Code No: RR422301 Set No.

1
IV B.Tech II Semester Supplimentary Examinations, May 2008
CHROMATOGRAPHIC SEPARATIONS
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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1. Write short notes on the following:

(a) Retention time

(b) Elution volume
(c) Capacity factor
(d) Plate height. [4×4=16]

2. Describe one method for the development of chromatogram and write a note on
column resolution. [16]

3. Briefly outline the advantages which may be gained by preparing specific ion ex-
change resins which contain functional groups known to react selectively with par-
ticular groups of cations. [16]

4. (a) How adsorbents are selected in TLC?

(b) Discuss the limitations of TLC. [8+8]

5. (a) Write briefly procedure for column packing.

(b) Write a short note on pumping systems used in HPLC. [8+8]

6. Write Short notes on:

(a) PLOT
(b) Chromatogram
(c) Electron capture detector
(d) Properties of mobile phase in GC. [4×4=16]

7. (a) Write about the types and interactions between chiral resolving agent and the
solute.
(b) Write the industrial applications of chiral chromatography. [8+8]

8. Write the principle of capillary electrophoresis.Write about various types of capil-

lary electrophoretic operations. [16]

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Code No: RR422301 Set No. 2
IV B.Tech II Semester Supplimentary Examinations, May 2008
CHROMATOGRAPHIC SEPARATIONS
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Write short notes on the following:

(a) Solvent extraction
(b) Solid-Phase extraction
(c) Column switching
(d) Super critical extraction. [4×4=16]
2. Describe one method for the development of chromatogram and write a note on
column resolution. [16]
3. (a) List out the commonly used ion exchangers and write matrices used for them.
(b) What are the factors that influence the ion exchange chromatography perfor-
mance? [8+8]
4. (a) Write a short notes on spot densitometer.
(b) Describe the application of TLC for separation of inorganic ions. [8+8]
5. (a) Discuss the fraction collectors in HPLC system.
(b) Write a short note a Evaporative light scattering detector (ELSD) AND NMR
spectrometer detector. [8+8]
6. Write the characteristics, advantages and limitations of various detectors used in
Gas Chromatography. [16]
7. Write short notes on:
(a) Enantiomers
(b) Chiral resolving agent
(c) Retention time
(d) Band broadening. [4×4=16]
8. (a) Write the principle of separation by capillary zone electrophoresis.
(b) A inorganic cation has an electrophoretic mobilities of 4×10−4 cm−2 s−1 v−1 .This
same ion has diffusion coefficient of 10×10−6 cm2 .s−1 . If this ion is separated
by capillary zone electrophoresis with 50cm capillary,calculate the experted
plate count N at appliled voltage of 10kv. [8+8]

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Code No: RR422301 Set No. 3
IV B.Tech II Semester Supplimentary Examinations, May 2008
CHROMATOGRAPHIC SEPARATIONS
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Classify and explain the chromatographic techniques with suitable examples and
schematic diagrams. [16]

2. Describe one method for the development of chromatogram and write a note on
column resolution. [16]

3. Briefly outline the advantages which may be gained by preparing specific ion ex-
change resins which contain functional groups known to react selectively with par-
ticular groups of cations. [16]

4. (a) Write a short notes on spot densitometer.

(b) Describe the application of TLC for separation of inorganic ions. [8+8]

5. What is the design principle of atypical HPLC machine? Discuss with suitable
equation. [16]

6. Write about the stationary phases commonly used in Gas chromatography. [16]

7. (a) Discuss different types of stationary phases used in chiral chromatography.

(b) Explain the nature of chiral resolving agent in chiral chromatography. [8+8]

8. Write about electro osmotic flow and pressure induced flow in capillary electrophore-
sis. [16]

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Code No: RR422301 Set No. 4
IV B.Tech II Semester Supplimentary Examinations, May 2008
CHROMATOGRAPHIC SEPARATIONS
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Write short notes on the following:

(a) Solvent extraction

(b) Solid-Phase extraction
(c) Column switching
(d) Super critical extraction. [4×4=16]

2. Differentiate between the photouiode array detector and fluorescence detector with
suitable diagrams. [16]

3. (a) List out the commonly used ion exchangers and write matrices used for them.
(b) What are the factors that influence the ion exchange chromatography perfor-
mance? [8+8]

4. Write applications of size exclusion chromatography technique. [16]

5. What is the hydrophobic interaction chromatography? Explain with a neat dia-

gram. [16]

6. What is a chromatogram? Describe the physical differences between open tubular

and packed columns. [16]

7. (a) What are various chiral mobile phase additives used in chiral chromatography?
(b) Write the principles separation of racemic mixture in chiral chromatography.
[8+8]

8. Write Short notes on:

(a) Capillary array electrophoresis

(b) Isoelectric focusing
(c) Capillary zone electrophoresis
(d) Electro osmotic flow. [4×4=16]

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