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MEMBRANE

SELECTION
GUIDE
Membranes for nucleic acid blotting 4

Hybond Atlas Arrays 8

Hybond Northern Blots 9

Membranes for protein blotting 10

Hybond Blotting Paper 12

Related products 13

Hybond
Amersham Biosciences is a world leader in membrane-based labelling,
detection, and analysis. Hybond™ membranes combined with specialized
reagents, instruments, and software provide the complete solutions for all
membrane-based applications.
We provide the largest selection of membranes available for life science
research, including our most popular formulations: Hybond-N+ and Hybond
ECL™ and have also recently added two exciting new ranges: Hybond
Atlas™ cDNA Expression Arrays and Hybond Northern Blots.

1
IN YOUR RESULTS
All Hybond membranes manufactured by Amersham Biosciences are manufactured
specifically for life science applications.

• Stringent quality control and functional testing ensure consistent performance.


• Production runs are carefully controlled and the end-product exhaustively screened to
make sure that only the most consistent product reaches you.
• Each type of membrane is designed to give the best and most reliable results in
each application.
• For larger orders, lot testing, and our batch reservation service enable you to validate
specific membrane for your needs.
• For convenience, membrane can be customized to meet your specific needs:
dimension and pack size.
• Superior service and technical support: Individual technical advice is available on all
aspects of blotting and hybridization via the Hybond Helpline, staffed with
knowledgeable scientists.

Contact us at hybond_help@uk.amershambiosciences.com

2
OF HYBOND MEMBRANES

There are two distinct types of membrane:


• Unsupported, where the active substrate is present as a cast sheet (e.g. Hybond ECL
nitrocellulose membrane).
• Supported, where the active substrate is present and cast on to an inert 'web' or support
(e.g. Hybond-N [nylon], Hybond-N+ [charged nylon], and Hybond-C Extra [nitrocellulose]).

Membranes for nucleic acid blotting Membranes for protein blotting

Product
name Hybond-N+ Hybond-XL Hybond-N Hybond-P Hybond ECL Hybond-C Extra

Physical Strong web Strong web Strong web High compared Fragile, especially Strong web
strength structure structure structure to unsupported after baking structure
nitrocellulose

Molecules ssDNA ssDNA ssDNA Proteins Proteins Proteins


bound dsDNA dsDNA dsDNA
most RNA RNA RNA
efficiently

Binding 600 µg/cm2 600 µg/cm2 600 µg/cm2 125 µg/cm 2 100 µg/cm 2 80-100 µg/cm 2
capacity

Fixing of Alkali fixation- UV cross-linking UV cross-linking


nucleic rapid, UV recommended, recommended
acid blots cross-linking- baking possible,
best for no vacuum
maximum required
reproducibility,
baking possible-
no vacuum
required

Nucleic Excellent for Designed for Ideal for


acid radioactive or radioactive radioactive
detection non radioactive detection detection
and detection and
reprobing high-density
arrays

Protein Excellent for Excellent for Excellent for


detection ECL plus, ECF ECL or radioactive
or chromogenic chromogenic detection
detection

Reprobing Excellent Not Recommended


protein recommended

Electro- DNA and RNA DNA and RNA DNA and RNA Proteins Proteins Proteins
blotting (recommended
for expression
screening)

All Amersham Biosciences Hybond membranes are identical on each side. Therefore it
does not matter which side you use.

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HYBOND MEMBRANES FOR
Hybond membranes are available to support all major nucleic acid blotting applications.
The following table is designed to help in the choice of the best possible membrane for
each application.

Application Method Hybond-N+ Hybond-XL Hybond-N


Southern Radioactive Highly Highly Recommended
blotting detection recommended recommended
Non-radioactive Highly Not Not
detection recommended recommended recommended
(ECL/AlkPhos
DirectTM/Gene
ImagesTM)
Alkali blotting/ Highly Highly Unsuitable
fixation recommended recommended
Rapid-hyb™ Highly Highly Suitable
Buffer recommended recommended
Low volume Suitable Highly Unsuitable
hybridization recommended
High density Highly Not Recommended
arrays recommended recommended
Northern Radioactive Recommended Highly Recommended
blotting detection recommended
Non-radioactive Highly Not Not
detection recommended recommended recommended
DNA dot/ Radioactive Recommended Highly Recommended
slot detection recommended
Non-radioactive Recommended Not Not
detection recommended recommended
Colony/plaque Radioactive Suitable Recommended Recommended
detection
Non-radioactive Recommended Not Suitable
detection recommended

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Hybond N+
Hybond-N+ is a positively charged nylon membrane that yields excellent sensitivity in all
nucleic acid applications. Nucleic acid samples may be fixed by simple alkali treatment or
alkali blotting rather than UV exposure or baking; however, UV fixation is recommended
for maximum reproducibility. Hybond N+ is recommended for both radioactive and non-
radioactive protocols.
Hybond-N+ also has excellent properties for high-density macroarrays.
• Excellent binding capacity
• Highest sensitivity
• Discrete, fixed, and uniform colony growth across the membrane
• Uniform and rapid wetting
• Mechanically stable and flat
• Uniform, defect-free surface

Ordering information
Product Quantity Code

Hybond-N+ (82 mm) 50 discs RPN82B


Hybond-N+ (87 mm) 50 discs RPN87B

Hybond-N+ (132 mm) 50 discs RPN132B

Hybond-N+ (137 mm) 50 discs RPN137B


Hybond-N+ (11.9 × 7.8 cm) 50 sheets RPN119B

Hybond-N+ (11.5 × 7.3 cm) 50 sheets RPN1576B+


Hybond-N+ (22.2 × 22.2 cm) 50 sheets RPN2250B*

Hybond-N+ (22.5 × 22.5 cm) 50 sheets RPN225B *

Hybond-N+ (12 × 10 cm) 20 sheets RPN1210B


Hybond-N+ (15 × 10 cm) 20 sheets RPN1510B

Hybond-N+ (15 × 20 cm) 10 sheets RPN1520B

Hybond-N+ (20 × 20 cm) 10 sheets RPN2020B


Hybond-N+ (22.2 × 22.2 cm) 10 sheets RPN2222B

Hybond-N+ (30 × 50 cm) 5 sheets RPN3050B

Hybond-N+ (20 cm × 3 m) 1 roll RPN203B


Hybond-N+ (30 cm × 3 m) 1 roll RPN303B

Hybond-N+ (82 mm) 50 gridded discs RPN1782B

Hybond-N+ (87 mm) 50 gridded discs RPN1787B


Hybond-N+ (132 mm) 50 gridded discs RPN1732B

Hybond-N+ (137 mm) 50 gridded discs RPN 1737B


+
Designed to fit the OmniTray from Nalgen Nunc International * Especially for high-density membrane arrays
5
Hybond-XL
Hybond-XL is a charged nylon membrane designed exclusively for radioactive nucleic
acid transfer applications to achieve an improved signal-to-noise ratio. It has a greater
concentration and a more even distribution of positive charge than other charged nylon
membranes. Hybond-XL produces excellent results with a wide variety of applications
and target/probe combinations. This membrane retains all the advantages of other nylon
membranes such as high nucleic acid binding capacity and high tensile strength.

Ordering information
Product Quantity Code

Hybond-XL (82 mm) 50 discs RPN82S

Hybond-XL (87 mm) 50 discs RPN87S

Hybond-XL (132 mm) 50 discs RPN132S


Hybond-XL (137 mm) 50 discs RPN137S

Hybond-XL (11.9 × 7.8 cm) 50 sheets RPN119S

Hybond-XL (12 × 10 cm) 20 sheets RPN1210S


Hybond-XL (15 × 10 cm) 20 sheets RPN1510S

Hybond-XL (15 × 20 cm) 10 sheets RPN1520S

Hybond-XL (20 × 20 cm) 10 sheets RPN2020S


Hybond-XL (22.2 × 22.2 cm) 10 sheets RPN2222S

Hybond-XL (30 × 50 cm) 5 sheets RPN3050S

Hybond-XL (20 cm × 3 m) 1 roll RPN203S

Hybond-XL (30 cm × 3 m) 1 roll RPN303S

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Hybond-N
Hybond-N is a neutral nylon membrane capable of high sensitivity in DNA and RNA
blotting. It is a strong, supported membrane that is inherently hydrophilic and requires
no pre-wetting. It is easy to quickly cross-link nucleic acids to nylon using UV light. This
makes Hybond-N ideal for any standard radioactive Southern or Northern blotting
procedure.

Ordering information
Product Quantity Code

Hybond-N (82 mm) 50 discs RPN82N

Hybond-N (87 mm) 50 discs RPN87N

Hybond-N (132 mm) 50 discs RPN132N


Hybond-N (137 mm) 50 discs RPN137N

Hybond-N (11.9 × 7.8 cm) 50 sheets RPN119N

Hybond-N (12 × 10 cm) 20 sheets RPN1210N


Hybond-N (15 × 10 cm) 20 sheets RPN1510N

Hybond-N (15 × 20 cm) 10 sheets RPN1520N

Hybond-N (20 × 20 cm) 10 sheets RPN2020N


Hybond-N (22.2 × 22.2 cm) 10 sheets RPN2222N

Hybond-N (30 × 50 cm) 5 sheets RPN3050N

Hybond-N (20cm × 3m) 1 roll RPN203N

Hybond-N (30cm × 3m) 1 roll RPN303N

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PRODUCT RANGES
Hybond Atlas cDNA Expression Arrays
Hybond Atlas Array products represent a comprehensive range of high-density pre-
arrayed membranes that enable the expression of significant numbers of genes to be
screened simultaneously. These macroarrays each contain hundreds of carefully selected,
well characterized, arrayed cDNAs and offer the highest quality expression data.
Combining Amersham Biosciences' expertise in membrane hybridization and detection
with Clontech's expertise in expression arrays, Hybond Atlas Arrays are carefully
designed, pre-made macroarrays produced specifically for Amersham Biosciences by
Clontech Laboratories Inc.
Hybond Atlas Arrays provide sophisticated information, but are relatively inexpensive and
simple to use. They are designed to be accessible to all laboratories performing isotopic
blot hybridization experiments and optimized to be screened using RedivueTM nucleotides.
They are visualized using standard procedures, such as scanning with our range of
Typhoon™ Variable Mode Imagers, and are supplied with labelling, purification, and
hybridization reagents.
Hybond Atlas RNA Purification and Labelling Kit combines the purification of total
RNA and subsequent labelling of cDNA probes to be used with Hybond Atlas Arrays
in one experiment.
Ordering information
Product Quantity Code

Hybond Atlas Array Mouse 1.2 1 kit RPN7853


Hybond Atlas Array Rat 1.2 1 kit RPN7854
Hybond Atlas Array Human 1.2 1 kit RPN7850
Hybond Atlas Array Human Cancer 1.2 1 kit RPN7851
Hybond Atlas Array Human 1.2 II 1 kit RPN7852
Hybond Atlas Array Human 1.2 III 1 kit RPN7855
Hybond Atlas Array Mouse 1.2 II 1 kit RPN7857
Hybond Atlas Array Rat 1.2 II 1 kit RPN7856
Hybond Atlas Array Mouse Cancer 1.2 1 kit RPN7858
Hybond Atlas Array Human Trial Kit 1 kit RPN7882
Hybond Atlas RNA Purification 1 kit 25-800-567
and Labelling Kit

Full details of all these products can be found at:

www.amershambiosciences.com/hybondatlas
or by contacting your local office.

8
Hybond Northern Blots
Hybond Northern Blots are ready-made, ready to hybridize RNA blots for gene function studies:
• Analysis of the size and relative abundance of transcripts in different tissues
• Investigation of gene families and alternative splice forms
• Assessment of cross-species homology using different Hybond Northern Blots
There are five types of Hybond Northern Blot, each of which includes the highest
quality Poly A+ RNA from a variety of tissues or cell lines from a single species, either
human or mouse.

Lane Human a Human b Human c Human d Mouse a


Number 8 Poly A+ RNA 7 Poly A+ RNA 8 Poly A+ RNA 12 Poly A+ RNA 8 Poly A+ RNA
RPN4801 RPN4802 RPN4803 RPN4800 RPN4804
1 Heart Stomach Spleen Brain Heart
2 Brain Thyroid Thymus Heart Brain
3 Placenta Spinal cord Prostate Skeletal muscle Spleen
4 Lung Lymph node Testis Colon Lung
5 Liver Trachea Uterus Thymus Liver
6 Skeletal muscle Adrenal gland Small intestine Spleen Skeletal muscle
7 Kidney Bone marrow Colon Kidney Kidney
8 Pancreas Peripheral blood Liver Testis
leukocyte
9 Small intestine
10 Placenta
11 Lung
12 Peripheral blood
leukocyte

Tissue representation (as they appear on each Northern blot)

Ordering information
Product Quantity Code
Human a Northern Blot 1 kit RPN4801
Human b Northern Blot 1 kit RPN4802

Human c Northern Blot 1 kit RPN4803

Human d Northern Blot 1 kit RPN4800


Mouse a Northern Blot 1 kit RPN4804

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HYBOND MEMBRANES FOR
A choice of three different Hybond formulations for Western blotting means we have the
right membrane for your application.

Application/ Hybond-P Hybond ECL Hybond-C Extra


detection method
ECL Highly recommended Highly recommended Suitable
ECL Plus Highly recommended Recommended Suitable

Chromogenic Recommended Highly recommended Suitable

Colloidal Gold Recommended Highly recommended Not recommended


ECF Highly recommended Suitable Not recommended

Radioactive Suitable Suitable Highly recommended

Glycoprotein Highly recommended Recommended Suitable


Reprobing Westerns Highly recommended Not recommended Suitable
Expression screening Suitable Not recommended Highly recommended

Hybond-P
A hydrophobic polyvinylidene difluoride (PVDF) membrane optimized for use in protein
transfers. With higher physical strength than unsupported nitrocellulose, Hybond-P offers
significant handling advantages and is ideal for reprobing. It is particularly recommended
for use with ECL Plus™ Western Blotting Detection Reagents and gives excellent results
with ECL Glycoprotein Detection System.

• High mechanical strength with a protein binding capacity of 125 µg/cm2.


• Chemically stable, allowing the use of a range of solvents for rapid destaining.

Ordering information
Product Quantity Code

Hybond-P (20 × 20 cm) 10 sheets RPN2020F


Hybond-P (30 cm × 3 m) 1 roll RPN303F

Hybond-P (14 x 16 cm) 15 sheets RPN1416F

10
Hybond ECL
An unsupported 100% nitrocellulose membrane that delivers excellent sensitivity,
resolution, and low background for all labelling and detection systems.

• Validated for use with the ECL Western Blotting System.


• Special packaging features enhance membrane performance in terms
of handling and consistency of results. Includes interleaf on both sides
of the membrane and air-tight, re-sealable aluminum bags with label
space for marking the "opened on" date.

Ordering information
Product Quantity Code
Hybond ECL (82 mm) 50 discs RPN82D

Hybond ECL (132 mm) 50 discs RPN132D


Hybond ECL (137mm) 50 discs RPN137D

Hybond ECL (6 × 8 cm) 50 sheets RPN68D

Hybond ECL (7 × 8 cm) 50 sheets RPN78D


Hybond ECL (9 × 10.5 cm) 10 sheets RPN910D

Hybond ECL (10 × 10 cm) 10 sheets RPN1010

Hybond ECL (15 × 15 cm) 10 sheets RPN1515D

Hybond ECL (15 × 20 cm) 10 sheets RPN1520D


Hybond ECL (20 × 20 cm) 10 sheets RPN2020D

Hybond ECL (20 cm × 3 m) 1 roll RPN203D

Hybond ECL (30 cm × 3 m) 1 roll RPN3032D*


Hybond ECL (30 cm × 3 m) 1 roll RPN303D

*0.2 mm pore size

11
Hybond-C Extra
A supported mixed ester nitrocellulose with high physical strength. Hybond-C Extra
may be used in all protein transfer and detection procedures, but is particularly
recommended for expression screening because it gives high signal-to-noise and is easy
to handle.

• Supporting web allows multiple reprobing without loss of membrane integrity

Ordering information
Product Quantity Code
Hybond-C Extra (82 mm) 50 discs RPN82E
Hybond-C Extra (137 mm) 50 discs RPN137E
Hybond-C Extra (20 × 20 cm) 10 sheets RPN2020E
Hybond-C Extra (20 cm × 3 m) 1 roll RPN203E
Hybond-C Extra (30 cm × 3 m) 1 roll RPN303E

Hybond Blotting Paper


• Research laboratory blotting paper for use in gel electrophoresis in all molecular
biology and protein studies applications
• Recommended for use with all Hybond membranes
• High purity and uniformity ensures consistent and reproducible results
• Pure cellulose grade, 0.39 mm-thick paper with medium wet-strength and
smooth surface

Ordering information
Product Quantity Code
Hybond Blotting Paper (46 × 57 cm) 100 sheets RPN6100M
Hybond Blotting Paper (20 × 20 cm) 100 sheets RPN6101M
Hybond Blotting Paper (23 cm × 100 m) 1 roll RPN6102M
Hybond Blotting Paper (19 cm × 100 m) 1 roll RPN6103M
Hybond Blotting Paper (27 cm × 100 m) 1 roll RPN6104M

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FOR NUCLEIC ACID ANALYSIS
Nucleic acid purification
QuickPrepTM Total RNA Extraction Kit 27-9271-01
QuickPrep Micro mRNA Purification Kit 27-9254-01
GFX™ Genomic Blood DNA Purification Kit 27-9603-01

GFX PCR DNA and Gel Band Purification Kit 27-9602-01


FlexiPrep™ Kit 27-9281-01

MicroSpin™ G-50 Columns 27-5330-01

Labelling kits
RediprimeTM II DNA Labelling System RPN1634
Megaprime™ DNA Labelling System RPN1604
HotScribe™ First-Strand cDNA Labelling Kit RPN5650
AlkPhos Direct Labelling and Detection System RPN3690
ECL Direct™ Labelling and Detection System RPN3000
Gene Images Random Prime Labelling and Detection System RPN3500
ECFTM Random Prime Labelling and Detection System RPN5752
Ready-To-GoTM You Prime First-Strand Beads 27-9264-01
First-Strand cDNA Synthesis Kit 27-9261-01

Radioactively labelled nucleotides


Full range of 32P/33P radioactively labelled nucleotides including Redivue formulations

Products for Microarray labelling


CyDyeTM-labelled nucleotides
CyTM3-dCTP PA53021
Cy5-dCTP PA55021
Cy3-dUTP PA53022
Cy5-dUTP PA55022
CyScribe™ First-Strand cDNA Labelling Module 25 reactions RPN6200
CyScribe First-Strand cDNA Labelling Kit 50 reactions RPN6201
with 25 nmol Cy3-dUTP and 25 nmol Cy5-dUTP
CyScribe First-Strand cDNA Labelling Kit 50 reactions RPN6202
with 25 nmol Cy3-dCTP and 25 nmol Cy5-dCTP
CyScribe Post-Labelling Kit RPN5660
CyDye Post-Labelling Reactive Dye Pack RPN5661
CyScribe Direct mRNA Labelling Kit RPN5665

13
PCR amplification
Taq DNA Polymerase RPN0303Z
Ready-To-Go PCR Beads 27-9553-02
PCR Nucleotide Mix US77119

Additional products
Rapid-hyb™ Buffer RPN1636
Ultrapure biochemicals
Restriction and modifying enzymes
Ultrapure solution dNTPs

14
FOR PROTEIN ANALYSIS
Blocking agents
ECL Blocking Agent RPN2125
Bovine Serum Albumin RPN412
Fish Gelatin RPN416
Liquid Blocking Reagent RPN3601

Secondary antibodies
Peroxidase-linked
Mouse IgG, HRP-linked Whole Ab from Sheep NA931
Mouse IgG, HRP-linked F(ab')2 Fragment from Sheep NA9310
Human IgG, HRP-linked Whole Ab from Sheep NA933
Rabbit IgG, HRP-linked Whole Ab from Donkey NA934
Rabbit IgG, HRP-linked F(ab')2 Fragment from Donkey NA9340
Rat IgG, HRP-linked Whole Ab from Goat NA935
Rat IgG, HRP-linked F(ab')2 Fragment from Goat NA9350
Anti GST, HRP-linked Polyclonal Ab from Goat RPN1236

Biotinylated antibodies
Mouse IgG, Whole Ab from Sheep, biotinylated RPN1001
Human IgG, Whole Ab from Sheep, biotinylated RPN1003
Rabbit IgG, Whole Ab from Donkey, biotinylated RPN1004
Rat IgG, Whole Ab from Goat, biotinylated RPN1005

Fluor-linked antibodies
CyDye Conjugates
Mouse IgG, Whole Ab from Sheep, Fluorescein-linked N1031
Rabbit IgG, Whole Ab from Donkey, Fluorescein-linked N1034
Mouse IgG, Whole Ab from Sheep, Texas Red-linked N2031
Rabbit IgG, Whole Ab from Donkey, Texas Red-linked N2034

Streptavidin-Enzyme conjugates
Streptavidin-Biotinylated HRP Complex RPN1051
Streptavidin-HRP Conjugate RPN1231
ECL Streptavidin-HRP Conjugate (500 ml) and Blocking Reagent (44 g) RPN2195

15
Detection reagents
ECL Western Blotting System RPN2108
ECL Western Blotting Reagents for 1000 cm2 membrane RPN2109
ECL Western Blotting Reagents for 2000 cm2 membrane RPN2209
ECL Western Blotting Reagents for 4000 cm membrane
2
RPN2106
ECL Plus Western Blotting Reagents for 1000 cm2 membrane RPN2132
ECL Plus Western Blotting Reagents for 3000 cm2 membrane RPN2133
ECL Plus Western Blotting Reagent Pack * RPN2124
*Does not contain detection reagents

ECL kits
ECL Glycoprotein Detection Module* RPN2190
ECL Protein Biotinylation System RPN2203
ECL Protein Biotinylation Module* RPN2202
ECL Phosphorylation Module* RPN2220
ECL GST Western Blotting Detection Kit RPN1237
*Order ECL Detection Reagents separately

Hyperfilm ECL - New formulation film up to 30% more sensitive


RPN2103K (18 × 24 cm) 25 Sheets
RPN3103K (18 × 24 cm) 75 Sheets
RPN2104K (30 × 40 cm) 25 Sheets
RPN1674K (5 × 7 in) 25 Sheets
RPN2114K (8 × 10 in) 25 Sheets
RPN3114K (8 × 10 in) 75 Sheets
RPN1681K (10 × 12 in) 25 Sheets

Scanning instrumentation/analysis software


Molecular Dynamics scanner series:
Typhoon™ Variable Mode Imager
Storm™
Phosphorimager™ SI
Fluorlmager™ 595
ArrayVision™ Software

16
for further details contact your local office
Asia Pacific Tel: +852 2811 8693 Fax: +852 2811 5251 Australasia Tel: + 61 2 9899 0999 Fax: +61 2 9899 7511
Austria Tel: 01 576 0616 25 Fax: 01 576 0616 27 Belgium Tel: 0800 73 888 Fax: 03 272 1637 Canada Tel: 1 800 463 5800 Fax: 1 800 567 1008
Central, East, and South East Europe Tel: +43 1 982 3826 Fax: +43 1 985 8327 Denmark Tel: 45 16 2400 Fax: 45 16 2424
Finland & Baltics Tel: +358-(0)9-512 39 40 Fax: +358-(0)9-512 17 10 France Tel: 01 6935 6700 Fax: 01 6941 9677
Germany Tel: 0761 4903 106 Fax: 0761 4903 405 Italy Tel: 02 27322 1 Fax: 02 27302 212 Japan Tel: +81 3 5331 9336 Fax: +81 3 5331 9370
Latin America Tel: +55 11 3667 5700 Fax: +55 11 3667 87 99 Middle East and Africa Tel: +30 (1) 96 00 687 Fax: +30 (1) 96 00 693
Netherlands Tel: 0165 580 410 Fax: 0165 580 401 Norway Tel: 2318 5800 Fax: 2318 6800 Portugal Tel: 21 417 7035 Fax: 21 417 3184
Russia & other C.I.S. & N.I.S Tel: +7 (095) 232 0250, 956 1137 Fax: +7 (095) 230 6377 South East Asia Tel: 60 3 8024 2080
Fax: 60 3 8024 2090 Spain Tel: 93 594 49 50 Fax: 93 594 49 55 Sweden Tel: 018 612 1900 Fax: 018 612 1910 Switzerland Tel: 01 802 81 50
Fax: 01 802 81 51 UK Tel: 0800 616928 Fax: 0800 616927 USA Tel: +1 800 526 3593 Fax: +1 877 295 8102

or visit us at:

www. amershambiosciences.com

CyDye or portion thereof is manufactured under licence from Carnegie


Mellon University, US Patent Numbers 5,268,486 and 5,569,587 and
Patent Application number WO 99/31181. CyScribe, CyScribe Direct,
ECF, FlexiPrep, FluorImager, Gene Images, GFX, HotScribe, Hybond,
AlkPhos Direct, Cy, CyDye, Megaprime, MicroSpin, PhosphorImager,
QuickPrep, Rapid-hyb, Ready-To-Go, Rediprime, Redivue, Storm and
Typhoon are trademarks of Amersham Biosciences Limited or its
subsidiaries. Drop Design is a trademark of Amersham Biosciences
Limited. Amersham and Amersham Biosciences are trademarks of
Amersham plc. ArrayVision is a trademark of Imaging Research Inc.
Atlas is a trademark of Clontech Laboratories Inc.
© Amersham Biosciences UK 2002 - All rights reserved. All goods and
services are sold subject to the terms and conditions of sale of the
company within the Amersham Biosciences group which supply them.
A copy of these terms and conditions are available on request. Some of
these products may only be available to collaborators and customers
within certain of our technology access programmes.

Amersham Biosciences Amersham Place Little Chalfont, Buckinghamshire


England U.K. HP7 9NA.
Amersham Biosciences AB SE-751 84 Uppsala Sweden.
Amersham Biosciences Corp 800 Centennial Avenue PO Box 1327
Piscataway NJ 08855 USA.
Amersham Biosciences EuropeGmbH Munzinger Strasse 9 D-79111
Freiburg Germany.

*Atlas is a trademark of Clontech Inc.

18-1161-28AA

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