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Occurs in flowering plants from structure called a perennating organ, which stores
enough nutrients to sustain plant during non-growing season and develop into 1 or
more new plants following year.
1. Use scalpel to take cutting from end of stem about 5cm-10cm long.
2. Remove leaves from lower end of cutting, leaving just one on tip.
3. Dip lower end of cutting in a pot containing hormone rooting powder to induce
root formation.
4. Plant cutting in pot with suitable growth medium, e.g. well drained compost.
5. Provide cutting with warm and moist environment by covering whole pot with a
plastic bag or putting in a propagator.
Advantages:
-rapid production - high yield in short space of time
-produce plants following genetic modification
-known pheno/genotype - used in selective breeding
-culturing meristematic tissue produces disease free plants
-alternate method to grow plants naturally difficult to propagate or are infertile
-plants can be reproduced at any time of year as controlled conditions maximise
growth and prevent disease
-less space needed
-produce seedless varieties of plants that consumers prefer
-reliable fast method to increase number of endangered species
Disadvantages:
-high production costs - labour intensive, skilled workers, high energy
-microbial contamination leads to large numbers of plants lost
-explants and plantlets vulnerable to mould during production
-no genetic variability - single disease or changing growing conditions could fail
whole batch
-undesirable characteristics always passed on
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Natural cloning in animals.
Only embryonic cells which are totipotent stem cells are naturally capable to
generate a new individual.
Invertebrates:
-fragmentation - from fragments of original
-budding - small buds on side of body
-binary fission - splitting in two
Vertebrates:
-monozygotic twins - embryo splits at early stages after conception
-pathenogenesis in amphibians - no male present to fertilise eggs
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Artificial cloning in animals - artificial twinnin
Artificial twinning - young embryo, ball of totipotent cells, is split to create 'articulate
identical twins', using the same principle of natural twinning.
Uses include rapidly increasing herd size of farmed animals with favourable
characteristics.
Advantages:
-AT - produces many offspring from high yielding farm animals
-SCNT - enables GM embryos to be relicated and grown to give multiple embryos
from single engineering procedures
-helped develop new treatments
-increase population of enedangered species
-clones animals anytime and anywhere
-clone infertile animals
Disadvantages:
-lower life expectancy
-very difficult, time consuming and expensive
-no gentic diversity so undesirable characteristics past on
-low success rate in increasing population of rare organism or allowing extinct
animals back to life
-cloned embryos often miscarry or form malformed offspring
-inefficient - many eggs produce single offspring
-controversial using human embryos
-animal welfare issues
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Ethical concerns of gene manipulation.
GM soya bean - inserted gene to products Bt protein which is toxic to many pest
insects and be resistant to weed colour:
-reduced chemical pesticide used and increases yield
-insects may become resistant to pesticides in crops
-encourages monoculture
-transferred genes spread to wild producing super weeds
-people in LEDCs prevented from using them by patents and issues of technology
transfer
Pathogens for research - genetically modified to find treatments for disease:
-making untreatable diseases treatable reducing suffering
-protenital infection with life pathogen leading to outbreak of disease
-worry it gets into wrong hands and used by wrong hands to create agents for
biowelfare
Pharming - genetically modified animals produce pharmaceuticals by creating
animal models (additions or removal of genes so animals develop certain diesease
and creating human proteins (intro of human gene coding for medically required
protein):
-drugs made in larvae quantities
-harmful animal side effects and enforces idea that animals are just asssests
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Gene therapy.
Cures genetic diseases caused by inherited abnormal genes by altering genes inside
cells by either supplementing faulty genes with a dominant allele or silencing a
dominant allele by inserting DNA in the middle. New alleles inserted into cell by
vectors.
Somatic therapy:
-replacing mutant allele with healthy allele in affected somatic cell
-viral vectors often used
-treating retinal diseases, immune diseases, leukaemias, myeloma and haemophilia
-temporary solution - somatic cells have limited life so are replaced with stem cells
and faulty alleles still placed to children
Free enzymes are often very wasteful and not cheap because at the end of the
process they cannot usually be recovered and are lost.
Immobilised enzymes - enzymes attached to an inert and insoluble support system
over which substrate passes and is converted to product (a case of technology
mimicking nature - enzymes in cells usually bound to membranes to carry out
repeated cycles of catalysis).
Advantages:
-reusable - cheaper
-easily separated from reactants and products of reaction so reduced downstream
processing so is cheaper
-more stable - less likely to denature as greater temperature tolerance so bioreactor
is cheaper to run
-more reliable - more control as support provides stable microenvironment
-ease of manipulation - catalytic properties can be altered to fit process more easily
Disadvantages:
-reduced efficiency - reduced rate of activity as not mixing directly with substrate
-higher initial material costs - more expensive than microbes and free enzymes
-high initial bioreactor costs - conditions different from traditional fermenter
-more technical issues - more complex reactors
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Methods of immobilising enzymes.
Surface immobilisation - adsorption to inorganic carriers e.g. silica and carbon
nanotubes with partially permeable membrane for substrate.
+simple, cheap and can be used with many different processes
+enzymes very accessible to substrate so activity virtually unchanged
-enzymes easily lost from matrix
Surface immobilisation - covalent or ionic bonding to inorganic carries.
+variable cost and enzymes strongly bound so not lost
+enzymes accessible to substrate
+pH and [substrate] has little effect
-variable cost and active site of enzyme may be modified so less effective
Entrapment/inclusion - in matrix e.g. gelatin, polysaccharides.
+widely applicable to different processes and enzyme in natural state
-expensive and difficult, may effect enzyme activity depending on matrix
-diffusion of substrate to and product from active site slows rate
Membrane entrapment - in micro capsules or behind semi-permeable membrane.
+simple and widely applicable with small effect on enzyme activity
-expensive and diffusion to and from active site can slow rate
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Immobilised microorganisms.