Test of Proteins Description Reagent Procedure Product Responsible Positive Result

for color
Denaturation is the In 3 test tubes, place 3 White, cloudy, milky,
unfolding of the mL of egg albumin The presence of the coagulate at the top of
complex secondary, solution. To test tube 1, heavy metal salts the solution; insoluble
tertiary, and  Lead Acetate add 5 drops of lead which denature the metalloprotein
quaternary structure acetate solution. Add proteins
 Silver Nitrate
Protein of proteins. Heavy excess drops of lead
Denaturation by metal ions denature  Mercuric acetate solution and
Precipitation proteins by combining note whether the
Chloride
(Heavy metal the free carboxylate precipitate is increased
salts) anions of the acidic or dissolved. Repeat the
amino acid with the procedure by adding 5
metal, causing drops of silver nitrate to
precipitation test tube 2 and 5 drops
of mercuric chloride.
Describe the results.
Organic acids are also Place 3 mL of egg The presence of the Yellow to yellow-white
used to precipitate the albumin solution in 2 test organic acids which coagulate; insoluble
alkaloids giving rise to tubes. In test tube 1, disrupt the salt salt complex with
the name “alkaloidal  Tannic Acid add 2 mL of tannic acid linkages, causing the protein
reagents”. The anion solution and in test tube precipitation
 Picric Acid
Protein of the acid will react 2, add 2 mL of picric acid
Denaturation by with the protonated solution. Describe the
Precipitation amino groups of protein solution in each
(Alkaloidal protein, causing of the test tubes after
Reagents) disruption of the salt the addition of alkaloidal
linkages. reagents.