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Background
F etal Bovine Serum (FBS) is a growth-promoting
supplement used in tissue culture for virtually all types
of cells and is also known as foetal bovine serum, and
Box 1 | Basic Info
fetal/foetal calf serum (FCS). FBS is a complex mixture of Cat No. Qty
nutritional, growth, hormonal, and attachment (anti-trypsin
ABP-MED-FBS0050 50 mL
activity) factors that are essential for cell propagation.
These include a wide variety of proteins, lipids, salts, ABP-MED-FBS0500 500 mL
minerals, and amino acids. Major proteins include albumin,
fetuin, fibronectin, globulins, and transferrin. Furthermore, Storage: Upon receipt, store frozen FBS at -20°C (or
FBS also appears to serve as a protective buffer against below) protected from light; Do not allow it to thaw until
detrimental changes, such as pH differences, heavy ready for use. FBS should last more than five years
metals, proteolytic activity, and endotoxin. Other types of under these conditions. Please aviod more than one
sera include calf serum (CS), and horse serum. Raw sera Freeze/Thaw cylce.
are collected by aseptic cardiac puncture at the abattoir by
a licensed veterinarian. It is quickly refrigerated, and after
allowing sufficient time (usually 24 hours) for clotting, the In general, normal processing using sterile filtration (0.1
raw sera are centrifuged to remove blood cells. It is then um) will not effectively remove viruses. Several companies
pre-filtered (submicron filtered), and finally sterile-filtered have developed smaller pore filters (40 nm) which they
through a series of 0.1 um filters (at least three times). claim will remove viruses or reduce viral loads. This is an
Processing is carefully performed at 4°C to minimize option if you have virus concerns. Note that some larger
product degradation, and the FBS is immediately frozen proteins (e.g., IgM) may also be removed by these smaller
after processing. pore filters.
Box 2 | Specifications
PH pH meter 6.9-7.8