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Onion Root Tip Meiosis Lab

Procedure

1. Place the slide onto the microscope and focus the lens to see the onion root tip
cells as clearly as possible
2. count cells in each phase
3. calculate to find percentage of the time each cell spends in each phase
4. record data in table

Analysis questions

1. If our observations had been of the entire root instead of just the tip, more cells
would have been in the interphase (about 90%) because cells would still be
maturing and elongating. There’d be less probability of other phases because the
apical meristem is the area specifically for cell division so it’s more likely to have
dividing cells.
2. A vast majority of time an onion root tip cell is spent in interphase because cells
aren’t constantly dividing- with the exception of anaphase (which is the shortest
stage), the time spent gets progressively smaller.
3. stages of mitosis in onion root tip cell

1 2 Total cells in Percentage of Time of cell in


each phase cells in each each phase
phase
White Fish Mitosis Lab

Stages of mitosis:

Interphase

Prophase

Prometaphase

Metaphase

Anaphase

Telophase and Cytokinesis


Analysis questions

1. To form two diploid daughter cells, a cell must go through mitosis. During
Interphase, the cell duplicates chromatin. Next, it goes through prophase, and the
chromatin become thick chromosomes and the nuclear envelope and the nucleoli
are no longer visible. The spindle first appears and attaches to the microtubules
and they start to move to opposite ends. In metaphase, the microtubules are at
polar ends and the chromosomes align at the center where the spindles attach to
each centromeres. In anaphase, the two sister chromatids of each pair part and
each chromatid becomes a chromosome and they start to move to opposite ends.
During telophase, two daughter nuclei begin to form and they are completely
identical. In the final phase called cytokinesis, the two daughter cells are
completely formed when the cytoplasm divides.
2. Animal cells separate during cytokinesis, and plant cells develop cell plates
between the two nuclei and create a new cell wall.
3. The centrosome organizes the cell’s microtubules, and they are necessary in cell
division because they form spindles that attach to the centromere which holds the
chromosomes together during mitosis or meiosis.