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Gastric juice is the mixture of secretions from all the glands present in the stomach
The total amount of gastric juice secreted per day is about 2 – 3 litres
Gastric juice is highly acidic in nature with a pH of 0.9-1.5.
The gastric juice contains 99.45 % water and 0.55% solid.
Intrinsic Factor :
It is secreted by oxyntic cells of gastric mucous membrane
Intrinsic factor is a glycoprotein
It combines with Vit-B12 (extrinsic factor) and helps in its absorption
Gastric mucin:
Mucin is a glycoprotein
Provides defense against the damage which may be caused by the acid
Gastric mucin is of two types -Visible mucin and soluble mucin
Visible mucin is secreted by surface mucous cells of the gastric mucous membrane
Forms a slippery layer on gastric mucous membrane.
Prevents injury from solid food and moving fluids within the stomach
The soluble mucin secreted by the neck cells
Helps to buffer HCL to some extent
Free acidity: The acidity due to free HCl (not combined with other substances)
present in gastric juice
Total acidity: The acidity due to free HCl, HCl bound to mucin, organic acids, acid
proteins and other acidic substances in gastric juice.
Normal Range:
Free acidity: 18 – 25 mEq/L
Total acidity: 28 – 48 mEq/L
Principle: Free and total acidity of gastric juice is measured by titrating against
know concentration of NaOH using different indicators
Reagents:
Phenolphthalein indicator
Procedure:
Total acidity:
Stop the titration when you observe pale pink color (end point)
Free acidity:
Stop the titration when the colour changes from red to orange-yellow color
Result:
Comment: