N TAL &

IOWA’S ENVIRONMENTAL & PUBLIC HEALTH LABORATORY

ME PU
ON
R

BL
I V
The University of Iowa

CI
I OWA’S E N
HYGIENIC

H E A LT H
LABORATORY
The University

HYGIENIC LABORATORY
of Iowa

EST. 1904
LA
B O R AT O R Y

Mary J.R. Gilchrist, Ph.D., Director
George M. Breuer, Ph.D., Environmental Quality Division
Jane P. Getchell, Dr. P.H., Disease Control Division
George R. Hallberg, Ph.D., Information Services Division
Lee A. Friell, Des Moines Laboratory Manager
Stanton L. Berberich, Ph.D., Molecular Genetics
Beth M. Hochstedler, Training Coordinator
Stacia D. Freeburg, Environmental Laboratory Appraisal
Ted Heald,Laboratory Publications
M. Lynn Hudachek,Customer Services
Richard D. Kelley, External Program Consultant
Michael J. Loeffelholz, Ph.D., Molecular Biology
Nelson P. Moyer, Ph.D., Microbiology Consultant
Jane P. Getchell,Laboratory Certification (CLIA)
Norman S. Swack, Ph.D., Virology

Diagnostic and Reference Services

AFP SCREENING PROGRAM - Stanton Berberich
• Alphafetoprotein (AFP), human chorionic gonadotropin (HCG) and estriol tests
performed on maternal sera and AFP and acetyl cholinesterase tests performed on
amniotic fluids collected between 15-20 weeks gestation and submitted to UHL in kit
provided (319-335-4500)
AIR QUALITY - Marc Tucker
• Ambient air monitoring required by state and federal agencies
• Analyses for workplace and indoor exposures
ASBESTOS - Mary Richey
• Determination of asbestos in bulk materials, air and water by optical and electron
microscopy
BACTERIOLOGY - Karla Long
• Identification of referred cultures
• Clinical specimens cultured for gonorrhea, legionellosis, tularemia, brucellosis,
leptospirosis, Lyme disease, diphtheria, anaerobic infections, diarrheal disease,
streptococcal pharyngitis, pertussis, etc.
• Toxin assay forClostridium difficile, Clostridiumbotulinum
• Specimen kits available for fecal specimens and throat cultures. Pertussis and
diphtheria kits available by special request (see reverse)
• JEMBEC plates with Martin Lewis medium available to approved clinics for culture of
Neisseria gonorrhoeae
ENVIRONMENTAL MICROBIOLOGY - Nancy Hall
• Water testing for drinking safety (coliform bacteria and nitrate), conditioning problems
(iron, hardness and iron bacteria), recreational safety (coliform andPseudomonas
bacteria)
• Isolation and identification of agents of foodborne and waterborne illness
• Water testing forGiardia andCryptosporidium
• Water collection kits available
IMMUNOLOGY - Thomas Gahan
• Detection of antigens and/or antibodies for a variety of bacterial, fungal, parasitic,
rickettsial, viral, mycoplasmal and chlamydial agents
MOLECULAR BIOLOGY- Michael Loeffelholz
• Polymerase chain reaction (PCR) and DNA probes for detection of selected infectious
agents including HIV andB. pertussis (contact UHL)
• Plasmid analysis, ribotyping and other molecular epidemiological methods
MYCOBACTERIOLOGY - Dennis Gaunt or Karla Long
• Identification of referred cultures
• Isolation and identification of mycobacteria from sputum, bronchial washing, pleural
fluid, gastric washing, urine, tissue, CSF, wound aspirate
• Direct and indirect drug susceptibility testing
• Rapid identification and drug testing (Bactec, DNA probes, HPLC)
• Collection kits available for gastric, urine and sputum or other fluid specimens
MYCOLOGY - Dennis Gaunt or Karla Long
• Identification of referred cultures of molds, yeasts, actinomycetes,Nocardia
• Isolation and identification of fungi from sputum, bronchial washing, pleural fluid, CSF,
bone marrow, wound aspirate, tissue, skin, hair or nail scrapings
• Fungal serology (microimmunodiffusion test on single serum specimen) for histoplasmo-
sis, blastomycosis, coccidioidomycosis, aspergillosis, hypersensitivity pneumonitis
(farmer's lung)
• Specimen kit available
ORGANIC ANALYSIS - Pamela Kostle or Michael Wichman
• Analyses of solvents, formaldehyde, etc., in air, water, soil and other matrices
• Analyses for gasoline, diesel fuel in water and soil
• Pesticide residue determination and formulation verification
• Immunoassay screening tests (EIA) for private wells
• Determination of EPA Priority Pollutants, CLP Target Analytes, etc.
• Provides laboratory analyses to public water supply systems certified under the Safe
Drinking Water Act
OTHER SERVICES - DES MOINES LAB - Lee Friell or Richard Kelley
• Determines pollen counts for Des Moines and selected Iowa cities during ragweed
pollen season
• Provides laboratory support for statewide fluoridation program
• Bacterial analysis of surface water and wastewater (fecal coliforms)

INDUSTRIAL HYGIENE -Pamela Kostle or George Breuer
• Analyses for workplace chemicals, asbestos, indoor air pollution, etc.
• Collaborates with the Iowa Bureau of Labor for compliance monitoring and consultation
INORGANIC ANALYSIS - DES MOINES LAB - Vince Dwyer or Lee Friell
• Provides laboratory analyses to public water supply systems certified under the Safe
Drinking Water Act
• Determination of trace metals and minerals in water, soil, vegetation and selected
biological specimens
• Determination of lead and other metals in blood
• Analyses of solid wastes for flammability, corrosivity, reactivity, toxicity (TCLP) and
inorganic chemicals (RCRA program)
• Provides laboratory support for NPDES monitoring
IOWA NEONATAL METABOLIC SCREENING PROGRAM - Jan Susanin
• Screening for phenylketonuria (PKU), hemoglobinopathies (Hb), hypothyroidism,
congenital adrenal hyperplasia (CAH) and galactosemia performed on all newborns in
Iowa. All positive results are telephoned to attending physician. Specimen collection
forms and pamphlets available from the Des Moines Laboratory (515-281-7165)
LIMNOLOGY AND BIOASSAY - DES MOINES LAB - John Miller
• Conducts all phases of water, sediment and biological sampling and
• Bioassay for NPDES and toxicant monitoring
See Reverse Side for Specimen Kits &
Immunological Tests Available
PARASITOLOGY - Michael Last or Karla Long
• Identification of intestinal parasites (protozoa, helminths, etc.), blood parasites (malaria,
filaria, etc.), adult worms (ascaris, pinworm, etc.) and medically significant arthropods
(ticks, lice, etc.)
• Identification ofCryptosporidium andMicrosporidia
• Specimen kits available for fecal specimens (formalin/PVA) and pinworm examination
RABIES- Karla Long or Nelson Moyer
• Acceptable specimen is head or undamaged brain of suspect animal
• Delivery to Oakdale Hall M-F 8:00 a.m. to 5:00 p.m.; At other times call the UHL Duty
Officer at 319-335-4500 for instructions
• Results available within 24 hours after receipt of specimen and all positive results
telephoned immediately to physician or veterinarian upon completion of test
• Consultation available 24 hours by calling 319-335-4500
RADIOCHEMISTRY - Marinea Mehrhoff
• Determination of radium or other radionuclides in water, soil, vegetation, etc.
• Emergency field response teams for nuclear accidents
VIROLOGY - Sandra Jirsa
• Isolation and identification of viral, chlamydial, rickettsial and mycoplasmal agents
including human immunodeficiency virus (HIV)
• Serodiagnosis of HIV infection and selected chlamydial and mycoplasmal infections
• Antigen detection and identification of selected viral agents
• Specimen collection kits available
• Human lymphocyte immunophenotyping by flow cytometry for management
of HIV infection

FOR INFORMATION CALL (319) 335-4500 AIHA Accreditation: #31 NVLAP Accreditation: #1288
CLIA Certificate: #16D0648109 (Iowa City)
#16D0709301 (Des Moines) Blood Lead
Visit our site on the World Wide Web #16D0709302 (Des Moines) INMSP
www.uhl.uiowa.edu
 The University of Iowa • HYGIENIC LABORATORY
Designated by the Code of Iowa as the State Laboratory in Service to Any DES MOINES LABORATORY
State Agency, State Institution or Local Board of Health 900 E. Grand Ave. HA Wallace Bldg
IOWA CITY LABORATORY Clinical Laboratories Improvement Act Certificate Des Moines, IA 50319-0075
102 Oakdale Campus #H101 OH Accredited by the American Industrial Hygiene Association and the 515-281-5371
Iowa City, IA 52242-5002 National Voluntary Laboratory Accreditation Program Neonatal Screening
319-335-4500 Designated by the Environmental Protection Agency as the State P O Box 1803
Principal Laboratory for the Safe Drinking Water Act
Central Screening Laboratory for the Iowa Neonatal Metabolic Screening Program
Des Moines, IA 50306-1803
515-281-7165
Immunological Tests Recommendations for Shipment of Specimens
Test Specimen Transport Transport Collection
Disease/Infectious Agent Code* Code** Test/Specimen Medium* Temperature Kit No.

Adenovirus CF 3 b Alphafetoprotein Screening

9
Amebiasis IHA a Serum, Amniotic Fluid Ambient Inquire
3 4 12
Arbovirus (Encephalitis) CF ,EIA ,NT a,b Bacteriology
10
Aspergillosis MID a Feces (Salmonella, Shigella,Campylobacter) Cary Blair Ambient 8
3 11
Blastomycosis CF ,MID a Pharyngeal Swab (Group A Streptococci) Ambient 9
1
Brucellosis Agg a Nasopharyngeal Swab (Pertussis) Reagan Lowe Ambient 10
8
Cat Scratch Disease IFA a,d Wound Exudate UseCommerical Ambient
Chlamydia pneumoniae IFA 8 a Transport Medium
3 11
Coccidioidomycosis CF ,MID a Referred Culture Identification Slant Culture on Ambient
12
Coxsackievirus (Group B only) NT c Infusion Medium
10
Cryptococcal Antigen LA a Anaerobic Bacteria Chopped Meat Medium
9
Cysticercosis IHA a,d or Commercial Transport Medium
3, 4
Cytomegalovirus CF EIA a,b Special Culture Request and Toxin Assays Inquire
4
Dengue Fever EIA a,d Blood Lead
9
Echinococcosis IHA a,d Venipuncture/Capillary Blood 4 oC Inquire
12
Echovirus NT c Chlamydia Isolation & Identification
2
Epstein-Barr Virus ACIF a Aspirate (Epididymal, Lymph Sterile Container 4oC 1
4
Ehrlichia-HGE EIA a,d Node [specify], Tracheal) 2SP**
4
Hantavirus EIA a,d Swab (Conjunctival, Genital, Nasopharyn.) 2SP 4 oC 3
4
Hepatitis A Virus EIA a Tissue (Specify Site) 2SP 4oC 1,3
4
Hepatitis B Virus (surface antigen) EIA a Clostridium difficile Toxin Assay
4
Hepatitis C Virus EIA a Diarrheal Discharge (3-4 mL) Sterile Container -70oC (Dry Ice) 6
2
Herpes Simplex Virus CF b Environmental Chemistry & Hazardous Wastes
3 11
Histoplasmosis CF ,MID a Inquire About Tests Available and Kits Supplied
4
HTLV I/II EIA a Environmental Microbiology
4 15
Human Immunodeficiency Virus EIA , WB a Water (Drinking, Pool, Spa, etc.) Ambient 16,17***
11
Hypersensitivity Pneumonitis MID a Environ. Samples (Legionella, etc.) Contact UHL
3
Influenza Virus CF b Flow Cytometry
8
Legionellosis IFA b Whole Blood Contact UHL Contact UHL
1
Leptospirosis Agg a Molecular Biology
8
Leishmaniasis IFA a,d Whole Blood (EDTA) for HIV PCR
4 8 15
Lyme Disease EIA , IFA , WB b,d Nasopharyngeal swab for pertussis
3
Lymophocytic Choriomeningitis Virus CF b Mycobacteriology
3
Lymphogranuloma Venereum CF b Sputum, Aspirate, CSF Ambient 14
3 4
Measles Virus (Rubeola) CF ,EIA a,b Gastric, Urine Ambient 15
3
Mumps Virus CF b Referred Culture Identification Lowenstein-Jensen or
Mycoplasma pneumoniae CF 3 b Middlebrook 7H10
11
Nocardiosis MID a,d Mycology
3
Paragonimiasis CF a,d Sputum, Tissue, CSF, Aspirate Ambient 13
3
Parainfluenza Virus CF b Skin/Nail Scrapings, Hair Ambient 13
4
Parvovirus B19 EIA a Referred Culture Identification Sabouraud Dextrose Agar
3
Picornavirus (Echo & Coxsackie Virus) CF b Serum (See Immunological Tests)
12
Poliovirus NT b Mycoplasma Isolation & Identification
3
Psittacosis CF b Aspirate/Washing (Specify Site) Sterile Container 4 oC 1
8
Q Fever IFA b Swab (Genital, Nasopharyngeal, TPB** 4 oC 4
3
Respiratory Syncytial Virus CF b Placental, Throat)
8
Rocky Mountain Spotted Fever IFA a Tissue (Specify Site) TPB 4oC 1,4
4
Rubella Virus EIA b Urine (Urethritis) Sterile Container 4 oC 1
4
Schistosomiasis EIA a,d Neonatal Metabolic Screening
10
Sporotrichosis LA a,d Blood Specimen Collection Forms Contact UHL Ambient
9
Strongyloidiasis IHA a,d Parasitology
14 5
Syphilis VDRL FTA-Abs a Feces PVA/Formalin Ambient 11
4
Toxocariasis EIA a,d Blood Ambient
4 8
Toxoplasmosis EIA ,IFA a Rabies
4
Trichinellosis EIA a Head or brain of animal Ice Pack 4 oC
1
Tularemia Agg a Viral Immunology
8
Typhus IFA a Serum (acute and convalescent) Use table on left Ambient 5
Varicella Virus CF 3 b Virus Isolation & Identification
Blood, Stool, CSF Sterile Container 4 oC 1
*1. Agglutination; 2. Anti-complementary Immunofluorescence 3. Complement Swab† (genital, oral, nasal, rectal, etc.) TPB 4 oC 1,2
Fixation Test; 4. Enzyme Immunoassay; 5. Fluorescent Treponemal Antibody — Tissue (specify site) TPB 4oC 1,2
Absorbed; 6. Hemagglutination; 7. Hemagglutination Inhibition; 8. Indirect Washing†† (nasal, throat, etc.) TPB 4 oC 1
Fluorescence Antibody; 9. Indirect Hemagglutination; 10. Latex Agglutination; Urine Sterile Container 4 oC 1
11. Microimmuno-diffusion; 12. Neutralization Test; 13. Rapid Fluorescent Focus *Contact laboratory when submitting specimens for which no kit is provided.
Inhibition Test; 14. Venereal Disease Research Laboratory; 15. Western blot. **2SP = Sucrose phosphate solution; TPB = Tryptose phosphate broth
***UHL kitmust be used for water analyses. Specify whether water supply is chlorinated or nonchlorinated when
** (a) single serum; (b) acute and convalescent serum; (c) acute and convales-
ordering kits.
cent sera tested against patient isolate only; (d) specimen may also be referred † Swab specimen for Herpes and Influenza virus (Kit No. 2).
to CDC for testing. †† Washing may be obtained through the use of virus transport medium or sterile saline. TPB transport medium contains
antibiotics to retard bacterial contaminants.
7/96