ZECEN Biotech Neo-1200

NEO-1200 User’s Manual

Taizhou ZECEN BIOTECH CO.,LTD

Version:1.1
ZECEN Biotech Neo-1200

Preface
Thank you very much for choosing the automatic biochemistry analyzer produced by Taizhou ZECEN
Biotech Co., Ltd. This manual provides the information about working principle, installation, operation,
maintenance and safety precautions for the instruments.

Important safety information

1. A complete understanding of how the instrument works is required before the user can safely operate
the automated biochemistry analyzer.
Before operating the system, please read this manual carefully and operate it only when you are
familiar with it.
2. The instrument is used for the serum, urine, cerebrospial fluid samples for clinical chemical analysis,
urinalysis, immunological examination. Other uses are prohibited.
3. For clinical examination, please accept the management of physicians or clinical laboratory
technicians, health inspection technicians who was trained by Taizhou ZECEN Biotech Co., Ltd.
4.In the process of using the instrument, it is necessary for a complete quality control management.
Monitor the operating status of the instrument periodically to determining the quality control, or it
may cause a false diagnosis.
5. Select the appropriate reagents, calibration solution and quality control solution, set the correct
project, calibration and quality control parameters, to preserve reagent, calibration solution and
quality control solution according to the provisions of methods.
6. If you change the reagent type or batch number, please correct the parameters and calibrate before
assay.
7. Mutual contamination between reagents will affect the determination results, please follow the
instructions required to give the appropriate settings.
8. Make sure that the sample contains no clots and no turbidity. Otherwise, the sample probe will be
clogged and will seriously affect the measurement results.
9. Some substances in the sample, such as drugs, anticoagulants, antiseptics, etc., can interfere
with determinations result.
10. Hemolysis, jaundice and chylomicrons will affect the determination of results, to do the blank
blank.
11. In accordance with the provisions of the preservation of the sample, inappropriate preservation will
change the structure of certain components.
12. Follow the instructions in this manual to operate and maintain the instrument regularly.

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13. Use the reagent as specified by the reagent supplier and set the correct measurement parameters.
14. The discharge of waste liquid must comply with the local environmental protection department.
Please consult the reagent supplier.

After-sales service and contact information

After-sales service:
Please contact the service department of Taizhou ZECEN Biotech Co.,Ltd

Contact information:
Company: Taizhou ZECEN Biotech Co.Ltd
Address: Fl. 1-4, No.62, Building G59, China Medical City, 225300, Taizhou
City, Jiangsu Province, P.R.China
Tel/Fax : 86-523-86201176

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Contents

NEO-1200 User’s Manual ............................................................................................................................. 1

Preface .......................................................................................................................................................... 2
Contents ....................................................................................................................................................... 4
Safety Precautions ........................................................................................................................................ 6
1 Brief introduction ................................................................................................................................... 8
1.1 About the manual ......................................................................................................................... 8
1.2 Introduction .................................................................................................................................. 8
1.3 Users............................................................................................................................................. 8
1.4 Security ........................................................................................................................................ 8
1.5 General precautions ...................................................................................................................... 8
1.6 Electricity safety precautions ....................................................................................................... 9
1.7 PC Precautions ............................................................................................................................. 9
2 Working principle ................................................................................................................................. 10
2.1 Fundamentals ............................................................................................................................. 10
2.2 Hardware .................................................................................................................................... 10
2.2.1 Optical path system ....................................................................................................... 10
2.2.2 Circuitry ........................................................................................................................ 11
2.3 Software ..................................................................................................................................... 12
2.4 Structure of the composition ...................................................................................................... 13
2.5 Product Scope............................................................................................................................. 13
2.6 Collection of samples ................................................................................................................. 13
2.7 Technical parameters table ......................................................................................................... 13
2.8 Installation requirements ............................................................................................................ 14
2.8.1 Installation Environment ............................................................................................... 14
2.8.2 Temperature and humidity ............................................................................................ 14
2.8.3 Power Supply and Grounding ....................................................................................... 14
2.8.4 Deionized water ............................................................................................................ 14
2.9 Standard configuration ............................................................................................................... 14
3. Overview of Instrument Installation....................................................................................................... 16

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3.1 Instrument description ................................................................................................................ 16

3.1.1 Hardware ....................................................................................................................... 16
3.1.2 Software ........................................................................................................................ 16
3.2 Instrument installation ................................................................................................................ 16
3.2.1 Unpacking the instrument ............................................................................................. 16
3.2.2 Instrument installation................................................................................................... 16
3.3 Instrument installation flowchart ............................................................................................... 17
4 Daily operation ..................................................................................................................................... 18
4.1 Flowchart.................................................................................................................................... 18
4.2 Sample assay .............................................................................................................................. 19
4.2.1 Preparation before assay ............................................................................................... 19
4.2.2 Power on ....................................................................................................................... 19
4.2.3 Test samples .................................................................................................................. 19
4.2.4 Sample information registration.................................................................................... 21
5 Items setting ......................................................................................................................................... 22
5.1 Reagent location settings ............................................................................................................ 24
5.2 Calculate item settings ............................................................................................................... 25
5.3 Item group management settings................................................................................................ 26
6 Standard quality control management..................................................................................................27
6.1 Calibration management .............................................................................................................27
6.2 Quality control management ...................................................................................................... 28
7 Results query ........................................................................................................................................ 32
7.1 Result Query............................................................................................................................... 32
8 Maintenance ........................................................................................................................................ 33
8.1 Instrument Initialization ............................................................................................................. 33
8.2 Instrument maintenance ............................................................................................................. 33
8.2.1 Daily maintenance......................................................................................................... 33
8.2.2 Weekly maintenance ..................................................................................................... 33
8.2.3 Maintenance every six months ...................................................................................... 33
8.2.4 Annual Maintenance ..................................................................................................... 33
8.2.5 Reagent, probe, and probe Exchange ............................................................................ 33
8.3 Instrument assay ......................................................................................................................... 34
9. Troubleshooting...................................................................................................................................... 35
Annex 1 packaging and storage logo .......................................................................................................... 36
Attachment 2:Meaning of signs ..................................................................................................................37

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Safety Precautions

Automatic biochemistry analyzer is a precision instrument, please pay attention to the following points
before use:

The instrument must be transported in the following environmental conditions:

Environment temperature: 4℃~ 40℃;
Relative humidity: 30% ~ 80%;
Atmospheric pressure: 860hPa ~1060hPa;

The following operating conditions must be observed when installing the instrument:
Power supply: 100-240VAC, 50/60Hz, 102W
Temperature: 15℃~ 30℃;
Relative humidity: 40% ~ 85%;
Away from strong electromagnetic interference, strong grounding is suggested;
UPS power supply can not be less than 1KW;
Installation must be carried out by the manufacturer of professional engineers;
NE0-1200 automatic biochemistry analyzer is a highly specialized precision instruments, please prohibit
non-professionals engaged in maintenance and maintenance work, otherwise the company shall not be
responsible for the arising consequences.

When the instrument has high temperature and high voltage, please pay attention to avoid the

personal safety hazards.

The reagents and samples may be corrosive or contagious. Please pay attention to the protection

and concentration of the waste liquid when operating the instrument.

When the instrument has many moving parts in the work, pay attention to the hands and do not

touch the moving parts in the boot to avoid personal injury.

Notifications:
ZECEN Biotech does not make any form of guarantee. ZECEN Biotech does not assume responsibility, for
errors contained herein, or consequential damages resulting from the furnishing, performance and use of this
manual.
Manufacturer's responsibility:
The safety and reliability of the instrument is guaranteed by Taizhou ZECEN Biotech Co.Ltd under the
following conditions: The operation, expansion, re-adjustment, modification and repair are carried out by the
authorized personnel of the company. And the instrument is used in accordance with operating instructions.

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ZECEN Biotech Neo-1200

ZECEN Biotech provides the circuit diagram to be charged, and a list of calibration methods and other
information to help users to repair the related part.
Process assurance:
ZECEN Biotech ensured that the instrument in normal use and maintenance state, from the date of the
installation within one year, ensure there is no failure from the production process and raw material .

Safety, reliability and operating condition:

The company is not responsible for the safety, reliability and operation of the instrument if:
 The components are disassembled and re-commissioned;
 Non-authorized personnel to repair or modify the instrument.

Please read the instruction manual carefully and keep it in good condition.

Before proceeding, please read the safety instructions carefully and fully understand its meaning.
For ease of access, please keep this manual at user’s side.

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ZECEN Biotech Neo-1200

1 Brief introduction

1.1 About the manual

Before using the instrument, please read this section carefully, which includes important information on
using the instrument .
This manual can help you operate the instrument safely and correctly. To achieve this, the manual
describes the components of the instrument and how it works. The manual introduces software and hardware
information in details.
After understanding the components of the biochemistry analyzer, how to use these modules to complete
the routine work step by step is described.
Some warnings, hints, and useful notes are highlighted by key chapters that have eye-catching "attention"
tags.
This manual applies to NE0-1200 automatic biochemistry analyzer.

1.2 Introduction

NE0-1200 automatic biochemistry analyzer is produced by Taizhou ZECEN Biotech Co,.Ltd., carrying
40 cuvettes, characterized by the moderate analysis speed, no cross-contamination, less consumables, open
system and etc.
Neo-1200 is a routine instrument of the hospital, and it is used to test the patient's body fluid in a variety of
biochemistry indicators. At present, our analyzer, whether from the instrument's accuracy, manufactucircle
accuracy and its functions have reached the same level of foreign countries. NE0-1200 automatic biochemistry
analyzer uses a discrete cuvette, the cuvettes are separated from each other without cross contamination, the
accuracy and speed of the instrument has been greatly improved, to overcome the flow of the cuvette, serious
cross-contamination, which is welcomed by the majority of hospitals.

1.3 Users

NE0-1200 fully automatic biochemistry analyzer must be operated by trained personnel according to the
operating instructions.

1.4 Security

The following sections describe safety, electronic precautions, and other related potential hazards.

1.5 General precautions

When using the automatic biochemistry analyzer, user should strictly abide by good laboratory standards,
wear appropriate protective clothing and rubber gloves.
During the operation of the NE0-1200, the moving parts move very quickly, so do not put your hand in the

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working area to avoid accidental injury. Do not lean or tilt the instrument.

1.6 Electricity safety precautions

When removing an instrument part or circuit board, first turn off the power and disconnect the power
supply.

1.7 PC Precautions

To prevent PC viruses, the PC can not be used for other purposes.

Please use the running software of NE0-1200 automatic biochemistry analyzer written by Taizhou ZECEN
Biotech Co.,Ltd.

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2 Working principle

2.1 Fundamentals

According to Lambert-Beer law, a beam of incident light go through the colorimetric solution; the incident
light, transmitted light and the solution of light absorption has the following law.

A = -Log10T = KCL
T = I / Io
Io ---- incident light intensity
I ----- transmitted light intensity
A ---- Absorbance
C ----- solution concentration
L ----- optical path length
K - ----coefficient
The principle is light absorption: Lambert - Beer's law; monochromatic light go through the reactant
solution, and through the absorbance to determine whether physiological indicators of the human body is
normal.

2.2 Hardware

In order to complete the assay automatically, the instrument must have a certain amount of hardware
support, including the following aspects.

2.2.1 Optical path system

1. Light source 2. Cuvette 3. Photoelectric receiver 4. Photovoltaic cell 5. Photoreceptor

Instrument optical system is shown as above.
The light from the light source enters the cuvette and enters the optoelectronic receiver through the solution.
Light source is cold light source, use rectangular structure, through the UV plexiglass cuvette, (please

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ZECEN Biotech Neo-1200

ensure cuvette uniform, translucent hole can not have any scratches,) accurate 40 aliquot light transmission
groove aligned cuvette Of the middle position, to ensure that the sampling points in the middle of the cuvette;
reaction tray circle placed a total of 40 cuvettes, the minimum amount of 180ul.
The advantage is that there is no cross-contamination, the rotation of the colorimetric disc when the
colorimetric speed, we use a static spectroscopy system, the application of imported LED and photoelectric
receiver components, the test wavelengths were 340, 405, 450, 492(optional), 510, 546, 578(optional), 630,
the spectral half-width of less than 10nm ± 2nm, this can be a collection of eight wavelengths of data without
any mechanical movement, to overcome some defects of some foreign products.To ensure the measurement
accuracy.

2.2.2 Circuitry

Sample arms control Reagent panel

PC connection interface circuit

dircui control circuit

Injector control

PC system
circuit
Data acquisition
circuit

Color dial control Sample tray

circuit control circuit

Block diagram of the whole machine as shown above.

We use ARM main control chip to control a stepper motor, control the stepper motor direction, speed,
rotation steps, through the serial bus connection, and then through the USB interface with the PC. The
sampling arm has two stepping motors to control the rotation and the lift. The two position sensors detect the
position of the lifting arm and the rotation of the sampling arm respectively. The liquid level sensor detects the
sample and the reagent liquid level. The data acquisition circuit uses independent pre- 16-bit A / D converter
converts analog signals into digital signals, and the cuvette dial control circuit has a stepping motor drive
control, a cuvette position sensor, a heating power source and a temperature control circuit , The light
source of the power supply. The reagent tray control circuit has a stepper motor control to drive, the
syringecontrol circuit is a stepper motor driven sycirclee, the sensor senses the original position of the

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sycirclee, a solenoid valve controls the washing and addition of the sample, and the peristaltic pump
provides a purge .The sample tray control circuit is composed of a stepper motor plus a position sensor. The
position sensor is a light sensor.
The interface circuit is composed of a single chip microPC and some peripheral circuits, which can
convert the sent data from the PC into all the moving parts. The digital signal and the instrument status
information of the A / D converter are sent to the PC processing at the same time.

2.3 Software

Software consists of two parts, one is to calculate the operating software, and the other is the
microcontroller control software.
The user interface is completed in the Windows7 platform and above, while the database using access form.
In a unified user interface, the sub-module is to complete a specific function.
Routine test menu for daily work should be completed every day, which has several sub-menu modules:
patient information entry, test item selection, and sample testing, which is the most commonly software
modules; The software supports STAT, which prioritizes some of the programmed samples; show the results of
each sample in real-time; the reaction curve can display the curve of any test items; The reaction process curve
is easy to understand the test details; the results treatment is to print the results of the sample.
In addition to daily special test, user can also do the standard sample test: drawing standard curve; test QC,
QC diagram description; which also can test the performance of the instrument: linear measurement,
absorbance measurement, repeatability measurement; Reagent position arrangement is for the location of each
test item, so that the instrument can accurately find the required reagents. Maintenance and repair submenu is
to facilitate the maintenance engineers of the various components of the instrument, in order to identify the
fault.
Parameter setting menu is to complete the parameters of the preparation of the module; test item sub-menu is
mainly for sample size, reagent volume, wavelength, test methods and etc.; Special item parameter settings is
to set a calculation formula , according to the results of the test, it has been calculated to obtain a new item
results; through the combination of item settings, it is to facilitate the items setting.
The data processing module can inquire the test results and QC results of the patient at will. The
mathematical statistics, daily mean value calculation and trend graph of these results can provide valuable
statistical data to the lab. The instrument fault record can record the various fault information.
Reagent location module, is mainly on the reagent location settings, with different reagent items for fixed
location settings, to facilitate the daily operation of hospital staff.
Instrument calibration module is mainly for the staff to provide a means of testing instrument, which
includes the calibration of the moving parts and the optical signal test.

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2.4 Structure of the composition

Biochemistry analyzer consists of host, PC (including the PC host and monitor, Windows7 or above
operating system and special software), printers and other accessories.
PC and printer are purchased by users, we only provide recommendation.
Minimum configuration: the PC clocked not less than 500MHz;
RAM is greater than 128M;
Hard tray capacity is greater than 40G;
Monitor is 15 inches or more, the resolution is not less than 1024 × 768;
the main accessory of biochemistry analyzer host:
Sampling part: reagent tray, sample tray, sample arm.
assay: optical systems, signal acquisition.
Reaction: reaction tray, constant temperature system, washing system.

2.5 Product Scope

This product is suitable for medical biochemistry analysis and testing.

2.6 Collection of samples

Samples must be fresh samples collected the same day, and the samples were centrifuged to separate the
serum , which can not be hemolytic and solidification.

Note: samples may be contaminated with infectious bacteria, the treatment must be well protected samples,
and waste must be with centralized treatment.

2.7 Technical parameters table

Instrument Type: Discrete

Test speed: 120T / H
Test method: end point method, fixed time method, dynamic method
Measurement wavelength: 340 ~ 630, 8 wavelengths
Light source: monochromatic light, cold light source
Reaction: 48 special materials produced by the cuvette
Temperature of cuvette: 37 ± 0.1 ℃
Sample positions: 31
Sample volume: 5 ~ 50μl
Reagent Positions: 32(double reagent bottle)
Reagent volume: 150 ~ 400μl
Reagent probe with liquid level assay, crash safety protection
STAT: can be inserted STAT at any time and add assay

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Calibration: linear / non-linear multi-point calibration

Quality control: free to insert quality control, can store, display, statistics and print quality control chart
Software: Windows environment to run, the system easy to operate
Print: a variety of print formats are free to choose
Power supply: 100-240VAC, 50/60Hz，102W
Weight: about 13KG

2.8 Installation requirements

2.8.1 Installation Environment

1. Avoid direct sunlight

2. Less dust, small vibration , low noise
3. Far away from a strong source of interference
4. Good grounding, and grounding resistance cannot be less than 4Ω;

2.8.2 Temperature and humidity

1. Room temperature should be between 15-30 ℃

2. Relative humidity: not more than 80%
3. Well ventilation

2.8.3 Power Supply and Grounding

1. Power supply: 100-240VAC, 50/60Hz

(Recommended power supply or UPS power supply)
2. Input power: 102W
3. Grounding resistance: <4Ω

2.8.4 Deionized water

3 liters per hour, the conductivity is less than 2.0us / cm.

2.9 Standard configuration

No. Name Unit Quantity remarks

Automatic biochemistry
1 PCS 1
Analyzer

2 Manual PCS 1

3 Acceptance report PSC 1

4 Certification PCS 1

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ZECEN Biotech Neo-1200

5 Warranty card PCS 1

6 Packing list PCS 1

7 Operation software(CD) PCS 1

8 Reagent tray PCS 2

9 Sample cuvettes PCS 300

10 Cuvettes PCS 80

11 Reagent bottoles Set 32

12 USB communication cable PCS 1

13 Power Adapter SET 1 With power cord

14 Outlet pipes PCS 2 1.5m

15 Import pipes PCS 2 1.5m

16 Ground wire PCS 1 2m

17 Clump weight PCS 2

18 Allen wrench SET 1 1.5-6

19 screwdriver PCS 1 Short doublehead

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ZECEN Biotech Neo-1200

3. Overview of Instrument Installation

3.1 Instrument description

3.1.1 Hardware

(1) Host
NE0-1200 automatic biochemistry analyzer does not need any manual work during the whole process.
(2) PC
All the operation and control of this instrument are carried out under the control of the PC, which is
connected to the host through the USB cable line, the PC directly affects the system quality, so the PC must
be a good brand machine, which must meet the system requirements; In order to install the system software,
the PC must also be equipped with CD-ROM. The PC should be a dedicated instrument, not allow to do other
work.

Note: Please do not use the PC for other purposes!

3.1.2 Software

The instrument is equipped with a set of system software CD, the system software is based on the Windows
7 platform, so the PC must be installed Windows 7 or above operating system.

3.2 Instrument installation

3.2.1 Unpacking the instrument

The instrument is a valuable precision instruments, in the transport and handling, it should be careful to
handle and can not be inverted; Check the box is complete or not before installation, whether damp or not, if
the above circumstances happened , need to notify the relevant personnel to deal with.. Unpacking steps are as
follows:

(1) Remove the carton for packaging;

(2) Remove the moisture-proof plastic sheet;
(3) Check whether the surface of the instrument is abnormal;

3.2.2 Instrument installation

(1) Installation of water tank;

(2) Installation of waste water tank;
(3) Connect the inlet pipe and the waste pipe;

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ZECEN Biotech Neo-1200

(4) Install the PC;

(5) Connect the power cable and serial cable;
(6) Add deionized water into the bucket

Note: Outer pipe into the waste tank is not too long, it is advisable to 10 cm or so. Avoid excessive waste
water to cause poor drainage!!

3.3 Instrument installation flowchart

Item parameters
Setting
Reagent position setting

Calibrator setting/Run
Calibration QC fail
Select calibration in correct

Test QC as sample
QC
QC setting/Run

Ready

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ZECEN Biotech Neo-1200

4 Daily operation

4.1 Flowchart

Starting up

Preheat 20 minutes
Clean all cuvette
Y

Calibration
N

Run calibration

Y
N
QC

Run quality control

Clean all cuvette

Input sample information

Sample assay

Test completed

Report result

Shutdown

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ZECEN Biotech Neo-1200

4.2 Sample assay

4.2.1 Preparation before assay

(1) Sample preparation

Blood samples should be centrifuged, and the serum can not have clots, hemolysis and other phenomena,
and add the serum into sample cuvette, make sure the sample can not have bubbles in the sample cuvette.
(2) Reagent preparation
To add a sufficient amount of reagents into reagent cuvettes, and reagent should be within the validity
period and reliable.
(3) Preparation of purified water
Requires deionized water , conductivity of less than 2.0us / cm of.
(4) Check before the boot
Check the main voltage is normal.

4.2.2 Power on

(1) Open the instrument cover, and the probe is adjusted to the position of the washing pool, and the
washing probe is mentioned above the colorimetric cuvette.
(2) Turn on the main power switch (located on the left side of the rear panel of the main unit).
(3) Double-click the shortcut icon to enter the user name "admin", password "admin", and the
instrument will boot automatically, please check the probe, reagent probe, mixing probe be in the center , and
4 washing probes should be directly above the corresponding cuvettes.
(4) The host machine is ready for testing after 20 minutes of warm-up.

4.2.3 Test samples

(1) Double-click the shortcut icon to enter the software interface as follows:
(2) Click "Sample Information" to enter the following interface:

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A. Click the "Add" button, and the sample number is automatically generated, users can also enter the
sample number by themselves. (Sample number can only have a unique number, which can not be repeated).
B. Select the sample type and cuvette type.
C. Select the corresponding department in the drop-down menu of "Inspection Departments".
D. From the drop-down list, select the position of the sample (this position is where the sample is placed in
the sample tray and can be repeated).
E. Select the doctor;
F. Select whether STAT;
G. Select the test items;
Single Sample Select the item to be tested in the optional item or click "item Portfolio". The item will
appear in the selected item field and click Save.
Click "Add batch", double-click to select the items to be tested, set the starting position and the number of
samples, click the batch to add, and then exit the batch add window.
（3）click the "test" to enter the following test interface:

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Click "Start" to start the test.

4.2.4 Sample information registration

After the test, click "OK" button to exit the sample assay window, click on the results of the query
displays the following interface:

Select the sample number in the result box, and the result will be displayed in the result column. Click
"Edit" to enter the sample patient information according to the sample number. Click "OK" when finished.
If you need to print, select the sample number you want to print, enter the patient information and click
Print.

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5 Items setting

When you need to carry out new projects or adjust the introduction of new reagents for item testing, you
must first set the item parameters, enter the "Items Setting" interface:

The item number, item name, item code, sample size, reagent amount, wavelength and other parameters
of the test item are displayed.
Sample size, the amount of reagents, the amount of two reagents, these data will set the item to absorb the
sample size and reagent volume; which the input range of sample is 3-50ul, the best amount of first reagent
range is 180-300ul, second reagent amount is 10-150ul. If a second reagent is not available, enter "0" in the
second reagent field or do not enter data.
The test parameters have the following parameters:
Test wavelength: 340, 405, 450, 492(optional), 510, 546, 578(optional), 630; Measurement method: end
point method, fixed time method, dynamic method.
According to the test reagent and method selection;
Typical reaction curves are as follows:
OD OD
2.0 2.0

1.0 1.0

Figure 10. The finish method reaction curve diagram Figure 11. Schematic of the kinetic reaction curve

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ZECEN Biotech Neo-1200

Slope: Absorbance is increasing when reading, that is positive slope

Absorbance is decreasing, that is negative slope
Reading point: The main reading segment refers to the time during which the reading begins and the time
period of the reading during the test;
Note:
1. 31 points will be read for one sample test, 32 seconds apart between each point, so for one sample
real test time is 31×32=992 seconds.
2. 1th period add R1, 4th period add S, 16th period add R2.
3. If mono reagent the startpoint=incubation time/32+4; endpoint= read time/32 +startpoint
4. If double reagent the startpoint= incubation time/32+16; endpoint= read time/32 +startpoint
5. Kinetic reading point has to more then 6, so of kinetic endpoint less than 6, please add it at less 6
points.
If the sample blank method is to be used during the test, you need to read the sample blank. We use the two
point reading, the auxiliary reading to read the sample blank data, the main reading section to read the reaction
data, and to process the data according to the need.
Such as: auxiliary reading for the beginning of the paragraph 13, the end of 14, the main reading section of
the starting point of 28, the end of 29 schematic diagram is as follows:

Figure 10. The finish method reaction curve diagram

Male / Female Reference Range: refers to the normal range of test results, beyond the scope of the instrument
can be given at the test results;
Unit: Unit of concentration value, which can be selected in the drop-down list;
Decimal places: the decimal point of the test results, according to the need for input, commonly used 2 decimal
places;
If you want to modify the parameters of the project, select the item in the item list, select the content you want
to modify, and click Save to store the modified parameters.
If you want to delete a test item parameter, select the item in the item list and click the "Delete" button to delete

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the item parameter.

To add the test items, click the "Add" button, you can set the test parameters and save.

5.1 Reagent location settings

In the process of entering a new test item or changing a new one, you will need to change the test
parameters and proceed to the reagent position setup as follows:
Click the main interface of the "reagent position" menu, enter the following interface:

Click the "Add" button to add the reagent location for the item.
In the "Item Code" drop-down box to select the needed item to add the reagent location of the project. If you
select "ALT", select "1" or "2" in the "Reagent Type" drop-down box,
In the Location Number field, enter the location for the reagent bottle "1" or "2" and click "Save.
You need to delete the item, click the corresponding item on the right, click "Delete".
Instrument Reagent trays can hold up to 16 reagent vials. The reagent discharge order must be set in the
Reagent Position interface.

Note: the reagent position of the instrument can not be misplaced, the reagent test to be done before testing

to confirm that there are sufficient reagents and then to start the item testing.

Reagent set inner circle for reagent R1, outer circle for reagent R2. When setting double reagent,

we should send the same position but different type in R1 and R2.

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5.2 Calculate item settings

Click the main interface in the "Item Parameters" in the "Item Information" menu, enter the following
interface:

In the Calculation Item Information setting;

Click "Add", the item number will be generated automatically. Input the item code, item name, select the unit
in the Unit drop-down menu, enter the reference range, enter the formula, and click Save.
Delete: Delete the corresponding calculation item.
Close: Exit to return to the main interface.
Add: Add item.
Save: Saves the set items.

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5.3 Item group management settings

In the item information, select the item whose combination you want to set.
Click "→" to select the desired item in the "To be added" box.
Enter the item Group Name.
Click "Save" to save the item group.
Click "Delete" to delete the unneeded items.

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ZECEN Biotech Neo-1200

6 Standard quality control management

6.1 Calibration management

Some test items need to be calibrated before testing. Click the "Calibration Management" menu in the
main menu to enter the following interface:

（1）Before calibration, you must first set the item calibration parameters, select the name of the item
to be tested, then the item code and units will be displayed, select the calibration method in the calibration
method, enter the standard lot number, click “ add”, in the parameter information it will display the items
which needs to be calibrated, enter the calibration solution concentration and calibration fluid placed cuvette
number, click "Save", the item is displayed on the target to be measured interface, click the "start ", then to
start the calibration test.
（2）After the calibration test is completed, click the "calibration management" in the main interface of
the "calibration history", enter the following interface:

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ZECEN Biotech Neo-1200

Select the item name in the calibration history table, and click "set to current";
Double click the item name in the calibration history table, pop up the edit calibration factor window, you can
view the calibration curve and modify the factor.

6.2 Quality control management

In order to monitor the accuracy of test results, and sometimes user must test some sample quality control,
there are a few methods for quality control, as a common sample test to see whether the test results are in the
control range, the second is to enter the quality control application interface to go on quality control test.
（1）click on "quality control", enter the quality control management interface:

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ZECEN Biotech Neo-1200

Before doing the quality control, you must first set the basic information:
Click “Add”.
Enter the name of the quality control solution.
Enter the lot number of quality control solution.
Select the location of quality control solution.
Set the validity period.
Click "Save" to add the batch control solution.
（2）In the "Item list information" column, select the test items to be added to the "control items" column;
In the "Control Items" column, click an item and enter the average concentration and standard deviation of the
item;
Click "Save" to save the settings of the item;
Click "Close" button to return to the main interface;
Click the main interface in the "Quality Control" in the "quality control application" menu, enter the
following interface:

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ZECEN Biotech Neo-1200

Select the control solution to be tested in the quality control solution. The name of the item set in the control
solution will be displayed in the list of detectable items. Select the item to be tested and click "Add". The item
will be added to the " Test item "list, click" start assay ", the following quality control testing window will
pop up.

Click "Start " in the window, the instrument starts QC test.

（3）Click the main interface in the "Quality Control" in the "Quality Control Management" menu, enter the

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ZECEN Biotech Neo-1200

following interface:

Select the batch in the QC list, select the time period, and click Query to query the corresponding batch QC test
chart.

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ZECEN Biotech Neo-1200

7 Results query

7.1 Result Query

Click "Result Query" to enter the interface:

Select the start and end dates and click Query to query the sample information.

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ZECEN Biotech Neo-1200

8 Maintenance

8.1 Instrument Initialization

(1) Turn on the automatic biochemistry analyzer switching power supply, enter into automatic
biochemistry analyzer software, the instrument automatically initialized.
(2) during the test, the moving parts of the instrument will be shifted. You can click "Calibration", input
the password "123456" and click the "Initialize" button to reset the instrument.

8.2 Instrument maintenance

8.2.1 Daily maintenance

(1) Check the cuvette assay surface, timing replacement of scratches and dirty cuvette.
(2) Check the washing bucket, emptying the waste tank.
(3) Check the probe position and probe injection probe for clogging.
(4) Check the washing port for foreign matter blockage.

8.2.2 Weekly maintenance

(1) Scrub sample probe / reagent probe with alcohol .

(2) Wash the instrument surface.
(3) Wash the reagent carbon.

8.2.3 Maintenance every six months

(1) Check the light source is normal.

(2) Check the injection pump is working properly.
(3) Check if the syringe is leaking and replace the syringe if necessary.

8.2.4 Annual Maintenance

(1)Check the power supply voltage and grounding line is in good contact.
(2)Mechanical arm maintenance.
(3)Waterway pipe replacement.
(4) Mechanical position adjustment.

8.2.5 Reagent, probe, and probe Exchange

If you accidentally damage the reagent probe (syringe probe or mixing probe), it needs the manufacturer and
professional engineers to replace and adjust.
(1) Carefully remove the reagent probe (syringe probe or mixing probe) top cover.

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ZECEN Biotech Neo-1200

(2) Replace a new reagent probe (syringe or probe) .

(3) Fix the reagent probe (sample probe or mixing probe) .
(4) Use automatic biochemistry analyzer software calibration procedures, to adjust the reagent probe
(plus probe or probe) position.
(5) Fix the top cover.

NOTE: The position of the reagent probe (syringe or probe) must be adjusted. Otherwise, the new reagent

probe (syringe or probe) may be damaged.

8.3 Instrument assay

The instrument fails, reboot automatically initialized, if problems can not be solved, please record the
symptoms and causes of failure in detail , contact with the company maintenance engineers.

NOTE: The instrument calibration procedure must be performed by a person, who knows how to operate

the instrument .

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ZECEN Biotech Neo-1200

9. Troubleshooting

(1) No reaction after power on;

Check the power plug.
(2) The lamp is not on;
Check the light source and make sure whether the lamp is burned out, such as the light source is bad to
replace the light source.
3) Sample probe was not washed;
Check whether the flushing pump is working. If normal, check the water connection pipe, such as fall
off, then connect the pipe; if the pipeline is blocked replace the pipe.
If the flushing pump is working abnormally, replace the water pump.
(4) Sample volume is not accurate;
Check whether the syringeleakage, or connecting the pipeline is leak, or probe is blocked.
Then replace the tubing or sycirclee.
(5) Probe position deviation
The position of the syringeprobe is adjusted by the instrument software.
(6) Item test results are not accurate
Check the reagents and sample to observe with the same wavelength of other items are normal, if are not
normal, it is possible to replace the LED lights.
(7) If the serial port is wrong or the data is not uploaded during the sample test, immediately exit the test
interface, re-insert the PC-side serial port once again, and then re-test the sample.

Note: If the fault can not be excluded, please contact our service engineer.

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ZECEN Biotech Neo-1200

Annex 1 packaging and storage logo

1. Up: indicates that the correct position of the transport package is upright

2. Moisture: aviod rain

3. Be cautious: transport package handling should be handled with care

4. In-vitro Diagnostic

5.Weight: 13kg

6.Dimensions: 540mmX445mmX440mm

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ZECEN Biotech Neo-1200

Attachment 2:Meaning of signs

If user ignored this sign or made mistakes, they may be injured, or it cause damage to the
occurrence of the object.

Note Indicates the content that user should pay attention to in order to maintain the
NOTE
instrument's performance and prevent damage.

Protective conductor terminals

Biological risk

In-vitro Diagnostic

High-voltage danger

Handle carefully

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