Eo (g/L) T (°C) I (mmol/ml) S (mmol/ml) V (mmol/ml-min)

1.6 30 0 0.1 2.63

1.6 30 0 0.033 1.92
1.6 30 0 0.02 1.47
1.6 30 0 0.01 0.96
1.6 30 0 0.005 0.56

1/S (mmol/ml) 1/V (mmol/ml-min)

10 0.38022813688213
30.303030303 0.52083333333333
50 0.68027210884354
100 1.04166666666667
200 1.78571428571429

1.8
f(x) = 0.007407984030173 x + 0.303471183205387
1.6 R² = 0.999916158743922

1.4

1.2

0.8

0.6

0.4

0.2

0
0 50 100 150 200 250
a= 0.0074
b= 0.3035
r= 0.9999

Vmax = 135.1351 mmol/ml-min