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1. Cytotoxic effect of methanol extracts and partitions of two mexican desert plants against the murine
lymphoma L5178Y-R
By Quintanilla-Licea, Ramiro; Gomez-Flores, Ricardo; Angel, Samaniego-Escamilla Mario; Carlos, Hernandez-
Martinez Humberto; Tamez-Guerra, Patricia; Morado-Castillo, Rolando
From American Journal of Plant Sciences (2016), 7(11), 1521-1530. Language: English, Database: CAPLUS,
DOI:10.4236/ajps.2016.711143
Pachycereus marginatus (DC.) Britton & Rose and Ibervillea sonorae (S. Watson) Greene have been used in the
Mexican traditional medicine for the treatment of various diseases, including cancer. The present study aims to
investigate the cytotoxic activity of these plants against a murine lymphoma. Soxhlet extn. of dried and powd. plant
material was performed with methanol. Also, a further partitioning of these methanolic exts. with hexane and Et acetate
was achieved. The in vitro cytotoxic activity against the murine lymphoma L5178Y-R cell line was assessed via the
colorimetric MTT assay. The methanol ext. from P. marginatus exhibited high cytotoxic activity (up to 94% ) at concns.
ranging from 3.9 to 500 µg/mL; however, hexane and Et acetate partitions from this methanolic ext. showed lower but
significant (p < 0.05) concn.-dependent cytotoxicity (hexane partition up to 94%at 500 µg/mL; Et acetate partition up to
94%at 65.5 µg/mL). The methanolic ext. and partitions derived from I. sonorae also showed significant (p < 0.05) and
concn.-dependent cytotoxicity against L5178Y-R cells at concns. ranging from 7.81 to 500 µg/mL (methanolic ext. up to
63%at 500 µg/mL; hexane partition up to 76%at 250 µg/mL; Et acetate partition up to 73%at 500 µg/mL). These results
demonstrate that the methanol exts. and partitions from P. marginatus and I. sonorae possess significant cytotoxic
activity against the murine lymphoma L5178Y-R and validate the ethnobotanical use of these plants for the treatment of
diseases consistent with cancer symptomatol. Previous scientific reports describe the isolation of isoquinoline alkaloids
of P. marginatus as well as cucurbitacins from I. sonorae, phytochems. that could be responsible for their obsd. cytotoxic
activity in this research. The direct extn. with methanol of medicinal plants allows extg. of both high and low-polarity
compds., contrary to the simple extn. with water that only allows obtaining compds. of high polarity. The subsequent
partition of the methanol ext. with a solvent of low polarity (hexane) and another of medium polarity (Et acetate) allows
making a preliminary fractionation of the bioactive mols. present in the plant that will facilitate the bioguided chromatog.
isolation of the pure compds. responsible for the biol. activity of the plant.
~0 Citings
Copyright © 2017 American Chemical Society (ACS). All Rights Reserved.

2. Antiproliferative and apoptosis induction of cucurbitacin-type triterpenes from Ibervillea sonorae

By Torres-Moreno, H.; Velazquez, C. A.; Garibay-Escobar, A.; Curini, Massimo; Marcotullio, M. C.; Robles-Zepeda, R.
E.
From Industrial Crops and Products (2015), 77, 895-900. Language: English, Database: CAPLUS,
DOI:10.1016/j.indcrop.2015.09.055
Two cucurbitacin-type triterpenes kinoin A (1) and kinoin B diglucoside (2) were isolated and characterized by
chromatog. and spectroscopic techniques from the root of Ibervillea sonorae. By MTT assay their antiproliferative activity
was evaluated and only compd. 1 was able to inhibit the growth of HeLa, M12AK.C3F6, A549 and RAW 264.7 cancer cell
lines with a IC50 of 18.0, 31.4, 40.2, and 61.5 µM, resp. L-929 cell line used as a control was more resistant to compd. 1
at IC50 values greater than 100 µM. Apoptosis induction was quantified for a double staining by Annexin V/propidium
iodide using flow cytometry, and it was obsd. that treatment of HeLa cells with compd. 1 cause cell death by apoptosis.
~0 Citings

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3. Chemical constituents from Ibervillea lindheimeri (A. Gray) Greene

By Figueroa-Hernandez, J. L.; Martinez-Vazquez, M.
From Biochemical Systematics and Ecology (2014), 54, 237-239. Language: English, Database: CAPLUS,
DOI:10.1016/j.bse.2014.01.004
A chem. anal. of Ibervillea lindheimeri (A. Gray) Greene led to the isolation of 23,24-dihydrocucurbitacin F (1), 23,24-
dihydrocucurbitacin D (2) and 2-O-β-D-glucopyranosyl 23,24-dihydrocucurbitacin D (3). Their structures were identified
by spectroscopic analyses and by comparison of their spectral data with those reported in the literature. In addn., 10
compds. were detected by GC-MS analyses from the hexane ext. To the best of our knowledge, this is the first report
examg. the chem. compn. of I. lindheimeri.
~0 Citings

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4. Isolation of a new anti-inflammatory 20, 21, 22, 23, 24, 25, 26, 27-octanorcucurbitacin-type triterpene from
Ibervillea sonorae
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By Jardon-Delgado, Angel; Magos-Guerrero, Gil Alfonso; Martinez-Vazquez, Mariano
From Natural Product Communications (2014), 9(1), 15-16. Language: English, Database: CAPLUS
A new cucurbitane-type triterpene, 20, 21, 22, 23, 24, 25, 26, 27-octanorcucurbita-5-ene-3, 11, 16-trione (1), named
kinoin D, was isolated from the roots of the medicinal plant Ibervillea sonorae, (wereque). The structure of 1 was
established on the basis of extensive NMR and MS studies. In addn., the known kinoins B (3) and C (5) were isolated,
as were 16α-20,25-trihydroxy-3α-(2-O-α-L-rhamnopyranosiyl-D-glucopyranosyloxy)-(10α)-cucurbit-5-en-11,22-dione (6),
(22S)-16α,22-diacetoxy-20,25-dihydroxy-3α-[3,4,6-tri-O-acetyl-2-O-(2,3,4-tri-O-acetyl-α-L-rhamnopyranosyl)-β-
glucopyranosyl]-(10α)-cucurbita-5,23t-dien-11-one (7) and 16α-acetoxy-20,25-dihydroxy-3α-[3,4,6-tri-O-acetyl-2-O-
(2,3,4,-tri-O-acetyl-α-L-rhamnopyranosyl)-β-D-glucopyranosyl]-(10α)-cucurbita-5-ene-11,22-dione (8). Compd. 1
exhibited anti-inflammatory activity in TPA-induced edema in mice.
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5. Identification of the main Wereque's root components, extractible in aqueous medium, by GC-MS
By Morales, Dulce M.; Siles, Samuel
From Avances en Ciencias e Ingenieria (2013), 4(2), 15-21. Language: English, Database: CAPLUS
This work comprises the identification of the Wereque's root main components. For that purpose, aq. extns. of
Wereque's fresh roots were made by infusion, decoction (both neutral and basic), and maceration, from which six
samples were obtained for anal. The raw exts. were filtered, purified and derivatized before they were analyzed. The
GC-MS analyses were conducted on a Perkin Elmer Clarus 600T instrument, an Elite-5 MS, 30 m capillary column, ϕint:
0.32 mm, film: 0.25 µm; He: 0.8 mL/min; Tinj: 200 °C; Tsource and Ttransferline: 230 °C; Tcolumn: 60 °C, 5min; 60 a 180°C,
20°C/min; 180°C, 10min; 70 eV, m/z 30 a 450. Through this work, 33 different compds. were identified, including palmitic
acid. Based on existing literature, this component belongs to a group of fatty acids and monoglycerides responsible of
the Wereque's hypoglycemic activity.
~0 Citings

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6. Comparing DNA Extraction Methods for Analysis of Botanical Materials Found in Anti-Diabetic Supplements
By Llongueras, Jose P.; Nair, Saraswathy; Salas-Leiva, Dayana; Schwarzbach, Andrea E.
From Molecular Biotechnology (2013), 53(3), 249-256. Language: English, Database: CAPLUS, DOI:10.1007/s12033-
012-9520-0
A comparative performance evaluation of DNA extn. methods from anti-diabetic botanical supplements using various
com. kits was conducted, to det. which produces the best quality DNA suitable for PCR amplification, sequencing and
species identification. All plant materials involved were of suboptimal quality showing various levels of degrdn. and
therefore representing real conditions for testing herbal supplements. Eight different DNA extn. methods were used to
isolate genomic DNA from 13 medicinal plant products. Two methods for evaluation, DNA concn. measurements that
included absorbance ratios as well as PCR amplifiability, were used to det. quantity and quality of extd. DNA. We found
that neither DNA concns. nor commonly used UV absorbance ratio measurements at A 260/A 280 between 1.7 and 1.9 are
suitable for globally predicting PCR success in these plant samples, and that PCR amplifiablity itself was the best
indicator of extd. product quality. However, our results suggest that A 260/A 280 ratios below about 1.3 and above 2.3
indicated a DNA quality too poor to amplify. Therefore, A 260/A 280 measurements are not useful to identify samples that
likely will amplify but can be used to exclude samples that likely will not amplify reducing the cost for unnecessarily
subjecting samples to PCR. The two Nucleospin plant II kit extn. methods produced the most pure and amplifiable
genomic DNA exts. Our results suggest that there are clear, discernable differences between extn. methods for low
quality plant samples in terms of producing contamination-free, high-quality genomic DNA to be used for further anal.
~2 Citings

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7. Influence of two hypoglycemic cucurbitaceae (Cucurbita ficifolia Bouche and Ibervillea sonorae Greene) on
ATP-sensitive potassium channels in rat aortic rings
By Banderas-Dorantes, Tania Rosario; Roman-Ramos, Ruben; Zamilpa, Alejandro; Garcia-Macedo, Rebeca; Diaz,
Margarita; Campos, Maria Guadalupe; Tortoriello, Jaime; Alarcon-Aguilar, Francisco Javier
From Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas (2012), 11(6), 510-519. Language:
English, Database: CAPLUS
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Cucurbita ficifolia Bouche fruit contg. D-chiro-inositol and Ibervillea sonorae Greene root contg. cucurbitane-type
glycosides are used to control diabetes in Mexico. Although the hypoglycemic effect of both plants has been
demonstrated and some active compds. proposed, their mechanisms are still unknown. The aim of this study was to det.
if the incubation with both aq. exts. avoids the inhibition of contraction induced by phenylephrine similarly to
glibenclamide in rat aortic rings. The hypoglycemic aq. exts. of C. ficifolia and I. sonorae were characterized for their
content of either D-chiro inositol or cucurbitanes resp., and then we assayed the characterized exts. in vitro on the
diazoxide-induced relaxation of rat aortic rings precontracted with phenylephrine, using as pos. control glibenclamide. I.
sonorae ext. blocked the KATP channels in a concn.-dependent manner (p < 0.05), whereas C. ficifolia ext. had no effect
on these channels. I. sonorae ext. produces a hypoglycemic effect through a similar mechanism to sulfonylureas in this
exptl. model; however, hypoglycemic action of C. ficifolia ext. should be explained by an independent KATP channels
mechanism.
~0 Citings

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8. Fatty acids, phenols content, and antioxidant activity in Ibervillea sonorae callus cultures
By Estrada-Zuniga, M. E.; Arano-Varela, H.; Buendia-Gonzalez, L.; Orozco-Villafuerte, J.
From Revista Mexicana de Ingenieria Quimica (2012), 11(1), 89-96. Language: English, Database: CAPLUS
Ibervillea sonorae callus cultures were established in order to produce fatty acids (lauric, myristic, pentadecanoic,
palmitic and stearic acids) and phenolic compds. Highest callus induction (100%) was obtained in treatments contg. 2.32
or 4.65 µM Kinetin (KIN) with 2.26 or 6.80 µM 2,4-Dichlorophenoxyacetic acid (2,4-D). Highest fatty acids (FA) prodn.
(48.57 mg g-1), highest total phenol content (TPC; 57.1 mg gallic acid equiv. [GAE] g-1) and highest antioxidant activity
(EC50; 573.3-855.7 µg mL-1) was obtained from callus derived from 4.65 µM KIN with 6.80 µM 2,4-D. A direct
relationship was obsd. between the callus growth index (GI), FA content, TPC and EC50. FA content was significantly
higher in callus (48.57 mg g-1) than the reported for tuber roots from wild plant (0.25 mg g-1). TPC and EC50 were higher
in leaves (360.1 mg GAE g-1 and 66.0-75.5 µg mL-1, resp.) than in callus.
~2 Citings

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9. Antiobesity and hypoglycaemic effects of aqueous extract of Ibervillea sonorae in mice fed a high-fat diet with
fructose
By Rivera-Ramirez, Fabiola; Escalona-Cardoso, Gerardo N.; Garduno-Siciliano, Leticia; Galaviz-Hernandez, Carlos;
Paniagua-Castro, Norma
From Journal of Biomedicine and Biotechnology (2011), 968984, 6 pp.. Language: English, Database: CAPLUS,
DOI:10.1155/2011/968984
Obesity, type II diabetes, and hyperlipidemia, which frequently coexist and are strongly assocd. with oxidative stress,
increase the risk of cardiovascular disease. An increase in carbohydrate intake, esp. of fructose, and a high-fat diet are
both factors that contribute to the development of these metabolic disorders. In recent studies carried out in diabetic rats,
authors reported that Ibervillea sonorae had hypoglycemic activity; saponins and monoglycerides present in the plant
could be responsible for the effects obsd. In the present study, we detd. the effects of an aq. I. sonorae ext. on a murine
model of obesity and hyperglycemia, induced by a high-calorie diet, and the relationship of these effects with hepatic
oxidn. A high-fat diet over a period of 8 wk induced wt. gain in the mice and increased triglycerides and blood glucose
levels. Simultaneous treatment with I. sonorae aq. exts., at doses of 100, 200, and 400 mg/kg, decreased triglycerides
and glycemia levels, prevented an increase in body wt. in a dose-dependent manner, and decreased hepatic lipid oxidn.
at a dose of 200 mg/kg. These data suggest that the aq. ext. from I. sonorae root prevents obesity, dyslipidemia, and
hyperglycemia induced by a hypercaloric diet; however, high doses may induce toxicity.
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10. Nutritional supplement for the prevention of cardiovascular disease, Alzheimer's disease, diabetes, and
regulation and reduction of blood sugar and insulin resistance
By Mazed, Mohammad A.; Mazed, Sayeeda
From U.S. Pat. Appl. Publ. (2010), US 20100021533 A1 20100128, Language: English, Database: CAPLUS
 SciFinder® Page 4
A synergistic mixt. (which may be utilized as a food or a drink or a supplement or a drug or a cosmetic or a hygienic
product) that is formulated and is capable of improving a person's well being, lowering the risks of cardiovascular and/or
Alzheimer's diseases and/or lowering blood sugar using natural and synthetic ingredients is described. Numerous ratios
may be formulated for aroma, color, flavor, flow (viscosity), taste and uniformity. Moreover, ingredients for sugar
substitutes, natural preservatives, nano-dispersion, nano-emulsion, nano-encapsulation of ingredients and app. for
personalized nutrition are also described herein. A mixt. having a deep maroon-red color and a faint fruity flavor was
prepd. from some essential micronutrients and various plants high in super nutrients, antioxidants and anti-degenerative
elements, such as acaiberry, gojiberry, Mangosteen, black currant, black carrot, pomegranate, blueberry, red tart cherry,
bitter melon, curcumin ext., vitamins, minerals, etc.
~18 Citings

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11. Duplication and paralog sorting of RPB2 and RPB1 genes in core eudicots
By Luo, Jie; Yoshikawa, Nori; Hodson, Matthew C.; Hall, Benjamin D.
From Molecular Phylogenetics and Evolution (2007), 44(2), 850-862. Language: English, Database: CAPLUS,
DOI:10.1016/j.ympev.2006.11.020
RPB1 and RPB2, which encode the largest and second largest subunits of RNA polymerase II, resp., are essential single
copy genes in fungi, animals and most plants. Two paralogs of the RPB2 gene have been found in some groups of
angioperms [RPB2 gene phylogeny in flowering plants, with particular emphasis on asterids. Mol. Phylogenet. Evol. 32,
462-479]. Here, we report the results of expts. designed to identify the evolutionary origin of the RPB2 duplicate copies.
Through careful sampling and phylogenetic anal., we were able to construct the RPB2 gene tree in angiosperms and
infer the phylogenetic positions of the gene duplication and gene loss events that occurred. Our study shows that an
RPB2 gene duplication occurred early in core eudicot evolution, at or near the time of the Buxaceae/Trochodendraceae
divergence. Subsequently, multiple gene duplication and paralog sorting events happened independently in different
core eudicot taxa. Differential expression of the two RPB2 gene paralogs may explain the preservation of both paralogs
in the asterids. One gene (RPB2-i) accounts for most of the RPB2 mRNA made in the flower organs while the other
gene (RPB2-d) is predominantly used in the vegetative tissues. We also found two paralogs of the RPB1 gene in some
core eudicot species. The RPB1 gene duplication occurred before core eudicot divergence, around the time of RPB2
gene duplication. Several independent RPB1 paralog sorting events happened in different core eudicot taxa; their
occurrence was independent of the RPB2 paralog sorting events. Our results suggest that a polyploidization event
happened at or near the time of the Buxaceae/Trochodendraceae divergence. We propose that this polyploidization and
the partial diploidization processes thereafter may have been the driving force of core eudicot radiation.
~6 Citings

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12. Monoglycerides and fatty acids from Ibervillea sonorae root: isolation and hypoglycemic activity
By Hernandez-Galicia, Erica; Calzada, Fernando; Roman-Ramos, Ruben; Alarcon-Aguilar, Francisco J.
From Planta Medica (2007), 73(3), 236-240. Language: English, Database: CAPLUS, DOI:10.1055/s-2007-967117
Eleven monoglycerides (MG), 1-monopalmitin (1), glyceryl 1-monomargarate (2), 1-monostearin (3), glyceryl 1-
monononadecylate (4), glyceryl 1-monoarachidate (5), glyceryl 1-monobehenate (6), glyceryl 1-monotricosanoate (7),
glyceryl 1-monotetracosanoate (8), glyceryl 1-monopentacosanoate (9), glyceryl 1-monohexacosanoate (10) and glyceryl
1-monooctacosanoate (11), together with five fatty acids (FA), lauric acid (12), myristic acid (13), pentadecanoic acid
(14), palmitic acid (15) and stearic acid (16) were isolated of the root of Ibervillea sonorae Greene (Cucurbitaceae). Their
structures were detd. by spectroscopic and chem. methods as well as GC-MS anal. The hypoglycemic activity of the
dichloromethane (DCM) ext., of fractions (F1-F10 and SF1-SF5), of monoglycerides (MG) and of fatty acids (FA) mixts.
obtained of the root from I. sonorae was evaluated in normoglycemic and alloxan-induced diabetic mice. The results
showed that by i.p. administration the DCM ext. (300 mg/kg), F9 (300 mg/kg) and SF1 (150 mg/kg) significantly reduced
glucose levels in both models. For fraction SF1, the hypoglycemic activity was more pronounced than that of tolbutamide
(150 mg/kg) used as control. However, neither MG (75 mg/kg) nor FA (75 mg/kg) mixts. isolated from SF1 exhibited a
significant hypoglycemic effect. However, when MG and FA were combined in equal proportions (75 mg: 75 mg/kg),
their effect was comparable to that of SF1. The obsd. activity for the DCM ext., F9, SF1 and the MG-FA mixt. provides
addnl. support for the popular use of this plant in the treatment of diabetes mellitus in Mexican traditional medicine.
~8 Citings

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13. Constituents of tropical medicinal plants. Part 58. The absolute configuration of kinoin C
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By Weckert, Edgar; Huemmer, Kurt; Dominguez, Xorge A.; Horn, Konrad; Achenbach, Hans
From Phytochemistry (1993), 33(2), 447-8. Language: English, Database: CAPLUS, DOI:10.1016/0031-
9422(93)85536-Z
The abs. configuration of kinoin C (I), a novel minor constituent from Ibervillea sonorae and degrdn. product of its major
constituents kinoin A glycoside and kinoin B glycoside, has been established to be 3R,8S,9R,10R,13R,14S,16R,17R.

~0 Citings

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14. Constituents of tropical medicinal plants. Part 55. Cucurbitanes and cucurbitane-type glycosides from
Ibervillea sonorae
By Achenbach, Hans; Horn, Konrad; Dominguez, Xorge A.; Rombold, Carlos; Gomez Lopez, Elda G.
From Phytochemistry (1993), 33(2), 437-45. Language: English, Database: CAPLUS, DOI:10.1016/0031-
9422(93)85535-Y
Cucurbitane-type glycosides were isolated from tuber exts. of Ibervillea sonorae as the major components. Among the
minor constituents, the corresponding aglycons kinoin A (I) and kinoin B (II) and the tetramethylated 19-
norpregnenedione kinoin C (III) have been found. These compds. represent new natural products and the 2,3,6-
trihydroxy-6-methyl-4-heptenyl-2 side chain at C-17 of three of these compds. is unprecedented among the known
curcurbitanes as well as sterols. The structures were detd. by spectroscopic methods in combination with chem.
degrdns. and modifications. Among others, 22-epi-dihydrokinoin A was prepd. The NMR data of the 22-epimers differ
characteristically and allow the establishment of the relative configurations of the vicinal hydroxyl groups at C-20 and C-
22 in this series of compds.

~13 Citings

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15. Some Notes on the Chemical Composition and Toxicity of Ibervillea sonorae
By Emerson, Julia T.; Welker, William H.
From Journal of Biological Chemistry (1909), 5, 339-50. Language: Unavailable, Database: CAPLUS
 SciFinder® Page 6
Besides yielding data for the composition of Ibervillea sonorae the study indicates that the tuber does not contain
alkaloidal material, or at least not in sufficient proportion to cause toxicity. No explanation was found of the cathartic
action of the tuber or its decoctions.
~0 Citings

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16. Ibervillea sonorae (Cucurbitaceae) induces the glucose uptake in human adipocytes by activating a PI3K-
independent pathway
By Zapata-Bustos Rocio; Alonso-Castro Angel Josabad; Gomez-Sanchez Maricela; Salazar-Olivo Luis A
From Journal of ethnopharmacology (2014), 152(3), 546-52, Language: English, Database: MEDLINE
ETHNOPHARMACOLOGICAL RELEVANCE: Ibervillea sonorae (S. Watson) Greene (Cucurbitaceae), a plant used
for the empirical treatment of type 2 diabetes in Mexico, exerts antidiabetic effects on animal models but its mechanism
of action remains unknown. The aim of this study is to investigate the antidiabetic mechanism of an Ibervillea sonorae
aqueous extract (ISE). MATERIALS AND METHODS: Non-toxic ISE concentrations were assayed on the glucose
uptake by insulin-sensitive and insulin-resistant murine and human cultured adipocytes, both in the absence or the
presence of insulin signaling pathway inhibitors, and on murine and human adipogenesis. Chemical composition of
ISE was examined by spectrophotometric and HPLC techniques. RESULTS: ISE stimulated the 2-NBDGlucose
uptake by mature adipocytes in a concentration-dependent manner. ISE 50 µg/ml induced the 2-NBDG uptake in
insulin-sensitive 3T3-F442A, 3T3-L1 and human adipocytes by 100%, 63% and 33%, compared to insulin control.
Inhibitors for the insulin receptor, PI3K, AKT and GLUT4 blocked the 2-NBDG uptake in murine cells, but human
adipocytes were insensitive to the PI3K inhibitor Wortmannin. ISE 50 µg/ml also stimulated the 2-NBDG uptake in
insulin-resistant adipocytes by 117% (3T3-F442A), 83% (3T3-L1) and 48% (human). ISE induced 3T3-F442A
adipogenesis but lacked proadipogenic effects on 3T3-L1 and human preadipocytes. Chemical analyses showed the
presence of phenolics in ISE, mainly an appreciable concentration of gallic acid. CONCLUSION: Ibervillea sonorae
exerts its antidiabetic properties by means of hydrosoluble compounds stimulating the glucose uptake in human
preadipocytes by a PI3K-independent pathway and without proadipogenic effects.

~0 Citings

Copyright © 2017 U.S. National Library of Medicine.

17. Cytotoxic activity of four Mexican medicinal plants

By Vega-Avila Elisa; Espejo-Serna Adolfo; Alarcon-Aguilar Francisco; Velasco-Lezama Rodolfo
From Proceedings of the Western Pharmacology Society (2009), 5278-82, Language: English, Database: MEDLINE

Ibervillea sonorae Greene, Cucurbita ficifolia Bouche, Tagetes lucida Cav and Justicia spicigera Scheltdd are Mexican
native plants used in the treatment of different illnesses. The ethanolic extract of J. spicigera and T. lucida as well as
aqueous extracts from I. sonorae, C. ficifolia, T. lucida and J. spicigera were investigated using sulforhodamine B
assay. These extracts were assessed using two cell line: T47D (Human Breast cancer) and HeLa (Human cervix
cancer). Colchicine was used as the positive control. Data are presented as the dose that inhibited 50% control
growth (ED50). All of the assessed extracts were cytotoxic (ED50 < 20 microg/ml) against T47D cell line, meanwhile
only the aqueous extract from T. lucida and the ethanolic extract from J. spicigera were cytotoxic to HeLa cell line.
Ethanolic extract from J. spicigera presented the best cytotoxic effect. The cytotoxic activity of J. spicigera correlated
with one of the popular uses, the treatment of cancer.

~4 Citings

Copyright © 2017 U.S. National Library of Medicine.

18. Antimicrobial activity of Northwestern Mexican plants against Helicobacter pylori

By Robles-Zepeda Ramon E; Velazquez-Contreras Carlos A; Garibay-Escobar Adriana; Galvez-Ruiz Juan C; Ruiz-
Bustos Eduardo
From Journal of medicinal food (2011), 14(10), 1280-3, Language: English, Database: MEDLINE
 SciFinder® Page 7
Helicobacter pylori is the major etiologic agent of such gastric disorders as chronic active gastritis and gastric
carcinoma. Over the past few years, the appearance of antibiotic-resistant bacteria has led to the development of
better treatments, such as the use of natural products. This study evaluated the anti-H. pylori activity of 17 Mexican
plants used mainly in the northwestern part of Mexico (Sonora) for the empirical treatment of gastrointestinal disorders.
The anti-H. pylori activity of methanolic extracts of the plants was determined by using the broth microdilution method.
The 50% minimum inhibitory concentrations ranged from less than 200 to 400 µg/mL for Castella tortuosa,
Amphipterygium adstringens, Ibervillea sonorae, Pscalium decompositum, Krameria erecta, Selaginella lepidophylla,
Pimpinella anisum, Marrubium vulgare, Ambrosia confertiflora, and Couterea latiflora and were greater than 800 µg/mL
for Byophyllum pinnatum, Tecoma stans linnaeus, Kohleria deppena, Jatropha cuneata, Chenopodium ambrosoides,
and Taxodium macronatum. Only Equisetum gigantum showed no activity against H. pylori. This study suggests the
important role that these plants may have in the treatment of gastrointestinal disorders caused by H. pylori. The
findings set the groundwork for further characterization and elucidation of the active compounds responsible for such
activity.

~4 Citings

Copyright © 2017 U.S. National Library of Medicine.

19. Antibacterial and antifungal activities of some Mexican medicinal plants

By Ruiz-Bustos E; Velazquez C; Garibay-Escobar A; Garcia Z; Plascencia-Jatomea M; Cortez-Rocha M O;
Hernandez-Martinez J; Robles-Zepeda R E
From Journal of medicinal food (2009), 12(6), 1398-402, Language: English, Database: MEDLINE
In Mexico about 4,000 plant species have some medicinal use. The aim of this work was to evaluate the antimicrobial
activity of six Mexican medicinal plants against fungi and Gram-positive and Gram-negative bacteria. Methanolic
extracts were prepared from the Mexican medicinal plants Amphypteringium adstrigens, Castella tortuosa, Coutarea
latiflora, Ibervillea sonorae, Jatropha cuneata, and Selaginella lepidophylla. The antibacterial and antifungal activities
of the plants were determined by the broth microdilution method and the radial growth inhibition assay, respectively.
All Mexican plants tested showed antimicrobial activity. Among the six plant extracts analyzed, J. cuneata showed the
highest growth-inhibitory activity against fungi, Gram-positive and Gram-negative bacteria (J. cuneata > A. adstrigens >
C. latiflora > C. tortuosa > I. sonorae approximately S. lepidophylla). Shigella flexneri and Staphylococcus aureus were
the most susceptible bacteria to plant extracts. Complete inhibition of S. flexneri growth was observed with J. cuneata
methanolic extract at 90 microg/mL. This plant extract also showed the strongest antifungal activity against Fusarium
verticillioides and Aspergillus niger. Our data suggest that the medicinal plants tested have important antimicrobial
properties. This is the first report describing the antimicrobial activities of several of the Mexican medicinal plants used
in this study.

~3 Citings

Copyright © 2017 U.S. National Library of Medicine.

20. Acute and chronic hypoglycemic effect of Ibervillea sonorae root extracts-II
By Alarcon-Aguilar F J; Calzada-Bermejo F; Hernandez-Galicia E; Ruiz-Angeles C; Roman-Ramos R
From Journal of ethnopharmacology (2005), 97(3), 447-52, Language: English, Database: MEDLINE

Ibervillea sonorae's root, or "wareque" (Cucurbitaceae), is widely used in Mexican traditional medicine for the control of
diabetes mellitus. In the present study, the hypoglycemic effects produced by the acute and chronic administration of
various extracts of Ibervillea sonorae were investigated. Both the traditional preparation (aqueous decoction) and the
raw extract (juice) from the root resulted in significant reductions of glycemia in healthy mice after intraperitoneal
administration at a dose of 600 mg/kg. Additionally, ground dried root was used to obtain a dichloromethane (DCM)
extract and a methanol (MeOH) extract. The DCM extract induced a clear reduction of glycemia in healthy (P < 0.05)
and in alloxan-diabetic mice. The intraperitoneally administered DCM extract caused a severe hypoglycemia that
produced lethality in all the treated animals when doses of 300 and 600 mg/kg body weight were used. Since the DCM
extract showed a marked hypoglycemic activity, it was administered daily per os to alloxan diabetic rats, employing
corn oil and tolbutamide as controls. After 41 days of DCM extract administration at a dose of 300 mg/kg/day, diabetic
rats showed improvement in glycemia, body weight, triglycerides, and GPT in comparison with the diabetic control
group. Total cholesterol, GOT, and uric acid blood levels were not affected.

~6 Citings

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