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Journal of Strength and Conditioning Research, 2006, 20(1), 167–171

q 2006 National Strength & Conditioning Association

THE EFFECTS OF CARBOHYDRATE LOADING ON


REPETITIVE JUMP SQUAT POWER PERFORMANCE
DISA L. HATFIELD,1 WILLIAM J. KRAEMER,1,2 JEFF S. VOLEK,1 MARTYN R. RUBIN,1
BIANCA GREBIEN,3 ANA L. GÓMEZ,1 DUNCAN N. FRENCH,1 TIMOTHY P. SCHEETT,4
NICHOLAS A. RATAMESS,5 MATTHEW J. SHARMAN,2 MICHAEL R. MCGUIGAN,2
ROBERT U. NEWTON,2 AND KEIJO HÄKKINEN6
1
Human Performance Laboratory, Department of Kinesiology, University of Connecticut, Storrs, Connecticut
06269; 2School of Biomedical and Sports Science, Edith Cowan University, Joondalup, Western Australia,
Australia; 3Department of Movement Sciences, University of Masstricht, Masstricht, Netherlands; 4School of
Human Performance and Recreation, The University of Southern Mississippi, Hattiesburg, Mississippi 39406;
5
Department of Health and Exercise Science, The College of New Jersey, Ewing, New Jersey 08628; 6Department
of Biology of Physical Activity, University of Jyväskylä, P.O. Box 35, 40014 University of Jyväskylä, Finland.

ABSTRACT. Hatfield, D.L., W.J. Kraemer, J.S. Volek, M.R. Rub- crease while in a glycogen-depleted state. Glycogen de-
in, B. Grebien, A.L. Gómez, D.N. French, T.P. Scheett, N.A. Ra- pletion has also been shown to affect high-intensity in-
tamess, M.J. Sharman, M.R. McGuigan, R.U. Newton, and K. termittent sprint performance on a cycle ergometer (1, 27,
Häkkinen. The effects of carbohydrate loading on repetitive 28) and a treadmill (3). Although glycolysis provides the
jump squat power performance. J. Strength Cond. Res. 20(1):
167–171. 2006.—The beneficial role of carbohydrate (CHO) sup-
majority of adenosine triphosphate in exhaustive exercise
plementation in endurance exercise is well documented. How- lasting approximately 3 minutes, CHO ingestion has not
ever, only few data are available on the effects of CHO loading been shown to improve repeated short-duration maximal
on resistance exercise performance. Because of the repetitive use effort exercise lasting less than 5 minutes (10, 14, 16).
of high-threshold motor units, it was hypothesized that the pow- Several studies indicate that glycogenolysis is an im-
er output (power-endurance) of multiple sets of jump squats portant source of energy during resistance training as
would be enhanced following a high-CHO (6.5 g CHO kg body well (5, 7, 14), implying that CHO supplementation may
mass21) diet compared to a moderate-CHO (4.4 g CHO kg body enhance resistance-training results. An acute bout of re-
mass21) diet. Eight healthy men (mean 6 SD: age 26.3 6 2.6
sistance training can significantly decrease muscle gly-
years; weight 73.0 6 6.3 kg; body fat 13.4 6 5.0%; height 178.2
6 6.1 cm) participated in 2 randomly assigned counterbalanced cogen stores (5, 7) and has been shown to result in re-
supplementation periods of 4 days after having their free-living duced isokinetic torque production, decreased isometric
habitual diet monitored. The resistance exercise test consisted strength, and increased fatigue (6, 12). It has been re-
of 4 sets of 12 repetitions of maximal-effort jump squats using ported that mixed-muscle glycogen loss is related to load
a Plyometric Power System unit and a load of 30% of 1 repetition (24), with the rate of glycogenolysis dependent on load
maximum (1RM). A 2-minute rest period was used between sets. and the decrement dependent on the magnitude of muscle
Immediately before and after the exercise test, a blood sample force development (19). Thus, hypotheses related to CHO
was obtained to determine the serum glucose and blood lactate supplementation effects would be dependent on the mag-
concentrations. No significant difference in power performance
existed between the 2 diets. As expected, there was a significant
nitude of glycogen depletion with the exercise protocol.
(p # 0.05) decrease in power performance between the repeti- Carbohydrate supplementation assists in glycogen re-
tions in every set. Blood lactate concentrations were significant- synthesis following both aerobic (2, 20) and anaerobic ex-
ly higher postexercise with both the high-CHO and the moder- ercise bouts and spares glycogen stores (9, 11, 26). Car-
ate- or lower-CHO diet, but there were no differences between bohydrate supplementation has been reported to increase
conditions. The results indicated that the power output during total work output during an acute isokinetic leg exercise
multiple sets of maximal jump squats was not enhanced follow- (8). In addition, CHO supplementation has also been
ing a higher-CHO diet compared to a moderate- or lower-CHO shown to enhance hormonal and hypertrophic responses
diet. These data show that elevated carbohydrate intake is not to chronic resistance training (23).
needed to optimize a repetitive power-endurance performance
when it is done as the first exercise in a workout.
Despite research on intermittent exercise and an
acute bout of resistance training, the effects of differing
KEY WORDS. nutrition, supplementation, power, recovery CHO supplementation on an acute bout of power endur-
ance–type resistance exercise is not known. A typical
high-volume resistance training bout preferentially de-
INTRODUCTION pletes type IIA and IIX fiber (24), although these fibers
utritional strategies are an integral compo- are not well equipped for energy supply and fatigue eas-

N nent of the overall goal of improving athletic


performance. Of these strategies, carbohy-
drate (CHO) supplementation, has repeatedly
been shown to improve endurance perfor-
mance (17, 28). Furthermore, power and time to exhaus-
ily. Power-type exercises, such as squat jumps and bench
press-throws, are used frequently by athletes to increase
power and power-endurance (15). In contrast to a typical
squat exercise, a squat jump exercise utilizes a much
lighter load but takes fuller advantage of the stretch–
tion during repeated short-term bouts of high-intensity shortening cycle to produce higher amounts of explosive
cycling (less than 10 minutes) have been shown to de- power. Explosive power is characterized by faster recruit-
168 HATFIELD, KRAEMER, VOLEK ET AL.

ment of motor units, which may affect rate of glycogen protocol sequence, subjects crossed over to the other se-
resynthesis (18). However, research has clearly demon- quence after a washout period of 2 weeks on their base-
strated that short-term, high-intensity exercise (mea- line diet. Before the study, subjects were educated on how
sured as isokinetic and isometric strength and force pro- to consume the appropriate amount of carbohydrates by
duced) is not affected by diet-induced glycogen depletion a registered dietician. A registered dietician spent time
(22). Thus, it was hypothesized that CHO supplementa- with each subject to screen for diet abnormalities, teach
tion would not have any effect on repetitive power per- subjects how to quantify their food intakes, and educate
formances. The purpose of this study was to directly de- each subject on keeping food records for the study. Food
termine the effects of differing amounts of CHO intakes records were kept and monitoring was done by the nutri-
on an acute bout of power endurance–type resistance ex- tional staff. Food records were analyzed using NUTRI-
ercise protocol typically done at the beginning of a work- TIONIST IV version 3.5.1 software (N-Squared Comput-
out to optimize performance. ing and First DataBank, The Hearst Corporation, San
Bruno, CA) during each baseline and testing segment.
METHODS
Squat Jump Procedures
Approach to the Problem
The squat jump power-endurance exercise protocol con-
Subjects were recruited to participate in 2 randomly as-
sisted of 4 sets of 12 repetitions with maximal effort on
signed counterbalanced supplementation periods of 4
each repetition using the PPS unit with a resistance load
days before commencement of the testing protocol, with
of 30% of 1RM. Maximal mechanical power output was
a washout week between testing segments. The protocol
the goal of the training resistance. Two minutes of rest
consisted of 4 sets of 12 repetitions of maximal jump
was allowed between sets, placing an added metabolic
squats on the Plyometric Power System (PPS) unit (Nor-
stress on the subject. Before the protocol, each subject
search, Lismore, Australia), using a load of 30% of 1 rep-
warmed up by cycling for 5 minutes, performed dynamic
etition maximum (1RM) with 2 minutes of rest between
stretches, and performed 5–10 practice warm-up repeti-
successive sets. The protocol allowed us to see the effects
tions with the squat at 40–60% of his 1RM. With the use
of a demanding repetitive power-endurance-type perfor-
of the on-line computer interfaced with the PPS system,
mance that requires high contribution from glycolysis en-
power output variables were directly assessed.
ergy sources, thereby placing a greater demand on muscle
cell glycogen stores. It was theorized that if the energy Heart Rate Monitoring
demands were significant enough on the type II fast mo-
tor units depleting them of glycogen, power output would Heart rate throughout the protocol was monitored using
be augmented with the additional glycogen concentra- a Polar heart rate monitor (Polart Electro Inc. Medical
tions from a CHO-loading phase. Important to the gen- Division, Lake Success, NY).
eralization of the findings is the fact that the jump squat Biochemical Analyses
protocol was placed at the beginning of a workout.
Blood lactate concentrations were determined using a YSI
Subjects Sport Lactate Analyzer (YSI Inc., Yellow Springs, OH).
Eight healthy men (age 26.3 6 2.6 years; weight 73.0 6 Whole blood was used to determine hemoglobin in dupli-
6.3 kg; body fat 13.4 6 5.0%; height 178.2 6 6.1 cm; 1RM cate using the cyanmethemoglobin method at 540 nm
squat, 125.2 6 16.1 kg) who had only recreational resis- (Sigma Diagnostics, St. Louis, MO), and hematocrit was
tance-training experience of .1 year and were familiar analyzed in triplicate via standard microcapillary tech-
with power training were recruited to participate in this niques and microcentrifugation. Serum glucose concen-
study, which was approved by the Institutional Review trations were measured using a spectrophotometer at 450
Board for use of human subjects. Before the investigation, nm in duplicate with standard colorimetric procedures
the experimental risks, procedures, and benefits were ex- (Sigma Diagnostics, St. Louis, MO). Intra-assay variances
plained to each subject. Each subject then signed an in- were less than 5% for all assays.
stitutionally approved informed consent form if he opted Statistical Analyses
to participate in the study. Subsequently, a medical his-
tory and body composition analysis via skinfolds were ob- Statistical analyses of the data set were accomplished us-
tained during preliminary laboratory visits and familiar- ing an analysis of variance with repeated-measures de-
ization sessions. sign. In the case of significant differences, comparisons
were evaluated with a Fisher’s LSD post-hoc analysis to
Experimental Design and Procedures determine pairwise differences between data distribu-
Free-living habitual diet and physical activity were mon- tions. Analysis was accomplished with Statistica software
itored and recorded for 4 days before testing (baseline (StatSoft, Inc., Tulsa, OK, 1999). From the nQuery Ad-
diet). Each subject was familiarized with the protocol visor software (Statistical Solutions, Saugus, MA), the
testing equipment and their 1RM was determined in the statistical power for the n size used ranged from 0.80 to
squat exercise before testing. The subjects participated in 0.82. The level of significance was set at p # 0.05.
2 randomly assigned counterbalanced supplementation
periods of 4 days before commencement of the testing pro-
RESULTS
tocol, with a washout week between testing segments. A As expected, significant decreases in power were seen af-
crossover design was used with half of the group assigned ter successive repetitions in each set. However, no signif-
to a standard diet with 50% of calories from carbohy- icant differences in peak power were observed. Peak pow-
drates and the other half assigned to a diet with 80% of er output (W) was (mean 6 SD) the following: baseline,
caloric intake from carbohydrates. On completion of 1 set 1, 1,160.1 6 121.8; set 2, 1,154.6 6 127.9; set 3,
CARBOHYDRATES AND JUMP SQUAT PERFORMANCE 169

FIGURE 1. Mean power. *p , 0.05 compared to baseline diet


in same set, and #p $ 0.05 compared to corresponding FIGURE 3. Blood lactate response to the exercise protocol. *p
baseline diet, set 1. # 0.05 from pre-exercise value.

FIGURE 2. Total work for the exercise protocol. *p # 0.05


compared to lower-carbohydrate and higher-carbohydrate
diets.
FIGURE 4. Serum glucose responses to the exercise protocol.
No significant differences in total work were observed between
1,138.8 6 130.7; set 4, 1,110.5 6 131.9; low-CHO diet, set groups or between sets.
1, 1,153.8 6 130.5; set 2, 1,155.8 6 129.5; set 3, 1,165.8
6 112.7; set 4, 1,138.1 6 114.4; high-CHO diet, set 1,
1,183.6 6 92.4; set 2, 1,177.6 6 99.1; set 3, 1,174.0 6 93.7; No significant differences were found in blood glucose
set 4, 1,171.5 6 91.3. concentrations on either the high-carbohydrate or the
Mean power was highest in set 1 for the baseline con- moderate-carbohydrate diet. Mean glucose concentrations
ditions (1,095 6 125 W), highest in set 3 in the 50% CHO in the 50% CHO condition were 5.49 6 0.75 mmol·L21
diet (1,099 6 104), and highest in set 1 in the 80% CHO preprotocol and 5.65 6 0.64 mmol·L21 post. Mean glucose
diet (1,121 6 89). Baseline mean power values were sig- concentrations in the 80% CHO condition were 5.42 6
nificantly lower (p , 0.05) than those of the 80% CHO 0.63 mmol·L21 pre- and 5.83 6 1.03 mmol·L21 postproto-
diet in all 4 sets (set 1, 1,095 6 125 vs. 1,121 6 89 W; set col. These values also did not differ significantly from
2, 1,076 6 131 vs. 1,108 6 88 W; set 3, 1,049 6 128 vs. baseline or washout values (Figure 3).
1,086 6 76 W; set 4, 1,037 6 119 vs. 1,086 6 72 W), and Mean lactate concentrations for the 50% CHO group
lower than those of the 50% CHO diet in sets 3 and 4 significantly increased from baseline values in both
(1,099 6 104 and 1,094 6 98, respectively.) The baseline groups (from 1.15 6 0.39 mmol·L21 pre to 9.56 6 3.34
diet condition exhibited significant decreases in mean mmol·L21 post in 50% CHO and 1.46 6 0.33 mmol·L21 pre
power in sets 3 and 4 compared to set 1, but these same to 9.32 6 3.37 mmol·L21 post in 80% CHO). However, this
decreases were not apparent in the other 2 diet condi- increase did not differ significantly between the 50%
tions. CHO and 80% CHO diets (Figure 4).
Also of interest is the significant relative decrease in There were few differences in the type of CHOs in-
mean power in the baseline diet condition (a 5% de- gested among the 3 diets. Subjects consumed an average
crease). The relative difference from set 1 to set 4 was not of 125.6 6 47.8 g of sugar CHOs per day on the 50% CHO
significantly different in the 50% CHO and 80% CHO con- diet, which was significantly different from the 186.7 6
ditions. However, this 5% decrease was significantly dif- 27.9 g they consumed per day on the 80% CHO diet. Sig-
ferent from the 0.05% change in mean power in the 50% nificant differences in the amount of dietary fiber con-
CHO diet condition. sumed also existed between the 50% CHO and 80% CHO
The only significant differences in total work per set diets (17.6 6 5.0 g vs. 25.8 6 6.8 g). Also, when expressed
were observed among the 3 diet conditions in the second as a percentage of daily calories, subjects consumed sig-
set. Total work was highest in the baseline diet (9.43 6 nificantly more sugar on the 80% CHO diet compared to
0.92 J) compared to the 50% CHO (8.90 6 1.03 J) and the 50% CHO diet (31.8 6 4.1% vs. 22.3 6 6.5%) and more
80% CHO groups (8.98 6 1.03 J). Total work among the fiber on the 80% CHO diet than on baseline and 50%
4 sets did not differ for any of the diets (see Figures 1 CHO diets (80% CHO, 4.4% 6 0.7%; baseline, 3.5% 6
and 2). 0.7%; 50% CHO, 3.3% 6 1.0%).
170 HATFIELD, KRAEMER, VOLEK ET AL.

DISCUSSION eccentric component and recruitment pattern of the mus-


cle fiber differ from those in heavier loads that are moved
The primary finding of this study was that an 80% daily at a slower rate. This load was chosen in order to train
caloric intake from carbohydrates has no effect on repet- at the maximal mechanical power output because such
itive power output (power-endurance) in an acute train- repetitive power output is often used in sports such as
ing session compared to a 50% CHO diet. However, it did wrestling, track, basketball, and rugby. The demands of
significantly enhance mean maximum power output such exercise is great to maintain the quality of the high
when compared to a self-selected (baseline) diet. With the levels of power produced with such a load, making the
80% CHO diet, mean maximum power output was higher exercise challenge for the motor units mediating the ex-
than the baseline diet of 55% CHO. However, there were ercise quite extreme.
no differences for this variable when compared to the con- This study focused solely on an acute bout of power-
trolled 50% CHO diet. Explanations remain speculative endurance resistance exercise. There are many potential
but may implicate the diet compositions (controlled and benefits of CHO supplementation on a chronic basis in
instructed 50% CHO vs. self-selected 55% CHO), as reli- conjunction with resistance training. Carbohydrate sup-
ability of the test protocol was very high. Without biopsy plementation improves the anabolic environment by in-
samples, it was not clear that the exercise protocol was creasing plasma insulin concentrations, promoting pro-
capable of actually depleting muscle glycogen stores fully. tein synthesis, and decreasing the rate of protein degra-
The 2-minute rest period between sets may have been dation (20, 21, 25). However, further studies are needed
sufficient to help restore energy levels enough to main- to quantify this relationship as it pertains to power-type
tain power outputs, albeit obviously reduced with contin- exercises by varying total volume of exercise, rest be-
ued subsequent sets. In one investigation, neither type tween sets, and muscle group involved.
IIA muscle fibers not type IIX 1 IIA muscle fibers were Dietary choices may have also played a role in the
totally depleted of muscle glycogen stores in response to results. The mean CHO content of the baseline diet was
an acute bout of leg extensions at 30, 45, and 60% of 1RM 55%, so it seems contradictory that mean power would
(24). Although this study utilized a 30% load, the number differ significantly from the 80% CHO group although the
of motor units and the subsequent recruitment of muscle 50% CHO group did not. However, there was an extreme-
fibers may be different because of the whole-body struc- ly large range of CHO intake between the subjects for the
tural nature of the ballistic exercise used in this study. baseline diets (from 49 to 62%). This variance may be
With regard to total work, it is possible that more than 4 enough to account for the significant difference. Also,
sets would be required to dramatically stress metabolic there was a large variance in fat and protein intake
energy stores. The exercise session was also placed at the among all 3 diets. Although the type of CHO does not
beginning of the workout with all muscle fibers at their appear to affect peak power, it may be possible that the
greatest energy storage potential. The placement of the subjects’ food choices affected differences seen in mean
protocol is typical of most power-oriented training ses- power between the 80% CHO and baseline diets. The sub-
sions in that one is trying to produce optimum levels of jects consumed significantly more fiber as a percentage
power output from the rested condition. However, the re- of total calories on the 80% CHO diet compared to the
sults might have differed dramatically if the squat jumps baseline diet. However, future studies are needed to in-
had been performed at the end of a workout that stressed vestigate the effects of substrate ratio on repetitive power
the leg musculature or other high-intensity conditioning output.
exercises. Thus, such order effects require further study.
However, the eccentric component of the exercise in PRACTICAL APPLICATIONS
this study may have led to greater glycogen depletion
than previously reported in other studies in which con- High-intensity resistance training does deplete muscle
centric-only actions were used. It has been reported that glycogen, and that rate of muscle glycogen resynthesis is
fast-twitch fibers, such as those used in short-term high- increased with CHO supplementation (7). However, in an
intensity exercise, may have a higher rate of muscle gly- acute bout of short-duration maximal exercise, it appears
cogen resynthesis because of their high glycogen synthase that a high-CHO (80%) diet has no effect on power-en-
activity and depletion rate during high-intensity exercise durance performance compared to a moderate-CHO (50%)
(13). Had muscle glycogen stores been fully depleted, this diet. Placement of the protocol in a resistance exercise
workout may be another important consideration, as our
phenomenon might have taken place. Again, the place-
data can be generalized only when the squat jump pro-
ment of the squat jumps in a workout session may be of
tocol is placed at the beginning of a resistance workout
importance to such effects. Also, this study only focused
routine.
on blood glucose concentrations, as opposed to muscle gly-
cogen concentrations. Because glycogen resynthesis rates
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