms Theory
ii) Differentiate the
bacteria, fungi and viruses 08.1.05T0 Specific Objectives
By the end of the sub-
Content module unit, the trainee
08.1.04T1 Factors affecting the should be able to:
growth of a) identify various parts of
microorganisms a modern light microscope
- Intrinsic parameters b) describe the use, care
- Extrinsic parameters and maintenance of microscope
08.1.04T2 Reproduction in c) describe different types
microorganisms of microscopes
08.1.04T08.1.14 Microbial
growth 08.1.05 C Competence
curves The trainee should have
08.1.04T4 Cultivation methods the ability to:
i) identify parts of a
microscope
ii) use, care and maintain a b) use , care and maintain
microscope a microscopes
Content Content
08.1.05T1 Parts of a modern light 08.1.05P1 Parts of a microscope
microscope 08.1.05P2 Care and maintain a
- Ocular microscopes
- Nose piece
- Objectives
- Stage focussing knob - Prepare smears
- Condenser - Focussing using
different magnifying objectives
- Diaphragm
- Arm Suggested
- Light source Teaching/Learning
08.1.05T2 Use, care and Activities
maintenance of - Discussion
microscope - Demonstration
- Focussing using - Lecture
different objectives - Practical work
- Preparing a smear
- Microscope cleaning Suggested
- Cleaning objective lens Teaching/Learning
- Cleaning high power Resources
objective
08.1.05T08.1.14 Types of - Sample of live
microscopes microorganism
- Light microscopes - fermented food
- Bright field samples
microscopes - Text books
- Dark field microscopes - diagrams
- Phase contrast
microscopes Suggested Evaluation
- Fluorescence Methods
microscopes - Written tests
- Electron microscopes - Assignments
- Oral tests
Practice
08.1.05P0 Specific Objectives 08.1.06 LABORATORY
By the end of the sub- EQUIPMENT AND
module unit, the trainee STERILIZATION
should be able to:
a) identify parts of a Theory
microscope
08.1.06T0 Specific Objectives By the end of the sub-
By the end of the sub- module unit, the trainee
module unit, the trainee should be able to:
should be able to: a) identify basic
a) identify basic apparatus microbiology laboratory apparatus
used in microbiological work b) carry out sterilization of
b) describe methods used different apparatus in the
for sterilization of different laboratory
laboratory equipments
Content
08.1.06 C Competence 08.1.06P1 Identifying basic
The trainee should have microbiology laboratory
the ability to: apparatus
i) Identify basic 08.1.06P2 Sterilization
apparatus used in - Dry heat
microbiological work Flaming wire loop
ii) Sterilize different Flaming glass spreader
laboratory equipments - Wet heat sterilization
- Autoclaving
Content - Tyderllization
08.1.06T1 Basic apparatus
- Petri dishes Suggested
- Microscope slides Teaching/Learning
- Inoculation loop Activities
- Pipette - Discussion
- Autoclave - Demonstration
- Incubators - Lecture
- Oven - Practical work
- Glass rods Suggested
- Spreader
- Flasks Teaching/Learning
08.1.06T2 Sterilization methods Resources
- Sterilization in absence - Sample of Live
of moisture microorganisms
- Sterilization in presence - fermented food
of moisture samples
- Disinfection - Text books
- Irradiation - diagrams
- Food preservation
Suggested Evaluation
Practice Methods
08.1.06P0 Specific Objectives - Written tests
- Assignments
- Oral tests
By the end of the sub-
08.1.07 STAINING module unit, the trainee
should be able to:
Theory a) prepare a microscopic
slide smear
Theory b) carry out different
staining procedures
08.1.07T0 Specific Objectives
By the end of the sub- Content
module unit, the trainee 08.1.07P1 Preparation of smear
should be able to: 08.1.07P2 Staining procedures
a) explain the importance - Positive and negative
of staining staining
b) identify types of dyes
c) explain how dyes work Suggested
d) describe different Teaching/Learning
staining procedures Activities
- Discussion
08.1.07 C Competence - Demonstration
The trainee should have - Lecture
the ability to: - Practical work
i) Identify staining dyes
ii) Use dyes to stain Suggested
specimen Teaching/Learning
Resources
Content - Text books
08.1.07T1 Importance of staining - Basic equipments
08.1.07T2 Types of dyes - diagrams
- Positive staining
dyes Suggested Evaluation
- Negative staining Methods
dyes - Written tests
08.1.07T08.1.14 working of - Assignments
dyes - Oral tests
08.1.07T4 Staining procedures
- Gram stain procedures 08.1.08 CULTURING
- Acid fast staining
- Endospore staining Theory
- Flagella staining
- Capsule staining 08.1.08T0 Specific Objectives
- Preparation of smear By the end of the sub-
module unit, the trainee
Practice should be able to:
08.1.07P0 Specific Objectives a) define the basic terms
b) identify the different 08.1.08T4 Culturing and
types of culture media incubation methods
c) explain composition of - Culturing methods
culture media Pour plate method
d) describe culturing and Spread plate method
incubation methods Streaking method
e) explain methods of Stabbing method
isolation of microorganisms to - Incubation methods
pure culture Aerobic
Anaerobic
08.1.08 C Competence
The trainee should have Microaerophillic
the ability to: CO2
i) Prepare culture Temperature
media for culturation Humidity
of different 08.1. 08T5 Methods of isolation of
microorganisms microorganisms to pure
ii) Isolate culture
microorganisms to - Streak plate
pure culture - Absolute dilution
technique
Content - Pour plate
08.1.08T1 Definition basic terms
- Culture media Practice
- Colony forming unit 08.1.08P0 Specific Objectives
(CFM) By the end of the sub-
- Isolation module unit, the trainee
08.1.08T2 Types of culture media should be able to:
- General purpose media a) identify the different
- Selective media components of culture media
- Differential media b) prepare culture media
- Enrichment media c) isolate microorganisms
08.1.08T08.1.14 to pure culture
Composition of
culture media Content
- Carbon source 08.1.08P1 Components of culture
- Nitrogen source media
- Dye - Carbon source
- Vitamin source in - Nitrogen source
specific media - Dye
- Agar - Vitamin source in
- Water specific media
- In organic ions - Agar
- Water
- In organic media 08.1.09 FOOD SAMPLING
08.1.08P2 Preparation of culture
media Theory
- General purpose media
- Selective media 08.1.09T Specific Objectives
- Enrichment media By the end of the sub-
08.1.08P08.1.14 Isolation of module unit, the trainee
microorganisms should be able to:
- Streak plating a) explain the necessity of
- Absolute dilution food sampling
technique b) carry out sampling of
- Pour plate technique liquid and solid food materials
c) describe surface
Suggested sampling techniques
Teaching/Learning
Activities 08.1.09 C Competence
- Discussion The trainee should have
- Demonstration the ability to:
- Lecture i) Sample liquid and
- Practical work solid food materials
ii) Apply surface
Suggested sampling techniques
Teaching/Learning
Resources Content
- Sample of Live 08.1.09T1 Necessity of food
microorganism sampling
- fermented food 08.1.09T2 Sampling
samples - Liquid sampling
- Text books - Solid food materials
- Diagrams sampling
- Media 08.1.09T08.1.14 Surface
sampling techniques
Suggested Evaluation - Surface slices
Methods - Rinses and washes
- Written tests - Swabs
- Assignments - Adhesive tapes
- Oral tests - Agar sausage
- Impression plates
- Contact slide
Practice
08.1.09P0 Specific Objectives
By the end of the sub-
module unit, the trainee a) implement
should be able to: microbiological specifications
a) Carry out sampling of b) follow microbiological
liquids and solid food materials guidelines
b) Use surface sampling
techniques Content
22.2.02P1 Microbiological
Content standards
08.1.09P1 Sampling of liquids and 22.2.02P2 Microbiological
solid food materials specifications
- Liquid sampling 22.2.02P08.1.14
- Solid sampling
08.1.09P2 Surface sampling Microbiological
techniques guidelines
- Surface slices
- Rinses and wastes Suggested
- Swabs Teaching/Learning
- Adhesive tapes Resources
- Agar sausage - Textbooks
- Impression plates - Journals
- Contact slide - Standard tables
- Charts
Suggested - Training manuals
Teaching/Learning
Resources Suggested
- Training manuals Teaching/Learning
- White board Activities
- LCD - Group discussions
- Projector - Illustrations
- Computer
Suggested Evaluation
Suggested Methods
Teaching/Learning - Tests
Activities - Questions and answers
- Discussions
- Tutorials 08.1.10 CONCEPT OF
- Demonstrations INDICATOR
ORGANISMS
Suggested Evaluation
Methods Theory
- Course work 08.1.10T0 Specific Objectives
- Continuous assessment
tests
By the end of the sub- b) examine for faecal
module unit, the trainee coliforms
should be able to:
a) explain the concept of Content
using organisms as indicators of 08.1.10P1 Assessing for total
quality and safety coliforms
b) carry out 08.1.10P2 Examining for faecal
microbiological assessment of coliforms
total coliforms - Streptococci