Code No: RR322302 Set No.

1
III B.Tech II Semester Supplimentary Examinations, Apr/May 2008
ANALYTICAL TECHNIQUES IN BIOTECHNOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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1. Write the principles involved in construction of a Phase Contrast Microscope. [16]

2. Explain

(a) Cryopreservation
(b) Numerical aperture. [8+8]

3. (a) Distinguish between Nucleotides and Nucleosides

(b) Deoxyribose and ribose [8+8]

4. What are the factors affecting electrophoretic mobility of any molecule? [16]

5. Explain the difference between Column Chromatography and Planar Chromatog-

raphy. [16]

6. What is meant by in-situ PCR? Where is it useful? [16]

7. Explain how Immunoprecipitation property has been exploited? [16]

8. What is meant by the term “Biomass”? Explain its relevance to cell growth. [16]

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Code No: RR322302 Set No. 2
III B.Tech II Semester Supplimentary Examinations, Apr/May 2008
ANALYTICAL TECHNIQUES IN BIOTECHNOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Write the principles involved in construction of a Phase Contrast Microscope. [16]

2. Why can’t biological specimens be viewed under an electron microscope without

any prepautive steps? [16]

3. (a) Distinguish between Nucleotides and Nucleosides

(b) Deoxyribose and ribose [8+8]

4. How is pulse field Electrophoresis done? [16]

5. Discuss the importance of Elution time; volume and Capacity factor in Chromatog-
raphy. [16]

6. What are automated DNA sequencers? [16]

7. What is the principle involved behind RIA? [16]

8. Discuss product formation kinetics in cell culture. [16]

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Code No: RR322302 Set No. 3
III B.Tech II Semester Supplimentary Examinations, Apr/May 2008
ANALYTICAL TECHNIQUES IN BIOTECHNOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Write in detail the description of a typical microbiological light microscope. [16]

2. What do you mean by specimen preparation of TEM? [16]

3. What are proteins? Give a few examples of proteins present in living matters.[16]

4. List the difference between 1D and 2D Electrophoresis. [16]

5. Write short notes on

(a) Paper chromatography

(b) Thin layer chromatography. [8+8]

6. What are the differences between Monoclonal antibodies and Polyclonal antibodies?
[16]

7. What is meant by Biotinylation of antibodies? [16]

8. What is the difference between BOD and COD? [16]

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Code No: RR322302 Set No. 4
III B.Tech II Semester Supplimentary Examinations, Apr/May 2008
ANALYTICAL TECHNIQUES IN BIOTECHNOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Write in detail the description of a typical microbiological light microscope. [16]

2. What do you mean by specimen preparation of TEM? [16]

3. How is blood sugar measured? [16]

4. What is PAGE? [16]

5. Discuss the importance of Elution time; volume and Capacity factor in Chromatog-
raphy. [16]

6. Explain antisera production in detail. [16]

7. Write short notes on

(a) FACS
(b) Anti - antibodies. [8+8]

8. Explain

(a) Substrate uptake in absence of product formation.

(b) Substrate uptake with product formation. [8+8]

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