Science Investigatory Project  Describe the research design (or defend)

Parts  Provide enough details so that a competent

worker can repeat the experiments

 Title
Materials and Equipment
 Table of Contents
 Overview of the Experiment
 List of Figures and Tables
o Population/Sample location
 Abstract
o Restrictions/Limiting conditions
 Research Plan
o Sampling technique
 Materials and Methods
o Materials
 Treatments / General procedures
 Introduction  Treatment / General Procedures

 Background of the Study o Procedures

 Statement of the Problem o Variables / Groups
 Significance o Statistical Treatment
 Scope and Limitations
 Review of Related Literature
 Related Readings and Literature (Conceptual)
 Related Studies (Research)
 Results and Discussion
 Conclusions
 Recommendations
 Bibliography
 Acknowledgments

General Guidelines in Writing Research Plan

 Past Tense, active or passive voice.

 Third Person perspective.
 Clear and not too wordy
 Include only what is necessary for one recreating the experiment to know.
  Materials and equipment utilized during the experiment should be mentioned throughout the
procedure as they are used.

Materials and Equipment

Preparation of Kamias Fruit Extract

One kilogram of Kamias (Averrhoa bilimbi) fruit were gathered at Sta. Maria Farms in Zamboanga City.
The fruits were then washed with distilled water and sun-dried. Using a kitchen blender, the fruits were
ground and extracted leaving behind residues. The residues were then extracted once more with the use
of cheesecloth. The Kamias fruit extract was then poured and sealed in a sterilized bottle and stored in
the refrigerator at 4 degrees C for 24 hours.

 For materials, include the exact specifications and quantities. Avoid the use of trade names –
use chemical, generic, or scientific names.
 Do not explain too much about why you have used a certain method. You can put these
explanations into your Results section. 
 Be precise. If something was heated or cooled, give the temperature.
 “How” or “how much” should be answered in your methods.
 Do not include any results.
 Make use of figures and tables, they are the most efficient way to convey bulky detailed
information.

Example

Kamias (Averrhoa bilimbi) Leaves and Fruit Extract:Its Antibacterial Effect on Escherichia coli,
Staphylococcus aureus, and Salmonella enteritidis

Materials and Equipment

Study Site

This study was conducted at the Department of Science and Technology - Zamboanga City. The
specimens of E. coli, S. aureus and S. enteritidis were obtained at the Department of Science and
Technology…

Preparation of Extracts

Kamias leaves and fruits were gathered at the EAAB (Edwin Andrews Air Base), Sta. Maria, Zamboanga
City. The leaves were then washed with distilled water and dried. Using a pair of scissors, the leaves
were cut into pieces. Using a blender, the leaves were ground into a fine powder. The resulting powder
was then extracted with the use of cheesecloth. The Kamias leaves extract was then poured and sealed
in a sterilized bottle and stored in the refrigerator at 4 degrees Centigrade for 24 hours.

Preparation of Test Organisms

The test organisms namely Escherichia coli, Staphylococcus aureus and Salmonella enteritidis were
taken from pure cultures using spread method, and were provided by the DOST-Zamboanga City. The
laboratory officer-in-charge assisted the researchers in spreading the bacteria from pure cultures to two
test tubes filled with nutrient agar. These test tubes were incubated at 35.5 degrees centigrade for 24
hours.

Treatment and General Procedures

Preparation of Treatments

The different concentrations of the leaves and fruits extracts were prepared and they were named
:Treatment A - 100% water served (negative control); Treatment B - 100% Kamias leaves extract;
Treatment C - 50% Distilled water and 50% Kamias leaves extract; Treatment D - 100% antibiotic
(positive control); Treatment E - 100% Kamias fruit extract and; Treatment F - 50% Distilled water and
50% Kamias fruit extract.

Microbiological Assay

... With the use of syringe, 0.5 mL of each bacterial specimen was transferred to its corresponding petri
dish. The specimens were spread evenly using cotton buds…

Statistical Analyses

This study utilized both descriptive and inferential statistics in analyzing and interpreting the data. Mean
was chosen as the measure of central tendency. Analysis of Variance (ANOVA) and Scheffe’s Test were
conducted to analyze and interpret the results.

For the Introduction parts, please refer to the DepEd-IntelFormat1 PowerPoint presentation.