Академический Документы
Профессиональный Документы
Культура Документы
in tissue
11h January 2007
Material to cover
• Photothermal
• Photoablation
• Photon transport in tissue.
1
Light-Tissue Interaction
Note:
Optical Modification
1-1000 J g-1
Ionizing Radiation
50 Grey 0.05 J g-1
Diagnostic Threshold
S1
T1
Are other types of interactions
available at very short pulse
S0 durations?
2
Resonance vs. multiphoton absorption
Determining Parameters
• Power density [Wcm-2] Irradiance or Fluence
rate
• Energy density [Wcm-2] Radiant Exposure or
Fluence
• Time [sec] Time
• Absorption coefficient
[cm-1] Extinction, Attenuation
3
Phototherapeutics
Thermal IR, vis
Chemical UV, vis (NIR)
}
Dissociation UV
Mechanical all need high power
Ionization all
ln Φ (d ) ln Φ (d )
µ eff ln Φ (d ) µ eff ln Φ (d )
d d
4
Light penetration and local
absorption with scattering
High Quantum
Energry Low Quantum Energy
Linear addition Photooxidation
Substitution reactions Cis-trans isomeration
Cycloadditions
Fragmentation
5
Photochemical
• PDT
• Laser Biostimulation
(not enough science for 2 hours)
6
H&E micrographs of plantar aspect of the femoro-tibial joint sections, with the femur on the
right side of each image. A: Five hours after injection of 10 ul of PBS solution demonstrating
absence of cells in the joint. B: Ten microliters of PBS containing 300 lg of zymosan A
without LLLT with presence of granulocytes in the joint space. C: Five hours after zymosan A
injection followed by635 nm–mediated LLLT. D, E: Twenty-four hours after zymosan A
injection without LLLT (D) and at 24 h with LLLT (E), respectively.
Photothermal Effects
dT 1
= {(η H µ a Φ d ) − RL }
dt Cv ρ
Specific heat
η H µ a Φ d = RH
Fluence rate
Fraction converted
into heat
Rate of heat loss
7
Pulse duration in Thermal effects
8
Photothermal
• Skin resurfacing
• Selective
Photothermolysis
• Tissue
welding/modification
9
Clinical Examples:Port wine stain
haemangioma, scars
Tissue ablation
Goals Physical requirements
• Rapid removal of • Maximize
undesired tissue vaporization of water
• Prevention of open • Retaining a
wound coagulation zone
• Rapid healing • Minimal coagulation
zone
• Consider mechanical
properties of tissue
10
Photoablative
• Ablation
• Vascular re-canalization
(myocardium perfusion)
• LASIK
• Pulsed Photothermal
Radiometry
11
Photoablative Materials processing
in non-biological materials
Aluminum
substrate
12
Mechanical properties
of tissue
Cavity
Shockwave
13
e.g. corneal reshaping
PRK
LASIK
14
Soft tissue application
15
Approach for medical therapy
investigations
• Cellular component
only to investigate
response of genetic
content to
– Plasma
– Shockwave
– Soft x-ray
16
9L glioma spheroid after
10nsec 1064 nm
17
Scattering
• Rayleigh
S 1 + cos 2 ϕ 4 2
≈ ωα N=
1 + cos 2 ϕ 4 n 2 − 1
ω
(
ε02
)
2
2
S0 r r2 N
http://www-phys.llnl.gov/Research/scattering/
•Mie
http://scienceworld.wolfram.com/physics/MieScattering.html
18
Towards Light Transport in
Tissue
∫ p(s, s')dω = 1
4π
Energy density
Scattering phase function, Isotropic
g= ∫ p(s, s')(s, s')dω
4π
scattering
Heney-Greenstein function
g = (1 − β )g HG
1 1 − g HG
2
p HG (cos Ω ) = β + (1 − β )
4π
(
1 + g HG − 2 g HG cos Ω
2 1.5
)
19
Heney-Greenstein function
20
Diffusion Equation
∇ 2ϕ d (r ) − 3µ t 'ϕ d (r ) + 3µ t ' µ s E (r , s0 ) − 3µ s g∇(E (r , s0 )s0 ) = 0
Semi-infinite medium
21
Monte Carlo Methods
Advanced Models
22
Sample Applications:
Photocoagulation of vessels
a'
Rtotal =
3k µs'
1 + 2k (1 − a ') + 1 + 3(1 − a ')
a' =
(1 − a ' ) µa + µs'
23
Analytical models for R
and R(ρ’) but also
numerical.
Inverse solution using
NN or Lookup tables.
µ s ' = µ s (1 − g )
µ eff = 3µ a (µ a + µ s ' )
µs'
a' =
µa + µ s'
24
25
Time or Frequency Dependent:
Reflectance/Transmittance
c=c0 / n n=1.35-1.45
26
−ρ 2
R( ρ , t ) = 4πDc 3 z0t e (− µ a ct )e
4 Dct
d ln R( ρ , t ) 5 ρ2
= − t − µac +
dt 2 4 Dct 2
d ln R( ρ , t )
∴ lim t → ∞ = −µac
dt
φ (ρ , f ) = π (µ eff ρ 0 )2
f
(1 + µ eff ρ 0 )µ a c
ρ 0 2 = ρ 2 + z0 2
27
time frequency
Advantage •Direct little •Fast
interpretation •Cheaper technology
•Complete time
profile data
Disadvantage •Slow data •Data interpretation
collection obscure
•Requires fast •Varied ρ required
pulses high cost •High f expensive
single electronics
wavelenght •Modulations of
lasers and or lamps
28