Methanolic plant residue dilution chart needed for DPPH

Wt of Wt of evaporating Wt of Wt of Wt of Dilution needed to

evaporating dish with ethanolic ethanolic ethanolic prepare 10mg/ml
dish methanolic residue residue/5ml residue/ml residue/ml
g/5ml g/ml mg/ml
Cv 93.048 93.205 0.157 0.0314 31.4 9.68 0.32
Csim 45.966 46.049 0.083 0.0166 16.6 9.4 0.6
Cg 54.348 54.617 0.269 0.0538 53.8 9.81 0.19
Csp 37.67 37.733 0.063 0.0126 12.6 9.21 0.79
Cc 53.948 53.997 0.049 0.0098 9.8 8.98 1.02
Ta 106.648 106.693 0.045 0.009 9 8.89 1.11
Pl 80.617 80.657 0.04 0.008 8 8.75 1.25
Cy 83.504 83.641 0.137 0.0274 27.4 9.64 0.36
As 93.227 93.263 0.036 0.0072 7.2 8.61 1.39
Sample Methanol Sample ml
extract ml
Conc.Of Standard Ascorbic acid10mg/ml and Extracts 1mg/ml

DPPH (2,2-diphenyl-1-picrylhydrazyl) reagent = 2.4mg in 45ml methanol

DPPH OD =

Sample Name =Cleome simplicifolia (Camb.) Hook f and Thoms

OD at 517nm OD at 517nm
Sample METHANOL DPPH
STD 1mg/ml extract

10mg/ml 10 mg/ml Set 1 Set 2 Set 3

0 1 3 0.0 0.0 0.0 0.0 0.0

0.1 0.9 3 0.422 0.411

0.2 0.8 3 Incubate 0.356 0.353

for 30min
0.3 0.7 3 at DARK 0.195 0.201

0.4 0.6 3 0.101 0.098

0.5 0.5 3 0.051 0.043

Conc.Of Standard Ascorbic acid10mg/ml and Extracts 1mg/ml

DPPH (2,2-diphenyl-1-picrylhydrazyl) reagent = 2.4mg in 45ml methanol

DPPH OD =

Sample Name =Cleome viscosa L.

OD at 517nm OD at 517nm
Sample METHANOL DPPH
STD 1mg/ml extract

10mg/ml 10 mg/ml Set 1 Set 2 Set 3

0 1 3 0.0 0.0 0.0 0.0 0.0

0.1 0.9 3 0.422 0.411

0.2 0.8 3 Incubate 0.356 0.353

for 30min
0.3 0.7 3 at DARK 0.195 0.201

0.4 0.6 3 0.101 0.098

0.5 0.5 3 0.051 0.043

Conc.Of Standard Ascorbic acid10mg/ml and Extracts 1mg/ml

DPPH (2,2-diphenyl-1-picrylhydrazyl) reagent = 2.4mg in 45ml methanol

DPPH OD =

Sample Name =Cleome gynandra

OD at 517nm OD at 517nm
Sample METHANOL DPPH
STD 1mg/ml extract

10mg/ml 10 mg/ml Set 1 Set 2 Set 3

0 1 3 0.0 0.0 0.0 0.0 0.0

0.1 0.9 3 0.422 0.411

0.2 0.8 3 Incubate 0.356 0.353

for 30min
0.3 0.7 3 at DARK 0.195 0.201

0.4 0.6 3 0.101 0.098

0.5 0.5 3 0.051 0.043

Conc.Of Standard Ascorbic acid10mg/ml and Extracts 1mg/ml

DPPH (2,2-diphenyl-1-picrylhydrazyl) reagent = 2.4mg in 45ml methanol

DPPH OD =

Sample Name =Cleome chelidonii

OD at 517nm OD at 517nm
Sample METHANOL DPPH
STD 1mg/ml extract

10mg/ml 10 mg/ml Set 1 Set 2 Set 3

0 1 3 0.0 0.0 0.0 0.0 0.0

0.1 0.9 3 0.422 0.411

0.2 0.8 3 Incubate 0.356 0.353

for 30min
0.3 0.7 3 at DARK 0.195 0.201

0.4 0.6 3 0.101 0.098

0.5 0.5 3 0.051 0.043

Conc.Of Standard Ascorbic acid10mg/ml and Extracts 1mg/ml

DPPH (2,2-diphenyl-1-picrylhydrazyl) reagent = 2.4mg in 45ml methanol

DPPH OD =

Sample Name =Cleome speciosa

OD at 517nm OD at 517nm
Sample METHANOL DPPH
STD 1mg/ml extract

10mg/ml 10 mg/ml Set 1 Set 2 Set 3

0 1 3 0.0 0.0 0.0 0.0 0.0

0.1 0.9 3 0.422 0.411

0.2 0.8 3 Incubate 0.356 0.353

for 30min
0.3 0.7 3 at DARK 0.195 0.201

0.4 0.6 3 0.101 0.098

0.5 0.5 3 0.051 0.043

Conc.Of Standard Ascorbic acid10mg/ml and Extracts 10mg/ml

DPPH (2,2-diphenyl-1-picrylhydrazyl) reagent = 2.4mg in 45ml methanol

DPPH OD =

OD at 517nm
Plant name Sample METHANOL DPPH 10mg/ml extract
Set 1 Set 2 Set 3
0 1 3
0.1 0.9 3
0.2 0.8 3 Incubate
for 30min
0.3 0.7 3 at DARK
0.4 0.6 3
0.5 0.5 3

0 1 3
0.1 0.9 3
0.2 0.8 3 Incubate
for 30min
0.3 0.7 3 at DARK
0.4 0.6 3
0.5 0.5 3

0 1 3
0.1 0.9 3
0.2 0.8 3 Incubate
for 30min
0.3 0.7 3 at DARK
0.4 0.6 3
0.5 0.5 3
Conc.Of Standard Ascorbic acid10mg/ml and Extracts 10mg/ml

DPPH (2,2-diphenyl-1-picrylhydrazyl) reagent = 2.4mg in 45ml methanol

DPPH OD =

OD at 517nm
Plant name Sample METHANOL DPPH
10mg/ml extract
Set 1 Set 2 Set 3
0 1 3
0.1 0.9 3
0.2 0.8 3 Incubate
for 30min
0.3 0.7 3 at DARK
0.4 0.6 3
0.5 0.5 3

0 1 3
0.1 0.9 3
0.2 0.8 3 Incubate
for 30min
0.3 0.7 3 at DARK
0.4 0.6 3
0.5 0.5 3
Conc.Of Standard Ascorbic acid10mg/ml and Extracts 10mg/ml

DPPH (2,2-diphenyl-1-picrylhydrazyl) reagent = 2.4mg in 45ml methanol

DPPH OD =

OD at 517nm
Plant name Sample METHANOL DPPH 10mg/ml extract

Set 1 Set 2 Set 3

0 1 3

0.1 0.9 3 Incubate

0.2 0.8 3 for 30min
0.3 0.7 3 at DARK
0.4 0.6 3
0.5 0.5 3