Khan Academy DNA Technology Questions 2.

In nature, restriction enzymes are meant

to protect
1. Plasmid G is cut with the restriction
enzymes EcoRi, BamHI, and HindIII in A. Humans from bacteria
various combinations. The sizes of the B. Bacteria from viruses
resulting fragments are seen in the table C. Bacteria from other bacteria
below. D. Viruses from bacteria

3. BamHI recognizes 6 base pairs of DNA

in a palindromic DNA sequence. If the
first part of the sequence is given below,
what is the rest of it?

A. 5’ CAA 3’
B. 5’ AAC 3’
C. 5’ TTG 3’
D. 5’ GTT 3’

4. A researcher has identified the location

A. of the gene that codes for protein X, and
has managed to excise it from the DNA.
She has also generated cDNA that
codes for the protein. If she runs these
two sequences through gel
electrophoresis, what should she expect
to see? (Note that the wells where the
DNA was loaded onto the gel are at the
top of the gels shown)

B.

A.
C.

B.
D.

C.
D.
5. A student was attempting to make cDNA
that would code for protein Y. They
began with an isolated and amplified
sample of the target post-transcriptional
mRNA, and all the tools required to
complete the task. However, at the end
of the experiment the student was left
with a molecule containing
ribonucleotides. What most likely went
wrong?
A. The student forgot to add DNA
polymerase
B. The student added RNA polymerase
instead of DNA polymerase
C. The cloning vector already
transcribed the DNA
D. The student forgot to add reverse
transcriptase
6. A student adds Taq Polymerase,
nucleotides, and primer to a test tube.
What procedure are they most likely
going to do next?
A. Southern Blot
B. Gel Electrophoresis
C. Microarray Hybridization Assay
D. Polymerase Chain Reaction
7. PCR (polymerase chain reaction) is an
excellent method of generating copies of
target DNA. If a single piece of double
stranded DNA (dsDNA) is put into a
PCR machine, how many dsDNA
segments will there be after 3 rounds?
A. 8 segments, with the 2 original
strands paired
B. 8 segments, with the 2 original
strands on different segments
C. 16 segments, with 2 original strands
on different segments
D. 16 segments, with the 2 original
strands paired
8. A scientist is trying to express gene X in
bacteria. After cloning the cDNA from
gene X into a plasmid with the
appropriate cDNA, and transfecting the
plasmid into bacteria the scientist
notices that he forgot to add antibiotics.
What will greet him the next day?
A. A mostly normal petri dish -
antibiotics are just used as a safe-
guard against other bacteria
B. A mostly normal petri dish -
antibiotics are just used as a safe-
guard against other bacteria
C. A petri dish with separate colonies
of transformed and untransformed
bacteria
D. A petri dish with only untransformed
bacteria, the antibiotics are required
for transfection
9. Which procedure is least effective for
detecting a specific DNA sequence in a
sample?
A. Microarray
B. Southern Blot
C. Polymerase Chain Reaction
D. Gel Electrophoresis
10. A DNA sample was sent off for Sanger
sequencing. The results are shown
below. What is the sequence of the
template strand?

A. 5’ ACTGACAGT 3’
B. 5’ ACTGACAGT 3’
C. 5’ TGACAGTCA 3’
D. 5’ ACTGTCAGT 3’
Khan Academy DNA Technology Answers

1. A
2. B
3. C
4. C
5. D
6. D
7. B
8. B
9. A
10. A