Scribd Logo
Загрузить

Добро пожаловать в Scribd!

  • Beetroot Pigments 1210

    Загружено:

    hassbanasghar39
    16 просмотров2 страницы
    This experiment aims to examine the effect of temperature on cell membranes using beetroot. The hypothesis is that higher temperatures will increase cell membrane permeability. Beetroot slices will be placed in water at different temperatures, and the concentration of leaked pigments will be measured using a colorimeter. Replicates and a control will be used to ensure accurate and reliable results. The dependent variable is absorbance, the independent variable is temperature, and other variables include the size and concentration of beetroot slices and water bath temperature.

    Исходное описание:

    Оригинальное название

    Beetroot pigments 1210

    Авторское право

    © © All Rights Reserved

    Доступные форматы

    PDF, TXT или читайте онлайн в Scribd

    Этот документ был вам полезен?

    Это неприемлемый материал?

    Пожаловаться на этот документ
    This experiment aims to examine the effect of temperature on cell membranes using beetroot. The hypothesis is that higher temperatures will increase cell membrane permeability. Beetroot slices will be placed in water at different temperatures, and the concentration of leaked pigments will be measured using a colorimeter. Replicates and a control will be used to ensure accurate and reliable results. The dependent variable is absorbance, the independent variable is temperature, and other variables include the size and concentration of beetroot slices and water bath temperature.

    Авторское право:

    © All Rights Reserved
    Скачайте в формате PDF, TXT или читайте онлайн в Scribd
    Отметить как неприемлемый контент
    16 просмотров2 страницы

    Beetroot Pigments 1210

    Загружено:

    hassbanasghar39
    This experiment aims to examine the effect of temperature on cell membranes using beetroot. The hypothesis is that higher temperatures will increase cell membrane permeability. Beetroot slices will be placed in water at different temperatures, and the concentration of leaked pigments will be measured using a colorimeter. Replicates and a control will be used to ensure accurate and reliable results. The dependent variable is absorbance, the independent variable is temperature, and other variables include the size and concentration of beetroot slices and water bath temperature.

    Авторское право:

    © All Rights Reserved
    Скачайте в формате PDF, TXT или читайте онлайн в Scribd
    Отметить как неприемлемый контент
    из 2

    '~~

    "

    ~ ..

    ~Ji) '2-c64'I
    PRACTICAL - BEETROOT PIGMENTS

    AIM: To use beetroot to examine the effect of temperature on cell membrane~ and relate the
    effects observed to membrane structure.

    - ------------
    ,>"..
    iI'
    HYPOTHESIS: (what do you:t,~~;}\',l.l1peJbe effect of temperature on cell surface
    ~brane,~(f!n '";':"'~ci~tthi~,~ffecttbeir permeability?)
    * hf~Ler1rtT}p
    ~~.
    '. /'

    ."-",~,.,,,.
    .W' ~~<,JfR¥C'2..,;.
    "
    '

    ,.
    , ~ pmel:r
    .. .. ~'~
    .
    .:'tY)oCt pern-Jeab\e...-
    .,
    .

    I
    * 10 ~ l.tJiH .\>~. +~e. ""cif:"?t~~~' .

    ~ -rhl:! crs \ef\ec1ed iV/' \:~t: h\'e~es~ (1'pr\)rp~O\" ,/

    APPARATUS LIST:
    r'~

    . Raw beetrootl ci)rklj'qrer white tile .-", scalpel

    . Ruler 250~nrg1assb~er forceps boiling tubes


    I
    colorimeter cuvettes
    . 'Water batbs at 0 -70oCtherylOmeter
    \

    . Stopwatch distill~{ ~ater pipette measuring cylinder

    METHOD ~

    ~~~~ 1-
    L Cut 24)lcm length slices ITombeetroot using a cork borer and place in a beaker of
    distilled water. Leave for 5 minutes.

    2. Place 1 slice into each of3 boiling tubes containing 5cm3of distilled water and place in a
    beaker of water placed in ice. Leave for 20 minutes.

    '.3, Remove the beetroot sections and shake the boiling tube to disperse the dye.
    .\

    4" Set the colorimeter to read % absorbance and set the filter dial to the blue-green filter.
    , --

    /~ - "'""
    ".,.
    ~
    """

    5. Using a pipette, measure 2cm3of distilled water into a cuvetty and place in the
    colorimeter. Adjust it io rearlzero absorbance for distiIIedwater.

    6. Place 2,~m3of the dye solution ITomone of the 3 boiling tubes into a~uvette and take a
    reading for absorbancy.Repeat with the other 2 slices. Calculate an average.

    7. R~peat the readings for aIlthe.teI;l1perature~- '"


    f,\nat 6kf

    - 1~'\'~~~~S~
    RIS~ MENT(ide~iify~~y.;;i~ici:'~d"1ist
    wo..t(r'e
    safety precautions)
    ' ,

    - \i,Leo:(a~~'
    eJ
    - $(V:f\tL- ct..\A hard! !
    - \s-IQa.'\8 \ 0 'J€.I
    - ~uk of S\-O..H1\'hJb..l\ O,~u~d/ Lv!\-\ bJrb"\' J'W{t.

    RE~IABffiJTY ANJ) ACC~~~i!2¥;~~~ULTS (how willJ?tfensure that the results are


    . aCC1J1:ate
    anp reliable? How will YOP~~ii$Uf,~;tbat
    ,
    measurements are' " ,~~'(.0i'\,""", ,<°. ;;;.'"

    .~precise, acclp"ate and repeatable?~~~uaereplicates and use of a control). co - '. l-


    ¥oJ(4(~\t ~U\T't '0\ wo..\c1 \1'\(~fJ.tt.~... ;.t(I¥( ff~~ " (w (t{1 G\~( ea~ re}

    ~ i7;r~.u-e. 1\'0\1"<1 l-c l"'e (""V~ ! \,;


    \
    \ 0 \) /( -\'i.,!',
    \Je. {'G'T1~ I'2 01< JJ s\ill~ l..d.\~Y' '; , }'( ,'( u~ 'I (11<

    ':!C So. /ii(. (e~ \.l k:-ci rcof.


    1': 5ollV {1oSf 5r(~EhOI .51(t\ J J;{d ,DC-r (Off'
    ¥~. (tpe4.~J
    \
    4:{ \01 D(7~!
    I "I
    ro;; J leJCI.~
    ~ )'C.;K ~'I)'( .'" L--II4\,,' ~,,~cl1 ,. -
    IDENTIFICATION OF DEPENDENT AND INDEPENDENT VARIABLES,

    1. DEPENDENTVARIABL~= le ~ ~ ,
    2. INDEPENDENT VARIABLE = ab.£O-(pt1CJIJ
    3. OTHER VARIABLES = -VD"-\))'\{' s.~\L1+{ov)
    . J-i()~ {n. wo..L'('v: 'tx:tth

    - \-€Y)5~\:-'
    . ofL bee:\. (bOr f .;' I
    l~( beef '(O-Jr'
    - (fOr;.!
    \ <)~c \1°I'\(). G,'( (1C\. 0, . .

    - (o\cuc o~ft.'l<,rf

    Вам также может понравиться