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Proponents:
Godard Barbosa
Adviser:
A. General Objective:
•To determine the effect of different substrates on the biodegradability
of SM plastic bags.
B.Specific Objectives:
1. To expose SM plastic bags to different substrates such as compost soil,
Loam soil, seawater and tap water for a duration of several months.
2. To measure the weight of the plastic bags (grams) before and after
Different exposure periods to the substrates.
3. To compare the percent weight loss of the SM plastic bags exposed to
Different substrates after a period of time
A. Variables
A1. Independent variables/ manipulated variable: substrate
1. Compost soil
2. Loam soil
3. Seawater
4. Tap water
B. Materials
•6, 1-litre glass jars
•6, 7”x9” aluminum trays
•12 pcs. 3”x4” SM plastic bags
•500g loam soil
•500g compost soil
•500mL seawater
•500mL tap water
•Analytical balance
•Graduated cylinder
•Weighing scale
C. Procedures
We cut the SM plastic bags into 3”x4”sizes strips. We labelled theplastic strips into C1,
C2, C3 for compost soil, L1, L2, L3 for loamsoil, S1, S2, S3 for seawater, and T1, T2, T3
for tap watertreatments. We then determined the initial weights of the plasticstrips in
grams using the analytical balance. The initial weights (W1)of the plastic strips were then
recorded in our log book.
Percent weight loss in each plastic strip was determined using the
Formula:
Days after the plastics were submerged tothe different substrates, the plastic strips were
harvested to observe any physical change. No physical change was observed in any of the
strips. A month after the immersion, the plastic strips were again observed. No physical
change was seen in any of the plastic strips.
Days after the plastic strips were immersed; they were harvested, washed with tap water
and air dried for 48 hours. This time, almost all the strips showed physical changes such
as the appearance of small holes, thinning in some portion, and discoloration.
Summary of Results:
Weight loss was observed after about two month’s exposure to different soil and water
medium. Percent weight loss ranged from 0.09% to 30.28%.Weight loss was higher in
plastic strips submerged in seawater and compost soil. This was followed by weight loss
in plastic strips submerged in loam soil. Weight loss was lowest for plastic strips
submerged in tap water. Mean weight loss for all was plastic strips was 12.63% (Table 1).
No physical changes were observed in all plastic strips on September24, 2008, 16 days
after exposure to different substrates. Likewise, no physical changes were observed on
the plastic strips. After 28 days of exposure. After 68 days of exposureto the different
substrates, physical changes were observed in all plastic strips. Some plastic strips have
small holes. Other portions of the strips have thinned out, while some showed
discoloration (Table 2, Plates 7, 8, and 9).
Results showed that after about two months exposure to loam soil, compost soil, seawater
and tap water, the SM plastic strips manifested signs of degradation such as weight loss,
changes in physical appearance like tearing, thinning and discoloration. However, it is
too early to tell whether the degradation observed are due to physical and chemical
factors such as temperature, moisture, and enzymatic reactions, or these are due to the
action of microorganisms like bacteria (biodegradation).
After observing the physical changes and measuring the dry weight of the plastic strips,
these were again returned to the different treatment substrates for further observations.