tiempo (h) Azucares PMA R [-Rs] C_s C_PMA 1/R

0 113.39 3.22
18.62 57.72 12.92 0.354655269 0.996078153 85.555 8.07 2.819639486
30.83 57 16.64 0.340494599 0.033780742 57.36 14.78 2.936904146
43.4 56.22 19.72 0.325916416 -0.12906025 56.61 18.18 3.068271345
54.54 55.2 21.67 0.31299669 0.038133359 55.71 20.695 3.19492197
68.19 54.66 25.23 0.297165966 0.318397511 54.93 23.45 3.365122914
81.12 46.65 28.96 0.282170269 0.492207718 50.655 27.095 3.543959483
91.53 45.39 32.36 0.270097167 0.532949678 46.02 30.66 3.7023713
102.66 34.8 35.27 0.257189038 0.499003845 40.095 33.815 3.888190594
115.57 34.19 42.38 0.242216537 0.420865635 34.495 38.825 4.128537272
127.42 27.79 45.62 0.228473381 0.378070804 30.99 44 4.376877501
139.29 20.74 45.96 0.21470703 0.403431584 24.265 45.79 4.65750936
151.15 16.27 47.11 0.200952276 0.483810662 18.505 46.535 4.976305917
163.36 11.16 51.4 0.186791606 0.524897151 13.715 49.255 5.353559613
174.51 6.68 51.82 0.173860282 0.340889255 8.92 51.61 5.751744943
188.17 6.08 52.81

PMA Derivada
Sustrato f[Ra] Polynomial (Sustrato)
-5.80E-04 2 PMA -1.16E-03 Polynomial (PMA) Lineweaver-Burk
3.76E-01
120 1 3.76E-01 3.5
4.1337 f(x) = 1.67159E-10 x⁶ − 1.0599E-07 x⁵ + 2.63211E-05 x⁴ − 0.00320909 x³ 3
100 + 0.194884 x² − 5.53381 x + 113.039 2.5
R² = 0.996357108185497
Azucar Derivada f[-R] 2
1/[-r]

80
1.67E-10 6 1.00296E-09 1.5
60
-1.06E-07 5 -5.2995E-07 1
g/L

f(x) = − 0.000579877148303 x²1.05E-04

+ 0.376252230819976 x + 4.13373220426927 0.5
2.63E-05 4
R²40= 0.989154940861414 0
-3.21E-03 3 -9.63E-03 0 0.02 0.04 0.06 0.08 0.1 0.12
20
1.95E-01 2 3.90E-01 1/C
-5.53E+00 1 -5.53E+00
0
1.13E+02 0 20 40 60 80 100 120 140 160 180 200
tiempo (h) m 3.014477295 2.217348472
5.171520796 0.264709575
r 0.040741152 0.463709772
0.339771919 8
0.073060052 1.720214022

Vmax 0.450989104
Km 1.359496414
 1/[-Rs] 1/C_s 1/C_PMA [-Rs]/C_s C_s/[-Rs]

1.003937288 0.011688388 0.123915737 85.21946639 0.011734408

29.60266492 0.017433752 0.067658999 1.937663388 0.516085511
-7.74831889 0.017664724 0.055005501 -7.30610095 -0.13687191
26.22375836 0.017950099 0.04832085 2.124409447 0.470719052
3.14072807 0.018204988 0.042643923 17.48957528 0.057176917
2.031662575 0.019741388 0.036907178 24.93278196 0.040107839
1.876349759 0.021729683 0.032615786 24.52634418 0.040772485
2.003992575 0.024940766 0.029572675 20.00755916 0.049981109
2.376055245 0.028989709 0.0257566 14.51776009 0.068881149
2.645007206 0.032268474 0.022727273 11.7164142 0.085350345
2.478735034 0.041211622 0.021838829 9.789267377 0.10215269
2.06692427 0.054039449 0.021489202 8.952916308 0.111695448
1.905135126 0.072912869 0.020302507 7.198964427 0.138908868
2.933504018 0.112107623 0.01937609 3.040732157 0.328868163

r-Burk Eadie-Hofstee Hanes-Woolf

0.6 0.16
0.5 0.14
0.12
0.4
0.1
0.3 0.08
C/-r
-r

0.2 0.06
0.04
0.1
0.02
0 0
.06 0.08 0.1 0.12 0 5 10 15 20 25 30 10 15 20 25 30 35 40 45 50 55 6
/C -r/C C

b m 0.01928838 0.065055996 b m -0.00224678 0.155530371

0.003975437 0.071777217 0.000334681 0.01251181
r 0.824812376 0.077727768 r 0.865557897 0.013579119
23.54082887 5 45.06702244 7
0.14222441 0.030208029 0.00831002 0.001290747

Vmax 0.065055996 Vmax 445.0820334

Km 0.01928838 Km 69.22377384
Woolf

35 40 45 50 55 60
C

b
 tiempo (h) Azucares MA R [-Rs] C_s C_PMA 1/R
0 65.266 0 0.42594 2.0423
13.404 46.935 7.1511 0.369013212 1.200632378 56.1005 3.57555 2.709930072
23.4854 33.6402 10.9784 0.326197506 0.775530416 40.2876 9.06475 3.065627361
37.5032 25.7842 14.6042 0.26666391 0.421392394 29.7122 12.7913 3.750038772
62.1435 19.741 18.8345 0.162016556 0.248148918 22.7626 16.71935 6.172208741
85.8429 14.3022 21.2518 0.061365204 0.285049514 17.0216 20.04315 16.29588007
99.8468 7.8561 22.5612 0.00189064 0.245472189 11.07915 21.9065 528.9213115
121.1575 7.2518 22.0576 -0.0886159 -0.11162546

MA Derivada
Azucares
f[Ra] Polynomial (Azucares)
-2.12E-03 2 MA -4.25E-03 Polynomial (MA) Lineweaver-Burk
4.26E-01
70 1 4.26E-01 4.5
1.1641 f(x) = 1.17864E-06 x⁴ − 0.000350711 x³ + 0.0380238 x² − 2.042252 x 4
60 3.5
+ 66.005164
R² = 0.995928504232054 3
50
Azucar Derivada f[-R] 2.5

1/[-r]
2
1.18E-06
40 4 4.71E-06
1.5
-3.51E-04 3 -1.05E-03
g/L

1
30
3.80E-02 2 7.60E-02 0.5
20 0
-2.04E+00 1
f(x) = − 0.002123543327511 -2.04E+00
x² + 0.425944292264448 x + 1.16413670888914 0.01 0.02 0.03 0.04 0.05 0.06 0.07 0.08 0.09 0.1
R² = 0.990893573206582
6.60E+00
10 1/C
0
0 20 40 60 80 100 120 140
tiempo (h) m 44.50360357 0.410038715
8.509881775 0.4448775
0.832894413 0.017825153 r 0.901150335 0.504297282
1.289440078 0.024821533 27.34911664 3
2.373085075 0.033656209 6.955311085 0.762947247
3.508162451 0.058748884
4.073781245 0.090259632 Vmax 2.438794103
Km 108.5351259
 1/[-Rs] 1/C_s 1/C_PMA [-Rs]/C_s C_s/[-Rs]

0.832894413 0.017825153 0.279677252 67.35607672 0.01484647

1.289440078 0.024821533 0.110317438 31.24425918 0.03200588
2.373085075 0.033656209 0.078178137 12.52049508 0.079869046
4.029838248 0.043931713 0.059810938 5.648514556 0.177037695
3.508162451 0.058748884 0.049892357 4.851998799 0.206100628
4.073781245 0.090259632 0.045648552 2.719623203 0.367697995

r-Burk Eadie-Hofstee Hanes-Woolf

1.4 0.4
1.2 0.35
1 0.3
0.8 0.25
0.2

C/-r
-r

0.6
0.15
0.4 0.1
0.2 0.05
0 0
5 0.06 0.07 0.08 0.09 0.1 0 10 20 30 40 50 60 70 80 0 10 20 30 40 50 6
/C -r/C C

b m 0.015211841 0.214128454 b m -0.00793497 0.341536598

0.000973115 0.030079207 0.001311344 0.037744028
r 0.983894529 0.054666836 r 0.948206502 0.022737537
244.3628077 4 36.61488549 2
0.730269206 0.011953852 0.018929735 0.001033991

Vmax 0.214128454 Vmax 126.0243636

Km 0.015211841 Km 43.04193243
Woolf

30 40 50 60
C

b
tiempo (h) Azucares PMA DCW Rc Cc/Rc 1/Cs
0 81.1764 0 3 2.05E-01 6.84E-02 14.61 0.012318851
12 68.0841 1.0986 5.1211 2.06E-01 4.03E-02 24.81 0.014687717
24 49.8011 3.0276 9.827 2.08E-01 2.11E-02 47.35 0.020079878
36 43.7673 4.8183 11.6263 2.09E-01 1.79E-02 55.72 0.022848108
48 42.9239 7.1625 13.0104 2.10E-01 1.61E-02 62.02 0.023297044
60 40.6271 9.23 14.1176 2.11E-01 1.49E-02 66.95 0.024614112
72 34.8009 14.7577 16.8858 2.12E-01 1.26E-02 79.66 0.028734889
84 29.5976 20.2856 19.7924 2.13E-01 1.08E-02 92.88 0.033786523
96 27.3008 26.2282 22.6989 2.14E-01 9.44E-03 105.97 0.036628963
108 25.8348 31.7562 25.3287 2.15E-01 8.50E-03 117.64 0.03870748
120 25.199 36.5918 27.6817 2.16E-01 7.82E-03 127.91 0.039684114
132 22.9022 39.4897 29.6194 2.18E-01 7.34E-03 136.17 0.043663927
144 18.1141 41.2803 32.8028 2.19E-01 6.66E-03 150.04 0.055205613
156 18.1011 42.9325 36.263 2.20E-01 6.06E-03 165.03 0.055245261
168 21.2022 45.1384 39.0311 2.21E-01 5.66E-03 176.74 0.047164917
180 21.6044 46.7906 40.4152 2.22E-01 5.49E-03 182.10 0.046286867
192 23.8752 48.858 41.3841 2.23E-01 5.39E-03 185.54 0.041884466

PMA Derivada f[Ra] Sustrato

-5.80E-04 2 -1.16E-03 Polynomial (PMA)
3.76E-01 1 3.76E-01
f(x) =803.269451452E-12 x⁶ − 4.2173715519E-09 x⁵ + 1.8100936337E-0
4.1337 R² = 0.987971220177273

70
Azucar Derivada f[-R]
3.27E-12 6 1.9617E-11
-4.22E-09 5 -2.1087E-08 60
1.81E-06 4 7.24E-06
-3.49E-04 3 -1.05E-03 50
3.37E-02 2 6.75E-02
f(x) = − 1.67045053685867E-05 x³ + 0.004636683275284 x² −
-1.85E+00 1 -1.85E+00 R² = 0.994507448750327
40 f(x) = 0.20533960375817 x + 3.14654509803922
g/L

8.22E+01 R² = 0.994844012725167

DWC Derivada rc 30
4.61E-05 2 9.23E-05
2.05E-01 1 2.05E-01 20
3.4122

10

0
0 20 40 60 80
 Lineweaver-Burk
8.00E-02

7.00E-02

6.00E-02

5.00E-02

4.00E-02

Rc
3.00E-02

2.00E-02

1.00E-02

0.00E+00
10 20 30 40 50 60
C
 Lineweaver-Burk
160.00

140.00
R² = 0.992941668712342
120.00

100.00
Cc/Rc

80.00

60.00

40.00

20.00

0.00
0.01 0.015 0.02 0.025 0.03 0.035 0.04 0.045 0.05
1/Cs

Sustrato Polynomial (Sustrato) PMA

Polynomial (PMA) DCW Linear (DCW)

E-09 x⁵ + 1.8100936337E-06 x⁴ − 0.0003490125105 x³ + 0.0337360920784 x² − 1.8480939342214 x + 82.204477318616

+ 0.004636683275284 x² − 0.031594079305699 x + 0.355814447884408

654509803922

60 80 100 120 140 160 180 200

tiempo (h)
weaver-Burk Ra [-Rs] C_s C_PMA

0.36233288 1.177129287 74.63025 0.5493

0.34841576 0.738609948 58.9426 2.0631
0.33449864 0.472482675 46.7842 3.92295
0.32058152 0.328010882 43.3456 5.9904
0.3066644 0.263194941 41.7755 8.19625
0.29274728 0.244186423 37.714 11.99385
0.27883016 0.244702338 32.19925 17.52165
0.26491304 0.245439375 28.4492 23.2569
0.25099592 0.233488136 26.5678 28.9922
0.2370788 0.201747386 25.5169 34.174
0.22316168 0.148338281 24.0506 38.04075
40 50 60 70 80 90 0.20924456 0.076018616 20.50815 40.385
C 0.19532744 -0.00840294 18.1076 42.1064
0.18141032 -0.0946526 19.65165 44.03545
0.1674932 -0.16957723 21.4033 45.9645

Lineweaver-Burk
14
12
10
8
1/[-r]

6
4
2
0
0.015 0.02 0.025 0.03 0.035 0.04 0.045 0.05 0.055
1/C

m 268.7394723 -3.73357719 b
56.3405583 1.846241904
r 0.716553778 1.760676264
22.75205492 9
70.53093584 27.89982816

Vmax 0.267839648
Km 71.97908558
Cc*Cs/Rc Cs
1185.98 81.1764
1688.89 68.0841 Hanes-Woolf
2357.92 49.8011 3500.00
2438.65 43.7673
3000.00
2662.26 42.9239 R² = 0.932934056365958
2719.89 40.6271 2500.00
2772.13 34.8009
2749.12 29.5976 2000.00

Cc*Cs/rc
2893.14 27.3008
1500.00
3039.26 25.8348
3223.29 25.199 1000.00
3118.60 22.9022
500.00
2717.88 18.1141
2987.29 18.1011 0.00
20 30 40 50 60 70
3747.30 21.2022
3934.07 21.6044 Cc
4429.70 23.8752

Lineweaver-Burk
m 3843.623716 124.1558281 Km
b -30.9580611 0.032301764 mu max

Hanes-Woolf
m -28.9679567 -0.0345209 mu max
b 123.7572807 -4.27221299 Km

Eadie-Hofstee
m 116.0757382 Km
b 0.00025005 mu max
 1/R 1/[-Rs] 1/C_s 1/C_PMA [-Rs]/C_s C_s/[-Rs]

2.759893057 0.849524356 0.013399392 1.820498817 87.84945301 0.01138311

2.870134233 1.353894573 0.016965658 0.484707479 43.53559073 0.022969712
2.989548777 2.11647972 0.021374738 0.254910208 22.10472397 0.045239199
3.119331395 3.048679343 0.023070392 0.166933761 14.21782848 0.070334229
3.260893667 3.79946513 0.023937475 0.122007015 10.99510025 0.090949603
3.415915598 4.09523178 0.026515352 0.083376064 9.20924676 0.108586514
3.586412603 4.086597644 0.031056624 0.057072251 7.879231773 0.126915926
3.774823618 4.074325897 0.035150373 0.042997992 6.982553855 0.143214076
3.984128507 4.282872851 0.037639549 0.034492036 6.203266108 0.161205401
4.21800684 4.956693724 0.039189713 0.029262012 5.147967864 0.194251407
4.481056067 6.741348188 0.041579004 0.026287599 3.567624654 0.280298545
4.779096766 13.15467254 0.048761102 0.024761669 1.559001179 0.641436333
5.119608387 -119.005982 0.05522543 0.023749359 -0.15215706 -6.57215657
5.512365559 -10.5649502 0.050886312 0.022708977 -1.86007975 -0.53761136
5.970391634 -5.89701828 0.046721767 0.02175592 -3.62951223 -0.27551912

Eadie-Hofstee
0.8 0.25
0.7
0.6 0.2
0.5 0.15

C/-r
0.4
-r

0.3 0.1
0.2 0.05
0.1
0 0
0.045 0.05 0.055 0 5 10 15 20 25 30 35 40 45 50 20 30

-r/C

m 0.01486211 0.113027816 b m
0.000770692 0.012691013
r 0.976370308 0.028971372 r
371.8767356 9
0.312131172 0.007554064

Vmax 0.113027816
Km 0.01486211
 Rc/Cc Rc/Cc*Cs
0.07 0.000843184
0.04 0.000592106 Eadie-Hofste
0.02 0.000424103 0.08
0.02 0.000410062
0.07
0.02 0.000375621 R² = 0.996930785944292
0.01 0.000367662 0.06
0.01 0.000360734 0.05
0.01 0.000345646 0.010696993 0.000361414

Rc/Cc
0.01 0.000329027 0.04

0.01 0.000310242 0.03

0.01 0.000320656
0.02
0.01 0.000343919 0.006949069 0.000303424
0.01 0.000309082 0.006229782
0.01 0.000343919
0.01 0.000266859 0.005594719 0.000309082
70 80 0.00
0.01 0.00025419 0.0002 0.0003 0.0004 0.0005
0.01 0.000225749 Rc/Cc*C
 Hanes-Woolf
0.25

0.2
0.15
C/-r

0.1
0.05

0
20 30 40 50 60 70 80
C

-0.00361513 0.247867437 b
0.000504993 0.022289667
0.864974719 0.023593312
51.24816405 8
0.028527003 0.004453155

Vmax 276.6150115
Km 68.56385393
 Eadie-Hofstee

.996930785944292

0.0004 0.0005 0.0006 0.0007 0.0008 0.0009

Rc/Cc*Cs
tiempo (h) Glucosa PMA DCW Rc mu
0 140.0 0.0 1.0 0.2467 0.2467
12 116.9 1.4 1.8 0.2201 0.12296775
24 107.5 5.0 4.2 0.1967 0.047386286 140.0

36 102.0 9.7 6.8 0.1763 0.026006261

48 92.4 13.6 10.0 0.1591 0.015975939
60 88.8 17.4 11.8 0.1450 0.012333912 120.0
72 81.4 22.4 13.8 0.1341 0.009689436
84 75.3 25.7 14.4 0.1263 0.008775909
96 64.3 28.2 15.9 0.1216 0.007642829 100.0
108 61.0 32.7 17.7 0.1200 0.00677798
120 55.9 34.7 18.3 0.1216 0.006659759
132 49.0 36.8 19.9 0.1263 0.006337427 80.0
144 41.0 41.8 21.3 0.1341 0.006294283
156 37.8 45.0 23.7 0.1451 0.00613212

g/L
168 34.9 47.1 25.5 0.1592 0.00625174
60.0
180 30.7 48.3 28.4 0.1764 0.006216362
192 25.2 51.9 29.1 0.1967 0.006769499
mu
40.0
Cp Derivada f[Ra]
-5.80E-04 2 -1.16E-03
3.76E-01 1 3.76E-01 f(x) = 3.62077757313127E
20.0 R² = 0.99419713767568
4.1337 0 0

Cs Derivada f[-R]
3.39E-11 6 2.03442E-10 0.0
0 20
-2.39E-08 5 -1.1974E-07
6.56E-06 4 2.63E-05
-8.72E-04 3 -2.61E-03
5.78E-02 2 1.16E-01
-2.31E+00 1 -2.31E+00
1.39E+02 0 0

Cc Derivada rc
3.62E-06 3 1.09E-05
-1.17E-03 2 -2.35E-03
2.47E-01 1 2.47E-01
-1.32E-01 0 0.00
 Sustrato PMA DCW Polynomial (DCW)

f(x) = 3.62077757313127E-06 x³ − 0.001173034826138 x² + 0.246669409328059 x − 0.132053663570689

R² = 0.99419713767568

20 40 60 80 100 120 140 160 180 200

tiempo (h)
180 200
tiempo (h) Glucosa PMA Biomasa Rc mu

0 190 0 2.8 2.429 0.868

12 150 9.5 29 1.953 0.067
24 83 25 50 1.477 0.030
1.00
36 47 35 64 1.001 0.016
0.90
48 17 45 74 0.524 0.007
0.80
60 5 47 77 0.048 0.001
0.70
x
0.60
0.50
tiempo (h) Glucosa PMA Biomasa Rc mu 0.40
0 190 0 0 -1.089 #DIV/0! 0.30
12 190 0 2.8 1.38868896 0.495960343 0.20
24 150 9.5 29 2.09244768 0.072153368 0.10
36 83 25 50 1.69951392 0.033990278 0.00
0 20 40 60
48 47 35 64 0.88712544 0.013861335
60 17 45 74 0.33252 0.004493514
72 5 47 77 0.71293536 0.009258901

MA Derivada f[Ra]
-2.12E-03 2 -4.25E-03
4.26E-01 1 4.26E-01
1.1641

Azucar Derivada f[-R]

-3.09E-05 4 -1.23E-04
5.91E-03 3 1.77E-02
-3.51E-01 2 -7.02E-01
3.61E+00 1 3.61E+00
1.90E+02

Biomasa Derivada rc
0.00E+00 4 0.00E+00
0.00E+00 3 0.00E+00
-1.98E-02 2 -3.97E-02 Column B Po
Polynomial (Column C) Co
2.429 1 2.43E+00
2.80E+00 0 0.00E+00 200
f(x) = 0.035714285714286 x² − 5.38095238095238 x + 196.285714285714
R² = 0.990685474710341
180

160

-1.98E-02
140
2.43E+00
2.80E+00
120

100
g/L

80
f(x) = − 0.01984126984127 x² + 2.42857142857143 x + 2.799999999
 140

120

100

g/L
80
f(x) = − 0.01984126984127 x² + 2.42857142857143 x + 2.799999999
R² = 0.999876614860141
60

40 f(x) = − 0.000286672668038 x³ + 0.018422067901235 x² + 0.7140652557319

R² = 0.997740285998786

20

0
0 10 20 30
 Chart Title

0 20 40 60 80 100 120 140 160 180 200

Column F

Chart Title
70

60

50

40
0.832894413 0.017825153 30
1.289440078 0.024821533
20
2.373085075 0.033656209
3.508162451 0.058748884 10
4.073781245 0.090259632 0
0.000 0.500 1.000 1.500 2.000 2.500 3.000

Column A

Polynomial (Column B) Column C

(Column C) Column D Polynomial (Column D)

238 x + 196.285714285714

2857143 x + 2.79999999999999
2857143 x + 2.79999999999999

901235 x² + 0.714065255731921 x − 0.246031746031737

0 30 40 50 60 70

tiempo (h)
 R [-Rs] C_s C_PMA
0.42594 -3.6131
0.42594 -3.6131 95 0
0.374976 2.476461664 170 4.75
0.324012 4.741575712 116.5 17.25
0.273048 4.462565728 65 30
0.222084 2.919755296 32 40
0.17112 1.393468

Lineweaver-Burk
0.5
0.4
0.3
0.2
1/[-r]

0.1

-r
0 -400
-0.1 0 0.005 0.01 0.015 0.02 0.025 0.03 0.035
-0.2
-0.3
2.000 2.500 3.000
-0.4
1/C

m 6.06811995 0.093974272 b
14.96846709 0.253346251
r 0.051936177 0.30169885
0.164343927 3
0.014958945 0.273066589

Vmax 10.64121042
Km 64.57214123

Column B Column C Column D

200

180

160
 Column B Column C Column D
200

180

160

140

120

g/L 100

80

60

40

20

0
-5 5 15 25 35 45

tiempo (h)
 1/R 1/[-Rs] 1/C_s 1/C_PMA [-Rs]/C_s C_s/[-Rs]

2.347748509 -0.27677064 0.010526316 #DIV/0! -343.2445 -0.00291338

2.666837344 0.40380193 0.005882353 0.210526316 420.9984829 0.002375305
3.086305445 0.210900355 0.008583691 0.057971014 552.3935704 0.001810303
3.662359732 0.224086335 0.015384615 0.033333333 290.0667723 0.003447482
4.502800742 0.342494455 0.03125 0.025 93.43216947 0.010702952

Eadie-Hofstee Hanes-Woolf
6 0.012
4 0.01
0.008
2
0.006
C/-r

0 0.004
-r

-400 -200 0 200 400 600 800

-2 0.002
0
-4 20 40 60 80 100 120 140 160 180
-0.002
-6 -0.004
-r/C C

m 0.008699883 0.433730555 b m 5.98123E-06 0.000512275 b

0.002489106 0.926712069 4.47081E-05 0.0052768
r 0.802843176 1.738043924 r 0.008869688 0.003428762
12.21631327 3 0.017898127 2
36.9029986 9.062390048 2.10418E-07 2.35128E-05

Vmax 0.433730555 Vmax -167189.831

Km 0.008699883 Km -85.6471251

C Column D
C Column D

45 55 65 75

h)
0 160 180
tiempo (h) glicerol MA R [-Rs] C_s C_PMA
0 104.9284 0 0.42594 -0.0095735
23.8272 100.0752 3.8909 0.324745882 0.239735354 102.5018 1.94545
48.1793 93.8239 9.5314 0.221322513 0.370703716 96.94955 6.71115
72.0153 85.8228 15.1713 0.120091021 0.419339214 89.82335 12.35135
96.3416 73.9752 24.3096 0.016777225 0.430627073 79.899 19.74045
120.1583 61.7769 37.2943 -0.0843723 0.445728236 67.87605 30.80195
144.4926 51.6782 51.3284 -0.18772007 0.508080347 56.72755 44.31135
168.3109 39.8296 71.6592 -0.28887639 0.656624537 45.7539 61.4938
192 19.2361 83.5946 -0.389484 0.930969066
MA Derivada Glic f[Ra] Polynomial (Glic)
-2.12E-03 2 MA -4.25E-03 Lineweaver-Burk
4.26E-01
120 1 4.26E-01 4.5
1.1641 4
100 f(x) = − 1.25952E-07 x⁴ + 4.72744E-05 x³ − 0.00677822 x² + 0.00957349 x 3.5
3
+ 104.40516
Azucar Derivada f[-R] 2.5

1/[-r]
80 R² = 0.998525853829837
2
-1.26E-07 4 -5.04E-07
1.5
60
4.73E-05 3 1.42E-04
g/L

1
-6.78E-03 2 -1.36E-02 0.5
40 0
9.57E-03 1 9.57E-03 0.008 0.01 0.012 0.014 0.016 0.018 0.02 0.022 0.024
1.04E+02
20 1/C

0
0 20 40 60 80 100 120 140 160 180 200
tiempo (h) m -144.946116 4.500838427
53.93342496 0.786247547
r 0.590923738 0.584460235
7.222659835 5
2.467215573 1.70796883

Vmax 0.222180826
Km -32.2042477
 1/R 1/[-Rs] 1/C_s 1/C_PMA [-Rs]/C_s C_s/[-Rs]

3.079330814 4.171266295 0.009755926 0.514019893 24.57330526 0.040694566

4.518293177 2.697572092 0.010314643 0.149005759 35.93955849 0.027824493
8.327017228 2.384704234 0.011132963 0.08096281 37.66645302 0.026548823
59.60461351 2.322194918 0.012515801 0.050657406 34.40667249 0.02906413
-11.8522311 2.243519523 0.014732737 0.032465477 30.25427205 0.033053183
-5.32708084 1.968192639 0.017628119 0.022567581 28.82215331 0.034695534
-3.46168821 1.522940346 0.02185606 0.016261802 30.04313342 0.033285476

r-Burk Eadie-Hofstee Hanes-Woolf

0.7 0.045
0.6 0.04
0.035
0.5
0.03
0.4 0.025

C/-r
-r

0.3 0.02
0.2 0.015
0.01
0.1 0.005
0 0
016 0.018 0.02 0.022 0.024 20 22 24 26 28 30 32 34 36 38 40 45 55 65 75 85 95
/C -r/C C

b m 0.003376754 0.331741918 b m -1.6530547E-05

0.0123764 0.395456149 0.00010216096
r 0.014669723 0.138261737 r 0.00520914292
0.074440639 5 0.02618210092
0.00142303 0.095581539 7.35521911E-07

Vmax 0.331741918 Vmax

Km 0.003376754 Km
 Hanes-Woolf

55 65 75 85 95 105
C

0.033440707 b
0.008124987
0.00530024
5
0.000140463

60494.06619
2022.964367
tiempo (h) Cs (g/l) Cp (g/l) Cc (g/l) rc mu
0 140.00 0.00 1.00 0.2467 0.2467
12 1.38 1.79 0.2201
24 107.54 5.00 4.15 0.1967 0.0474
36 102.00 9.68 6.78 0.1763 0.0260
48 92.36 13.56 9.96 0.1591 0.0160
60 88.81 17.44 11.76 0.1450 0.0123
72 81.38 22.42 13.84 0.1341 0.0097
84 75.25 25.74 14.39 0.1263 0.0088
96 64.26 28.24 15.91 0.1216 0.0076
108 61.00 32.66 17.71 0.1200 0.0068
120 55.87 34.74 18.26 0.1216 0.0067
132 49.00 36.81 19.93 0.1263 0.0063
144 41.00 41.79 21.31 0.1341 0.0063
156 37.78 44.98 23.66 0.1451 0.0061
168 34.89 47.06 25.46 0.1592 0.0063
180 30.68 48.30 28.37 0.1764 0.0062
192 25.19 51.90 29.06 0.1967 0.0068

Cs (g/l)
Cc (g/l)
160.00

140.00

120.00

3.621E-06 3 1.086E-05 100.00

-1.173E-03 2 -2.346E-03
80.00
Cs

2.467E-01 1 2.467E-01 f(x) = 3.62077757313127E-06 x³ − 0.001173034826

R² = 0.99419713767568
-1.321E-01 0 -0.000E+00 60.00

40.00

20.00

0.00
0 20 40 60 80 1
t(
 mu Vs Cs
0.30

0.25
f(x) = 4.79210369868591E-07 x³ − 8.32420378946269E-05 x² + 0.004492615062363 x
− 0.067210353769391
0.20 R² = 0.996346300985968

0.15
mu

0.10

0.05

0.00
0.00 20.00 40.00 60.00 80.00 100.00 120.00 140.00 160.00
Cs

Cs (g/l) Cp (g/l)
Cc (g/l) Polynomial (Cc (g/l))
60.00

50.00

40.00
Cc,Cp

30.00
77757313127E-06 x³ − 0.001173034826138 x² + 0.246669409328059 x − 0.132053663570689
9713767568
20.00

10.00

0.00
20 40 60 80 100 120 140 160 180 200
t(h)