TC220 Manual Tenico V1.15

TC220 Chemistry Analyzer Technician Manual(Ver1.
15) YeMengRui
TC 220
Automatic Chemistry Analyzer
Technician Manual
Ver. 1.15
Tecom Science Corporation
555# Gao Xin Ave., Nan Chang National
Hi-tech Industry Development Zone, Nan Chang,
Jiang Xi 330096, P. R. China
Tel.: 0086-791-88116898
Fax: 0086-791-88111989
Web: www.tecom-cn.com
Email:service@tecom-cn.com
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TC220 Chemistry Analyzer Technician Manual(Ver1.15) YeMengRui
CONTENT
Chapter 1 Analyzer Introduction ···········································································································4
1.1 System Structure······························································································································4
1.1.1Appearance pic of Analyzer ···································································································4
1.1.2 Composition of Analyzer ·······································································································6
1.1.2.1 Sample/Reagent Tray Assembly ·················································································6
1.1.2.2 Reation Tray Assembly and Temperature Control System·····································10
1.1.2.3 Photometric System ··································································································12
Chapter 2 Installation ························································································································12
2.1 Unpacking ·······································································································································12
2.2 Installation Environment Requirements ·······················································································13
2.2.1 Installation Environment Requirements ·············································································13
2.2.2 Power Requirements············································································································14
2.2.3 Temperature and Humidity Requirements ·········································································15
2.2.4 Space Requirements ············································································································15
2.3 Installation Process························································································································15
Chapter 3 Fluidic System·····················································································································28
3.1 TC220 Analyzer Fluidic System·····································································································28
3.1.1 Fluidic Diagram ····················································································································28
3.1.2 View Pictures of Analyzer Parts ··························································································29
3.1.3 Fluidic system steps instruction·························································································31
Chapter 4 Hardware Structure·············································································································33
4.1 Sample /reagent tray ······················································································································33
4.2 Reaction tray and drive assembly ································································································33
4.3 Optical Sensor ································································································································35
4.4 Sample/Reagent probe and drive assembly ················································································36
4.5 Mixer probe and drive assembly ···································································································37
4.6 Sample/reagent syringe and drive assembly···············································································38
4.7 7-steps washing unit and drive assembly ····················································································38
4.8 Photometric System·······················································································································39
4.9 Temperature control process of Reaction Tray ···········································································41
4.10 Water Temperature Control process of water tank····································································43
4.11 System Power Supply ··················································································································44
4.12 Circuit board function description······························································································46
4.12.1 Main controller board function··························································································46
4.12.2 Bus board····························································································································48
4.12.3 Signal Process Board ········································································································49
4.12.4 Liquid Level Detection Board····························································································50
4.12.5 Power Board ·······················································································································52
4.12.6 Water Controller Board ······································································································53
4.12.7 Reagent Refrigeration Pinboard ·······················································································54
4.12.8 M7 Motor Subdivision Board·····························································································55
4.12.9 Stepping Motor Drive Board······························································································56
4.12.10 Mixer Motor Power Board ································································································58
Chapter 5 Component Adjustment and Replacement·······································································59
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5.1 Signal process board voltage adjustment····················································································59
5.2 Reaction tray position adjustment································································································60
5.3 Tungsten-Halogen Lamp replacement··························································································61
5.4 Sample/reagent probe position adjustment·················································································63
5.4.1 Wash well position for sample/reagent probe ···································································64
5.4.2 Cuvette position for sample/reagent probe ·······································································65
5.4.3 Sample position for sample/reagent probe········································································66
5.4.4 Reagent position for sample/reagent probe ······································································67
5.5 Sample/reagent probe replacement······························································································69
5.6 Mixer probe position adjustment ··································································································70
5.6.1 Wash well position for mixer probe ····················································································71
5.6.2 Cuvette position for mixer probe ························································································72
5.7 Mixer probe replacement ···············································································································73
5.8 Synchronous belt replacement ·····································································································75
5.9 Light filter replacement··················································································································75
Chapter 6 Maintenance ························································································································80
6.1 Daily Maintenance ··························································································································80
6.1.1 Check the distilled water volume of water bucket·····························································80
6.1.2 Check waste solution···········································································································80
6.1.3 Check the signal value of all cuvettes················································································81
6.1.4 Check sample/reagent probe clogging ··············································································81
6.2 Weekly Maintenance ······················································································································82
6.2.1 Clean sample/reagent probe and mixer probe···································································83
6.2.2 Clean two wash well·············································································································83
6.2.3 Clean sample and reagent tray ···························································································84
6.2.4 Clean the reaction tray·········································································································84
6.2.5 Clean 7-steps washing needles ··························································································84
6.2.6 Clean cuvettes using wash solution···················································································84
6.2.7 Check the signal value of all cuvettes.···············································································85
6.3 Monthly Maintenance ·····················································································································86
6.3.1 Clean incubation groove of reaction tray···········································································86
6.3.2 Clean the panel of machine ·································································································87
6.3.3 Clean distilled water bucket ································································································87
6.3.4 Clean waste solution bucket ·······························································································88
6.3.5 Clean water filter ··················································································································89
6.3.6 Clean dust screen ················································································································89
Appendix A Spare Parts Price List ······································································································91
Appendix B Preventive Maintenance Kit ····························································································97
Appendix C Error Code Message········································································································98
Appendix D Diagram ····························································································································99
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Chapter 1 Analyzer Introduction
1.1 System Structure
TC220 biochemistry analyzer is composed of analyzing module and operation unit.
1.1.1Appearance pic of Analyzer
Upper cover
（Front View）
Sensor and Fluidic connector interface
(Left View)
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Cooling and power switch

Male 9-pins serial port
Female 9-pins serial port Power inlet
Earth line connector Fuse
（Right View）
Air outlet
Air inlet
(Rear View)
（Planform view closing analyzer’s upper cover）

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Sample/reagent
probe assembly Mixer probe assembly
7-steps wash unit
Reaction tray
Reagent tray
Wash well of sample/reagent probe

Sample tray
（Planform view after opening analyzer’s upper cover）
1.1.2 Composition of Analyzer
TC220 main unit is mainly consists of sample/reagent tray assembly 、 reaction tray
assembly、temperature control system、optics system etc.
1.1.2.1 Sample/Reagent Tray Assembly
Reagent
position
Sample
position
（Sample/reagent tray assmebly pic）
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● Function and Composition
Sample/reagent tray assembly including sample and reagent tray 、 sample/reagent
dispenser system and related mechanical parts ,its function is made sample and reagent to
be dispensed to the correct cuvette position which is controlled by software.
Sample/Reagent Tray
● Specification
Sample tray has 18 sample positions
Sample tray
Reagent tray has 25 reagent positions,The reagent bottle volume is 20ml.NO.1 reagent
position is defined by software for distilled water bottle.it is used for predilution or
post-dilution.
Reagent tray
When you load reagent bottle,it must be attention to make bottom of bottle to be on a level
with bottom of sample/reagent tray ,don’t appear the different height.The reagent bottle
volume is 20ml.
Attention
● Don’t load/unload sample cup or reagent bottle when the sample/reagent is running.
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● Sample cup is one-off consumables,cann’t reuse ,otherwise it will be appear carryover.
Sample/Reagent Dispenser System
Probe rotor Sample/reagent probe
（Sample/reagent probe mechanical assembly pic）
Sample/reagent
syringe assembly
● Function and Composition
Sample/reagent dispenser system is consist of sample/reagent probe、 rotor mechanical
assembly、reagent/sample syringe assembly,carry sample or reagent to reaction cuvette.
● Specification
Sample volume range：1～50ul.
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Reagent volume range：10～400ul.
● Motion Specification
Sample/reagent dispenser mechanical system：it moves again and again with the sequence
which is sample/reagent tray→reaction tray→wash well and finishs adding sample or
reagent into the reaction cuvette.
Liquid level detection：sample/reagent probe have auto liquid level detection funtion，it is
suitable for different liquid level height sample or reagent.
Crash protection (vertical & horizontal)：When the probe crashs in horizontal or vertical
direction,the system will alarm.
Reagent refrigeration system：reagent refrigeration system starts to work when the cooling
switch is on.
Attention
When the analyzing unit is in operation, do not place any part of your body or any obstacle in the route the
arm moves. Otherwise, it may lead to personnel injury or equipment damage.
Mixer System
Mixer arm
Mixer probe
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● Function
It is used to stir the reaction liquid (reagents and sample) in reaction cuvette to make them
react completely.
● Motion
When stirring is finished, the mixer moves automatically to its wash well for cleaning.
For single-reagent test, the sample mixer starts to work once sample is dispensed.
For double-reagent test, the reagent mixer starts to work once R2 is dispensed.
1.1.2.2 Reation Tray Assembly and Temperature Control System
Tray lock knob
Reaction cuvette
holder lock knob
(Reaction tray assembly pic)
Reaction cuvette
holder lock knob
（Reaction cuvette holder pic）

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● Composition and Function
Reaction tray assembly is consist of reaction tray and temperature control parts.
Reaction tray is used to load reaction cuvettes. Reaction cuvette is as reaction liquid
place,meanwhile,it is as colorimetric measurement optics device .When reaction cup pass
through the optics route,the analyzer measures absorbance of reaction liquid.
Temperature control is used to offer a homothermal environment for reaction.
● Specification
Reaction tray：using 6 reaction cuvette holders to load total 60 reaction cuvettes，each
holder loads 10 reaction cuvettes.
Temperature control parts：via heating loop and temperature sensor to make the reaction
temperature is in the 37±0.1℃.
Reaction cup size：5mm×6mm×25mm (L×D×H),，optical path 6.1mm.
● Motion
Reaction tray only make counterclockwise rotation。Reaction tray make appointed reaction
cuvette stop in adding sample position、adding reagent position and measure optical axis
position.
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1.1.2.3 Photometric System
● Composition
Photometric system includes tungsten-halogen lamp、reaction cuvette(colorimetric cup)、

optical fiber、optical filter、signal process board and so on.
● Specification
9 wavelengths, which are：340nm、405nm、450nm、510nm、546nm、578nm、620nm、

660nm、690nm；
Wavelength precision：±1.5nm；
Photometry range：0.0000-4.0000ABS；
Optical source：12V,tungsten-halogen ,20W；
Chapter 2 Installation
Attention
The system should be installed by our authorized personnel only.
The system should be installed by our authorized personnel only, and you should prepare a
proper site for installation.
If you need to move the system to another site, please contact our Customer Service
Department or your local distributor, who are the appropriate people for the moving job.
2.1 Unpacking
When you receive the system, carefully inspect the package. If you see any signs of
mishandling or damage, file a claim immediately with our Customer Service Department or
your local distributor.
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After opening the package, check the delivered goods against the packing list as well as
the appearance of the system. If you find anything missing or damaged, alert our Customer
Service Department or your local distributor immediately.
2.2 Installation Environment Requirements
Attention
Make sure the system is installed in a place meeting the following requirements. Otherwise,
it will not perform as promised.
2.2.1 Installation Environment Requirements
⊙ The system is for indoor use only.
⊙ The bearing platform (or ground) should be level (gradient less than 1/200).
⊙ The bearing platform (or ground) should be able to bear 30Kg weight.
⊙ The installation site should be well ventilated.
Attention
The system radiates heat while operating. A well-ventilated environment helps keep the
room temperature stable. Use ventilation equipment if necessary. But if so, be sure not to
expose the system to the direct draft that may lead to unreliable results.
⊙ The installation site should be free of dust as much as possible.
⊙ The installation site should not be in direct sun.
⊙ The site should not be near a heat or draft source.
⊙ The installation site should be free of corrosive gas and flammable gas.
⊙ The bearing platform (or ground) should be free of vibration.
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⊙ The site should not be disturbed by large noise or power supply.
⊙ The system should not be placed near brush-type motors and electrical contacts that are
frequently turned on and off.
⊙ Do not use such devices as mobile phones or radio transmitters near the system.
Electromagnetic waves generated by those devices may interfere with operation of the
system.
2.2.2 Power Requirements
⊙ Power supply: AC 100-240V, 50/60Hz, with voltage fluctuation of ±10%.
⊙ Three-wire power cord, which should be grounded properly.
⊙ The system should be connected to a properly-grounded power socket.
⊙ The distance between the power socket and the system should be less than 2.5 meters.
⊙ Ground voltage must be configured.
⊙ The equipment’s maximum output is 350 W, customizable with 1000W on-line UPS.
Warning
Make sure the power socket is grounded correctly. Improper grounding may lead to electric
shock and/or equipment damage.
Be sure to connect the system to a power socket that meets the above-mentioned
requirements and has a proper fuse installed.
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2.2.3 Temperature and Humidity Requirements
Ambient temperature: 10-35 centigrade, with fluctuation less than ±2 centigrade/H.
Relative humidity: ≤90%RH , without condensation.
Attention
Operating the system in an environment other than the specified may lead to unreliable test
results.
If the temperature or relative humidity does not meet the above-mentioned requirements,
be sure to use air-conditioning equipment.
2.2.4 Space Requirements
The system should be installed and used meeting the space and accessibility requirements
as shown below.
wall
Minimum500
Maximum2000
Analyzer PC
383
548 Unit:mm
Front
2.3 Installation Process
1.Make sure that in the hospital, there is a proper installation area with qualified
environments.
2. Confirm installation reagent& QC.
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3. In the installation place, unpack the package, check the goods according the packing-list
and ask customer to inspect&accept.
4. Place the analyzer on the proper location.
5. Install computer, printer and then test the printer whether it can work normally.
Attention
The TC220 software is better used in the Windows XP and WIN7 operating system.
6. Inspect the equipment appearance to see whether it is OK. Open the top cover, check
whether sample/reagent probe assembly, mixture probe assembly,reaction tray assembly
and another assembly are ok, removing related protective foam .
7. Holding the sample/reagent probe assembly arm, pull several times up and down & left
and right respectively at wash well position, reaction cup position, reagent position, sample
position and other positions around, to see whether the movement resistance is uniform
and smooth in all directions.
8. Connect serial port communication cable, equipment power line, ground wire, distilled
water bucket, waste-liquid bucket and related sensor cables.
9. Fill up the distilled water bucket with distilled water,then connect the distilled water
sensor subassembly with machine.
Water tube
Water sensor
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Distilled water sensor subassembly
Waste sensor
Waste tube
Waste sensor subassembly
When install the machine,please put water and waste bucket on the lower level place than
machine.otherwise water will flow out from machine.the connection pictures are shown as
followings.
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Tube connection
Water bucket
Waste bucket
10. Check whether the 60pc reaction cuvettes are damaged in the reaction tray. Please
observe whether each reaction cups height are at the same level on the reaction tray.
Remarks:
The reaction tray is consisted by 6 connected cup holders and each holder is loaded by
10pc reaction cuvettes.
11. Please open the power switch as follow: analyzer power switch→computer power
switch→external printer power switch；
12. Copy the Chemistry analyzer installation software from installation CD into the
computer’s hard disk D drive or E drive.
Attention
① C drive is infected more easily by computer virus than another drives.
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② There is a piece of USB to serial port convertor in the accessories of machine,and if
there is not 9-pins serial port in the computer,the convertor should be used,otherwise it is
not needed.
Refer the following steps to install the convertor.
Step1: there are two ports in the USB convertor.one is USB port.the other is male 9-pins
serial port.
The communication serial cable of

the analyzer
USB Convertor cable
Step 2: connect the serial cable with male 9-pins port of convertor,and connect the USB
port of convertor with computer.
Step 3: The computer will indicate the new hardware is found.
Step 4: Insert driver CD of USB convertor into CD drive.and then the automatic installation
window is appeared.and close the window through click cross button.
Close the window
19
Step 5: The screen will indicate “Found new hardware wizard” see the following picture.
then always click next button to perform next process.
20
Step 6: Finish the USB convertor cable driver installation.
21
Step 7: after install the driver of convertor,enter the hardware information menu which is in
the control panel to check whether the added com number is 1 to 3 or not.if not,it
should be done to modify it through advanced properties menu of the added com
port.
13. There are two folders in the installation CD , one folder is “TC200_V43_EN” which is
operating software folder, the other folder is “PIC”.there are three factory setting menu
pictures in the PIC folder.
Attention
① There is some important factory setting information in the CD,if you change the
computer or reinstall the windows operating system,you should set some menu again.so
you should keep the installation CD carefully.
22
② Each machine have own factory setting pictures,so it is necessory to keep each
machine’s installation CD.
14. Open the “TC200_V43_EN” folder and double click the icon of TC200 application
program which is .then select login ID “Admin” and input “Admin” password to
enter the software operation interface.
Note:in the original software ,the default login ID have two ID.one is Admin,the password is
Admin.the other is 1,the password neednot be inputted.
15. Enter “Maintenance”→ Parameters setting →input password“777”,set up related
parameters.
23
Parameter Meaning
Com Serial Port It is the serial port number connected between machine and computer.
Select Generally it is automatically detected by software.
Language Normally choose English.
Barcode Length Generally, the item should be set up by engineers according the Barcode requirement.
Separate Mixing TC220 haveseparate mixing system. select “Mixing system”
System
Choose “Sample First” to test according samples orders.

Test Order Choose “Item First” to test according Items orders.
Normally we advise customer to choose item first in order to avoid items’ effect.
QC range According QC target sheet which supply SD or Range.
If select “power on run”,when customer enters the operating software,the machine automatically
Power On Wash
washes all reaction cuvettes.
If select “power off run”,when customer exits the operating software,the machine automatically
Power Off Wash
washes all reaction cuvettes.
Screen Color Click “Back color” button to choose background color for software operating interface according
Setup customer’s favor.
Syringe Drive Only select “Drive Board”,otherwise sample/reagent syringe movement is abnormal.
Mode The drive board is M7 Motor Subdivision Board and refer chapter “Circuit board function description”.
Result too small If the result is more less than lower limit of reference range,the
parameter will be retested.
Balance has not been If the blank absorbance is not stable,the parameter will be retested.
Auto-check tested
setup Beyond the scope of If the result is beyond upper limit of linearity range,the parameter will be
linear retested with auto-dilution.
Substrate depleted If substrate is out, the parameter will be retested with auto-dilution.it is
valid for kinetic and two-points method.
Server IP According the LIS system setting.
If the sample/reagent probe cannot detect the corresponding reagent, the machine donot test the
corresponding parameter in the next measurement cycle.
Rgt.Sample
If the sample/reagent probe cannot detect the sample some times,the machine donot test the sample
Alarm
in the next measurement cycle.this time can be set in the “instrument settings”menu.its name is
serum times.normally we set four times.
16. Click “Initialize” to observe the Initialization status of each parts and the lamp should
facus the light point into the NO.11 cuvette.otherwise you should adjust the reaction tray
position refer “component adjustment and replacement”.
24
17. Make the water tank fill with distilled water .
Attention
① If the water tank can’t be injected water, please unscrew the air plug which is on the left
side of analyzer about 20 seconds.Screw the air plug tightly when the water arrives at
proper level in the water tank.
② Donot test CRU wash valve in the Motion detection menu,if you click it,the water will be
dispensed into cuvettes in a very short time,and then flow out cuvettes and flow into the
incubation groove.
18. Input password “999” to enter “Instrument settings” menu. Then confirm the setting
values is the same with factory picture .
25
Attention
There are one sample/reagent probe and one mixture probe in the TC220,arm setup
colume and stiring setup colume are used to adjust probe’s positions.if positions are not
good.engineer should adjust it.
19. Enter “Temperature pressure” menu. Then confirm the setting values is the same with
factory picture .
20. Enter “Cuvette cleaning” then perform washing all cuvettes three times.
21. Enter cuvettes checking menu, click “Add water” and then “check cuvettes” to get the
signal value , observe each wavelength signal value ,it should be between 30000-56000,
If the signal value is lower than 30000 or more than 56000, please check related
wavelength cuvettes.
26
22. To drain water of cuvettes by click “remove water”.

23. First setup parameters of ALT(Rate),TC(Endpoint),UREA(Two points),
then do some tests with QC and clinic samples,check whether the results are correct and
repeatable.
24. To give operators training, make sure that operators know and can operate followings
procedures::
① Daily test process
② Parameter setup, Calibration and QC
③ Test methods, including Rate, Endpoint and Two points.
④ Daily analyzer maintenance
⑤ Replace sample-reagent probes, mixers, light source and reaction cuvettes.
⑥ Printout format setup
⑦ Other menus setup
25. Fill in installation report of Inspection and Acceptance, ask hospital to make signature
with officer seal and leave business card to them.
26. Complete Installation
27
Chapter 3 Fluidic System
3.1 TC220 Analyzer Fluidic System
3.1.1 Fluidic Diagram
28
Water dispensing valve

Outer probe washing valve
Inner probe washing valve
Backwater peristaltic pump
Drain pump
Sample/reagent syringe
Attention
Before you detect CRU wash valve(water dispensing valve),you should put one empty cup
under the washing needles,otherwise some water will be dispensed into cuvettes in the
short time,and then overflow into the incubation groove.the overflow water will damage the
rotating stepping motor of reaction tray.
3.1.2 View Pictures of Analyzer Parts
Signal Process board

Main Controller board
Four pieces of stepping

Water controller board motor drive board
（Front View）
29
Power board
Switch power supply
（Right View）
Water pump
Water tank
Reagent Tray Refrigeration Sample/reagent

Pinboard syringe Subassembly
（Left View）
30
Separatory Liquid Block
Backwater valve
Drain pump
Backwater pump
（Rear View）
3.1.3 Fluidic system steps instruction
1．Water pump1(P3) and water pump2(P4)to take distilled water into water tank, and when
the liquid is not enough, it will take in more distilled water.
When the liquid level of water tank reaches certain height, air valve(EV4) will open to take
in air. Then when the pressure and liquid level reach certain level and keep constant, water
pumps will stop working.
The liquid level of water tank is controlled by its inner float switch whereas the pressure is
controlled by the pressure sensor connected to it.
2．The washing procedure of reaction cuvettes.
2.1 When the seven washing needles goes down to the bottom of reaction cuvettes, the
drain pump(P0) will be on ,then aspirate and pump water from cuvettes.
2.2 Water dispensing valve(EV1) is opened, pressure in water tank makes the distilled
water of tank dispense into reaction cuvettes from five short washing needles through water
filter. at the meantime, backwater pump(P1) and backwater valve(EV5) are working ,then
31
pumpback completely the water of front end five short washing needles and prevent water
dropping from washing needles.
3. The movement of sample/reagent probe aspirating reagent&sample and rinsing:
Sample/ reagent probe rotates above the reagent bottle or sample serum cup, and
sample/reagent syringe goes down to aspirate certain volume of reagent&sample, then
sample/ reagent probe rotates above the reaction cuvettes, and then goes down into it, and
the syringe goes up to dispense reagent&sample into reaction cuvettes.
Then sample/reagent probe goes up and rotates to washing well, EV2 and EV3 will be
opened, the water which is from water tank wash the inner and outer side of
sample/reagent probe and the inner space of sample/reagent syringe.
Note: there is different sample/reagent probe movement route according single or double
reagent.
Single reagent : Wash well→Reagent aspiration→Wash well→Sample aspiration→
Reagent&sample dispension to reaction cuvette →Wash well
Double reagents:Wash well→R1 reagent aspiration→Wash well→Sample aspiration→
Reagent&sample dispension to reaction cuvette→Wash well→R2 reagent aspiration→
Wash well→R2 reagent dispension to reaction cuvette →Wash well
4. The movement of mixer probe and rinsing:
Mixer probe rotates above the reaction cuvettes, and then goes down into it for mixing,and
then move to wash well for cleaning.
32
Chapter 4 Hardware Structure
4.1 Sample /reagent tray
● Sample tray have 18 sample positions.reagent tray have 25 reagent positions.
● Refrigeration system is consisted by two refrigeration semiconductores and two
cooling fans.one side with words of semiconductor is cold side.the hot side of
semiconductor is pasted with heat dissipator.the cold side of semiconductor is pasted
with the bottom of reagent tray.two cooling fans release the heat which come from the
hot side of semiconductor.
refrigeration semiconductor
（hot side）
Size:30×30×3.5
Refrigeration semiconductor
（cold side）
4.2 Reaction tray and drive assembly
Reaction tray have 60 cuvettes which supported by six cuvette holders with each holder
have 10 cuvettes.It was forbidden to touch transparent surface of the cuvette.
Cuvette position is controlled by code disk(61 slots) and optical sensor.
Temperature control of reaction tray:the heating loop which is on the bottom of incubation
33
groove heats the incubation groove. Temperature sensor and temperature controller board
control temperature of reaction tray in about 37℃.
The type of stepping motor which control the reaction tray rotation is 23HY109-20B2.
The size of rotation synchronous belt is 119MXL(width 6mm).
Initialization slot
Code disk
Optical sensor of reaction tray

Type:ST151
Initialization slot
When you click initialization button which is in the operation software, the reaction tray
rotates until the initialization slot of code disk is detected by optical sensor.the light of
tungsten-halogen lamp focus the middle of NO.11 cuvette.
the heating loop which is installed on the bottom

of incubation groove
34
Heating loop with temperature

switch
Temperature switch KSD9700

Type:KSD9700 60℃
250V 10A
4.3 Optical Sensor
Optical sensor is used to make main controller board know the position of mechanical part
movement.if the optical sensor is not working well,the corresponding mechanical part
movement will be in disorder.
1.optical sensor of reaction tray rotation
Optical sensor of reaction tray.

Type:ST151
It is used to detect position of cuvette
2.optical sensor of sample/reagent syringe vertical movement
35
Optical sensor of sample/reagent syringe

Type:ST157
It is used to detect upper position of
syringe piston.
3.optical sensor of sample/reagent probe vertical and horizontal movement.
Optical sensor of sample/reagent probe vertical movement

Type:TP808
It is used to detect upper position of sample/reagent probe.
Optical sensor of sample/reagent probe horizontal movement

Type:TP808
It is used to detect horizontal position of sample/reagent probe.
4.optical sensor of washing unit vertical movement
Optical sensor of washing unit vertical movement

Type:TP808
It is used to detect upper position of washing unit.
4.4 Sample/Reagent probe and drive assembly
Specification：
The type of stepping motor which control the probe to be up and down or left and right is
17HD432Y-22B.
The type of Optical Sensor is TP808,it is used to detect the position of the probe
36
movement.
The size of up and down synchronous belt is 115MXL(width 6mm).
The size of left and right synchronous belt is 96 MXL(wideth 6mm).
Sample/reagent probe assembly drive sample/reagent probe to move up and down, left
and right, and make sample/reagent probe to the corresponding reagent position, sample
position, reaction cuvette position ,wash well position.
4.5 Mixer probe and drive assembly
Specification：
The type of stepping motor which control the mixer probe to be vertical movement is
17HD432Y-22B.
The type of stepping motor which control the mixer probe to be horizontal movement is
14HY115Y-22B.
The type of Detection Sensor is ST135,it is optical switch,it is used to detect vertical
position of the probe.
The type of Detection Sensor is ST135,it is optical switch,it is used to detect horizontal
position of the probe.
The size of vertical synchronous belt is 72MXL(width 6mm).
The size of horizontal synchronous belt is 60MXL(width 6mm).
It is used to stir the reaction liquid (reagents and sample) in reaction cuvette to make them
react completely.
The mixer motor type is 11H-US9.
37
4.6 Sample/reagent syringe and drive assembly
Specification：
The type of stepping motor which control the syringe to be up and down is 17HA403Y-18B.
The type of Optical Sensor is ST157,it is used to detect the up or down position of the
syringe.
Sample/reagent syringe is used to aspirate the proper amount of reagent and sample, the
amount is determined by software settings through singlechip program which controls
stepping motor .
4.7 7-steps washing unit and drive assembly
Specification：
The type of stepping motor which control the washing arm to be up and down is
17HD432Y-22B.
The size of up and down synchronous belt is 72MXL(width 6mm).
The type of Optical Sensor is TP808,it is used to detect the up or down position of the
washing arm.
Washing unit is used to wash cuvettes and keep cuvettes clean, make the reaction result
more accurate.
There are 7 wash needles on the washing unit. five short needles are used to dispense
distilled water into cuvettes,seven long needles are used to aspirate water from cuvettes.
The No.7 wash needle is installed with a silicon wiper.
38
7-steps washing unit
The No.7 wash needle is installed with a silicon wiper
4.8 Photometric System
Photometric system includes tungsten-halogen lamp、reaction cuvette、optical fiber、light
filter、signal process board and so on.
The lamp gives out the lights and the lights go into the cuvettes of the reaction tray, transmit
through the cuvettes and separated by light filters to be specific wavelengths, and then go
to the signal process board. the light signal are converted to electric signal through
photodiod .the electric signal is amplified and converted to the A/D conversion circuit, and
then sent to the main controller board,which will submit the absorbance to the PC for
calculation.
The absorbance is taken when the reaction cuvette rotates to the corresponding optical
path.
Photometric Principle figure:
39
（Tungsten-halogen Lamp） (Optical Fiber)
The most thick fiber is connected

with 340nm wavelength channel.
（The bracket of light filters）
40
Code disk
Optical Fiber
Lamp
Incubation groove
Temperature switch Temperature sensor
4.9 Temperature control process of Reaction Tray
Temperature control assembly of reaction tray includes:heating loop, temperature
switch(KSD9700),temperature sensor(LM95071),power board.
Temperature switch will cut off ,when temperature of reaction tray reaches to be more than
60 centigrade, avoiding too hot.
Temperature sensor (LM95071) is surface mounted IC,when install it,it should be done to
daub some heat transfer silicone on the induction side and donot fix so tight that damage
the chip.
The process of temperature control:
When temperature sensor(LM95071) of reaction tray transmits the temperature signal to
main controller board, The main controller board makes temperature signal compare with
temperature setted up by software. If temperature of reaction tray is lower than the

41
temperature setted up by software, then main controller board transmits signal to power
board,making LR3410(V1) breakover,DC 24V power will load to the heating loop, the
heating loop will heat the reaction groove,the D8 indicator of power board always lights. If
temperature of reaction tray reaches to the temperature setted up by software, then main
controller board transmits signal to power board,making LR3410(V1) cut off, the heating
loop stops heating, the D8 indicator of power board always blink.
+24v power
output interface
for heating loop
V1(LR3410)
D8 indicator
(Power Board)
（Heating loop）（Temperature switch. KSD9700）
42
（Temperature sensor.LM95071）
4.10 Water Temperature Control process of water tank
Water Tank temperature control Assembly include water tank,temperature
switch(KSD9700),and temperature sensor(LM95071),power board.
When the water temperature of tank is higher than 60℃,the temperature switch will be
automatically cut off in case of overheating.
The process of temperature control:
When temperature sensor(LM95071) of water tank transmits the temperature signal to
water temperature setted up by software. If water temperature is lower than the
board,making LR3410(V2) breakover,then DC 24V power is added on the calefaction
stick.it will heat water,the D10 of power board always lights. If water temperature reaches to
the temperature setted up by software, then main controller board transmits signal to power
board,making LR3410(V2) cut off, the calefaction stick stops heating, the D10 indicator of
power board always blink.
43
DC24V output interface
V2(LR3410)
D10 indicator
(Power Board)
4.11 System Power Supply
TC220 system power supply consists of one switch power supply （SP-320-24）.it provides
working DC voltages for some electric parts as belows:
DC +24V supply power for the following parts:
1.Power board
2.M7 motor subdivision board
3.Bus board
4.Water controller board
5.Main controller board
1. Bus board
2. Water controller board
3. Main controller board
4. Reagent Refrigeration Pinboard
DC -12V supply power for the following parts:

44
2. Signal process board
1. Bus board
(SP-320-24 Switch Power Supply)
(Back view of Switch Power Supply)
45
4.12 Circuit board function description
4.12.1 Main controller board function
Main controller board achieves the following functions:
⑴ Communicate with PC and execute command which is from chemistry software;
⑵ Collect signal from the signal process board；
⑶ Indicate the reaction tray rotation position;
⑷ Detect the signal of distilled water level sensor and waste level sensor;
⑸ Communicate with the Bus board ；
⑹Communicate with power board and send control command；
⑺ Communicate with water controller board and send control command；
⑻ Receive temperature signal of water tank;
⑼ Receive temperature signal of reaction tray;
46
Communication Port with

Reset switch Programming port
Signal Process Board
D10 indicator
D9 indicator
D7 indicator
D12 indicator
D6 indicator
D13 indicator
D18 indicator
Communication
D3 indicator
Port with Water
Connected with
controller board
temperature sensor
of reaction tray
Communication
Port with Power
board
Connected with
temperature sensor
Connected with level sensor
of water tank
of water and waste
D2 indicator
D1 indicator
Power inlet D4 indicator
Communication Port
with bus board RS323 Port
D5 indicator
Communication Port with bus

board
47
Indicator Meaning Description

D1indicator Main controller board send data to computer
D2 indicator Computer send data to main controller board
D3 indicator Power supply
D4 indicator Bus board send data to main controller board
D5 indicator Main controller board send data to Bus board
D6 indicator When the reaction tray stop on the initialization position,it is bright
D7 indicator When the reaction tray is rotating,it is blinking
D9 indicator Not used
D18 indicator Singlechip program is running
4.12.2 Bus board
Four motor drive boards insert into slots which are on the bus board.and the bus board
collects and send mechanical motor position signal to the main controller board.and the
main controller board send back command to bus board according software operation.
One side of bus board

four motor drive boards insert into the
four slots
48
It is connected Power inlet

It is connected
with the main
with the main
controller board
controller board
The four signal sockets connected with

motor pinboards which are related with
mechanical arm
4.12.3 Signal Process Board
Signal Process Board Function: the light signal are converted to electric signal through
photodiod .the electric signal is amplified and converted to the A/D conversion circuit, and
then sent to the main controller board,which will submit the absorbance to the PC for
calculation.When the board is added with power,the D9 and D10 indicator always light.
340nm 405nm 450nm 510nm 546nm 578nm 620nm 660nm 690nm
Communication Port
D10 D9
with Main controller
Board
Signal Gain Signal Offset
(View of the Signal Process Board) Adjustment Adjustment

Potentiometer Potentiometer
49
4.12.4 Liquid Level Detection Board
When aspirating samples/reagents and dispensing into reaction tray, the chemistry
analyzer requires the probe to dip into the liquid for a specific depth(2mm below the liquid
level), so as to avoid carryover that has impacts on test results, and to avoid air aspiration
when the reagent/sample is insufficient. Also when washing the probe in wash well, probe
also need to stay at a special depth to wash the inner side and outer side.
When the sample/reagent probe touches the sample/reagent level, there will be a change
in the capacitance. As the capacitance changes, the phase changes.The phase
discriminator then outputs a voltage variation,which will be filtered by a low-pass filter. After
that, a band-pass filter takes the variation and reshapes it to pulse signals, which are finally
transmitted to the main controller board.
Liquid level detection board
There are four groups of jumper on the liquid level detection board,sensitivity of the liquid
level detection can be adjusted through add or remove jumper cap.more jumper caps
cause more sensitive liquid level detection.
Four groups of
jumper.normally we
install two jumper caps.
50
D1
D2
When the machine is standby,the D1 is always bright.and D2 is always black.
If the sample/reagent probe touch the liquid,the D1 and D2 blink quickly.
Anti-Crash subassembly
When the probe crashs in horizontal or vertical direction,the anti-crash subassembly will be
active,and it sends signal to motor drive board and make sample/reagent probe arm stop
running.the error message will be appeared.
51
4.12.5 Power Board
Power Board function:
⑴ Control temperature of reaction tray;
When temperature sensor(LM95071) of reaction tray transmits the temperature signal to
temperature setted up by software. If temperature of reaction tray is lower than the
board,making LR3410(V1) breakover,DC 24V power will load to the heating loop, the
heating loop will heat the reaction groove,the D8 indicator of power board always lights. If
temperature of reaction tray reaches to the temperature setted up by software, then main
controller board transmits signal to power board,making LR3410(V1) cut off, the heating
loop stops heating, the D8 indicator of temperature control board always blink.
⑵ Control water temperature of water tank;
When temperature sensor(LM95071) of water tank transmits the temperature signal to
water temperature setted up by software. If water temperature is lower than the
52
board,making LR3410(V2) breakover,then DC 24V power is added on the calefaction
stick.it will heat water,the D10 of power board always lights. If water temperature reaches to
the temperature setted up by software, then main controller board transmits signal to power
board,making LR3410(V2) cut off, the calefaction stick stops heating, the D10 indicator of
power board always blink.
4.12.6 Water Controller Board
This board is connected with valve,pump and control their working condition according
command which is from main controller board.
Water dispensing valve

Pressure sensor.it is
connected with water
Outer probe washing valve
tank
Two water pumps
Communication port
Inner probe washing valve
with main controller
board
Backwater peristaltic pump
Drain pump
Liquid level sensor of water tank
Air valve
53
4.12.7 Reagent Refrigeration Pinboard
Reagent refrigeration pinboard connects with two refrigeration semiconductor and
temperature sensor of reagent tray.
DC12V output for one refrigeration semiconductor
DC12V power input from switch power supply
DC12V output for one refrigeration semiconductor
Cooling fan of
refrigeration
semiconductor
Communication port
with power board
Connected with
temperature sensor
of reagent tray
Connected with
Connected with temperature switch
temperature switch
Cooling fan of refrigeration semiconductor
Control reagent temperature process:
When temperature sensor(LM95071) of reagent tray transmits the temperature signal to
temperature setted up by software. If temperature of reagent tray is higher than the
board,making LR3410(V1) breakover,DC 12V power will load to the refrigeration

54
semiconductor, the D1 and D2 indicator of refrigeration pinboard always lights. If
temperature of reagent tray is lower than the temperature setted up by software, then main
controller board transmits signal to refrigeration pinboard ,making LR3410(V1) cut off, the
D1 and D2 indicator of temperature control board is off.
4.12.8 M7 Motor Subdivision Board
This board is used to make sample/reagent syringe stepping motor movement
accurate.
Program switch
Program switch Subdivision Motor Style
1 2 Number
ON OFF 16 8
55
MotorStyle is 8
Note:if there is M7 motor subdivision board installed,the motorstyle value should be 8
in the registry table of computer.
otherwise the sample/reagent syringe movement will be abnormal.
4.12.9 Stepping Motor Drive Board
This board is used to drive the corresponding stepping motor according command from
main controller board.there are four pieces motor drive boards,see the following
pictures.
56
This board drives two stepping motors,one is mixer probe

vertical movement stepping motor,the other is mixer probe
horizontal movement stepping motor
This board drives two stepping motors,one is

sample/reagent syringe stepping motor,the
other is 7-steps washing unit stepping motor
This board drives two stepping motors,one is

sample/reagent probe vertical movement stepping
motor,the other is sample/reagent probe horizontal
movement stepping motor
This board drives one stepping motors which

control rotation of reaction tray
Four stepping motor drive boards have own program switch position except the board
which drives the rotation stepping motor of reaction tray.
When you replace motor drive board,you should notice the program switch
position.otherwise the corresponding motor movement will be abnormal.
Program Switch Postion Figure:
K2-5:off.K2-4:on.K2-3:on.K2-2:off.K2-1:off K1-5:off.K1-4:on.K1-3:off.K1-2:on.K1-1:on
K2-5:off.K2-4:off.K2-3:on.K2-2:on.K2-1:on K1-5:off.K1-4:off.K1-3:off.K1-2:on.K1-1:off
K2-5:off.K2-4:off.K2-3:on.K2-2:offK2-1:on K1-5:off.K1-4:off.K1-3:on.K1-2:on.K1-1:off
Program Switch Position Description
57
When the stepping motor movement problem is occurred,the error message will
appear and give “Motor Number is error”.in the following table,it is shown that the
motor number is related with motor function.
Stepping Motor Number Function
Motor 0 Drive reaction tray rotation
Motor 2 Drive 7-steps washing unit to be up or down
Motor 5 Drive sample/reagent arm to be up or down
Motor 6 Drive sample/reagent arm to be left or right
Motor 7 Drive sample/reagent syringe to be up or down
Motor 11 Drive mixer arm to be up or down
Motor 12 Drive mixer arm to be left or right
4.12.10 Mixer Motor Power Board
DC+2.4~2.5V output
DC+12V input
Adjust this potentiometer to make output

voltage to be 2.4~2.5V,and mixer probe
will rotate 3000~4000RPM
Mixer motor power board
58
Chapter 5 Component Adjustment and Replacement
5.1 Signal process board voltage adjustment
Steps:
1. Offset Voltage adjustment of the signal process board.
Remove the power connector of Tungsten-Halogen Lamp and switch on the machine,then
set the multimeter to DC 200mV grade. The black pen of the multimeter is connected to
ground point, and the red pen measure the corresponding test points of wavelength, adjust
the corresponding potentiometer of offset voltage adjustment to ensure the voltage to be
5～10mv.
according this adjustment procedure,to adjust the offset voltage of all the wavelengths.
2. Gain voltage adjustment of the signal process board
Firstly initialize the machine,and add 400ul distilled water into the NO.11 reaction
cuvette,waiting thirty minutes, set the multimeter to DC 20V grade. The black pen of the
multimeter is connected to ground point, and the red pen measure the corresponding test
point of wavelength, adjust the corresponding potentiometer of gain voltage adjustment to
ensure the voltage to be 3.7～3.8V.
according this adjustment procedure,to adjust the gain voltage of all the wavelengths.
59
340nm 405nm 450nm 510nm 546nm 578nm 620nm 660nm 690nm
Not used
Voltage Test Point D10

D9
Gain Potentiometer Offset Potentiometer
5.2 Reaction tray position adjustment
After initialize the machine,the light of lamp should focus the middle of the NO.11 cuvette.if
the reaction tray position is not suitable,it is necessary to adjust it as following steps.
1.Initialize the machine and remove the cap of reaction tray.
The cap of reaction tray
60
2.Loosen the three retaining screws.
Three retaining
screws
3.Rotate the reaction tray and make light of lamp focus the middle of the NO.11 cuvette.
light of lamp focus the middle of the

NO.11 cuvette
4.Tighten the three screws and initialize the machine to confirm.
5.3 Tungsten-Halogen Lamp replacement
Steps
1.Switch off the machine.
2.Remove the cover which is shown as following picture
Unscrew the two screws and

remove the cover
61
3.Unplug the power connector of lamp
The connector of
lamp
4.Loosen the retaining screw of lamp and then take out the lamp.
The retaining screw
5.Put the new lamp into lamp hole and connect the power connector of lamp.
6.Switch on the machine and then initialize.
7.Remove the cuvettes group which is from NO.11 to NO.20 .
the cuvettes group

which is from NO.11
to NO.20
8.Adjust the lamp position and observe whether the light focus the middle of incubation
groove hole or not.if it is,tighten the retaining screw of lamp and reinstall the cover of lamp.
9.Finish replacement.
62
Note:after you replace the new lamp,you should adjust signal process board according 5.1
signal process board voltage adjustment.
5.4 Sample/reagent probe position adjustment
When login the operating software,click “maintenance” and select “Instrument
settings”.then input password”999” then enter this menu which is shown in the following
picture.
there are three setup columes in this menu.
CRU setup colume and time setup colume are only filled in with factory value.
The arm setup colume is used to adjust sample/reagent probe position which is related with
wash well,sample position ,reagent position and cuvette position.
Note:before adjust sample/reagent probe ,it should be done to perform initialization.
63
5.4.1 Wash well position for sample/reagent probe
1.Click “Wash Pos.”button,and then click “Start”button,the probe tip should be upon in the
middle of wash well.if not,adjust two retaining screws as following pictures.
After click it,it

show red color
The other retaining screw
One retaining screw
2.Click “Deep” button ,then the probe will insert into wash well.
3.Increase or decrease the depth value to make the probe tip just touch the mouth of water
injecting hole which is in the wash well.
Depth value of wash well
64
4.Use the depth value which is got from step 3 and then fill in final depth value.
5. Click “Arm initialization”button,and save,then finish the adjustment.
5.4.2 Cuvette position for sample/reagent probe
1. Click “Cuvette”button,and then click “Start”button,the probe rotates to the reaction
tray.and the probe tip should be upon in the middle of NO.1 cuvette.if not,increase or
decrease the step value.
step value of cuvette
2. Click “Deep” button ,then the probe will insert into the NO.1 cuvette.
3. Increase or decrease the depth value to make the probe tip just touch the bottom of the
NO.1 cuvette.
Depth value of cuvette
4. Use the depth value which is got from step 3 to subtract 2,and then fill in final depth
value.
65
5.4.3 Sample position for sample/reagent probe
1. Put one empty serum cup on the NO.1 sample position,and the other empty serum cup is
on the NO.18 sample position.
2. Click “1#Sample”button,and then click “Start”button,the probe rotates to the sample
tray.and the probe tip should be upon in the middle mouth of NO.1 serum cup.if
not,increase or decrease the step value.
Step value of NO.1 sample position
3.Click “Arm initialization”button.
4. Click “18#Sample”button,and then click “Start”button,the probe rotates to the sample
tray.and the probe tip should be upon in the middle mouth of NO.18 serum cup.if
Step value of NO.18 sample position
5. Click “Deep” button ,then the probe will insert into the NO.18 serum cup.
NO.18 serum cup.

66
The depth value of

serum cup
value.
Note:our software allow customer to add different tube in the “data dictionary menu”.when
customer add new tube,service engineer should adjust depth value for the new tube
according step 6.and notice when they load new sample in the “edit” menu,then should
select correct “cup type” for the sample.otherwise some wrong result will be occurred.
5.4.4 Reagent position for sample/reagent probe
1. Put one empty reagent bottle on the NO.1 reagent position,and the other empty reagent
bottle is on the NO.26 reagent position.
2. Click “1#Reagent”button,and then click “Start”button,the probe rotates to the reagent
tray.and the probe tip should be upon in the middle mouth of NO.1 reagent bottle.if
67
Step value of NO.1

reagent bottle
3. Click “Arm initialization”button.
4. Click “26#Reagent”button,and then click “Start”button,the probe rotates to the reagent
tray.and the probe tip should be upon in the middle mouth of NO.26 reagent bottle.if
Step value of NO.26

reagent bottle
5. Click “Deep” button ,then the probe will insert into the NO.26 reagent bottle.
NO.26 reagent bottle.
Depth value of reagent bottle
68
value.
5.5 Sample/reagent probe replacement
If the probe is bent or damaged, it must be replaced immediately. follow the procedure
given below to replace the damaged or bent probe.
1. Switch off the machine.Remove the cap of sample/reagent probe arm.
the cap of sample/reagent probe arm
2. Take out the sample tray,and then pull the probe arm to its highest point. rotate the probe
arm to move the probe to a position above the sample tray and convenient to operate.
3. Use a small screwdriver to loosen the two retaining screws on the probe.
One screw
The other screw
4. Carefully unplug the aspirator tube connector.
69
the aspirator tube connector
5. Slowly pull the probe away from the probe arm.
WARNING:
Store the removed probe in a safe place where it will neither

endanger people working around the area nor be damaged.
NOTE:
Exercise caution when pulling the probe away from the arm.
7. Install the new probe according reverse steps.
BIOHAZARD:
Dispose of the bent or damaged probe in accordance with your

local or national guidelines for biohazard waste disposal.
5.6 Mixer probe position adjustment
When login the operating software,click “maintenance” and select “Instrument
settings”.then input password”999” then enter this menu which is shown in the following
picture.
The stiring setup colume is used to adjust mixer probe position which is related with wash
well,cuvette position.
Note:before adjust mixer probe position,it should be done to perform initialization.
70
5.6.1 Wash well position for mixer probe
1.Click “Wash Pos.”button,and then click “Start”button,the probe tip should be upon in the
middle of wash well.if not,adjust the retaining screw as following pictures.
Mixer motor
Retaining screw
Wash well of mixer probe
2.Click “Deep” button ,then the probe will insert into wash well.
3.Increase or decrease the depth value to make the probe tip just touch the mouth of water
injecting hole which is in the wash well.

71
Depth value of wash well
Wash well
Mixer probe
the mouth of water injecting hole
4.Use the depth value which is got from step 3 and then fill in final depth value.
5. Click save and exit the menu then initialize the machine.
6. If the position is not good ,adjust it again.
5.6.2 Cuvette position for mixer probe
1. Click “Cuvette”button,and then click “Start”button,the probe rotates to the reaction
tray.and the probe tip should be upon in the middle of NO.1 cuvette.if not,increase or
decrease the step value.
Step value of cuvette
72
2. Click “Stiring deep” button ,then the probe will insert into the NO.1 cuvette.
NO.1 cuvette.
depth value of cuvette
value.
5. Click save and exit the menu then initialize the machine.
5.7 Mixer probe replacement
If the probe is bent or damaged, it must be replaced immediately. follow the procedure
given below to replace the damaged or bent probe.
1. Switch off the machine.Remove the cap of mixer probe arm.
The cap of mixer probe arm
2. Unplug the power connector of mixer motor.
73
The power connector of mixer
3. Remove the retaining screw of mixer probe arm and then take out the mixer probe arm.
The retaining screw of mixer probe arm
4. Pull out the mixer probe carefully.
5. Install the new probe according reverse steps.
74
5.8 Synchronous belt replacement
There are synchronous belts for the left/right and up/down of the sample/reagent probe arm
and 7-steps washing unit. It also exists for the reaction tray.
Function Belt Size
Sample/reagent probe arm up/down 115MXL(width 6mm).
Sample/reagent probe arm left/right 96MXL(width 6mm).
7-steps washing unit up/down 72MXL(width 6mm).
Reaction tray rotation 119MXL(width 6mm).
Mixer probe arm up/down 72MXL(width 6mm).
Mixer probe arm left/right 60MXL(width 6mm).
5.9 Light filter replacement
Steps:
1. Switch off the machine.
2. Remove the cover which is shown as following picture
Unscrew the two screws and

remove the cover
3. Unscrew the nine screws which fix the cover of the machine ,and then carefully lift and
remove the whole cover of the machine.

75
4. Unscrew the three retaining screws which fix the shield cover of signal process board.
The shield cover of signal

process board
5. Unplug the signal connector of signal process board from main controller board.
6. Unscrew the two retaining screws of signal process board.
7. Carefully remove the signal process board and put it on the dry and clean place.
76
Light filter bracket
8. For example we replace the 690nm wavelength filter.
9. Loosen the retaining screw of 690nm wavelength locating sleeve and carefully take out
the fiber of 690nm.
Fiber of 690nm
10. Push out the locating sleeve and take out the whole locating sleeve.
77
11. Open the locating sleeve and push out the filter using tool.
Note:during push out filter,donot touch the surface of filter.
Light filter
12. Install the new light filter according the following direction.
78
Locating sleeve of filter Locating sleeve of fiber
Filter
13. Install the whole locating sleeve on the filter bracket.and tighten the retaining screw.
Note:during tighten the retaining screw,donot screw too tight,otherwise filter will be
damaged by fracturing.
during tighten the retaining screw,donot screw too

tight,otherwise filter will be damaged by fracturing.
14. Adjust the signal process board according 5.1 signal process board voltage adjustment.
15. Finish the adjustment.
79
Chapter 6 Maintenance
6.1 Daily Maintenance
It is necessary to perform daily maintenance procedure to ensure reliable instrument
performance.
6.1.1 Check the distilled water volume of water bucket
1. Verify the machine is not testing.
2. Check the level of distilled water. If fluid level is low, replace it as follows.
① Loosen off the cap on the distilled water bucket.
② Pour some newly-made distilled water in the bucket.
③ Fasten the cap on the distilled water bucket.
3. Check the tubing of distilled water whether there is some leakage or oppressed tubing or
not.
6.1.2 Check waste solution
1. Verify the machine is not testing.
2. Check the level of waste solution. If the level is low, this procedure needn’t to be
executed. If the waste solution is full, proceed to next step.
NOTE: It is recommended that you should execute this procedure every day.
① Loosen offf the cap on the waste solution bucket.
② Empty the waste solution according to local legislation.
③ Place the cap on the waste bottle, and fasten it properly.
80
6.1.3 Check the signal value of all cuvettes
1. Enter the maintenance menu,and click “Ready to test” button.
Ready to test
2. The machine automatically tests the signal value of all cuvettes.
3. In this menu,we set the minimum signal value is 25000 and maximum signal value is
65535.if the signal value of cuvette is less than 25000 or more than 65535.the software will
mark red color on the corresponding cuvette number.the best signal value range of cuvette
is from 35000 to 55000.
When you find the cuvette which is bad signal value.you should take out the cuvette,and
observe what’s wrong with it.and clean it using wash solution or replace it.
6.1.4 Check sample/reagent probe clogging
When the probe is clogged, the fluid flow will become abnormal and the sample/reagent
volume which is aspirated is unstable.
81
1. Initialize the machine.
2. Enter maintenance menu and click “Motion detection”
3. Click “internal probe wash” button,and then click “Start” button.immediately observe the
probe tip.
4. Normally very thin liquid flows out from probe.and the liquid column is straight.otherwise
the probe is clogged.you can use a needle to unclog the probe from tip.
the liquid column is

straight
6.2 Weekly Maintenance
WARNING:
The probe tip is sharp and can cause puncture wounds. To prevent injury, exercise caution when working around the
probe.
82
BIOHAZARD:
Wear gloves and lab coat and, if necessary, goggles.Dispose of the used gauze in accordance with your local or national
guidelines for biohazard waste disposal.Dispose of the waste in accordance with your local or national guidelines for
biohazard waste disposal and consult the manufacturer or distributor of the reagents for details.
6.2.1 Clean sample/reagent probe and mixer probe
2. Take out the sample/reagent track from tray.
3. Pull the sample/reagent and mixer probe arm to the highest point by hand. Rotate the
probe arms to move it to a position above the convenient place to operate.
4. Pinch acid or alkaline detergent-soaked gauze with tweezers and gently clean the
exterior of probes.
5. Pinch deionized water-soaked gauze to clean probes.
6. After cleaning, gently pull the probe arms to its highest point and rotate the probe arms to
move the probes to a position above the corresponding wash well.
7. Switch on the machine and perform initialization.
6.2.2 Clean two wash well
2. Pull the sample/reagent probe and mixer arm to its highest point. Rotate the arms to
move away from the wash well.
3. Clean the inside of and the place around wash well with cotton swabs.
4. Pull the sample/reagent probe and mixer arm to its highest point and rotate them to be
83
above corresponding wash well.
5. Switch on the machine and perform initialization.
6.2.3 Clean sample and reagent tray
2. Take out reagent bottle and sample cup from sample or reagent tray.
3. Wash sample and reagent tray with clean water and wipe it dry with clean gauze.
4. Wash sample and reagent rack with clean water and wipe it dry with clean gauze.
5. Load the sample and reagent rack.
6.2.4 Clean the reaction tray
Using gauze moistened with distilled water, wipe the surface and around of the reaction
tray.
NOTE: DO NOT clean the reaction tray using gauze moistened with ethanol or detergent
which may cause the permanent damage to cuvettes.
6.2.5 Clean 7-steps washing needles
Clean the individual nozzles with gauze moistened with distilled water from up to down.
NOTE: DO NOT touch the surface with tweezers directly preventing from scratch of
nozzles.
6.2.6 Clean cuvettes using wash solution
Normally the machine clean cuvettes using distilled water during testing.so the customer
should perform the cleaning at least one time per week.

84
1. initialize the machine.
2. Fill one empty reagent bottle with wash solution.
Note:wash solution should be bought from TECOM corporation with machine’s order.
3. Put the wash solution bottle on the one empty reagent position.
4. Enter “maintenance”menu,and select “Cuvettes cleaning” menu.
5. Input the current wash solution botttle position.
current wash solution botttle position
6. Click the washing button and then the machine automatically aspirate and dispense
200ul wash solution into every cuvette.
Washing button
7.This procedure makes the cuvettes soak with wash solution soak in and clean it.
6.2.7 Check the signal value of all cuvettes.
1. Initialize the machine.
2. Enter “maintenance”menu,and select “cuvette checking”.
85
3.Click “Add water”button,and the machine automatically add some water into every
cuvette.
4. after the process of add water,click”Check cuvette” then the machine automatically
detect signal value of every cuvette.
5. if the signal value of cuvette is less than 25000 or more than 65535.the software will
mark red color on the corresponding cuvette number.the best signal value range of cuvette
is from 35000 to 55000.
When you find the cuvette which is bad signal value.you should take out the cuvette,and
observe what’s wrong with it.and clean it using wash solution or replace it.
6. Click “remove water” and the machine empty water from all cuvettes.
6.3 Monthly Maintenance
6.3.1 Clean incubation groove of reaction tray
2. Remove all cuvettes holder from reaction tray and put it on a clear table.
3. Wipe the incubation groove using gauze.
NOTE: DO NOT scratch the light path when wipe the incubator.
86
Incubation groove
4. Place the cuvette holders back into reaction tray.
5. Perform checking the signal value of all cuvettes.
6.3.2 Clean the panel of machine
2. Wipe the panel of the machine with clean gauze (water or disinfector-dipped gauze if
necessary).
6.3.3 Clean distilled water bucket
2. Unscrew the cap of distilled water bucket(together with the distilled water tube and the
sensor).
CAUTION:
After removing the cap (together with the tube and sensor), place it on a clean table.
3. Wash the bucket interior with deionized water. Use a clean brush to clean the interior if
necessary.
4. Wash the water tube and the sensor with deionized water. Use clean gauze to wash
them if necessary.
87
5. Wipe water off the bucket exterior, water tube and sensor cable with clean gauze.
6. Add fresh distilled water into water bucket.
7. Screw the cap (together with the tube and sensor) back onto the bucket until secure.
6.3.4 Clean waste solution bucket
BIOHAZARD:
Exercise caution and do not spill the waste onto other people or things.
2. Unscrew the cap (together with the waste tube and the sensor).
BIOHAZARD:
After removing the cap of the waste tank (together with the waste tube and sensor), place it on an appropriate place to
avoid biohazard contamination.
3. Empty waste solution bucket.
4. Wash the bucket interior with clean water. Soak the bucket with disinfector if necessary.
5. Wash the waste tube and the sensor with clean water.
6. Wipe water off the bucket exterior, waste tube and sensor cable with clean gauze.
7. Screw the cap (together with the waste tube and sensor) back onto the bucket until
secure.
CAUTION:
Ensure the waste tube is over the tank and not blocked, bent, or twisted. A blocked, bent or twisted waste tube may lead
to wastewater overflow that may damage the analyzer.
88
6.3.5 Clean water filter
The water filter is connected with outlet of water tank.it is used to filtrate some dirty material
from water tank.it is necessary to clean it monthly.otherwise it will be clogged and affect
water flow.
Water filter
Water filter
Filter screen
6.3.6 Clean dust screen
2. There is one dust screen on the rear of the machine.

89
Dust screen
3. Take it out by hand
4. Wash the screen with clean water and dry it by air-drying.
5. Install the screen back to the rear of machine.
90
Appendix A Spare Parts Price List
System P/N Description Qty Price

PCB001 Main Controller Board ea. 420.00
PCB002 Signal Process Board ea. 280.00
PCB003 Universal Stepping Motor Drive Board ea 93.00
PCB004 Reaction Tray Stepping Motor Drvie Board ea 65.00
PCB005 Sample/reagent Syring Stepping Motor Drive Board ea 93.00
PCB006 Bus Board ea 20.00
PCB PCB007 Liquid Level Detection Board ea 53.00
PCB008 Motor Pinboard ea 12.00
PCB009 Water Controller Board ea 45.00
PCB010 Reagent Refrigeration Pinboard ea 28.00
PCB011 M7 Motor Subdivision Board ea 69.00
PCB012 Mixer Motor Power Board ea 6.00
PCB013 Power Board ea 160.00
PS001 Switch Power Supply ea 135.00
Power PS002 Power Transformer(24v-12v,10A) ea 10.00
Supply PS003 Power Transformer(24v-5v,10A) ea 12.00
PS004 Power Transformer(24v-12v,5A) ea 8.00
Photometer PR001 Optical Fiber ea 520.00
PR002 Tungsten-Halogen Lamp ea 40.00
PR003 Tungsten-Halogen Lamp with Holder ea 50.00
PR004 Light Focus Subassembly ea 120.00
PR005 340nm Light Filter ea 89.00
91

PR014 Photodiode of Signal Process Board ea 7.00
Valve V001 Two-Way Valve ea 130.00
PP001 Peristaltic Pump Complete ea 90.00
PP002 Peristaltic Pump Cassette with Tube ea 9.00
PP003 Peristaltic Stepping Motor ea 75.00
PP004 Water Pump 1 ea 110.00

Pump
PP005 Peristaltic Pump Tube ea 6.00
PP006 Drain Pump1 ea 280.00
PP007 Drain Pump2(Nidec DC24V) ea 58.00
PP008 Water Pump 2(Nidec Pump DC12V) ea 45.00
SE001 Sample/Reagent Syringe Cavity without Motor ea 40.00
SE002 Sample/Reagent Syringe Piston O-ring ea 1.50
SE003 Sample/Reagent Syringe Nozzle O-ring ea 1.00
Syringe SE004 Sample/Reagent Syringe Piston ea 15.00
SE005 Sample/reagent Syringe Guide Sleeve ea 3.00
SE006 Retaining Nest ea 1.00
SE007 Connection Sleeve between Piston with Gear Rack ea 2.00
Motor M001 23HY109-20B2 Stepping Motor ea 79.00
92

M002 17HD432Y-22B Stepping Motor ea 76.00
M003 17HA403Y-18B Stepping Motor ea 73.00
M004 Complete Sample/Reagent Probe Arm set 220.00
M005 Complete Mixer Probe Arm set 208.00
M006 Complete Washing Arm set 180.00
M007 Mixer Motor ea 10.00
MG001 Motor Gear of Sample/reagent Syringe ea 5.00
MG002 Motor Gear of Reaction Tray ea 7.00
Motor Gear of Sample/reagent Probe Horizontal

MG003 ea 6.00
Movement
Motor Gear of Sample/reagent Probe Vertical
Motor Gear MG004 ea 8.00
Movement
MG005 Motor Gear of Mixer Probe Horizontal Movement ea 6.50
MG006 Motor Gear of Mixer Probe Vertical Movement ea 8.00
MG007 Motor Gear of 7-Steps Washing Unit ea 8.00
CF001 Cooling Fan of Lamp(JDH7530B DC12V 0.5A) ea 6.00
Cooling Fan of Machine Body(HDB0924EA

Cooling Fan CF002 ea 8.00
DC24V 0.25A)
Cooling Fan of Boards Box(HDB0612MD DC12V
CF003 ea 5.00
0.12A)
F001 60MXL(width 6mm) ea 2.00
Feed Belt F003 115MXL(width 6mm) ea 2.00
F005 B120MXL(width 6mm) ea 2.00
Probe PE001 Sample/Reagent Probe with Liquid Detection Wire ea 36.00
PE002 Single Long Washing Needle ea 5.00
PE003 Long& Short Washing Needle ea 6.00
PE004 Complete Washing Needles with Holder ea 60.00
93

PE005 Mixer Probe ea 8.00
RS001 Refrigeration Semiconductor ea 25.00
RS002 Cooling Fan of Semiconductor ea 9.00
Refrigeration RS003 Cooling Water Bottle ea 8.00
System RS004 Cooling Water Cycle Pump ea 78.00
RS005 Condensator ea 60.00
RS006 Water Cooling Subassembly ea 150.00
OS001 Optical Sensor of Sample/reagent Syringe (ST157) ea 1.00
Optical Sensor of Sample/reagent Probe Vertical

OS002 ea 1.00
Movement (TP808)
Optical Sensor of Sample/reagent Probe
OS003 ea 1.00
Optical Horizontal Movement (TP808)
OS004 Optical Sensor of Washing Unit (TP808) ea 1.00
Sensor Optical Sensor of Mixer Probe Vertical Movement
OS005 ea 1.00
(TP808)
Optical Sensor of Mixer Probe Horizontal
OS006 ea 1.00
Movement (ST135)
OS007 Optical Sensor of Reaction Tray(ST151) ea 2.00
T001 Silicon Tubeφ1.5×φ3.2 m 0.50
T002 Silicon Tubeφ3×φ6 m 1.00
Tube T004 Silicon Tubeφ2×φ4 m 1.00
T006 Silicon Tubeφ1.2×φ3 m 0.50
T007 Sample/reagent Aspirator Teflon Tubeφ0.8×φ1.6 ea 10.00
Others OR001 Reaction Cuvette ea 1.00
OR002 Serum Cup(pack of 1000) ea 26.00
OR003 White Reagent Bottle ea 1.50
OR004 Yellow Reagent Bottle ea 2.00
94

OR005 Reaction Cuvette Holder ea 5.00
OR006 Direct Serial(RS232) Communication Cable ea 3.00
OR007 USB To Serial Port Convertor Cable ea 10.00
OR008 Complete Water Tank Subassembly ea 62.00
OR009 Water Tank Base Subassembly ea 40.00
OR010 O-ring of Water Tank ea 2.50
OR011 Water Tank Glass Cover Subassembly ea 20.00
OR012 Separatory Liquid Block ea 6.00
OR013 Water Filter of Water Tank Outlet ea 8.00
OR014 Water Filter Screen ea 2.00
OR015 O-ring of Water Filter ea 1.00
OR016 Temperature Sensor of Reaction Tray ea 6.00
OR017 Temperature Sensor of Reagent Tray ea 6.00
OR018 Temperature Sensor of Water Tank ea 6.00
OR019 Probe Crash Protection Subassembly ea 28.00
OR020 Incubation Groove ea 80.00
OR021 Water Container Sensor Subassembly ea 40.00
OR022 Waste Container Sensor Subassembly ea 35.00
OR023 Heating Loop ea 32.00
Temperature Protective Switch（KSD9700 250V

OR024 ea 5.00
10A 60℃）
OR025 Heating Loop with Temperature Protective Switch ea 39.00
OR026 Dust Screen ea 3.00
OR027 Water Calefaction Stick ea 10.00
Water Calefaction Stick with Temperature

OR028 ea 16.00
Protective Switch
OR029 Silicon Washing Wiper ea 0.50
95

OR030 Sample Tray Track ea 40.00
OR031 Reagent Tray Track ea 45.00
OR032 T/Y Connector ea 1.00
OR033 Water Container(10L) ea 8.00
OR034 Waste Container(10L) ea 8.00
OR035 Washing Needle Spring ea 0.50
OR036 Communication Chip of Mainboard ea 4.00
OR037 Right Hinge of Upper Cover set 3.00
OR038 Left Hinge of Upper Cover set 3.00

Temperature Protective Switch（TB02-KABD 2A 60
OR039 ea 5.00
℃）
OR040 Sample/reagent Probe Arm Cover ea 20.00
OR041 Mixture Probe Arm Cover ea 15.00
96
Appendix B Preventive Maintenance Kit

No. PN Part Description RRP Qty
1 PR003 Tungsten-Halogen Lamp with Holder 6 Months 2PCS
2 SE002 Sample/Reagent Syringe Piston O-ring 6 Months 1PCS
3 SE003 Sample/Reagent Syringe Nozzle O-ring 6 Months 1PCS
4 SE005 Sample/reagent Syringe Guide Sleeve 6 Months 1PCS
5 SE004 Sample/Reagent Syringe Piston 2 Years 1PCS
6 F001 60MXL(width 6mm) 2 Years 1PCS
10 F005 B120MXL(width 6mm) 2 Years 1PCS
11 PE001 Sample/Reagent Probe with Liquid 1 Year 1PCS
Detection Wire
12 PE005 Mixer Probe 1 Year 1PCS
13 RS001 Refrigeration Semiconductor 2 Years 2PCS
14 T007 Sample/reagent Aspirator Teflon Tube 1 Year 1PCS
15 OR001 Reaction Cuvette 1 Year 60 PCS
16 OR013 Water Filter of Water Tank Outlet 6 Months 1PCS
17 OR029 Silicon Washing Wiper 3 Months 1PCS
18 MG001 Motor Gear of Sample/reagent Syringe 1 Year 1PCS
Total Price: 308 USD
Note:
1. RRP is replacement requirement period.
2. Contents of this list is available for one machine.
97
Appendix C Error Code Message
98
Appendix D Diagram
Figure D-1 Machine’s Cover
99
Figure D-2 Code Disk of Reaction Tray
100
Figure D-3 Cuvettes
101
Figure D-4 Heating Loop with Temperature Switch
102
Figure D-5 Incubation Groove of Reaction Tray
103
Figure D-6 Lamp with Holder
104
Figure D-7 Level Sensitivity Board Connection
105
Figure D-8 Level Sensitivity Board
106
Figure D-9 Light Filters Subassembly
107
Figure D-10 Light Filters
108
Figure D-11 Light Focus Lens
109
Figure D-12 Light Focus Subassembly
110
Figure D-13 Light Focus Subassembly 02
111
Figure D-14 Sample/reagent probe Arm
112
Figure D-15 Reaction Tray Pinboard
113
Figure D-16 Reagent Cooling Subassembly
114
Figure D-17 Sample/reagent Tray with Cooling Subassembly
115
Figure D-18 Reagent Tray
116
Figure D-19 Refrigeration Semiconductor
117
Figure D-20 Sample/reagent Probe
118
Figure D-21 Sample/reagent Tray
119
Figure D-22 Separatory Liquid Block
120
Figure D-23 Single Washing Needle
121
Figure D-24 Syringe Gear Rack
122
Figure D-25 Syringe Piston
123
Figure D-26 Syringe Subassembly
TC220 Chemistry Analyzer Technology Manual(Ver1.15) 124

Figure D-27 Temperature Protective Switch

Figure D-28 Temperature Protective Switch with Sensor

Figure D-29 Washing Needle Unit

Figure D-30 Double Washing Needle

Figure D-31 Water Tank Bottom

Figure D-32 Water Tank Liquid Level Sensor

Figure D-33 Water Tank

Figure D-34 Fluidic Diagram 01





Figure D-39 Cover’s Hinge

Figure D-40 Mainboard and Bus Board Connection

Figure D-41 Mixer Probe Subassembly Connection

Figure D-42 Power Board and Mainboard Connection

Figure D-43 Power Board and Water Controller Board Connection

Figure D-44 Power Board and Water Tank Connection

Figure D-45 Power Socket Connection 01

Figure D-46 Power Socket Connection 02

Figure D-47 Refrigeration Pinboard Connection

Figure D-48 Stepping Motor Drive Boards

Figure D-49 Water Controller Board Connection

TC220 Manual Tenico V1.15

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TC220 Chemistry Analyzer Technician Manual(Ver1.

555# Gao Xin Ave., Nan Chang National

Hi-tech Industry Development Zone, Nan Chang,

Jiang Xi 330096, P. R. China

Chapter 1 Analyzer Introduction

1.1 System Structure

TC220 biochemistry analyzer is composed of analyzing module and operation unit.

1.1.1Appearance pic of Analyzer

Sensor and Fluidic connector interface

Cooling and power switch

Female 9-pins serial port Power inlet

Earth line connector Fuse

（Planform view closing analyzer’s upper cover）

7-steps wash unit

Wash well of sample/reagent probe

（Planform view after opening analyzer’s upper cover）

1.1.2 Composition of Analyzer

assembly、temperature control system、optics system etc.

1.1.2.1 Sample/Reagent Tray Assembly

（Sample/reagent tray assmebly pic）

● Function and Composition

Sample/reagent tray assembly including sample and reagent tray 、 sample/reagent

be dispensed to the correct cuvette position which is controlled by software.

Sample tray has 18 sample positions

● Sample cup is one-off consumables,cann’t reuse ,otherwise it will be appear carryover.

Sample/Reagent Dispenser System

Probe rotor Sample/reagent probe

（Sample/reagent probe mechanical assembly pic）

● Function and Composition

Sample/reagent dispenser system is consist of sample/reagent probe、 rotor mechanical

assembly、reagent/sample syringe assembly,carry sample or reagent to reaction cuvette.

Sample volume range：1～50ul.

Reagent volume range：10～400ul.

which is sample/reagent tray→reaction tray→wash well and finishs adding sample or

reagent into the reaction cuvette.

suitable for different liquid level height sample or reagent.

direction,the system will alarm.

arm moves. Otherwise, it may lead to personnel injury or equipment damage.

1.1.2.2 Reation Tray Assembly and Temperature Control System

Tray lock knob

(Reaction tray assembly pic)

（Reaction cuvette holder pic）

● Composition and Function

place,meanwhile,it is as colorimetric measurement optics device .When reaction cup pass

through the optics route,the analyzer measures absorbance of reaction liquid.

Temperature control is used to offer a homothermal environment for reaction.

Reaction tray：using 6 reaction cuvette holders to load total 60 reaction cuvettes，each

holder loads 10 reaction cuvettes.

1.1.2.3 Photometric System

Photometric system includes tungsten-halogen lamp、reaction cuvette(colorimetric cup)、

9 wavelengths, which are：340nm、405nm、450nm、510nm、546nm、578nm、620nm、

proper site for installation.

your local distributor.

Service Department or your local distributor immediately.

2.2 Installation Environment Requirements

it will not perform as promised.

2.2.1 Installation Environment Requirements

⊙ The system is for indoor use only.

⊙ The installation site should be well ventilated.

⊙ The installation site should be free of dust as much as possible.

⊙ The installation site should not be in direct sun.

⊙ The site should not be near a heat or draft source.

⊙ The bearing platform (or ground) should be free of vibration.