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Proceedings of the 34th World Small

Animal Veterinary Congress
WSAVA 2009
São Paulo, Brazil - 2009

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Reprinted in IVIS with the permission of the Congress Organizers

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Abstracts - oral presentation

A RESEARCH ON THE EFFECTS OF LEUKOREDUCTION ON SOME IMMUNOLOGICAL

PARAMETERS AND TRANSFUSION REACTIONS AND THE
DEA 1.1 PREVALENCE OF KANGAL DOGS - 549
Özcan M 1; Inal Gültekin G 1; Matur E 1; Ergül Ekiz E 1; Deniz G 2; Eraslan Uygur E 1; Kayar A 3.
1. Istanbul University Veterinary Faculty, Department of Physiology Avcılar- Istanbul, Turkey;
2. Istanbul University Institute of Experimental Medicine, Department of Department of Immunology Çapa-Istanbul, Turkey;
3. Istanbul University Veterinary Faculty, Department of Internal Medicine Avcılar- Istanbul, Turkey.
This work was supported by Research Fund of the Istanbul University. Project number: 301/05012005.
E-mail: guldalinal@yahoo.com

Passenger leukocytes (WBC) might have potential adverse effects in
recipient dogs particularly in cases of repeated BTs. The aim of this
study was to determine the dog erythrocyte antigen (DEA) 1.1 blood
type frequency for the Kangal dog, to understand the effects of WBCs
transfused in two consecutive pRBCs on some immunological param-
eters and to detect the fundamental adverse reaction differences
between WBC reduced and nonreduced pRBCs on healthy recipients.
Kangal dogs (n = 100) have been tested for DEA 1.1 blood type fre-
quency, and 6 of them were chosen as donors. Healthy recipient mon-
grel dogs randomly assigned to either WBC reduced (n = 12) and non-
reduced (n = 12) groups, were compared on different blood withdraw-
al days (0, 10, 21, 31, and 42) for B lymphocyte, major histocompati-
bility complex (MHC) I and II, IgG, IgM expressions and neutrophile
function tests by using flow cytometry methods. Crossmatching tests
were performed prior both transfusions which were conducted accord-
ing to routine protocols, heart and respiration rate, body temperature
and capillary refill time were collected on 0th, 10th, 30th, 60th minutes
and 2nd, 3rd, 4th, 5th, 6th, 12th, 18th, 24th, 30th, 42nd, 54th hours post-transfu-
sion. The data sets were analysed statistically with Annova and repeat-
ed Annova. DEA 1.1 negative blood type was determined as 38.30%
in the Kangal dog, and 22.41% of the DEA 1.1 positive results were
minor agglutinations. No differences were noted for MHC I, II, IgG
and IgM expressions and for the phagocytosis, chemotaxy and oxida-
tive burst tests when comparing the effects between groups and days.
As expected we did not encounter transfusion reactions, during trans-
fusions that required its termination neither throughout the post-trans-
fusion observation period. The body temperature was statistically dif-
ferent beginning from the 3rd hour post-transfusion for both transfu-
sions (p < 0.001), the interaction between groups and posttransfusion
controls was significant only for the first pRBC transfusion (p < 0.05),
this was also significant in the evaluation of both transfusion data
analysed together (p < 0.05). In conclusion, DEA 1.1 negativity in
Kangal dogs has resulted in a low percentage, the minor agglutina-
tions observed for DEA 1.1 could be the result of a cross-reaction with
DEA 1.2 antigen and need to be further analysed. This study empha-
sizes importance on the monitorization. WBC reduction is not a com-
mon application in veterinary transfusion medicine; nevertheless pas-
senger WBCs might possess unreported detrimental properties.

A RESEARCH ON THE IMMUNOMODULATORY EFFECTS OF COMPATIBLE PACKED

RED BLOOD CELL TRANSFUSIONS IN DOGS - 601
G. Inal Gültekin, I. Akyazı, E. Aktas, Ü. Çötelioglu, M. Arslan, Y. Ziya OGRAK
E-mail: guldalinal@yahoo.com

The immunomodulation occurring in human transfusion medicine is
thought to be a result of the leukocytes (WBC) present in blood com-
ponents, and leukodepletion (LD) which is the reduction of WBCs
from blood components is suggested for several blood transfusion (BT)
situations. However, the probable effects of BT on the canine immune
system are insufficiently known. In this manner, the presented study
investigated the effects of WBC reduced compatible packed red blood
cell (pRBC) transfusion on the canine immune system of the recipients.
A complete blood count (CBC) and a differential count have also been
performed. The immune system of recipient dogs were investigated on
different blood withdrawal days (0, 10, 21, 31, and 42) for CD3+, CD4+,
CD8+, CD45RA+, CD4+CD45RA+ T lymphocytes and CD4/CD8 ratio
by using flow cytometry methods. The differences observed between
groups and days for the investigated immunological and haematologi-
cal parameters were not of statistical significance. With the exception
of CD3 + T lymphocytes, which decreased on day 42 compared to days
0, 10 and 31 (p ≤ 0.05). Moreover, the total WBC count in CBC and
the percentage of lymphocytes on differential WBC count, both
decreased in the WBC containing group compared to the WBC reduced
group (p ≤ 0.05). Mean corpuscular volume (MCV) and haemoglobin
concentration parameters decreased in the recipients receiving WBC
reduced pRBCs (p ≤ 0.05). MCV was also statistically higher on day
21 compared to the rest of the days (p ≤ 0.05). We found that the
immune system of healthy mongrel dogs did not react profoundly dif-
ferent to the pRBC transfusion whether it was WBC reduced or not.
Although, we have found that two consecutive compatible pRBC trans-
fusions led to a statistically significant decline in CD3+ T lymphocytes,
and a suppression of total WBC count and lymphocyte percentage in
recipients receiving pRBC with WBCs. In conclusion, the mild
changes observed in the immune system are yet far away of conclud-
ing an immunosuppressive effect of BT in healthy dogs. Nevertheless,
this study may provide the possibility to understand the immune
answer that takes place towards blood products containing WBCs. On
the other hand, longterm follow up and higher units of pRBC transfu-
sions are suggested for further understanding of the immunomodulato-
ry effects related to BT. Furthermore, WBC’s transfused in blood prod-
ucts might have different consequences in ill patients since they are
often immunocompromised.

120 Clínica Veterinária, Ano XIV, suplemento, 2009

34th World Small Animal Veterinary Congress 2009 - São Paulo, Brazil