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Membranes for nucleic acid blotting 4
Related products 13
Hybond
Amersham Biosciences is a world leader in membrane-based labelling,
detection, and analysis. Hybond™ membranes combined with specialized
reagents, instruments, and software provide the complete solutions for all
membrane-based applications.
We provide the largest selection of membranes available for life science
research, including our most popular formulations: Hybond-N+ and Hybond
ECL™ and have also recently added two exciting new ranges: Hybond
Atlas™ cDNA Expression Arrays and Hybond Northern Blots.
1
IN YOUR RESULTS
All Hybond membranes manufactured by Amersham Biosciences are manufactured
specifically for life science applications.
Contact us at hybond_help@uk.amershambiosciences.com
2
OF HYBOND MEMBRANES
Product
name Hybond-N+ Hybond-XL Hybond-N Hybond-P Hybond ECL Hybond-C Extra
Physical Strong web Strong web Strong web High compared Fragile, especially Strong web
strength structure structure structure to unsupported after baking structure
nitrocellulose
Binding 600 µg/cm2 600 µg/cm2 600 µg/cm2 125 µg/cm 2 100 µg/cm 2 80-100 µg/cm 2
capacity
Electro- DNA and RNA DNA and RNA DNA and RNA Proteins Proteins Proteins
blotting (recommended
for expression
screening)
All Amersham Biosciences Hybond membranes are identical on each side. Therefore it
does not matter which side you use.
3
HYBOND MEMBRANES FOR
Hybond membranes are available to support all major nucleic acid blotting applications.
The following table is designed to help in the choice of the best possible membrane for
each application.
4
Hybond N+
Hybond-N+ is a positively charged nylon membrane that yields excellent sensitivity in all
nucleic acid applications. Nucleic acid samples may be fixed by simple alkali treatment or
alkali blotting rather than UV exposure or baking; however, UV fixation is recommended
for maximum reproducibility. Hybond N+ is recommended for both radioactive and non-
radioactive protocols.
Hybond-N+ also has excellent properties for high-density macroarrays.
• Excellent binding capacity
• Highest sensitivity
• Discrete, fixed, and uniform colony growth across the membrane
• Uniform and rapid wetting
• Mechanically stable and flat
• Uniform, defect-free surface
Ordering information
Product Quantity Code
Ordering information
Product Quantity Code
6
Hybond-N
Hybond-N is a neutral nylon membrane capable of high sensitivity in DNA and RNA
blotting. It is a strong, supported membrane that is inherently hydrophilic and requires
no pre-wetting. It is easy to quickly cross-link nucleic acids to nylon using UV light. This
makes Hybond-N ideal for any standard radioactive Southern or Northern blotting
procedure.
Ordering information
Product Quantity Code
7
PRODUCT RANGES
Hybond Atlas cDNA Expression Arrays
Hybond Atlas Array products represent a comprehensive range of high-density pre-
arrayed membranes that enable the expression of significant numbers of genes to be
screened simultaneously. These macroarrays each contain hundreds of carefully selected,
well characterized, arrayed cDNAs and offer the highest quality expression data.
Combining Amersham Biosciences' expertise in membrane hybridization and detection
with Clontech's expertise in expression arrays, Hybond Atlas Arrays are carefully
designed, pre-made macroarrays produced specifically for Amersham Biosciences by
Clontech Laboratories Inc.
Hybond Atlas Arrays provide sophisticated information, but are relatively inexpensive and
simple to use. They are designed to be accessible to all laboratories performing isotopic
blot hybridization experiments and optimized to be screened using RedivueTM nucleotides.
They are visualized using standard procedures, such as scanning with our range of
Typhoon™ Variable Mode Imagers, and are supplied with labelling, purification, and
hybridization reagents.
Hybond Atlas RNA Purification and Labelling Kit combines the purification of total
RNA and subsequent labelling of cDNA probes to be used with Hybond Atlas Arrays
in one experiment.
Ordering information
Product Quantity Code
www.amershambiosciences.com/hybondatlas
or by contacting your local office.
8
Hybond Northern Blots
Hybond Northern Blots are ready-made, ready to hybridize RNA blots for gene function studies:
• Analysis of the size and relative abundance of transcripts in different tissues
• Investigation of gene families and alternative splice forms
• Assessment of cross-species homology using different Hybond Northern Blots
There are five types of Hybond Northern Blot, each of which includes the highest
quality Poly A+ RNA from a variety of tissues or cell lines from a single species, either
human or mouse.
Ordering information
Product Quantity Code
Human a Northern Blot 1 kit RPN4801
Human b Northern Blot 1 kit RPN4802
9
HYBOND MEMBRANES FOR
A choice of three different Hybond formulations for Western blotting means we have the
right membrane for your application.
Hybond-P
A hydrophobic polyvinylidene difluoride (PVDF) membrane optimized for use in protein
transfers. With higher physical strength than unsupported nitrocellulose, Hybond-P offers
significant handling advantages and is ideal for reprobing. It is particularly recommended
for use with ECL Plus™ Western Blotting Detection Reagents and gives excellent results
with ECL Glycoprotein Detection System.
Ordering information
Product Quantity Code
10
Hybond ECL
An unsupported 100% nitrocellulose membrane that delivers excellent sensitivity,
resolution, and low background for all labelling and detection systems.
Ordering information
Product Quantity Code
Hybond ECL (82 mm) 50 discs RPN82D
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Hybond-C Extra
A supported mixed ester nitrocellulose with high physical strength. Hybond-C Extra
may be used in all protein transfer and detection procedures, but is particularly
recommended for expression screening because it gives high signal-to-noise and is easy
to handle.
Ordering information
Product Quantity Code
Hybond-C Extra (82 mm) 50 discs RPN82E
Hybond-C Extra (137 mm) 50 discs RPN137E
Hybond-C Extra (20 × 20 cm) 10 sheets RPN2020E
Hybond-C Extra (20 cm × 3 m) 1 roll RPN203E
Hybond-C Extra (30 cm × 3 m) 1 roll RPN303E
Ordering information
Product Quantity Code
Hybond Blotting Paper (46 × 57 cm) 100 sheets RPN6100M
Hybond Blotting Paper (20 × 20 cm) 100 sheets RPN6101M
Hybond Blotting Paper (23 cm × 100 m) 1 roll RPN6102M
Hybond Blotting Paper (19 cm × 100 m) 1 roll RPN6103M
Hybond Blotting Paper (27 cm × 100 m) 1 roll RPN6104M
12
FOR NUCLEIC ACID ANALYSIS
Nucleic acid purification
QuickPrepTM Total RNA Extraction Kit 27-9271-01
QuickPrep Micro mRNA Purification Kit 27-9254-01
GFX™ Genomic Blood DNA Purification Kit 27-9603-01
Labelling kits
RediprimeTM II DNA Labelling System RPN1634
Megaprime™ DNA Labelling System RPN1604
HotScribe™ First-Strand cDNA Labelling Kit RPN5650
AlkPhos Direct Labelling and Detection System RPN3690
ECL Direct™ Labelling and Detection System RPN3000
Gene Images Random Prime Labelling and Detection System RPN3500
ECFTM Random Prime Labelling and Detection System RPN5752
Ready-To-GoTM You Prime First-Strand Beads 27-9264-01
First-Strand cDNA Synthesis Kit 27-9261-01
13
PCR amplification
Taq DNA Polymerase RPN0303Z
Ready-To-Go PCR Beads 27-9553-02
PCR Nucleotide Mix US77119
Additional products
Rapid-hyb™ Buffer RPN1636
Ultrapure biochemicals
Restriction and modifying enzymes
Ultrapure solution dNTPs
14
FOR PROTEIN ANALYSIS
Blocking agents
ECL Blocking Agent RPN2125
Bovine Serum Albumin RPN412
Fish Gelatin RPN416
Liquid Blocking Reagent RPN3601
Secondary antibodies
Peroxidase-linked
Mouse IgG, HRP-linked Whole Ab from Sheep NA931
Mouse IgG, HRP-linked F(ab')2 Fragment from Sheep NA9310
Human IgG, HRP-linked Whole Ab from Sheep NA933
Rabbit IgG, HRP-linked Whole Ab from Donkey NA934
Rabbit IgG, HRP-linked F(ab')2 Fragment from Donkey NA9340
Rat IgG, HRP-linked Whole Ab from Goat NA935
Rat IgG, HRP-linked F(ab')2 Fragment from Goat NA9350
Anti GST, HRP-linked Polyclonal Ab from Goat RPN1236
Biotinylated antibodies
Mouse IgG, Whole Ab from Sheep, biotinylated RPN1001
Human IgG, Whole Ab from Sheep, biotinylated RPN1003
Rabbit IgG, Whole Ab from Donkey, biotinylated RPN1004
Rat IgG, Whole Ab from Goat, biotinylated RPN1005
Fluor-linked antibodies
CyDye Conjugates
Mouse IgG, Whole Ab from Sheep, Fluorescein-linked N1031
Rabbit IgG, Whole Ab from Donkey, Fluorescein-linked N1034
Mouse IgG, Whole Ab from Sheep, Texas Red-linked N2031
Rabbit IgG, Whole Ab from Donkey, Texas Red-linked N2034
Streptavidin-Enzyme conjugates
Streptavidin-Biotinylated HRP Complex RPN1051
Streptavidin-HRP Conjugate RPN1231
ECL Streptavidin-HRP Conjugate (500 ml) and Blocking Reagent (44 g) RPN2195
15
Detection reagents
ECL Western Blotting System RPN2108
ECL Western Blotting Reagents for 1000 cm2 membrane RPN2109
ECL Western Blotting Reagents for 2000 cm2 membrane RPN2209
ECL Western Blotting Reagents for 4000 cm membrane
2
RPN2106
ECL Plus Western Blotting Reagents for 1000 cm2 membrane RPN2132
ECL Plus Western Blotting Reagents for 3000 cm2 membrane RPN2133
ECL Plus Western Blotting Reagent Pack * RPN2124
*Does not contain detection reagents
ECL kits
ECL Glycoprotein Detection Module* RPN2190
ECL Protein Biotinylation System RPN2203
ECL Protein Biotinylation Module* RPN2202
ECL Phosphorylation Module* RPN2220
ECL GST Western Blotting Detection Kit RPN1237
*Order ECL Detection Reagents separately
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for further details contact your local office
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Germany Tel: 0761 4903 106 Fax: 0761 4903 405 Italy Tel: 02 27322 1 Fax: 02 27302 212 Japan Tel: +81 3 5331 9336 Fax: +81 3 5331 9370
Latin America Tel: +55 11 3667 5700 Fax: +55 11 3667 87 99 Middle East and Africa Tel: +30 (1) 96 00 687 Fax: +30 (1) 96 00 693
Netherlands Tel: 0165 580 410 Fax: 0165 580 401 Norway Tel: 2318 5800 Fax: 2318 6800 Portugal Tel: 21 417 7035 Fax: 21 417 3184
Russia & other C.I.S. & N.I.S Tel: +7 (095) 232 0250, 956 1137 Fax: +7 (095) 230 6377 South East Asia Tel: 60 3 8024 2080
Fax: 60 3 8024 2090 Spain Tel: 93 594 49 50 Fax: 93 594 49 55 Sweden Tel: 018 612 1900 Fax: 018 612 1910 Switzerland Tel: 01 802 81 50
Fax: 01 802 81 51 UK Tel: 0800 616928 Fax: 0800 616927 USA Tel: +1 800 526 3593 Fax: +1 877 295 8102
or visit us at:
www. amershambiosciences.com
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