the kinetics of enzyme ribonuclease is measured.

Calculate km and vmax value from the following datA

[M]S V [{MICROMOL] 1/S 1/V
2.5E-06 28 400000 0.035714
4E-06 40 250000 0.025
1E-05 70 100000 0.014286
2E-05 95 50000 0.010526
4E-05 112 25000 0.008929 0.04
0.0001 128 10000 0.007813 0.035
f(x) = 7.1519968
0.002 139 500 0.007194 0.03 R² = 0.99996035
0.01 140 100 0.007143 0.025

Axis Title
0.02
0.015
0.01
Vmax 0.0071 140.8451
0.005
9.86E-06
km 700E-08x 1.0*10-5M 0
0 100000200000
Axis T
 Chart Title
0.04
0.035
f(x) = 7.15199685292932E-08 x + 0.007105300407768
0.03 R² = 0.999960353419263

0.025
Axis Title

0.02 Column E
Linear (Column E)
0.015
0.01
0.005
0
0 100000200000300000400000500000
Axis Title