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Exercise 2: Skeletal Muscle Physiology: Activity 1: The Muscle Twitch and the Latent Period Lab Report

Pre-lab Quiz Results


You scored 100% by answering 5 out of 5 questions correctly.

1. Skeletal muscles are connected to bones by


You correctly answered: b. tendons.

2. Skeletal muscles are composed of hundreds to thousands of individual cells called


You correctly answered: c. fibers.

3. The term motor unit refers to


You correctly answered: c. one motor neuron and all of the skeletal muscle fibers it innervates.

4. The motor neuron and muscle fiber intersect at what is called


You correctly answered: d. the neuromuscular junction.

5. A twitch is
You correctly answered: a. one contractile response to a single action potential.

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Experiment Results
Predict Question:
Predict Question: Will changes to the stimulus voltage alter the duration of the latent period?
Your answer : a. Yes, changing the stimulus voltage will change the latent period duration proportionately.

Stop & Think Questions:


What is the period of time that elapses between the generation of an action potential and the start of muscle tension
development in a muscle fiber?
You did not answer this question.
Correct answer: c. the latent period

What occurs during the latent period of these isometric contractions?


You did not answer this question.
Correct answer: b. All the steps of excitation-contraction coupling occur.

Experiment Data:

Voltage Length Active Force Passive Force Total Force Latent Period
0.0 75 0.0 0.0 0.0 ----
3.0 75 1.04 0.0 1.04 ----
4.0 75 1.32 0.0 1.32 3.20
6.0 75 1.65 0.0 1.65 2.80
8.0 75 1.81 0.0 1.81 2.80
10.0 75 1.82 0.0 1.82 2.80

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Post-lab Quiz Results
You scored 100% by answering 6 out of 6 questions correctly.

1. An action potential in a motor neuron triggers the release of which neurotransmitter?


You correctly answered: b. acetylcholine

2. The term skeletal muscle fiber refers to


You correctly answered: a. an individual skeletal muscle cell.

3. The graded depolarization in the skeletal muscle fiber that is elicited in response to one action potential from the motor
neuron is called
You correctly answered: c. an EPP (end-plate potential).

4. Which of the following is not a phase of a skeletal muscle twitch?


You correctly answered: b. hyperpolarization phase

5. A skeletal muscle twitch is


You correctly answered: a. one contractile response to a single action potential.

6. Which of the following correctly matches the twitch phase with its definition?
You correctly answered: d. the contraction phase: the time between the end of the latent period and peak muscle tension

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Review Sheet Results
1. Define the terms skeletal muscle fiber, motor unit, skeletal muscle twitch, electrical stimulus, and latent period.
Your answer:
skeletal muscle- skeletal muscle cells
motor unit- consists of one motor neuron and all the muscle fiber
eletrical stimulus- the stimulus that activates the end plate potential
latent period- time between the action potential and the muscle contraction

2. What is the role of acetylcholine in a skeletal muscle contraction?


Your answer:
Ach is released during the end plate potential, Ach diffuses into the muscle's fiber plasma membrane and bind to
receptorsin the motor end plate initiating a change in the ion permeability that results in a graded depolarization of the
muscle plasmamembrane

3. Describe the process of excitation-contraction coupling in skeletal muscle fibers.


Your answer:
the entire process of the end plate potential

4. Describe the three phases of a skeletal muscle twitch.


Your answer:
time between action potential and muscle contraction

5. Does the duration of the latent period change with different stimulus voltages? How well did the results compare with
your prediction?
Your answer:
the active force of the muscle increases the stimulus voltage increases.

6. At the threshold stimulus, do sodium ions start to move into or out of the cell to bring about the membrane
depolarization?
Your answer:
the sodium channels start moving into the cell.

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